Standard name | |
---|---|
Human Ortholog | |
Description | Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 2 | 0 | 3 | 7 | 3 | 4 | 2 | – | 4 | 5 | 3 | 2 | 1 | 3 | 1 | 3 | 0 | 0 | 2 | 0 | 0 | 1 |
Bud | 0 | 2 | 6 | 1 | 2 | 5 | 5 | 5 | 6 | – | 7 | 8 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 26 | 6 | 10 | 3 | 14 | 8 | 16 | 6 | 14 | – | 16 | 28 | 1 | 12 | 6 | 5 | 7 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 458 | 370 | 331 | 279 | 286 | 369 | 346 | 375 | 251 | – | 243 | 296 | 188 | 245 | 273 | 274 | 168 | 200 | 519 | 369 | 331 | 151 | 180 | 172 |
Endoplasmic Reticulum | 18 | 14 | 12 | 9 | 13 | 14 | 19 | 20 | 15 | – | 24 | 27 | 5 | 3 | 2 | 16 | 9 | 9 | 12 | 7 | 15 | 12 | 12 | 31 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | – | 0 | 0 | 3 | 1 | 0 | 5 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 2 |
Golgi | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 2 | 0 | 0 | 2 | 0 | 1 | 1 |
Mitochondria | 5 | 0 | 9 | 35 | 49 | 117 | 161 | 176 | 199 | – | 239 | 307 | 3 | 4 | 27 | 6 | 1 | 3 | 8 | 1 | 9 | 2 | 1 | 1 |
Nucleus | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | – | 0 | 3 | 0 | 2 | 0 | 5 | 3 | 3 | 2 | 4 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 17 | 4 | 6 | 0 | 8 | 5 | 8 | 13 | 14 | – | 9 | 16 | 6 | 2 | 0 | 32 | 25 | 34 | 17 | 10 | 10 | 3 | 5 | 5 |
Unique Cell Count | 504 | 385 | 355 | 297 | 336 | 451 | 481 | 532 | 412 | 430 | 546 | 202 | 254 | 281 | 326 | 195 | 237 | 569 | 399 | 382 | 174 | 208 | 224 | |
Labelled Cell Count | 532 | 402 | 378 | 327 | 375 | 525 | 563 | 602 | 502 | 542 | 691 | 210 | 271 | 315 | 350 | 220 | 267 | 569 | 399 | 382 | 174 | 208 | 224 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.6 | 11.6 | 8.4 | 7.7 | 8.0 | 7.2 | 6.9 | 6.4 | 6.1 | – | 5.8 | 5.6 | 9.9 | 9.7 | 8.9 | 11.5 | 12.9 | 12.1 | 10.9 | 11.9 | 12.3 |
Std Deviation (1e-4) | 4.5 | 1.9 | 1.3 | 1.1 | 1.4 | 1.1 | 1.3 | 1.1 | 1.3 | – | 1.2 | 1.1 | 1.5 | 1.5 | 1.4 | 2.8 | 3.0 | 3.2 | 1.8 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.07 | -0.21 | -0.28 | -0.39 | -0.46 | – | -0.52 | -0.59 | 0.24 | 0.2 | 0.08 | 0.46 | 0.63 | 0.53 | 0.38 | 0.51 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | -0.3 | – | -0.1 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.6 | 1.0 | -1.0 | 0.4 | -1.9 | 0.5 | – | 0.7 | 1.7 | 0 | 1.2 | -0.5 | -1.1 | 0.5 | 1.2 |
Cytoplasm | 0.4 | -3.5 | -4.8 | -7.8 | -8.2 | -10.4 | – | -11.6 | -12.4 | -0.1 | 1.7 | 2.2 | -3.8 | -2.7 | -3.5 |
Endoplasmic Reticulum | -0.3 | 0.3 | -0.2 | 0.4 | 0.3 | 0.2 | – | 1.5 | 1.1 | -0.6 | -1.7 | -2.3 | 1.0 | 0.7 | 0.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.7 | 5.7 | 9.1 | 11.0 | 11.0 | 14.2 | – | 15.9 | 16.5 | 0 | -0.8 | 3.8 | -0.6 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0.6 | 0 | 0 | 0.8 | 1.5 | – | 0.4 | 1.2 | 0 | 0 | 0 | 4.6 | 5.4 | 6.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.7253 | 13.9374 | 12.5071 | 11.1939 | 11.6014 | 12.3356 | 9.5185 | 12.4463 | 11.1359 | 10.5066 | 10.0554 | 11.2516 | 11.1996 | 12.6508 | 11.9623 | 11.577 | 11.0361 | 12.0383 |
Actin | 0.001 | 0.001 | 0.0009 | 0.0023 | 0.0011 | 0.001 | 0.0021 | 0.0002 | 0.0016 | 0.0003 | 0.0001 | 0.0004 | 0.0013 | 0.0024 | 0.001 | 0.0009 | 0.0002 | 0.0009 |
Bud | 0.0011 | 0.0007 | 0.0013 | 0.0022 | 0.0006 | 0.0008 | 0.0004 | 0.0004 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0007 | 0.0006 | 0.0005 | 0.0004 | 0.0005 |
Bud Neck | 0.0011 | 0.0008 | 0.0012 | 0.0014 | 0.0016 | 0.0033 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0004 | 0.0011 | 0.0004 | 0.0004 | 0.0005 | 0.0006 | 0.0006 | 0.0012 |
Bud Periphery | 0.0022 | 0.0008 | 0.0015 | 0.0048 | 0.0005 | 0.0017 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0005 |
Bud Site | 0.0009 | 0.0014 | 0.0016 | 0.0011 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0018 | 0.0006 | 0.0003 | 0.0002 | 0.0002 |
Cell Periphery | 0.0035 | 0.002 | 0.0018 | 0.0042 | 0.0005 | 0.0024 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0003 |
Cytoplasm | 0.7853 | 0.9037 | 0.8294 | 0.7871 | 0.8287 | 0.8349 | 0.8521 | 0.9576 | 0.9025 | 0.8631 | 0.8714 | 0.8865 | 0.8434 | 0.9118 | 0.8834 | 0.8079 | 0.9048 | 0.8635 |
Cytoplasmic Foci | 0.008 | 0.0064 | 0.0072 | 0.013 | 0.0117 | 0.0102 | 0.0204 | 0.0086 | 0.0173 | 0.0218 | 0.0163 | 0.0184 | 0.0172 | 0.0178 | 0.0152 | 0.0168 | 0.0199 | 0.0215 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0982 | 0.0435 | 0.0724 | 0.0321 | 0.0832 | 0.0846 | 0.0434 | 0.0069 | 0.0099 | 0.0076 | 0.0188 | 0.0272 | 0.0521 | 0.0161 | 0.0193 | 0.0347 | 0.0034 | 0.0267 |
Endosome | 0.0324 | 0.0165 | 0.0313 | 0.0669 | 0.0398 | 0.0274 | 0.0478 | 0.0153 | 0.044 | 0.08 | 0.0697 | 0.0473 | 0.0543 | 0.0301 | 0.0523 | 0.0822 | 0.0576 | 0.0549 |
Golgi | 0.0065 | 0.0032 | 0.0036 | 0.0085 | 0.0061 | 0.0048 | 0.008 | 0.0022 | 0.0043 | 0.0036 | 0.0034 | 0.0073 | 0.0093 | 0.0079 | 0.005 | 0.0106 | 0.0033 | 0.0127 |
Lipid Particles | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0034 | 0 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 |
Mitochondria | 0.0136 | 0.0029 | 0.0047 | 0.0044 | 0.0036 | 0.012 | 0.0058 | 0.0028 | 0.0024 | 0.0021 | 0.0023 | 0.0051 | 0.0051 | 0.0025 | 0.0033 | 0.0037 | 0.0014 | 0.0099 |
None | 0.0087 | 0.0007 | 0.0007 | 0.0007 | 0.0007 | 0.0008 | 0.003 | 0.0008 | 0.0014 | 0.0012 | 0.0015 | 0.001 | 0.0017 | 0.0009 | 0.0007 | 0.0006 | 0.0005 | 0.0008 |
Nuclear Periphery | 0.0041 | 0.0015 | 0.0045 | 0.0043 | 0.0034 | 0.002 | 0.0021 | 0.0004 | 0.0009 | 0.0012 | 0.002 | 0.0006 | 0.0016 | 0.0008 | 0.0014 | 0.0017 | 0.0005 | 0.0007 |
Nucleolus | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.002 | 0.0013 | 0.0021 | 0.0024 | 0.0015 | 0.0013 | 0.001 | 0.0005 | 0.001 | 0.001 | 0.0012 | 0.0007 | 0.0008 | 0.0008 | 0.0009 | 0.0008 | 0.0007 | 0.0007 |
Peroxisomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0019 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.0255 | 0.0115 | 0.0295 | 0.0516 | 0.013 | 0.0089 | 0.0058 | 0.0025 | 0.0081 | 0.0115 | 0.0082 | 0.0025 | 0.0078 | 0.0042 | 0.0114 | 0.0335 | 0.0045 | 0.0032 |
Vacuole Periphery | 0.0047 | 0.0019 | 0.0057 | 0.0127 | 0.0031 | 0.0032 | 0.0016 | 0.0006 | 0.0019 | 0.0044 | 0.0038 | 0.0012 | 0.0021 | 0.001 | 0.0033 | 0.004 | 0.0018 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.1271 | 27.3142 | 33.9069 | 37.482 | 25.3615 | 25.1154 | 31.8762 | 34.4054 | 39.1013 | 34.6752 |
Translational Efficiency | 1.5402 | 1.3103 | 1.1063 | 1.2249 | 1.2374 | 1.4417 | 1.087 | 0.8938 | 0.8978 | 0.9892 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2186 | 671 | 279 | 988 | 1750 | 2089 | 129 | 870 | 3936 | 2760 | 408 | 1858 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 920.35 | 1205.68 | 1481.50 | 1428.77 | 962.66 | 1099.81 | 1443.23 | 1437.97 | 939.16 | 1125.55 | 1469.40 | 1433.08 |
Standard Deviation | 123.03 | 185.52 | 180.70 | 212.49 | 183.00 | 163.12 | 158.24 | 233.29 | 154.07 | 174.84 | 174.82 | 222.52 |
Intensity Change Log 2 | — | 0.389593 | 0.686804 | 0.634519 | — | 0.192156 | 0.584203 | 0.578935 | — | 0.292033 | 0.635263 | 0.606370 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000230 | 0.000432 | 0.000451 | 0.000917 | 0.000191 | 0.000284 | 0.001170 | 0.000551 | 0.000212 | 0.000320 | 0.000678 | 0.000746 |
Bud Neck | 0.001207 | 0.000506 | 0.002473 | 0.001603 | 0.000875 | 0.000730 | 0.002140 | 0.001309 | 0.001059 | 0.000675 | 0.002368 | 0.001465 |
Bud Site | 0.034697 | 0.044553 | 0.020147 | 0.036882 | 0.025084 | 0.029220 | 0.038059 | 0.036567 | 0.030423 | 0.032948 | 0.025811 | 0.036734 |
Cell Periphery | 0.000384 | 0.000423 | 0.000215 | 0.001730 | 0.000237 | 0.000180 | 0.000440 | 0.001865 | 0.000319 | 0.000239 | 0.000286 | 0.001793 |
Cytoplasm | 0.616151* | 0.608408* | 0.469681* | 0.828863* | 0.592328* | 0.637450* | 0.630707* | 0.834191* | 0.605559* | 0.630389* | 0.520594* | 0.831358* |
Cytoplasmic Foci | 0.223705* | 0.252706* | 0.105883 | 0.039297 | 0.241362* | 0.211932* | 0.145525 | 0.043161 | 0.231555* | 0.221845* | 0.118417 | 0.041107 |
Eisosomes | 0.000026 | 0.000009 | 0.000010 | 0.000007 | 0.000014 | 0.000011 | 0.000014 | 0.000005 | 0.000020 | 0.000010 | 0.000011 | 0.000006 |
Endoplasmic Reticulum | 0.009954 | 0.005013 | 0.005377 | 0.000737 | 0.009462 | 0.003782 | 0.003588 | 0.000637 | 0.009735 | 0.004081 | 0.004811 | 0.000690 |
Endosome | 0.009966 | 0.012490 | 0.035667 | 0.005629 | 0.015984 | 0.014798 | 0.022171 | 0.003974 | 0.012642 | 0.014237 | 0.031400 | 0.004854 |
Golgi | 0.023062 | 0.030369 | 0.006900 | 0.010755 | 0.029161 | 0.027219 | 0.007103 | 0.009694 | 0.025773 | 0.027985 | 0.006964 | 0.010258 |
Lipid Particles | 0.000872 | 0.000628 | 0.004649 | 0.005533 | 0.001253 | 0.000772 | 0.004065 | 0.007821 | 0.001041 | 0.000737 | 0.004464 | 0.006605 |
Mitochondria | 0.007081 | 0.000677 | 0.000054 | 0.000654 | 0.003054 | 0.002790 | 0.000021 | 0.002145 | 0.005290 | 0.002276 | 0.000044 | 0.001352 |
Mitotic Spindle | 0.000335 | 0.005957 | 0.006883 | 0.003272 | 0.000800 | 0.002401 | 0.004327 | 0.002356 | 0.000542 | 0.003266 | 0.006075 | 0.002843 |
None | 0.000832 | 0.000124 | 0.000068 | 0.000096 | 0.001358 | 0.000274 | 0.000047 | 0.000141 | 0.001066 | 0.000238 | 0.000061 | 0.000117 |
Nuclear Periphery | 0.000024 | 0.000018 | 0.000277 | 0.000029 | 0.000156 | 0.000035 | 0.000089 | 0.000027 | 0.000082 | 0.000031 | 0.000217 | 0.000028 |
Nuclear Periphery Foci | 0.000116 | 0.000334 | 0.005311 | 0.001143 | 0.000455 | 0.000198 | 0.003584 | 0.001253 | 0.000267 | 0.000231 | 0.004765 | 0.001194 |
Nucleolus | 0.000054 | 0.000061 | 0.000101 | 0.000077 | 0.000298 | 0.000384 | 0.000039 | 0.000105 | 0.000162 | 0.000305 | 0.000081 | 0.000090 |
Nucleus | 0.002056 | 0.001214 | 0.004672 | 0.002144 | 0.004111 | 0.001608 | 0.003854 | 0.002139 | 0.002970 | 0.001512 | 0.004413 | 0.002141 |
Peroxisomes | 0.000571 | 0.000570 | 0.000607 | 0.001130 | 0.000471 | 0.000651 | 0.001069 | 0.000862 | 0.000526 | 0.000631 | 0.000753 | 0.001004 |
Vacuole | 0.020812 | 0.014490 | 0.314385* | 0.054226 | 0.032469 | 0.036056 | 0.128708 | 0.046388 | 0.025995 | 0.030812 | 0.255678* | 0.050556 |
Vacuole Periphery | 0.047867 | 0.021019 | 0.016188 | 0.005277 | 0.040880 | 0.029229 | 0.003279 | 0.004810 | 0.044760 | 0.027233 | 0.012107 | 0.005058 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.63 | -3.36 | -3.22 | -2.12 | -2.00 | -2.45 | -3.77 | -7.32 | -4.73 | 2.48 | -2.97 | -4.93 | -4.63 | -3.60 | -0.10 |
Bud Neck | 7.31 | -6.21 | -3.67 | -9.91 | 3.63 | 1.53 | -4.22 | -4.01 | -5.69 | 2.60 | 5.43 | -7.81 | -5.26 | -10.20 | 4.81 |
Bud Site | -3.84 | 6.12 | -0.66 | 2.44 | -5.09 | -2.48 | -2.46 | -4.01 | -2.55 | 0.43 | -1.88 | 1.70 | -3.13 | -1.72 | -3.51 |
Cell Periphery | -0.85 | 5.85 | -2.94 | -2.84 | -3.34 | 3.65 | -1.91 | -7.43 | -7.71 | -5.82 | 4.61 | 0.90 | -5.65 | -5.98 | -5.74 |
Cytoplasm | 0.61 | 7.48 | -23.58 | -16.42 | -17.90 | -4.73 | -1.15 | -24.83 | -20.72 | -8.21 | -3.41 | 5.50 | -34.16 | -26.76 | -19.30 |
Cytoplasmic Foci | -3.11 | 15.30 | 40.61 | 24.81 | 9.48 | 4.40 | 7.39 | 35.90 | 32.76 | 8.80 | 1.93 | 16.87 | 54.03 | 42.00 | 12.66 |
Eisosomes | 10.39 | 8.86 | 11.31 | 1.59 | 2.33 | 3.08 | -0.68 | 9.97 | 7.61 | 5.45 | 10.35 | 6.94 | 14.37 | 5.94 | 5.16 |
Endoplasmic Reticulum | 3.63 | 3.79 | 9.31 | 4.47 | 5.38 | 4.48 | 4.18 | 7.50 | 6.59 | 3.19 | 6.53 | 5.29 | 11.93 | 7.82 | 6.28 |
Endosome | -2.65 | -8.86 | 6.09 | 6.50 | 10.29 | 1.06 | -1.65 | 12.75 | 14.70 | 5.52 | -2.34 | -8.12 | 13.56 | 14.57 | 11.74 |
Golgi | -4.33 | 16.40 | 7.50 | 9.08 | -1.95 | 1.38 | 15.49 | 15.40 | 12.48 | -1.80 | -2.14 | 23.05 | 14.61 | 13.88 | -2.69 |
Lipid Particles | 4.01 | -6.75 | -10.10 | -10.58 | -1.36 | 3.59 | -5.39 | -6.07 | -6.53 | -3.14 | 3.84 | -8.18 | -9.99 | -10.51 | -3.11 |
Mitochondria | 15.70 | 17.75 | 12.48 | 0.21 | -1.65 | 0.64 | 14.84 | 0.71 | 0.52 | -1.42 | 8.34 | 21.86 | 5.52 | 1.36 | -1.79 |
Mitotic Spindle | -2.68 | -3.51 | -2.28 | 1.19 | 1.66 | -2.76 | -3.11 | -1.68 | -0.64 | 0.32 | -4.08 | -4.16 | -2.77 | -0.01 | 1.60 |
None | 3.53 | 3.85 | 3.71 | 1.08 | -1.22 | 3.23 | 3.94 | 3.66 | 1.86 | -1.97 | 4.38 | 5.45 | 5.14 | 2.45 | -2.27 |
Nuclear Periphery | 0.91 | -5.79 | -0.77 | -1.28 | 5.57 | 1.98 | 0.99 | 2.10 | 0.43 | 3.02 | 1.81 | -3.39 | 1.93 | 0.18 | 6.04 |
Nuclear Periphery Foci | -3.26 | -8.51 | -13.52 | -8.46 | 6.65 | 2.34 | -6.58 | -6.67 | -9.78 | 4.83 | 0.22 | -10.09 | -13.74 | -13.22 | 7.85 |
Nucleolus | -0.29 | -3.60 | -4.27 | -0.27 | 1.78 | -0.66 | 4.24 | 3.00 | 1.47 | -2.44 | -1.10 | 2.69 | 2.45 | 1.50 | -0.36 |
Nucleus | 2.24 | -4.48 | -1.87 | -3.49 | 3.79 | 3.52 | -0.33 | 2.50 | -2.68 | 1.71 | 3.95 | -2.83 | 1.57 | -4.15 | 4.00 |
Peroxisomes | -0.11 | -0.60 | -5.11 | -4.13 | -4.24 | -3.02 | -2.31 | -4.43 | -2.13 | 0.88 | -2.31 | -2.45 | -6.71 | -4.49 | -1.91 |
Vacuole | 3.51 | -17.16 | -12.06 | -13.18 | 14.65 | -1.45 | -6.01 | -4.89 | -3.98 | 4.90 | -2.92 | -17.12 | -12.16 | -9.49 | 14.79 |
Vacuole Periphery | 9.78 | 11.45 | 19.36 | 7.89 | 4.82 | 4.82 | 18.10 | 17.69 | 15.56 | -1.63 | 9.48 | 16.66 | 26.13 | 17.18 | 4.41 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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