Standard name | |
---|---|
Human Ortholog | |
Description | Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 5 | 2 | 4 | 2 | 2 | 5 |
Bud | 6 | 0 | 6 | 4 | 3 | 16 | 14 | 21 | – | 11 | 19 | 19 | 0 | 2 | 0 | 5 | 6 | 2 | 3 | 2 | 0 | 1 | 3 | 4 |
Bud Neck | 2 | 0 | 2 | 0 | 1 | 4 | 5 | 4 | – | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 2 | 0 | 1 | 2 | 7 | 7 | – | 10 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 1 | 1 | 1 | 1 | 2 | 1 | – | 2 | 0 | 2 | 0 | 1 | 1 | 9 | 4 | 3 | 0 | 0 | 3 | 0 | 0 | 2 |
Cytoplasm | 86 | 20 | 106 | 120 | 167 | 164 | 185 | 263 | – | 207 | 164 | 158 | 17 | 104 | 115 | 13 | 6 | 5 | 28 | 13 | 39 | 14 | 14 | 24 |
Endoplasmic Reticulum | 2 | 8 | 2 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 3 | 0 | 1 | 3 | 1 | 4 | 4 | 2 | 0 | 5 | 1 | 0 | 2 |
Endosome | 0 | 1 | 1 | 2 | 13 | 0 | 1 | 2 | – | 2 | 0 | 0 | 2 | 5 | 4 | 9 | 8 | 7 | 8 | 11 | 15 | 2 | 10 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 8 | 12 | 3 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 79 | 31 | 89 | 97 | 135 | 206 | 265 | 358 | – | 315 | 275 | 373 | 2 | 13 | 5 | 15 | 9 | 3 | 112 | 44 | 132 | 44 | 39 | 60 |
Nucleus | 1 | 0 | 0 | 0 | 3 | 7 | 5 | 5 | – | 5 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 1 | 2 | 5 | 0 | 0 | 2 | – | 1 | 0 | 1 | 0 | 2 | 0 | 17 | 9 | 4 | 2 | 0 | 3 | 1 | 1 | 1 |
SpindlePole | 3 | 0 | 10 | 6 | 17 | 13 | 19 | 42 | – | 22 | 19 | 23 | 0 | 0 | 1 | 11 | 4 | 6 | 5 | 1 | 2 | 1 | 3 | 3 |
Vac/Vac Membrane | 8 | 1 | 1 | 0 | 3 | 3 | 2 | 3 | – | 4 | 4 | 3 | 1 | 3 | 6 | 9 | 21 | 12 | 5 | 5 | 9 | 4 | 10 | 14 |
Unique Cell Count | 163 | 54 | 186 | 200 | 296 | 337 | 416 | 575 | 475 | 387 | 493 | 21 | 125 | 129 | 76 | 66 | 50 | 186 | 84 | 236 | 80 | 96 | 135 | |
Labelled Cell Count | 193 | 63 | 222 | 232 | 349 | 417 | 509 | 710 | 585 | 493 | 592 | 22 | 132 | 136 | 99 | 82 | 63 | 186 | 84 | 236 | 80 | 96 | 135 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.1 | 3.1 | 3.0 | 3.0 | 3.0 | 2.6 | 2.7 | – | 2.5 | 2.5 | 2.5 | 4.6 | 4.7 | 5.4 | 6.7 | 6.8 | 6.9 | 6.4 | 5.4 | 6.1 |
Std Deviation (1e-4) | 1.4 | 0.9 | 1.0 | 1.0 | 0.8 | 0.9 | 1.4 | 1.0 | – | 0.9 | 1.1 | 0.9 | 0.8 | 1.1 | 2.4 | 1.8 | 1.5 | 1.5 | 3.8 | 3.1 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.09 | -0.08 | -0.29 | -0.2 | – | -0.31 | -0.32 | -0.31 | 0.55 | 0.59 | 0.78 | 1.09 | 1.11 | 1.13 | 1.01 | 0.79 | 0.95 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 7.7 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4666 | -0.1862 | -0.2042 | -0.5721 | -0.7213 | -0.2527 | 1.8284 | 1.9795 | 2.0012 | 1.7386 | 1.9552 | 1.9917 | -1.1275 | -0.7671 | -0.8998 | -0.6961 | -1.2346 | -0.8551 |
Actin | 0.0224 | 0.0071 | 0.0189 | 0.0165 | 0.0144 | 0.0066 | 0.0406 | 0.0078 | 0.0141 | 0.0214 | 0.0257 | 0.019 | 0.0163 | 0.0055 | 0.0108 | 0.0128 | 0.0065 | 0.0078 |
Bud | 0.0016 | 0.0107 | 0.0019 | 0.0046 | 0.0032 | 0.0012 | 0.0019 | 0.0023 | 0.0027 | 0.0049 | 0.0098 | 0.0012 | 0.0008 | 0.003 | 0.0023 | 0.0035 | 0.0044 | 0.0008 |
Bud Neck | 0.0014 | 0.0011 | 0.0053 | 0.0014 | 0.0058 | 0.0053 | 0.005 | 0.001 | 0.0012 | 0.0354 | 0.0005 | 0.005 | 0.0014 | 0.0037 | 0.0018 | 0.0012 | 0.0021 | 0.0052 |
Bud Periphery | 0.0048 | 0.0143 | 0.0065 | 0.0159 | 0.0131 | 0.0034 | 0.0044 | 0.0045 | 0.0072 | 0.0398 | 0.0667 | 0.0047 | 0.0022 | 0.0071 | 0.0085 | 0.0198 | 0.015 | 0.0023 |
Bud Site | 0.0085 | 0.0178 | 0.0098 | 0.0031 | 0.0066 | 0.001 | 0.0167 | 0.0152 | 0.0094 | 0.0092 | 0.0031 | 0.0014 | 0.0048 | 0.025 | 0.0074 | 0.0014 | 0.0031 | 0.0016 |
Cell Periphery | 0.0012 | 0.0016 | 0.0041 | 0.0038 | 0.0012 | 0.0006 | 0.0023 | 0.0008 | 0.005 | 0.0131 | 0.0017 | 0.0015 | 0.0012 | 0.0017 | 0.003 | 0.0006 | 0.0017 | 0.001 |
Cytoplasm | 0.027 | 0.0499 | 0.0395 | 0.0434 | 0.0864 | 0.0532 | 0.0226 | 0.0216 | 0.0386 | 0.0223 | 0.0416 | 0.0191 | 0.0323 | 0.059 | 0.0412 | 0.0716 | 0.1039 | 0.0547 |
Cytoplasmic Foci | 0.1113 | 0.0837 | 0.0854 | 0.0523 | 0.0984 | 0.1019 | 0.096 | 0.0522 | 0.063 | 0.087 | 0.0686 | 0.0647 | 0.1082 | 0.1113 | 0.0773 | 0.0522 | 0.0834 | 0.102 |
Eisosomes | 0.0021 | 0.0008 | 0.0017 | 0.0008 | 0.0007 | 0.0004 | 0.0013 | 0.0004 | 0.0028 | 0.0094 | 0.0015 | 0.0012 | 0.0019 | 0.0011 | 0.0019 | 0.0006 | 0.0013 | 0.0006 |
Endoplasmic Reticulum | 0.0051 | 0.0035 | 0.0054 | 0.0031 | 0.0044 | 0.0023 | 0.0065 | 0.0009 | 0.0033 | 0.0017 | 0.0056 | 0.0013 | 0.0051 | 0.0029 | 0.0037 | 0.0024 | 0.0018 | 0.0025 |
Endosome | 0.1177 | 0.1002 | 0.0897 | 0.0985 | 0.0543 | 0.0856 | 0.1232 | 0.0561 | 0.0699 | 0.0747 | 0.1291 | 0.0609 | 0.1172 | 0.0856 | 0.0584 | 0.0538 | 0.0847 | 0.0883 |
Golgi | 0.0346 | 0.0324 | 0.0282 | 0.0314 | 0.0272 | 0.0314 | 0.0624 | 0.0539 | 0.0445 | 0.0623 | 0.0497 | 0.0571 | 0.0373 | 0.0371 | 0.0274 | 0.023 | 0.0509 | 0.0414 |
Lipid Particles | 0.0545 | 0.0209 | 0.032 | 0.0238 | 0.0103 | 0.04 | 0.0419 | 0.0034 | 0.0144 | 0.012 | 0.0079 | 0.0265 | 0.0739 | 0.0343 | 0.0214 | 0.0033 | 0.0253 | 0.0317 |
Mitochondria | 0.2655 | 0.3723 | 0.3416 | 0.4534 | 0.3185 | 0.3734 | 0.4316 | 0.6072 | 0.5295 | 0.4182 | 0.3059 | 0.5761 | 0.3527 | 0.324 | 0.4229 | 0.5268 | 0.3993 | 0.3952 |
None | 0.2509 | 0.201 | 0.2529 | 0.1557 | 0.263 | 0.1798 | 0.0595 | 0.0901 | 0.0853 | 0.0523 | 0.1398 | 0.0918 | 0.1466 | 0.2164 | 0.2295 | 0.2043 | 0.1579 | 0.1758 |
Nuclear Periphery | 0.005 | 0.003 | 0.0062 | 0.0013 | 0.0042 | 0.0049 | 0.0035 | 0.0003 | 0.0023 | 0.0004 | 0.0443 | 0.0006 | 0.0056 | 0.0011 | 0.0012 | 0.001 | 0.0152 | 0.0015 |
Nucleolus | 0.0033 | 0.0022 | 0.0026 | 0.0011 | 0.0017 | 0.0014 | 0.0013 | 0.0006 | 0.0006 | 0.0022 | 0.0005 | 0.0007 | 0.0018 | 0.0013 | 0.0013 | 0.0004 | 0.0007 | 0.0009 |
Nucleus | 0.0036 | 0.0036 | 0.0031 | 0.0022 | 0.005 | 0.0029 | 0.0085 | 0.0006 | 0.0011 | 0.0016 | 0.0163 | 0.0006 | 0.0023 | 0.0014 | 0.0014 | 0.001 | 0.0013 | 0.0012 |
Peroxisomes | 0.058 | 0.0572 | 0.0457 | 0.0666 | 0.0657 | 0.0881 | 0.0495 | 0.0745 | 0.0934 | 0.1169 | 0.068 | 0.0569 | 0.0628 | 0.0644 | 0.0639 | 0.0101 | 0.0167 | 0.0716 |
Punctate Nuclear | 0.0049 | 0.002 | 0.0031 | 0.0009 | 0.0082 | 0.0025 | 0.0011 | 0.0002 | 0.0006 | 0.001 | 0.0009 | 0.0017 | 0.004 | 0.0011 | 0.0014 | 0.0006 | 0.0033 | 0.0051 |
Vacuole | 0.0116 | 0.0114 | 0.0112 | 0.0132 | 0.0055 | 0.0093 | 0.0133 | 0.0043 | 0.007 | 0.0092 | 0.0077 | 0.0048 | 0.015 | 0.0096 | 0.0099 | 0.0059 | 0.0144 | 0.0058 |
Vacuole Periphery | 0.0051 | 0.0034 | 0.005 | 0.0069 | 0.002 | 0.0049 | 0.0068 | 0.0022 | 0.004 | 0.0048 | 0.0051 | 0.003 | 0.0066 | 0.0033 | 0.0034 | 0.0039 | 0.0071 | 0.003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.8518 | 7.0455 | 11.3275 | 9.205 | 4.2578 | 14.4413 | 10.6856 | 14.0235 | 10.8756 | 10.685 |
Translational Efficiency | 1.4782 | 1.6452 | 1.1258 | 1.2653 | 2.3081 | 1.0533 | 1.4758 | 1.1233 | 1.5215 | 1.1566 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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