Standard name
Description ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.96 0.93 0.95 0.93 0.91 0.84 0.82 – 0.68 0.66 0.55 0.66 0.97 0.98 0.98 0.86 0.86 0.95 0.9 0.9 0.86 0.6 0.64 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.09 0 0 0 0 0 0 0.05 0 0 0.17 0.14 0.13
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.08 0.14 0.25 – 0.47 0.41 0.56 0.28 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0.05 0 0 – 0 0.05 0 0 0 0 0 0.13 0.12 0.05 0 0 0 0.1 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 1 1 – 2 5 3 6 2 0 0 0 1 0 0 2 0 0 2 2
Bud 0 1 0 1 0 2 6 – 10 10 5 3 2 0 0 0 0 0 0 6 5 1 5 12
Bud Neck 0 0 1 0 0 0 1 – 0 1 0 1 0 0 0 0 0 0 0 2 3 0 2 4
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 – – – – – –
Cell Periphery 3 20 12 7 8 17 8 – 12 21 14 16 2 2 5 14 15 5 0 0 1 0 0 1
Cytoplasm 130 564 382 281 327 390 450 – 380 374 225 241 250 299 345 318 250 251 129 599 368 145 174 218
Endoplasmic Reticulum 2 4 7 12 4 9 5 – 10 10 6 33 3 3 0 11 13 9 7 15 10 40 37 45
Endosome 0 9 1 0 2 7 2 – 4 5 1 0 0 2 3 3 4 0 0 3 1 3 1 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 1 1 5 1 1 0 1 0 0 4 3
Mitochondria 2 8 0 6 29 66 137 – 260 234 229 101 3 1 3 7 2 4 0 3 17 18 13 22
Nucleus 3 2 2 1 1 0 2 – 0 3 1 1 0 0 2 5 4 1 0 4 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 2 3 1 2 0 0 0 0 0 0 0 1 2 0 1 0
Vac/Vac Membrane 0 39 9 5 18 15 7 – 12 31 18 10 0 1 1 49 36 12 1 21 10 23 23 31
Unique Cell Count 135 609 400 301 359 463 550 557 567 406 367 258 306 351 370 290 265 144 670 429 240 274 354
Labelled Cell Count 140 648 415 313 389 507 621 692 698 503 415 264 309 360 412 326 283 144 670 429 240 274 354


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.9 8.0 7.5 7.1 5.8 5.7 – 5.1 5.2 4.8 5.6 7.0 7.0 7.0 10.9 11.7 10.4 6.9 7.0 7.3
Std Deviation (1e-4) 1.7 1.7 1.4 1.4 1.3 1.2 1.1 – 0.9 1.0 1.0 1.0 1.4 1.5 1.5 2.0 1.7 1.8 1.5 1.8 2.1
Intensity Change (Log2) – – – -0.09 -0.17 -0.45 -0.48 – -0.63 -0.61 -0.74 -0.52 -0.18 -0.19 -0.19 0.46 0.55 0.38 -0.22 -0.19 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -0.7 0.5 -1.6 – -0.8 0.6 0 1.0 -1.9 -2.2 -1.4 0.6 1.5 -0.9
Cytoplasm -1.2 -2.4 -5.4 -6.3 – -10.3 -11.0 -13.2 -10.6 0.9 1.6 2.2 -4.6 -4.3 -0.5
Endoplasmic Reticulum 1.8 -0.7 0.2 -1.1 – 0.1 0 0 4.5 0 0 0 1.1 2.1 1.4
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 5.8 7.9 10.8 – 16.0 14.8 0 11.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 2.1 0.9 -1.2 – -0.1 2.5 1.7 0.4 0 0 0 5.8 5.3 1.6