Standard name
Description Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 – 0 0.05 0 0.05 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.91 0.96 0.93 0.94 0.85 0.82 0.72 0.7 0.61 – 0.57 0.54 0.93 0.96 0.97 0.84 0.86 0.84 0.91 0.93 0.87 0.87 0.87 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0.06 0.05 0 0 0 0.05 0.05 0 0 0 0 0.07 0.06 0.14
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.15 0.26 0.33 0.33 0.48 – 0.56 0.56 0 0 0.1 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.13 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 2 0 3 7 3 4 2 – 4 5 3 2 1 3 1 3 0 0 2 0 0 1
Bud 0 2 6 1 2 5 5 5 6 – 7 8 1 0 3 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 26 6 10 3 14 8 16 6 14 – 16 28 1 12 6 5 7 11 0 0 0 0 0 0
Cytoplasm 458 370 331 279 286 369 346 375 251 – 243 296 188 245 273 274 168 200 519 369 331 151 180 172
Endoplasmic Reticulum 18 14 12 9 13 14 19 20 15 – 24 27 5 3 2 16 9 9 12 7 15 12 12 31
Endosome 1 0 0 0 0 0 2 1 0 – 0 0 3 1 0 5 1 1 2 0 1 0 1 2
Golgi 3 1 1 0 0 0 0 0 0 – 0 0 0 0 1 3 3 2 0 0 2 0 1 1
Mitochondria 5 0 9 35 49 117 161 176 199 – 239 307 3 4 27 6 1 3 8 1 9 2 1 1
Nucleus 2 1 1 0 0 0 2 2 1 – 0 3 0 2 0 5 3 3 2 4 1 0 0 0
Nuclear Periphery 0 2 0 0 0 0 1 0 0 – 0 1 0 0 1 1 0 0 3 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Vac/Vac Membrane 17 4 6 0 8 5 8 13 14 – 9 16 6 2 0 32 25 34 17 10 10 3 5 5
Unique Cell Count 504 385 355 297 336 451 481 532 412 430 546 202 254 281 326 195 237 569 399 382 174 208 224
Labelled Cell Count 532 402 378 327 375 525 563 602 502 542 691 210 271 315 350 220 267 569 399 382 174 208 224


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 11.6 8.4 7.7 8.0 7.2 6.9 6.4 6.1 – 5.8 5.6 9.9 9.7 8.9 11.5 12.9 12.1 10.9 11.9 12.3
Std Deviation (1e-4) 4.5 1.9 1.3 1.1 1.4 1.1 1.3 1.1 1.3 – 1.2 1.1 1.5 1.5 1.4 2.8 3.0 3.2 1.8 2.1 2.2
Intensity Change (Log2) – – – -0.13 -0.07 -0.21 -0.28 -0.39 -0.46 – -0.52 -0.59 0.24 0.2 0.08 0.46 0.63 0.53 0.38 0.51 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 -0.3 – -0.1 -0.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -1.6 1.0 -1.0 0.4 -1.9 0.5 – 0.7 1.7 0 1.2 -0.5 -1.1 0.5 1.2
Cytoplasm 0.4 -3.5 -4.8 -7.8 -8.2 -10.4 – -11.6 -12.4 -0.1 1.7 2.2 -3.8 -2.7 -3.5
Endoplasmic Reticulum -0.3 0.3 -0.2 0.4 0.3 0.2 – 1.5 1.1 -0.6 -1.7 -2.3 1.0 0.7 0.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 4.7 5.7 9.1 11.0 11.0 14.2 – 15.9 16.5 0 -0.8 3.8 -0.6 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0.6 0 0 0.8 1.5 – 0.4 1.2 0 0 0 4.6 5.4 6.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ski2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available