Standard name | |
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Description | Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 2 | 0 | 3 | 7 | 3 | 4 | 2 | – | 4 | 5 | 3 | 2 | 1 | 3 | 1 | 3 | 0 | 0 | 2 | 0 | 0 | 1 |
Bud | 0 | 2 | 6 | 1 | 2 | 5 | 5 | 5 | 6 | – | 7 | 8 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 26 | 6 | 10 | 3 | 14 | 8 | 16 | 6 | 14 | – | 16 | 28 | 1 | 12 | 6 | 5 | 7 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 458 | 370 | 331 | 279 | 286 | 369 | 346 | 375 | 251 | – | 243 | 296 | 188 | 245 | 273 | 274 | 168 | 200 | 519 | 369 | 331 | 151 | 180 | 172 |
Endoplasmic Reticulum | 18 | 14 | 12 | 9 | 13 | 14 | 19 | 20 | 15 | – | 24 | 27 | 5 | 3 | 2 | 16 | 9 | 9 | 12 | 7 | 15 | 12 | 12 | 31 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | – | 0 | 0 | 3 | 1 | 0 | 5 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 2 |
Golgi | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 2 | 0 | 0 | 2 | 0 | 1 | 1 |
Mitochondria | 5 | 0 | 9 | 35 | 49 | 117 | 161 | 176 | 199 | – | 239 | 307 | 3 | 4 | 27 | 6 | 1 | 3 | 8 | 1 | 9 | 2 | 1 | 1 |
Nucleus | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | – | 0 | 3 | 0 | 2 | 0 | 5 | 3 | 3 | 2 | 4 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 17 | 4 | 6 | 0 | 8 | 5 | 8 | 13 | 14 | – | 9 | 16 | 6 | 2 | 0 | 32 | 25 | 34 | 17 | 10 | 10 | 3 | 5 | 5 |
Unique Cell Count | 504 | 385 | 355 | 297 | 336 | 451 | 481 | 532 | 412 | 430 | 546 | 202 | 254 | 281 | 326 | 195 | 237 | 569 | 399 | 382 | 174 | 208 | 224 | |
Labelled Cell Count | 532 | 402 | 378 | 327 | 375 | 525 | 563 | 602 | 502 | 542 | 691 | 210 | 271 | 315 | 350 | 220 | 267 | 569 | 399 | 382 | 174 | 208 | 224 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 14.6 | 11.6 | 8.4 | 7.7 | 8.0 | 7.2 | 6.9 | 6.4 | 6.1 | – | 5.8 | 5.6 | 9.9 | 9.7 | 8.9 | 11.5 | 12.9 | 12.1 | 10.9 | 11.9 | 12.3 |
Std Deviation (1e-4) | 4.5 | 1.9 | 1.3 | 1.1 | 1.4 | 1.1 | 1.3 | 1.1 | 1.3 | – | 1.2 | 1.1 | 1.5 | 1.5 | 1.4 | 2.8 | 3.0 | 3.2 | 1.8 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.07 | -0.21 | -0.28 | -0.39 | -0.46 | – | -0.52 | -0.59 | 0.24 | 0.2 | 0.08 | 0.46 | 0.63 | 0.53 | 0.38 | 0.51 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | -0.3 | – | -0.1 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.6 | 1.0 | -1.0 | 0.4 | -1.9 | 0.5 | – | 0.7 | 1.7 | 0 | 1.2 | -0.5 | -1.1 | 0.5 | 1.2 |
Cytoplasm | 0.4 | -3.5 | -4.8 | -7.8 | -8.2 | -10.4 | – | -11.6 | -12.4 | -0.1 | 1.7 | 2.2 | -3.8 | -2.7 | -3.5 |
Endoplasmic Reticulum | -0.3 | 0.3 | -0.2 | 0.4 | 0.3 | 0.2 | – | 1.5 | 1.1 | -0.6 | -1.7 | -2.3 | 1.0 | 0.7 | 0.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.7 | 5.7 | 9.1 | 11.0 | 11.0 | 14.2 | – | 15.9 | 16.5 | 0 | -0.8 | 3.8 | -0.6 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0.6 | 0 | 0 | 0.8 | 1.5 | – | 0.4 | 1.2 | 0 | 0 | 0 | 4.6 | 5.4 | 6.0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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