Standard name
Human Ortholog
Description Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.08 0.07 0 0.09 0.08 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.96 0.97 0.85 0.79 0.66 0.59 0.56 0.51 0.49 0.38 0.41 0.96 0.97 0.93 0.92 0.95 0.93 0.77 0.87 0.67 0.88 0.77 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.07 0.08 0.45 0.44 0.41 0.56 0.56 0.74 0.65 0 0 0 0 0 0 0.1 0 0.17 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.08 0.09 0.06 0.1 0.14 0.12 0.12 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 2 0 0 0 0 0 1 1 1 0 0 0 4 2 5 0 2 2
Bud 1 2 2 4 16 22 16 26 24 22 21 14 1 0 0 3 0 1 1 1 1 2 7 8
Bud Neck 1 1 0 0 4 4 5 2 3 6 1 1 7 10 10 1 1 2 2 0 1 1 2 1
Bud Site 0 0 0 0 0 0 2 2 0 4 6 3 0 0 0 0 0 0
Cell Periphery 1 0 2 0 1 1 0 1 1 0 1 3 1 1 4 13 10 6 2 0 1 1 0 2
Cytoplasm 336 231 266 107 154 203 215 161 154 132 105 130 392 518 458 349 249 305 279 209 186 273 210 299
Endoplasmic Reticulum 1 3 1 0 0 0 0 0 0 1 0 1 3 2 5 16 18 28 2 1 3 0 1 2
Endosome 0 0 2 4 10 4 7 9 8 4 3 7 3 1 5 13 2 6 9 4 14 6 10 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 3 0 0 2 0 0 0
Mitochondria 41 8 2 9 16 139 161 118 168 150 204 206 0 0 2 3 6 9 36 9 47 11 6 19
Nucleus 0 0 0 3 1 0 2 1 1 0 1 1 0 2 0 2 0 0 0 0 0 1 4 0
Nuclear Periphery 1 0 0 2 0 0 0 0 0 3 0 2 0 0 0 0 0 1 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 1 3 6 3 3 7 3 7 6 12 0 0 1 1 0 1 0 0 0 0 0 0
SpindlePole 4 1 4 10 17 19 37 39 36 33 21 28 7 12 20 3 2 6 8 2 7 4 6 13
Vac/Vac Membrane 0 0 1 0 1 2 3 0 2 1 6 4 1 2 2 8 6 7 5 2 1 3 8 4
Unique Cell Count 364 240 275 126 194 308 366 287 302 270 276 316 407 535 495 378 262 329 363 242 280 312 274 370
Labelled Cell Count 386 246 281 142 227 397 453 367 400 363 375 414 416 549 508 414 296 375 363 242 280 312 274 370


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.8 3.9 3.7 3.8 3.0 3.1 3.3 3.0 3.0 2.8 2.7 5.3 5.4 5.5 6.2 5.6 5.5 5.5 6.1 6.1
Std Deviation (1e-4) 0.4 0.6 1.4 1.7 1.8 1.1 1.4 1.8 1.0 1.2 0.9 0.7 1.0 1.2 1.4 1.8 1.6 1.8 1.0 1.8 1.7
Intensity Change (Log2) -0.07 -0.06 -0.37 -0.32 -0.26 -0.38 -0.4 -0.51 -0.52 0.44 0.46 0.48 0.65 0.51 0.49 0.5 0.63 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6084 0.1232 -0.1818 -0.431 -0.3608 -0.0126 0.8304 0.2855 0.1235 0.254 0.2508 0.0362 -0.8129 -0.722 -0.6844 -0.5577 -1.2324 -0.7332
Actin 0.0006 0.0006 0.0002 0.0022 0.0004 0.0006 0.0433 0.0028 0.0041 0.0596 0.0038 0.0018 0.024 0.0016 0.0012 0.0003 0.0014 0.0001
Bud 0.0003 0.0007 0.0002 0.0001 0.0015 0.0002 0.0022 0.0018 0.001 0.0007 0.0004 0.0016 0.0006 0.0066 0.0002 0.0094 0.0077 0
Bud Neck 0.0002 0.0003 0 0.0001 0.0004 0.0003 0.0043 0.0002 0.0012 0.0155 0.0015 0.0011 0.0022 0.0001 0.0004 0.0002 0.0004 0.0001
Bud Periphery 0.0004 0.0007 0.0004 0.0002 0.0025 0.0003 0.0049 0.0023 0.0051 0.0014 0.0003 0.0044 0.0006 0.0047 0.0001 0.0025 0.0064 0
Bud Site 0.0014 0.01 0.0007 0 0.0007 0.0001 0.0114 0.0039 0.0006 0.0018 0.0047 0.0007 0.0032 0.0027 0.0022 0.0016 0.0014 0
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0009 0.0002 0.0001 0.0002 0.0001 0.0001 0.0005 0.0002 0.0002 0.0003 0.0002 0
Cytoplasm 0.022 0.0238 0.0407 0.0404 0.0364 0.0395 0.0395 0.0676 0.0852 0.0518 0.0585 0.0833 0.0268 0.0674 0.0419 0.0423 0.036 0.0811
Cytoplasmic Foci 0.0168 0.015 0.0187 0.0443 0.0303 0.0172 0.0609 0.0051 0.012 0.033 0.0454 0.0167 0.0095 0.0072 0.0045 0.0521 0.0137 0.0193
Eisosomes 0.0003 0.0004 0.0002 0.0004 0.0001 0.0001 0.0005 0.0001 0.0001 0.0003 0.0001 0.0001 0.0005 0.0001 0.0003 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0004 0.0004 0.0005 0.0002 0.0002 0.0036 0.0003 0.0029 0.0021 0.0013 0.0006 0.002 0.0004 0.0016 0.0007 0.0012 0.0005
Endosome 0.0044 0.0017 0.0053 0.0042 0.0067 0.0029 0.0544 0.0007 0.02 0.0378 0.0598 0.0131 0.0107 0.0031 0.001 0.0065 0.0003 0.0085
Golgi 0.0004 0.0003 0.0002 0.0003 0.0013 0.0001 0.0195 0.0006 0.0126 0.0115 0.0169 0.0039 0.0034 0.0001 0.0001 0.0006 0.0002 0.0003
Lipid Particles 0.0016 0.0006 0.0005 0.0027 0.0004 0.0003 0.0387 0.002 0.0041 0.0284 0.0228 0.0026 0.0045 0.0003 0.0007 0.0035 0.0008 0.0004
Mitochondria 0.0003 0.0003 0.0003 0.0006 0.0197 0.0003 0.0127 0.0007 0.0056 0.0352 0.0026 0.0042 0.0016 0.0006 0.0004 0.001 0.0015 0.0002
None 0.9413 0.9406 0.9294 0.8972 0.8911 0.9355 0.6214 0.9032 0.8347 0.693 0.7625 0.8478 0.8788 0.9026 0.9328 0.8551 0.9057 0.8867
Nuclear Periphery 0.0044 0.0002 0.0003 0.0008 0.0001 0.0001 0.0063 0.0003 0.0024 0.0054 0.0014 0.0005 0.0114 0.0002 0.004 0.0009 0.0017 0.0002
Nucleolus 0.0002 0.0006 0.0001 0.0002 0.0003 0.0001 0.0018 0.0004 0.0003 0.0003 0.0004 0.0002 0.0011 0.0003 0.0004 0.0015 0.0013 0.0001
Nucleus 0.0011 0.0008 0.0005 0.0005 0.0003 0.0003 0.0023 0.001 0.0009 0.0005 0.001 0.0008 0.0077 0.0006 0.0056 0.0029 0.003 0.0003
Peroxisomes 0.0012 0.0007 0.0003 0.0022 0.0054 0.0005 0.0538 0.0034 0.0028 0.0151 0.01 0.0065 0.0015 0.0001 0.0001 0.0019 0.0007 0.0003
Punctate Nuclear 0.0019 0.0016 0.0007 0.0019 0.0012 0.0008 0.0102 0.0028 0.0009 0.0041 0.0041 0.0015 0.0075 0.0004 0.0016 0.015 0.0151 0.001
Vacuole 0.0003 0.0005 0.0006 0.0009 0.0006 0.0002 0.0055 0.0004 0.0023 0.0011 0.0019 0.0066 0.0012 0.0008 0.0007 0.0011 0.0009 0.0005
Vacuole Periphery 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0019 0.0001 0.001 0.001 0.0006 0.002 0.0007 0.0001 0.0002 0.0003 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.6397 2.3598 4.6694 3.1226 3.4225 5.2765 4.1754 4.5627 3.0822 5.8008
Translational Efficiency 1.1822 1.5508 0.5308 0.8339 1.3009 1.0048 0.8527 0.6188 1.1843 0.632

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1236 236 2327 1029 1724 1599 229 678 2960 1835 2556 1707

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 618.26 755.28 769.67 783.63 633.94 686.41 869.58 779.44 627.39 695.27 778.62 781.97
Standard Deviation 65.84 95.26 87.63 100.38 72.51 81.82 93.81 112.33 70.23 86.79 92.70 105.31
Intensity Change Log 2 0.288798 0.316026 0.341959 0.114724 0.455972 0.298092 0.203296 0.388571 0.319917

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000148 0.000867 0.000171 0.000915 0.000057 0.000485 0.000265 0.000628 0.000095 0.000534 0.000180 0.000801
Bud Neck 0.017898 0.033066 0.013134 0.007437 0.011931 0.038097 0.002585 0.006883 0.014423 0.037450 0.012189 0.007217
Bud Site 0.003566 0.014711 0.016724 0.031631 0.002836 0.017569 0.002540 0.015318 0.003141 0.017201 0.015453 0.025152
Cell Periphery 0.000147 0.000230 0.000135 0.000220 0.000114 0.000132 0.000224 0.000174 0.000128 0.000144 0.000143 0.000202
Cytoplasm 0.399082* 0.308792* 0.507175* 0.435305* 0.544330* 0.401178* 0.290277* 0.478150* 0.483679* 0.389296* 0.487743* 0.452323*
Cytoplasmic Foci 0.310526* 0.333369* 0.029812 0.043346 0.194349* 0.269368* 0.054848 0.062546 0.242861* 0.277599* 0.032055 0.050972
Eisosomes 0.000281 0.000158 0.000033 0.000080 0.000096 0.000163 0.000134 0.000080 0.000173 0.000162 0.000042 0.000080
Endoplasmic Reticulum 0.001345 0.000754 0.001031 0.003773 0.000773 0.000552 0.003362 0.002220 0.001012 0.000578 0.001240 0.003156
Endosome 0.019299 0.038273 0.006265 0.011190 0.006950 0.028853 0.011087 0.010102 0.012107 0.030064 0.006697 0.010758
Golgi 0.003939 0.005273 0.000155 0.003509 0.001423 0.005412 0.000138 0.001880 0.002474 0.005394 0.000153 0.002862
Lipid Particles 0.019449 0.006809 0.001394 0.003057 0.005888 0.006524 0.005427 0.003112 0.011550 0.006560 0.001755 0.003079
Mitochondria 0.009409 0.013357 0.001501 0.002407 0.002623 0.012468 0.000377 0.002928 0.005456 0.012582 0.001401 0.002614
Mitotic Spindle 0.000374 0.012805 0.016344 0.025828 0.001062 0.009626 0.000480 0.022553 0.000775 0.010035 0.014923 0.024527
None 0.001967 0.001174 0.005241 0.004502 0.004784 0.001597 0.005257 0.003631 0.003608 0.001543 0.005243 0.004156
Nuclear Periphery 0.000651 0.000593 0.002101 0.002005 0.000375 0.000417 0.001885 0.001149 0.000490 0.000439 0.002082 0.001665
Nuclear Periphery Foci 0.000268 0.000737 0.000668 0.002670 0.000276 0.000268 0.003950 0.003444 0.000272 0.000329 0.000962 0.002977
Nucleolus 0.001086 0.001949 0.001581 0.001373 0.001276 0.001380 0.001450 0.001033 0.001197 0.001453 0.001569 0.001238
Nucleus 0.146311* 0.099501 0.340902* 0.225058* 0.190421* 0.110788 0.478564* 0.193235* 0.172002* 0.109336 0.353235* 0.212419*
Peroxisomes 0.012317 0.034702 0.002362 0.006095 0.003502 0.019354 0.003124 0.002025 0.007183 0.021328 0.002430 0.004478
Vacuole 0.050638 0.089858 0.052835 0.185903 0.026300 0.073746 0.133809 0.187045 0.036463 0.075818 0.060090 0.186356
Vacuole Periphery 0.001298 0.003024 0.000434 0.003695 0.000634 0.002024 0.000220 0.001865 0.000912 0.002152 0.000415 0.002968

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.49 -1.16 -4.06 0.61 -3.76 -6.12 -7.40 -3.39 -0.10 -0.76 -6.15 -4.65 -5.46 -0.54 -4.21
Bud Neck -3.02 1.43 6.49 5.39 5.52 -10.06 4.78 2.82 11.50 -1.31 -9.93 0.00 6.36 13.26 5.53
Bud Site -4.65 -6.44 -6.02 -0.89 -1.23 -8.38 -2.98 -5.09 2.47 -2.79 -9.10 -7.04 -7.76 0.95 -0.86
Cell Periphery -0.86 2.07 -1.04 0.66 -2.42 -1.85 -4.69 -1.25 0.10 3.55 -1.05 0.16 -2.10 -0.47 -1.60
Cytoplasm 5.13 -5.28 5.10 -1.87 10.31 15.26 19.85 14.13 2.55 -7.53 11.57 7.03 15.87 3.92 8.74
Cytoplasmic Foci -1.09 40.49 39.80 19.13 -0.22 -9.79 15.43 23.68 31.35 0.66 -4.86 46.63 44.75 40.35 -2.03
Eisosomes 4.15 10.07 7.97 3.99 -13.11 -7.13 -4.45 2.15 7.95 5.16 0.95 11.76 8.13 9.02 -10.88
Endoplasmic Reticulum 1.53 -0.85 -8.46 -14.38 -12.02 1.44 -9.36 -9.98 -12.72 4.08 2.52 -5.28 -13.41 -19.51 -11.63
Endosome -3.95 7.28 6.34 6.52 -0.99 -12.43 -3.45 -1.48 9.69 2.33 -10.41 4.29 3.50 12.59 -0.67
Golgi -0.65 8.29 3.13 2.13 -1.55 -7.54 7.50 1.16 6.42 -1.21 -5.41 10.87 2.65 6.25 -1.96
Lipid Particles 7.39 11.82 10.73 4.36 -8.40 -1.00 0.44 5.49 6.62 3.72 6.60 13.86 11.97 8.41 -6.27
Mitochondria -0.77 5.84 5.97 2.90 0.82 -6.71 6.28 1.73 7.11 -1.49 -4.76 6.59 6.25 8.22 0.23
Mitotic Spindle -2.30 -8.49 -6.67 -1.58 -1.30 -4.73 -0.64 -4.46 -1.90 -4.35 -5.45 -8.19 -7.92 -3.32 -1.58
None 1.88 -5.01 -2.44 -3.82 1.78 6.64 1.19 2.99 -4.72 1.18 6.24 -2.16 0.48 -4.81 2.42
Nuclear Periphery 0.21 -10.37 -14.30 -12.79 -0.78 -1.04 -12.23 -12.35 -11.94 3.61 0.56 -12.56 -19.04 -19.81 1.44
Nuclear Periphery Foci -3.22 -5.22 -7.71 -6.15 -5.33 0.07 -4.09 -9.38 -9.39 2.01 -1.86 -6.48 -11.46 -11.22 -5.36
Nucleolus -2.54 -4.91 -0.55 1.71 3.91 -0.49 -1.37 0.46 0.92 1.66 -1.45 -4.70 -0.05 1.10 4.39
Nucleus 4.37 -29.12 -10.96 -11.62 14.24 13.00 -11.60 -0.54 -9.25 10.77 12.34 -30.37 -7.90 -17.00 19.50
Peroxisomes -3.54 7.68 6.08 5.13 -0.99 -10.71 -0.91 2.69 11.83 2.30 -8.97 6.79 5.71 11.98 -0.31
Vacuole -5.39 -6.76 -28.41 -16.41 -25.43 -16.17 -12.54 -25.32 -20.56 -4.74 -14.18 -16.31 -38.98 -31.17 -29.47
Vacuole Periphery -2.44 7.59 -0.09 1.68 -1.53 -6.42 8.42 -0.38 2.55 -1.38 -5.86 7.12 -0.52 2.11 -1.83