Standard name
Human Ortholog
Description Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1; upstream component for regulation through the small GTPase Rho5p; may form a complex with Lmo1p that acts as a GEF for Rho5p; interacts with Ino4p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein; DOCK proteins act as guanine nucleotide exchange factors
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.09 0.08 0.11 0.09 0.12 0.15
Bud 0 0 0 0.05 0 0.07 0.06 0.06 0.05 0.08 0.07 0.09 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.07 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.1 0.06 0 0.06 0 0 0 0 0.07 0 0.07 0.05 0.16 0.2 0.2 – – – 0.05 0.06 0.08 0.05 0.1 0.17
Cytoplasm 0.9 0.94 0.96 0.95 0.91 0.85 0.8 0.8 0.79 0.76 0.74 0.72 0.97 0.97 0.96 – – – 0.56 0.62 0.52 0.69 0.57 0.46
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.11 0 0 0 0.06 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.06 0.15 0.21 0.18 0.26 0.29 0.28 0.31 0 0 0 – – – 0.05 0.05 0.14 0.06 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 4 4 6 4 14 9 4 2 4 2 4 7 6 5 – – – 31 15 56 28 54 85
Bud 2 2 22 20 19 46 49 50 31 56 30 41 7 4 21 – – – 7 1 7 4 11 7
Bud Neck 0 0 11 7 13 18 8 20 9 11 8 9 0 0 1 – – – 12 14 16 10 17 27
Bud Site 0 0 0 1 9 10 10 17 13 11 6 4 0 0 1 – – – – – – – – –
Cell Periphery 31 12 16 22 19 24 17 19 41 26 31 24 63 95 111 – – – 15 11 41 16 45 94
Cytoplasm 274 185 509 364 501 596 626 671 475 538 318 325 386 456 535 – – – 190 127 269 223 262 254
Endoplasmic Reticulum 1 5 2 1 0 2 1 1 1 1 1 2 1 3 4 – – – 38 6 16 5 25 32
Endosome 6 0 0 0 0 0 0 1 0 0 0 0 1 1 4 – – – 7 4 7 2 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 4 0 2 1 0 1
Mitochondria 11 4 5 25 34 108 162 153 159 202 119 141 0 1 4 – – – 17 9 72 17 24 29
Nucleus 0 1 0 0 0 2 1 4 2 5 1 4 0 1 1 – – – 0 1 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 4 0 0 0 0 0 0 0 0 0 0 0 0 2 2 – – – 1 0 1 0 0 1
SpindlePole 5 0 6 1 14 11 19 26 19 15 5 4 2 0 2 – – – 1 0 3 0 0 2
Vac/Vac Membrane 3 0 0 0 0 0 0 4 1 1 0 3 0 1 2 – – – 3 8 6 6 7 6
Unique Cell Count 305 197 532 385 548 704 784 836 601 705 428 450 397 471 557 338 206 521 323 462 557
Labelled Cell Count 342 214 575 447 613 831 903 971 753 871 521 562 467 570 693 338 206 521 323 462 557


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.4 3.8 3.6 3.8 3.5 3.5 3.6 3.2 3.1 3.2 3.2 5.6 5.7 5.1 – – – 5.7 5.9 6.2
Std Deviation (1e-4) 1.0 0.7 0.8 0.9 1.1 0.8 0.9 1.3 1.1 0.9 0.9 1.1 0.9 1.1 1.0 – – – 1.7 1.5 1.4
Intensity Change (Log2) – – – -0.09 0.01 -0.11 -0.13 -0.06 -0.25 -0.28 -0.26 -0.26 0.57 0.59 0.42 – – – 0.6 0.63 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 -2.1 -3.3 -0.3 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 -3.1 -3.0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 7.0 8.6 8.7 – – –
Cytoplasm -0.8 -2.8 -6.2 -8.2 -8.1 -8.3 -9.4 -9.5 -10.2 1.2 0.9 0.3 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6452 3.0299 2.6697 2.5678 2.7927 2.727 0.4399 0.9277 0.5499 0.2875 0.8252 0.5987 0.2613 1.3192 0.9019 0.9028 1.2807 0.866
Actin 0.0468 0.028 0.0185 0.0873 0.0306 0.0128 0.0337 0.0057 0.0328 0.0336 0.0366 0.0299 0.0212 0.0035 0.0526 0.0296 0.0144 0.0238
Bud 0.0017 0.0011 0.0047 0.0016 0.0017 0.0021 0.0044 0.0034 0.0045 0.016 0.0076 0.0015 0.0014 0.0024 0.0011 0.0012 0.0006 0.0013
Bud Neck 0.0137 0.0029 0.0055 0.0031 0.0221 0.0246 0.012 0.0032 0.0137 0.0077 0.0086 0.0281 0.0017 0.0012 0.0139 0.0096 0.0018 0.0245
Bud Periphery 0.0054 0.0023 0.012 0.0059 0.0068 0.0049 0.0102 0.0085 0.0112 0.0674 0.022 0.0041 0.0033 0.0049 0.0019 0.003 0.0009 0.0071
Bud Site 0.0089 0.0714 0.0335 0.0103 0.0427 0.0017 0.0364 0.1604 0.047 0.0116 0.0214 0.0028 0.0194 0.0523 0.0198 0.0034 0.002 0.0009
Cell Periphery 0.0259 0.0162 0.0147 0.0057 0.0186 0.0089 0.0107 0.0148 0.0075 0.0044 0.0031 0.0053 0.0051 0.0054 0.0036 0.0014 0.0006 0.003
Cytoplasm 0.4552 0.5924 0.5785 0.4055 0.4185 0.614 0.6292 0.6628 0.7009 0.6831 0.6 0.7659 0.6732 0.7463 0.7451 0.7673 0.806 0.792
Cytoplasmic Foci 0.0202 0.0077 0.0116 0.0163 0.0289 0.0073 0.0202 0.0053 0.0177 0.0109 0.0363 0.0172 0.0246 0.0064 0.0092 0.0074 0.0243 0.0138
Eisosomes 0.0016 0.0008 0.001 0.0006 0.0019 0.0004 0.0006 0.0004 0.0004 0.0002 0.0003 0.0003 0.0003 0.0001 0.0003 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.0096 0.0024 0.0052 0.0051 0.0065 0.0026 0.0073 0.0036 0.0045 0.0062 0.0095 0.0023 0.0045 0.0042 0.0062 0.0051 0.004 0.0075
Endosome 0.0261 0.001 0.0021 0.0015 0.0266 0.0021 0.036 0.0008 0.0085 0.0045 0.0412 0.0068 0.0085 0.0025 0.0083 0.0065 0.046 0.0035
Golgi 0.007 0.0033 0.0033 0.0022 0.0104 0.0013 0.011 0.0007 0.0055 0.0022 0.0374 0.0049 0.0025 0.001 0.0052 0.0023 0.008 0.0014
Lipid Particles 0.0081 0.0006 0.0033 0.0024 0.012 0.0023 0.0099 0.0012 0.0024 0.0004 0.0351 0.0014 0.0056 0.0004 0.0024 0.0019 0.0196 0.0004
Mitochondria 0.0179 0.0023 0.0014 0.0026 0.0048 0.0014 0.0078 0.0002 0.0043 0.0012 0.0135 0.007 0.0014 0.0002 0.0019 0.0023 0.0048 0.0005
None 0.3047 0.2602 0.2948 0.4193 0.3346 0.3091 0.1377 0.1254 0.1285 0.141 0.0668 0.1185 0.1945 0.1657 0.1206 0.1447 0.0129 0.1175
Nuclear Periphery 0.0085 0.0002 0.0005 0.0005 0.0068 0.0003 0.006 0.0003 0.0008 0.0007 0.0071 0.0003 0.0013 0.0003 0.0017 0.0025 0.0146 0.0003
Nucleolus 0.0005 0.0002 0.0004 0.0003 0.0019 0.0002 0.001 0.0002 0.0006 0.0003 0.0145 0.0001 0.0018 0.0001 0.0001 0.0009 0.0014 0
Nucleus 0.0082 0.0004 0.0008 0.0007 0.0024 0.0007 0.0034 0.001 0.0014 0.0019 0.0065 0.0005 0.0055 0.0006 0.0007 0.0022 0.0033 0.0003
Peroxisomes 0.0223 0.0053 0.0054 0.026 0.007 0.0021 0.008 0.0001 0.0037 0.0008 0.0173 0.0013 0.0071 0.0001 0.0028 0.0016 0.0029 0.0011
Punctate Nuclear 0.0023 0.0002 0.0012 0.001 0.0075 0.0004 0.0022 0.0005 0.0009 0.0007 0.0063 0.0006 0.0096 0.0002 0.0005 0.0055 0.0259 0.0003
Vacuole 0.0045 0.0012 0.0014 0.0022 0.0066 0.0009 0.0102 0.0015 0.0029 0.0046 0.0073 0.0011 0.0071 0.002 0.0017 0.0011 0.0031 0.0005
Vacuole Periphery 0.0011 0 0.0001 0.0001 0.001 0.0001 0.0019 0.0001 0.0003 0.0005 0.0014 0.0002 0.0004 0.0001 0.0004 0.0003 0.0027 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.888 10.5458 14.2625 19.7442 15.9121 16.3546 17.4562 19.6169 18.2984 20.5759
Translational Efficiency 0.25 0.2973 0.2836 0.2309 0.1901 0.2203 0.2238 0.1891 0.2012 0.1783

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1; upstream component for regulation through the small GTPase Rho5p; may form a complex with Lmo1p that acts as a GEF for Rho5p; interacts with Ino4p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein; DOCK proteins act as guanine nucleotide exchange factors
Localization
Cell Percentages cytoplasm (35%), ER (3%), mixed (43%)
Cell Cycle Regulation Yes
ER - S/G2 (0.00093)
Subcompartmental Group N/A

Dck1

Dck1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dck1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available