Standard name | |
---|---|
Description | ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 1 | 1 | – | 2 | 5 | 3 | 6 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 2 | 2 |
Bud | 0 | 1 | 0 | 1 | 0 | 2 | 6 | – | 10 | 10 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 1 | 5 | 12 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 20 | 12 | 7 | 8 | 17 | 8 | – | 12 | 21 | 14 | 16 | 2 | 2 | 5 | 14 | 15 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 130 | 564 | 382 | 281 | 327 | 390 | 450 | – | 380 | 374 | 225 | 241 | 250 | 299 | 345 | 318 | 250 | 251 | 129 | 599 | 368 | 145 | 174 | 218 |
Endoplasmic Reticulum | 2 | 4 | 7 | 12 | 4 | 9 | 5 | – | 10 | 10 | 6 | 33 | 3 | 3 | 0 | 11 | 13 | 9 | 7 | 15 | 10 | 40 | 37 | 45 |
Endosome | 0 | 9 | 1 | 0 | 2 | 7 | 2 | – | 4 | 5 | 1 | 0 | 0 | 2 | 3 | 3 | 4 | 0 | 0 | 3 | 1 | 3 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 4 | 3 |
Mitochondria | 2 | 8 | 0 | 6 | 29 | 66 | 137 | – | 260 | 234 | 229 | 101 | 3 | 1 | 3 | 7 | 2 | 4 | 0 | 3 | 17 | 18 | 13 | 22 |
Nucleus | 3 | 2 | 2 | 1 | 1 | 0 | 2 | – | 0 | 3 | 1 | 1 | 0 | 0 | 2 | 5 | 4 | 1 | 0 | 4 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 39 | 9 | 5 | 18 | 15 | 7 | – | 12 | 31 | 18 | 10 | 0 | 1 | 1 | 49 | 36 | 12 | 1 | 21 | 10 | 23 | 23 | 31 |
Unique Cell Count | 135 | 609 | 400 | 301 | 359 | 463 | 550 | 557 | 567 | 406 | 367 | 258 | 306 | 351 | 370 | 290 | 265 | 144 | 670 | 429 | 240 | 274 | 354 | |
Labelled Cell Count | 140 | 648 | 415 | 313 | 389 | 507 | 621 | 692 | 698 | 503 | 415 | 264 | 309 | 360 | 412 | 326 | 283 | 144 | 670 | 429 | 240 | 274 | 354 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 8.9 | 8.0 | 7.5 | 7.1 | 5.8 | 5.7 | – | 5.1 | 5.2 | 4.8 | 5.6 | 7.0 | 7.0 | 7.0 | 10.9 | 11.7 | 10.4 | 6.9 | 7.0 | 7.3 |
Std Deviation (1e-4) | 1.7 | 1.7 | 1.4 | 1.4 | 1.3 | 1.2 | 1.1 | – | 0.9 | 1.0 | 1.0 | 1.0 | 1.4 | 1.5 | 1.5 | 2.0 | 1.7 | 1.8 | 1.5 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.17 | -0.45 | -0.48 | – | -0.63 | -0.61 | -0.74 | -0.52 | -0.18 | -0.19 | -0.19 | 0.46 | 0.55 | 0.38 | -0.22 | -0.19 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | -0.7 | 0.5 | -1.6 | – | -0.8 | 0.6 | 0 | 1.0 | -1.9 | -2.2 | -1.4 | 0.6 | 1.5 | -0.9 |
Cytoplasm | -1.2 | -2.4 | -5.4 | -6.3 | – | -10.3 | -11.0 | -13.2 | -10.6 | 0.9 | 1.6 | 2.2 | -4.6 | -4.3 | -0.5 |
Endoplasmic Reticulum | 1.8 | -0.7 | 0.2 | -1.1 | – | 0.1 | 0 | 0 | 4.5 | 0 | 0 | 0 | 1.1 | 2.1 | 1.4 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 5.8 | 7.9 | 10.8 | – | 16.0 | 14.8 | 0 | 11.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | 2.1 | 0.9 | -1.2 | – | -0.1 | 2.5 | 1.7 | 0.4 | 0 | 0 | 0 | 5.8 | 5.3 | 1.6 |