Standard name
Human Ortholog
Description ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.96 0.93 0.95 0.93 0.91 0.84 0.82 0.68 0.66 0.55 0.66 0.97 0.98 0.98 0.86 0.86 0.95 0.9 0.9 0.86 0.6 0.64 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0.05 0 0 0.17 0.14 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.08 0.14 0.25 0.47 0.41 0.56 0.28 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0.13 0.12 0.05 0 0 0 0.1 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 1 1 2 5 3 6 2 0 0 0 1 0 0 2 0 0 2 2
Bud 0 1 0 1 0 2 6 10 10 5 3 2 0 0 0 0 0 0 6 5 1 5 12
Bud Neck 0 0 1 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 2 3 0 2 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Cell Periphery 3 20 12 7 8 17 8 12 21 14 16 2 2 5 14 15 5 0 0 1 0 0 1
Cytoplasm 130 564 382 281 327 390 450 380 374 225 241 250 299 345 318 250 251 129 599 368 145 174 218
Endoplasmic Reticulum 2 4 7 12 4 9 5 10 10 6 33 3 3 0 11 13 9 7 15 10 40 37 45
Endosome 0 9 1 0 2 7 2 4 5 1 0 0 2 3 3 4 0 0 3 1 3 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 1 1 5 1 1 0 1 0 0 4 3
Mitochondria 2 8 0 6 29 66 137 260 234 229 101 3 1 3 7 2 4 0 3 17 18 13 22
Nucleus 3 2 2 1 1 0 2 0 3 1 1 0 0 2 5 4 1 0 4 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 3 1 2 0 0 0 0 0 0 0 1 2 0 1 0
Vac/Vac Membrane 0 39 9 5 18 15 7 12 31 18 10 0 1 1 49 36 12 1 21 10 23 23 31
Unique Cell Count 135 609 400 301 359 463 550 557 567 406 367 258 306 351 370 290 265 144 670 429 240 274 354
Labelled Cell Count 140 648 415 313 389 507 621 692 698 503 415 264 309 360 412 326 283 144 670 429 240 274 354


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.9 8.0 7.5 7.1 5.8 5.7 5.1 5.2 4.8 5.6 7.0 7.0 7.0 10.9 11.7 10.4 6.9 7.0 7.3
Std Deviation (1e-4) 1.7 1.7 1.4 1.4 1.3 1.2 1.1 0.9 1.0 1.0 1.0 1.4 1.5 1.5 2.0 1.7 1.8 1.5 1.8 2.1
Intensity Change (Log2) -0.09 -0.17 -0.45 -0.48 -0.63 -0.61 -0.74 -0.52 -0.18 -0.19 -0.19 0.46 0.55 0.38 -0.22 -0.19 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -0.7 0.5 -1.6 -0.8 0.6 0 1.0 -1.9 -2.2 -1.4 0.6 1.5 -0.9
Cytoplasm -1.2 -2.4 -5.4 -6.3 -10.3 -11.0 -13.2 -10.6 0.9 1.6 2.2 -4.6 -4.3 -0.5
Endoplasmic Reticulum 1.8 -0.7 0.2 -1.1 0.1 0 0 4.5 0 0 0 1.1 2.1 1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 5.8 7.9 10.8 16.0 14.8 0 11.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 2.1 0.9 -1.2 -0.1 2.5 1.7 0.4 0 0 0 5.8 5.3 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8405 4.382 3.182 3.2539 3.0138 3.7367 4.3358 5.3682 4.6436 3.8821 4.5955 4.5952 4.0438 4.9041 4.5062 4.1441 4.3184 4.587
Actin 0.0015 0.0028 0.0079 0.0011 0.0051 0.0023 0.0319 0.0029 0.0178 0.0024 0.0284 0.0058 0.0158 0.0006 0.0133 0.0004 0.0067 0.0071
Bud 0.0016 0.0019 0.0023 0.001 0.0006 0.0015 0.0017 0.0045 0.0035 0.0012 0.0092 0.0008 0.0067 0.002 0.0057 0.0006 0.0041 0.0036
Bud Neck 0.0008 0.0005 0.0008 0.0013 0.0012 0.0033 0.0045 0.0004 0.0012 0.0017 0.0031 0.0022 0.0047 0.0003 0.0011 0.0011 0.0022 0.0013
Bud Periphery 0.0008 0.0019 0.0035 0.0005 0.0006 0.002 0.0016 0.0052 0.0041 0.0008 0.0087 0.0007 0.002 0.0055 0.002 0.0003 0.0046 0.0016
Bud Site 0.0027 0.0109 0.0025 0.0004 0.0007 0.0017 0.0084 0.0072 0.0101 0.0007 0.0479 0.0012 0.0065 0.0024 0.0059 0.0012 0.0275 0.0002
Cell Periphery 0.0005 0.0006 0.0005 0.0001 0.0002 0.0002 0.0005 0.0004 0.0005 0.0002 0.001 0.0002 0.0004 0.0004 0.0004 0.0002 0.0004 0.0002
Cytoplasm 0.7054 0.823 0.7218 0.6696 0.7014 0.7399 0.5261 0.861 0.7454 0.7655 0.5263 0.7979 0.6102 0.9005 0.7878 0.7477 0.6646 0.8205
Cytoplasmic Foci 0.0384 0.0292 0.0403 0.0551 0.0373 0.0505 0.0549 0.0227 0.0302 0.0304 0.0641 0.0305 0.0612 0.0192 0.0268 0.0479 0.0449 0.0332
Eisosomes 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0014 0 0.0001 0 0.0006 0 0.0001 0
Endoplasmic Reticulum 0.0273 0.0103 0.0118 0.0184 0.0221 0.0201 0.0302 0.0139 0.015 0.0147 0.0068 0.0211 0.0288 0.0115 0.0169 0.009 0.0129 0.0142
Endosome 0.0934 0.0618 0.1144 0.1313 0.1061 0.1179 0.1499 0.0404 0.0778 0.0854 0.1231 0.0843 0.1208 0.0318 0.0748 0.1341 0.0756 0.0672
Golgi 0.0102 0.0129 0.0064 0.0147 0.0094 0.0114 0.0319 0.0058 0.0196 0.0106 0.0531 0.0113 0.0152 0.0031 0.0054 0.0045 0.0107 0.0067
Lipid Particles 0.0023 0.0002 0.0007 0.0005 0.0118 0.0004 0.0152 0.0004 0.0081 0.0009 0.0118 0.002 0.0094 0.0001 0.0027 0.0007 0.0042 0.0014
Mitochondria 0.0099 0.0144 0.0073 0.0051 0.006 0.009 0.0242 0.0019 0.0197 0.0023 0.0656 0.0078 0.0069 0.0021 0.005 0.0021 0.0314 0.0053
None 0.06 0.0132 0.0501 0.0718 0.0475 0.0228 0.0294 0.0166 0.0129 0.0467 0.0046 0.0127 0.0548 0.01 0.0212 0.0235 0.0877 0.0223
Nuclear Periphery 0.0054 0.0011 0.0018 0.0028 0.0201 0.0019 0.021 0.0015 0.0027 0.0063 0.0059 0.0035 0.0087 0.001 0.0037 0.0022 0.002 0.0029
Nucleolus 0.0018 0.0001 0.0002 0.0001 0.001 0.0001 0.0038 0.0001 0.0002 0.0001 0.0017 0.0003 0.0007 0 0.0015 0.0001 0.0003 0.0001
Nucleus 0.0083 0.0015 0.0023 0.0019 0.0043 0.0018 0.0257 0.0019 0.0018 0.0031 0.0058 0.0044 0.016 0.0013 0.0056 0.0021 0.002 0.0016
Peroxisomes 0.0005 0.0002 0.0003 0.0005 0.0011 0.0007 0.0059 0.0016 0.0101 0.0004 0.01 0.002 0.0084 0.0001 0.0014 0.0004 0.0081 0.0035
Punctate Nuclear 0.0051 0.0002 0.0008 0.0004 0.005 0.0011 0.0129 0.0005 0.0009 0.0006 0.0036 0.0016 0.0032 0.0002 0.0036 0.0009 0.0019 0.0007
Vacuole 0.0207 0.0106 0.0197 0.0178 0.0133 0.0084 0.015 0.0097 0.0153 0.0219 0.0133 0.007 0.0165 0.0064 0.0121 0.0171 0.0064 0.0047
Vacuole Periphery 0.0031 0.0026 0.0043 0.0055 0.005 0.003 0.0049 0.0015 0.003 0.0042 0.0043 0.0027 0.0031 0.0014 0.0026 0.0038 0.0019 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.1981 24.8383 30.6144 23.2702 24.1183 12.2376 23.6242 24.5635 21.145 25.1125
Translational Efficiency 1.1944 1.1935 0.9273 1.0556 1.0724 1.8076 1.1826 0.8048 0.8409 0.9072

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2268 1151 177 1431 1896 803 1677 95 4164 1954 1854 1526

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 711.76 869.57 1075.30 995.04 804.10 861.78 954.65 1193.44 753.81 866.37 966.17 1007.39
Standard Deviation 114.80 164.00 119.35 154.69 119.54 149.87 161.84 1327.76 125.69 158.40 162.20 366.73
Intensity Change Log 2 0.288911 0.595276 0.483364 0.099945 0.247597 0.569679 0.191761 0.421308 0.529793

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000185 0.000394 0.000420 0.000607 0.000223 0.001158 0.000142 0.000358 0.000203 0.000708 0.000169 0.000591
Bud Neck 0.011302 0.004599 0.002767 0.003310 0.006057 0.012316 0.003515 0.002209 0.008914 0.007770 0.003443 0.003241
Bud Site 0.021141 0.027776 0.010212 0.031735 0.022650 0.029099 0.008508 0.028195 0.021829 0.028320 0.008671 0.031514
Cell Periphery 0.000285 0.000155 0.000163 0.000136 0.000391 0.000315 0.000481 0.000155 0.000334 0.000221 0.000450 0.000137
Cytoplasm 0.578195* 0.558695* 0.362498* 0.685625* 0.513277* 0.410450* 0.575218* 0.375030* 0.548636* 0.497773* 0.554910* 0.666289*
Cytoplasmic Foci 0.224191* 0.242822* 0.126173 0.093997 0.268972* 0.302802* 0.093697 0.142499 0.244581* 0.267471* 0.096797 0.097016
Eisosomes 0.000116 0.000043 0.000081 0.000016 0.000091 0.000221 0.000154 0.000050 0.000104 0.000116 0.000147 0.000018
Endoplasmic Reticulum 0.008362 0.004118 0.003750 0.001075 0.011939 0.004105 0.002445 0.005387 0.009991 0.004112 0.002570 0.001343
Endosome 0.014535 0.026896 0.037015 0.010935 0.025951 0.051954 0.014426 0.034602 0.019733 0.037194 0.016583 0.012408
Golgi 0.016295 0.011957 0.000699 0.003020 0.021837 0.025120 0.001659 0.001066 0.018818 0.017366 0.001567 0.002898
Lipid Particles 0.006351 0.004369 0.007491 0.003016 0.005170 0.009916 0.004336 0.004818 0.005814 0.006649 0.004637 0.003128
Mitochondria 0.012662 0.004766 0.000113 0.001832 0.007859 0.010169 0.000333 0.000080 0.010475 0.006987 0.000312 0.001723
Mitotic Spindle 0.000857 0.002441 0.003925 0.011968 0.000667 0.004240 0.003116 0.000576 0.000771 0.003180 0.003193 0.011258
None 0.009991 0.002040 0.000553 0.000577 0.002005 0.000687 0.005766 0.000635 0.006355 0.001484 0.005268 0.000581
Nuclear Periphery 0.000187 0.000245 0.002312 0.000181 0.000137 0.000253 0.000689 0.000584 0.000165 0.000249 0.000844 0.000206
Nuclear Periphery Foci 0.000322 0.000570 0.008797 0.001358 0.000458 0.000621 0.002215 0.004588 0.000384 0.000591 0.002843 0.001559
Nucleolus 0.000663 0.000365 0.000539 0.000233 0.000244 0.000589 0.000533 0.000256 0.000472 0.000457 0.000533 0.000234
Nucleus 0.032186 0.018390 0.046548 0.022200 0.014615 0.021392 0.114051 0.050052 0.024186 0.019624 0.107607 0.023934
Peroxisomes 0.001949 0.002384 0.001074 0.002105 0.001542 0.004859 0.000792 0.001588 0.001764 0.003401 0.000819 0.002073
Vacuole 0.048845 0.074852 0.383245* 0.123443 0.078123 0.099944 0.166524 0.345198* 0.062176 0.085163 0.187214 0.137248
Vacuole Periphery 0.011379 0.012122 0.001627 0.002634 0.017788 0.009790 0.001400 0.002075 0.014297 0.011164 0.001421 0.002599

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.13 -3.83 -4.56 -1.49 -0.91 -2.11 3.19 -0.76 1.76 -1.19 -2.70 0.98 -4.45 0.91 -4.63
Bud Neck 7.76 10.41 10.02 2.37 -1.28 -5.10 4.88 6.41 8.10 1.92 1.65 9.91 11.07 7.45 -0.03
Bud Site -3.29 7.18 -1.83 0.95 -6.54 -2.89 10.77 -0.31 0.43 -2.03 -4.34 12.58 -1.65 1.31 -8.09
Cell Periphery 6.79 5.91 9.45 2.86 3.02 0.77 -0.19 0.98 0.57 1.34 2.45 -0.83 4.72 5.40 5.33
Cytoplasm 2.02 10.62 -5.40 -6.17 -12.54 8.88 -6.03 4.26 0.85 6.07 6.61 0.04 -7.56 -11.57 -6.39
Cytoplasmic Foci -2.51 11.92 28.03 23.39 3.69 -3.66 32.29 9.32 10.70 -3.27 -3.90 36.87 36.48 31.67 2.09
Eisosomes 8.73 2.93 13.85 6.30 6.55 -3.34 -2.26 4.05 4.29 4.13 -0.73 -1.40 18.29 6.03 6.69
Endoplasmic Reticulum 3.44 3.80 7.39 4.04 4.62 5.92 8.26 3.72 -0.24 -1.37 6.47 9.53 11.50 5.23 3.54
Endosome -8.01 -5.64 6.78 11.83 7.51 -9.61 10.20 -0.58 3.31 -2.59 -11.86 4.88 10.83 17.83 5.06
Golgi 4.32 23.42 20.02 12.13 -4.96 -1.36 20.98 22.89 10.92 0.58 1.36 25.64 28.78 14.77 -1.14
Lipid Particles 2.59 -0.49 7.54 2.25 3.89 -3.33 2.89 0.41 3.05 -0.79 -1.23 3.93 8.46 5.02 3.11
Mitochondria 5.72 14.54 10.91 2.84 -1.81 -1.31 9.78 10.08 6.51 5.77 3.27 17.33 11.48 5.46 -1.54
Mitotic Spindle -1.88 -1.78 -6.78 -5.35 -1.23 -2.72 -4.54 0.16 2.74 4.55 -3.30 -4.80 -6.85 -4.83 -3.77
None 6.74 8.89 8.83 2.39 -0.69 2.37 -2.68 2.26 0.30 4.25 6.93 1.68 9.02 2.30 4.35
Nuclear Periphery -0.84 -2.11 -5.18 -2.48 1.85 -1.85 -6.73 -3.35 -2.32 0.74 -1.75 -5.85 -6.48 -3.27 3.33
Nuclear Periphery Foci -3.80 -8.84 -10.84 -8.68 7.17 -0.70 -6.69 -3.37 -3.22 -1.89 -1.73 -11.94 -9.34 -8.41 3.41
Nucleolus 4.22 0.86 6.83 1.87 2.23 -2.12 -4.01 0.06 2.06 3.26 0.05 -0.48 5.55 2.46 3.73
Nucleus 5.37 -2.05 -1.25 -5.61 1.73 -2.22 -18.60 -3.38 -2.62 4.79 2.16 -16.92 -5.41 -6.05 13.25
Peroxisomes -1.79 6.72 1.56 2.57 -2.78 -7.58 7.53 0.29 5.96 -1.73 -7.02 10.63 0.80 6.20 -4.29
Vacuole -6.70 -16.64 -23.23 -16.86 9.59 -3.77 -18.63 -9.05 -8.22 -5.44 -6.93 -26.22 -22.86 -17.48 0.54
Vacuole Periphery -0.49 13.80 12.40 8.33 -1.02 7.41 18.93 16.58 9.73 -1.06 3.43 22.64 19.85 11.41 -1.30