Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0 0 0 – 0 0 0 0 0 0.07 0.09 0 0.16 0.11 0.1 0 0.07 0 0.07 0.06 0.07
Bud 0 0 0.07 0.05 0 0 – 0.06 0 0.06 0.05 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 – 0 0 0 0 0 0.06 0.05 0 0.13 0.06 0 0 0 0 0 0 0
Cytoplasm 0.16 0.14 0.12 0.06 0.08 0.12 – 0.13 0.12 0.11 0.14 0.14 0.12 0.22 0.36 0.15 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.12 0.05 0 0 0 – 0 0 0 0 0 0.13 0.15 0.17 0.05 0.09 0 0.05 0 0 0 0 0.05
Endosome 0.13 0 0 0 0.18 0 – 0.05 0 0 0 0 0.1 0.08 0.1 0.11 0 0.06 0.09 0.09 0.09 0.07 0.1 0.1
Golgi 0 0.1 0 0 0 0 – 0 0 0 0 0 0.24 0.13 0.06 0.18 0.29 0.39 0.06 0.09 0 0.13 0.11 0.09
Mitochondria 0.57 0.54 0.86 0.94 0.73 0.88 – 0.84 0.91 0.91 0.89 0.9 0.41 0.26 0.16 0.11 0.2 0.13 0.52 0.55 0.65 0.51 0.41 0.39
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.22 0.08 0.05 0.14 0.06 – 0 0 0 0.05 0 0.2 0.19 0.2 0.33 0.29 0.24 0.12 0.07 0.05 0.08 0.13 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 11 0 1 0 0 – 0 0 0 0 0 15 18 8 9 4 7 16 15 4 12 17 18
Bud 3 2 11 10 8 9 – 23 13 25 17 20 2 2 0 0 2 3 12 2 0 3 7 8
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 9 2 4 2 5 7
Bud Site 0 0 0 0 0 1 – 7 1 7 5 7 0 1 0 0 0 0 – – – – – –
Cell Periphery 7 5 0 10 3 1 – 0 1 1 0 3 13 10 10 7 2 3 3 0 0 1 0 0
Cytoplasm 56 23 18 11 20 29 – 51 40 46 50 48 26 45 85 8 4 6 15 2 3 1 6 10
Endoplasmic Reticulum 25 20 7 2 7 9 – 3 4 0 5 4 28 31 40 3 3 3 21 8 3 3 12 13
Endosome 45 7 3 1 47 3 – 21 2 4 0 0 22 16 23 6 1 4 43 20 14 12 28 26
Golgi 9 16 0 0 1 1 – 0 0 0 0 0 52 27 15 10 10 26 27 19 4 22 31 26
Mitochondria 199 88 126 178 187 206 – 339 295 364 312 309 88 52 38 6 7 9 244 119 105 86 116 108
Nucleus 2 0 2 0 0 1 – 7 4 10 2 4 1 0 1 1 0 0 1 0 0 0 1 1
Nuclear Periphery 0 1 0 0 0 0 – 4 4 3 0 1 0 0 0 0 0 0 4 1 0 1 0 3
Nucleolus 0 0 2 0 2 2 – 5 1 5 5 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 1 0 0 11 1 – 2 2 4 0 3 0 1 1 1 0 0 2 3 3 4 9 2
SpindlePole 2 1 1 0 3 2 – 11 6 16 9 17 0 3 0 3 1 4 2 0 3 1 4 1
Vac/Vac Membrane 75 36 12 9 35 14 – 18 13 14 16 5 44 38 47 18 10 16 55 15 8 14 38 43
Unique Cell Count 350 163 147 189 255 233 402 325 402 349 345 217 201 238 55 35 67 469 216 163 170 286 279
Labelled Cell Count 438 211 182 222 324 279 491 386 499 421 422 291 244 268 72 45 82 469 216 163 170 286 279


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.6 4.6 5.3 6.2 4.2 – 4.0 3.5 3.6 3.4 3.4 7.5 7.3 6.5 9.8 9.2 9.0 7.2 7.5 7.4
Std Deviation (1e-4) 1.9 1.0 1.1 1.1 2.2 1.1 – 1.7 1.4 1.0 1.0 1.4 1.1 1.1 1.0 2.3 1.5 1.3 1.2 1.6 1.9
Intensity Change (Log2) – – – 0.21 0.42 -0.12 – -0.19 -0.4 -0.38 -0.45 -0.44 0.71 0.66 0.48 1.09 1.0 0.97 0.64 0.71 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 3.3 3.7 0 0 0 0
Bud -0.8 -2.0 0 – 0 0 0 0 0 -3.3 -3.2 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 3.0 0 0 0 0 0
Cytoplasm -2.1 -1.5 0.1 – 0.1 0 -0.3 0.6 0.5 -0.1 2.4 5.1 0.4 0 -0.7
Endoplasmic Reticulum 0 -1.1 0 – 0 0 0 0 0 2.6 3.1 3.5 0 0 0
Endosome 0 4.8 0 – 0 0 0 0 0 3.0 2.4 2.9 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 6.4 4.6 3.1 0 0 8.1
Mitochondria 2.6 -2.9 0 – 0 0 0 0 0 -8.6 -11.0 -13.4 -9.9 -7.9 -10.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 1.7 -0.8 – -1.7 -1.9 -2.3 -1.6 -3.7 3.1 2.8 3.1 4.4 0 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2297 3.2593 3.0508 2.5697 2.653 3.1437 2.7624 3.0874 2.8247 2.6577 2.4685 2.9053 4.0228 4.6765 4.3015 4.1456 3.8174 4.5159
Actin 0.0291 0.036 0.0359 0.0053 0.033 0.0218 0.0365 0.0469 0.0347 0.0343 0.0288 0.0173 0.0394 0.0371 0.0473 0.0063 0.0629 0.0068
Bud 0.0028 0.0055 0.0024 0.0087 0.0099 0.0037 0.0031 0.0054 0.0054 0.0043 0.0036 0.002 0.0048 0.0057 0.006 0.003 0.0046 0.0024
Bud Neck 0.0023 0.0029 0.0058 0.0067 0.0016 0.0104 0.002 0.0024 0.0044 0.0018 0.0014 0.0081 0.0021 0.0022 0.0022 0.0108 0.0028 0.0017
Bud Periphery 0.0046 0.006 0.0035 0.0531 0.0198 0.0081 0.0048 0.0069 0.01 0.0074 0.0036 0.0045 0.0085 0.007 0.0081 0.0046 0.0049 0.0068
Bud Site 0.0108 0.0449 0.0126 0.0047 0.0065 0.0048 0.0121 0.0619 0.0306 0.0106 0.0025 0.002 0.0141 0.0639 0.0223 0.0056 0.0064 0.0014
Cell Periphery 0.0038 0.0019 0.0027 0.0026 0.0019 0.0015 0.0016 0.0024 0.0038 0.0019 0.0005 0.0014 0.002 0.0031 0.0028 0.0006 0.0006 0.0009
Cytoplasm 0.0372 0.0589 0.0369 0.029 0.0188 0.0393 0.0345 0.0771 0.0696 0.0207 0.0274 0.0238 0.0192 0.0957 0.0786 0.0158 0.0182 0.021
Cytoplasmic Foci 0.0509 0.0993 0.0639 0.081 0.0801 0.0544 0.0669 0.0765 0.062 0.0436 0.078 0.0448 0.0599 0.0766 0.0764 0.0564 0.0745 0.04
Eisosomes 0.0006 0.0006 0.0021 0.0003 0.0006 0.0004 0.0007 0.0008 0.0007 0.0003 0.0003 0.0005 0.0007 0.0008 0.0009 0.0003 0.0004 0.0005
Endoplasmic Reticulum 0.0137 0.0083 0.0104 0.0024 0.005 0.0103 0.0131 0.0061 0.0093 0.003 0.0016 0.0081 0.0073 0.0178 0.0148 0.0017 0.0016 0.0127
Endosome 0.2202 0.1905 0.1413 0.2365 0.107 0.157 0.2147 0.1693 0.1855 0.1531 0.1805 0.1762 0.2229 0.1767 0.1782 0.1727 0.1352 0.1773
Golgi 0.148 0.1844 0.1859 0.1107 0.1302 0.1529 0.1572 0.1662 0.1806 0.14 0.2648 0.1594 0.1616 0.1438 0.1788 0.1156 0.1714 0.1828
Lipid Particles 0.012 0.0099 0.017 0.0061 0.0154 0.004 0.0224 0.003 0.0059 0.0063 0.0181 0.0027 0.0126 0.0076 0.0115 0.0186 0.1323 0.0052
Mitochondria 0.3805 0.2946 0.43 0.4154 0.5322 0.4969 0.3757 0.3495 0.3581 0.5514 0.3673 0.5292 0.4104 0.3251 0.3386 0.5524 0.3611 0.5146
None 0.0167 0.0177 0.0055 0.0055 0.0022 0.0081 0.0054 0.002 0.0028 0.001 0.0004 0.0011 0.0017 0.0083 0.0023 0.0012 0.0004 0.0008
Nuclear Periphery 0.0129 0.0016 0.0028 0.0006 0.0083 0.0012 0.0087 0.0007 0.0023 0.0006 0.0004 0.0007 0.0014 0.0023 0.0033 0.0005 0.0003 0.001
Nucleolus 0.0012 0.001 0.0014 0.0003 0.0007 0.0002 0.0006 0.0003 0.0004 0.0002 0.0001 0.0001 0.0003 0.0004 0.0003 0.0002 0.0002 0.0001
Nucleus 0.0059 0.0021 0.0027 0.0007 0.0012 0.0007 0.0027 0.0008 0.0024 0.0005 0.0003 0.0005 0.0009 0.0012 0.001 0.0004 0.0003 0.0004
Peroxisomes 0.0163 0.0157 0.021 0.0137 0.0076 0.0116 0.0106 0.0061 0.0075 0.0072 0.0129 0.0056 0.0102 0.0064 0.012 0.0203 0.0155 0.0094
Punctate Nuclear 0.0068 0.0008 0.0032 0.0009 0.002 0.0003 0.0022 0.0003 0.0006 0.0003 0.0003 0.0001 0.0008 0.0005 0.0004 0.0003 0.0005 0.0001
Vacuole 0.0152 0.0132 0.0081 0.0106 0.0098 0.007 0.0142 0.0114 0.016 0.006 0.0037 0.0065 0.0125 0.0124 0.0092 0.0066 0.003 0.007
Vacuole Periphery 0.0086 0.0044 0.0048 0.0053 0.0062 0.0056 0.0102 0.0041 0.0071 0.0055 0.0036 0.0052 0.0067 0.0057 0.0048 0.0063 0.0028 0.0071

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.9214 26.187 15.1137 23.9786 23.2105 29.2777 25.0066 24.8203 30.0579 33.917
Translational Efficiency 1.552 1.3626 1.7169 1.5199 1.5795 2.2768 1.8087 2.6689 1.6935 1.5699

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘