Standard name
Human Ortholog
Description Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.06 0.13 0.8 0.73 0.65 0.66 0.66 0.55 0.6 0.5 0.6 0.64 0.69 0.82 0.67 0.73 0 0 0.08 0.18 0.26 0.26
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.06 0 0.16 0.1 0.27 0.17 0 0 0 0 0.06 0 0 0 0 0 0
Nucleus 0.76 0.59 0.61 0 0 0 0.1 0.06 0.12 0.09 0.08 0.07 0.18 0.06 0 0.1 0.19 0.65 0.63 0.58 0.48 0.37 0.34
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.14 0.07 0.06 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.08
Vac/Vac Membrane 0.11 0.32 0.25 0.29 0.33 0.26 0.25 0.29 0.28 0.32 0.21 0.2 0.35 0.39 0.33 0.38 0.23 0 0.07 0.12 0.06 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 4 0
Bud 0 0 0 0 0 0 0 0 1 2 4 1 0 0 0 0 0 1 13 7 11 12 28
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 6
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1 1 1 0 1 1 3 0 3 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 14 16 63 120 131 152 209 111 148 126 156 136 253 322 46 38 1 8 14 40 89 105
Endoplasmic Reticulum 0 0 1 0 0 4 1 1 3 1 4 0 0 2 0 2 3 0 0 1 1 3 1
Endosome 0 11 4 2 3 7 9 10 4 6 2 8 9 19 13 3 0 4 11 3 7 20 18
Golgi 1 0 0 0 2 0 1 0 0 0 0 0 0 1 0 0 1 1 0 0 0 1 1
Mitochondria 5 3 0 2 1 18 13 14 33 24 67 44 0 1 1 2 3 2 1 2 1 5 6
Nucleus 86 139 75 1 3 9 23 19 25 21 20 19 39 22 6 7 10 85 209 99 106 126 140
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 2 1
Nucleolus 13 11 2 1 1 0 0 0 0 1 2 1 0 0 1 0 0 21 47 11 12 17 22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 1 0 4 10 6 16 25 31
Vac/Vac Membrane 12 76 30 23 54 53 58 91 56 79 52 52 74 143 129 26 12 3 23 21 13 25 30
Unique Cell Count 113 235 122 79 165 203 232 316 202 247 252 258 213 369 395 69 52 132 331 173 221 346 410
Labelled Cell Count 124 254 130 93 185 223 257 346 234 286 277 285 258 441 472 87 67 132 331 173 221 346 410


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 10.4 9.4 9.0 10.0 8.3 7.8 8.1 7.0 7.4 6.7 7.0 9.5 9.6 9.3 9.6 9.8 8.4 8.9 8.8
Std Deviation (1e-4) 1.2 2.2 1.5 2.8 3.8 3.3 2.3 1.8 1.9 1.6 1.8 1.8 1.4 1.6 1.1 1.9 1.8 1.2 1.4 1.6
Intensity Change (Log2) -0.07 0.1 -0.19 -0.27 -0.21 -0.42 -0.34 -0.5 -0.42 0.02 0.03 -0.02 0.03 0.07 -0.15 -0.08 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9.4 10.0 9.0 9.4 10.0 7.5 8.5 6.9 8.7 9.0 10.7 13.9 7.6 7.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 0 0 4.7 3.6 6.3 4.9 0 0 0 0 0
Nucleus -8.6 -11.2 -11.4 -10.3 -12.7 -9.3 -10.9 -11.2 -11.4 -8.0 -13.4 -15.9 -6.9 -5.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 1.5 0.3 0.1 0.9 0.6 1.5 -0.9 -1.0 1.9 2.8 1.7 1.9 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0712 5.2395 4.3135 4.0603 3.8928 4.2968 6.9726 6.549 7.1212 6.9693 6.3461 6.5196 7.7955 7.6208 7.3661 6.8206 6.7233 6.9966
Actin 0.0071 0.0003 0.0032 0.0094 0.0033 0.0022 0 0.0001 0 0 0.0002 0.0001 0 0.0001 0 0 0 0
Bud 0.0005 0.0006 0.0011 0.0001 0.0015 0.0005 0 0.0002 0.0002 0 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0001
Bud Neck 0.0021 0.0007 0.0023 0.0008 0.0013 0.0039 0.0002 0.0009 0.0003 0.0003 0.0007 0.002 0.0002 0.0006 0.0003 0.0005 0.0006 0.0012
Bud Periphery 0.0009 0.0004 0.0009 0.0001 0.0061 0.0003 0 0.0001 0 0 0.0001 0 0 0.0001 0 0 0 0
Bud Site 0.0034 0.0017 0.0057 0.0004 0.0009 0.0006 0.0001 0.0005 0.0004 0.0001 0.0002 0.0002 0.0002 0.0008 0.0003 0.0001 0 0.0001
Cell Periphery 0.0003 0.0001 0.0003 0 0.0006 0.0001 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0422 0.0824 0.096 0.1622 0.0211 0.0696 0.0378 0.0652 0.0366 0.0333 0.0703 0.0361 0.0317 0.0664 0.0522 0.0574 0.0734 0.0598
Cytoplasmic Foci 0.0249 0.0042 0.011 0.0304 0.0183 0.0062 0.0003 0.0009 0.0003 0 0.0022 0.0008 0.0002 0.0009 0.0004 0.0007 0.0003 0.0007
Eisosomes 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0025 0.0009 0.0029 0.0015 0.0059 0.0029 0.0004 0.0011 0.0006 0.0002 0.0011 0.0005 0.0002 0.0006 0.0005 0.0005 0.0005 0.0005
Endosome 0.0224 0.0019 0.0243 0.016 0.0146 0.0147 0.0004 0.0024 0.0007 0.0001 0.0034 0.0011 0.0003 0.0016 0.0014 0.0008 0.0005 0.0009
Golgi 0.0064 0.0001 0.0061 0.0123 0.0058 0.0037 0 0 0 0 0.0003 0 0 0 0.0001 0 0 0
Lipid Particles 0.005 0.0001 0.0038 0.0213 0.0063 0.003 0 0 0 0 0 0 0 0 0.0001 0 0 0
Mitochondria 0.0084 0.0005 0.0049 0.0019 0.0437 0.0037 0.0001 0.0003 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0006 0.0001 0.0001 0.0001
None 0.0017 0.0006 0.0122 0.0024 0.001 0.001 0.0009 0.0011 0.0006 0.0001 0.0049 0.0006 0.0004 0.0005 0.0004 0.0008 0.0005 0.0005
Nuclear Periphery 0.0183 0.0127 0.0151 0.013 0.0158 0.0176 0.0049 0.0109 0.0046 0.0037 0.0067 0.0052 0.0041 0.0067 0.0046 0.0041 0.0091 0.0046
Nucleolus 0.0052 0.0022 0.0041 0.0007 0.0044 0.0042 0.0012 0.0029 0.0014 0.0013 0.0025 0.003 0.0011 0.0019 0.001 0.0012 0.0016 0.0018
Nucleus 0.8261 0.8674 0.7847 0.7163 0.8056 0.8301 0.9481 0.8997 0.9462 0.9585 0.8992 0.9388 0.9587 0.9101 0.9353 0.923 0.9047 0.9244
Peroxisomes 0.0042 0.0001 0.0023 0.0045 0.0233 0.003 0 0.0001 0 0 0.0002 0 0 0 0 0 0 0
Punctate Nuclear 0.01 0.0207 0.0122 0.0051 0.0071 0.0278 0.0053 0.0116 0.0072 0.002 0.0061 0.0106 0.0023 0.0077 0.0023 0.0104 0.0082 0.0046
Vacuole 0.0062 0.0022 0.0055 0.0013 0.0083 0.0038 0.0002 0.0016 0.0005 0.0002 0.0011 0.0005 0.0003 0.0014 0.0005 0.0004 0.0004 0.0005
Vacuole Periphery 0.0019 0.0003 0.0013 0.0004 0.0051 0.0011 0 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.9711 30.2835 21.7583 31.4495 25.7956 19.8847 32.0777 30.5072 27.3114 35.6492
Translational Efficiency 1.224 0.8356 1.1838 0.5781 0.7877 1.4831 0.9592 1.0149 1.0828 0.9513

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency