Standard name
Description Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.98 0.98 0.97 0.94 0.89 – 0.8 0.79 0.67 0.71 0.99 0.99 0.99 – 0.97 0.94 0.98 0.93 0.87 0.92 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.1 0.14 – 0.22 0.25 0.45 0.3 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 4 0 0 1 – 0 0 0 0 3 0 0 0
Bud 0 2 1 0 2 3 2 – 1 4 8 2 1 0 1 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 – 0 0 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 5 4 1 9 6 11 – 6 15 8 14 0 1 6 – 32 32 0 0 1 0 0 0
Cytoplasm 90 150 196 131 284 302 447 – 243 286 218 247 170 212 270 – 347 331 90 144 187 74 176 177
Endoplasmic Reticulum 1 2 0 0 1 0 3 – 0 0 2 20 0 0 2 – 13 14 0 0 3 0 0 0
Endosome 0 0 1 0 0 1 1 – 3 4 5 2 0 1 0 – 0 4 0 0 2 1 0 0
Golgi 0 2 0 0 1 0 1 – 2 0 0 0 0 0 0 – 3 2 0 1 0 0 0 1
Mitochondria 3 1 0 8 8 32 71 – 67 90 147 103 0 0 0 – 3 6 0 1 6 0 3 2
Nucleus 0 0 1 4 1 0 1 – 4 1 1 5 1 1 1 – 1 4 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 1 – 0 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 1 – 0 1 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 0 5 3 7 – 3 21 5 7 0 2 1 – 7 10 0 2 3 2 0 2
Unique Cell Count 91 154 201 133 294 322 501 302 362 326 346 171 215 273 358 351 92 155 215 81 185 190
Labelled Cell Count 95 162 207 144 312 348 546 330 422 398 405 172 217 282 406 404 92 155 215 81 185 190


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.5 7.0 6.4 7.2 5.9 6.2 – 5.5 5.7 5.1 5.4 7.0 7.2 6.8 – 8.6 8.1 5.5 6.5 7.0
Std Deviation (1e-4) 0.6 1.1 1.4 1.1 1.4 1.3 1.1 – 1.4 1.3 1.3 1.0 0.7 1.2 0.9 – 1.5 1.4 0.5 0.9 1.1
Intensity Change (Log2) – – – -0.12 0.03 -0.24 -0.18 – -0.35 -0.29 -0.46 -0.38 -0.0 0.04 -0.04 – 0.29 0.21 -0.35 -0.11 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0.7 0 0 – 0 1.4 0 1.3 0 0 0 – 3.2 3.3
Cytoplasm 0.6 -0.6 -1.9 -3.6 – -5.6 -6.0 -8.3 -7.5 1.5 0.8 1.2 – -0.4 -1.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 3.5 0 0 0 – 0 2.9
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 4.6 5.6 – 7.2 7.7 11.2 8.6 0 0 0 – 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0 0 0 – 0 2.4 0 0 0 0 0 – 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mag2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available