Standard name
Human Ortholog
Description ATP-dependent 5' to 3' RNA/DNA and DNA helicase; subunit of the exosome-associated Nrd1p complex that mediates 3' end formation of snRNAs, snoRNAs, CUTs and some mRNAs; helicase-independent role in transcription-coupled repair; coordinates replication with transcription, associating with moving forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of juvenile ALS

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0.07 0.06 0.07 0.07 0.06 0.07 0.1 0.17 0.19 0.08 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.16 0.26 0.39 0.19 0.41 0.51 0.62 0.77 0.74 0.84 0 0 0.05 0.05 0 0.09 0 0 0 0 0 0
Nucleus 0.94 0.9 0.88 0.84 0.86 0.77 0.72 0.64 0.6 0.6 0.54 0.87 0.8 0.77 0.85 0.87 0.89 0.9 0.9 0.83 0.77 0.63 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.13 0.22 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0
Bud 0 0 0 0 4 1 7 8 13 13 7 0 0 0 0 1 2 2 3 3 2 1 2
Bud Neck 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 1 3 5 13 17 26 29 21 19 0 0 1 1 0 0
Cell Periphery 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 12 11 18 23 20 43 42 58 35 20 30 29 38 44 58 48 51 1 1 3 7 0 1
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 1 1 0 0 12 16 17 1 0 1 0 2 0
Endosome 1 0 0 0 3 0 1 0 0 0 0 1 1 0 7 7 3 5 3 2 2 3 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 1 2 0 2 0 0 2
Mitochondria 2 47 110 114 93 240 329 498 406 269 351 6 4 11 35 10 70 2 3 3 1 3 6
Nucleus 275 272 378 250 413 451 466 514 319 217 226 245 176 180 626 582 659 278 277 381 169 162 139
Nuclear Periphery 0 4 1 1 3 4 5 7 2 3 1 2 2 2 27 12 7 1 0 0 0 2 4
Nucleolus 2 2 1 2 7 9 10 10 13 12 3 2 3 8 14 7 8 1 0 3 5 15 14
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 1 1 4 1 10 11 3 3 2 2 1 1 10 4 4 1 1 4 1 4 3
Vac/Vac Membrane 3 7 4 1 12 8 8 14 13 8 14 3 6 6 33 29 22 5 16 45 28 57 56
Unique Cell Count 294 302 429 296 479 589 650 801 530 362 418 281 221 234 737 671 742 309 309 458 220 258 241
Labelled Cell Count 300 343 514 395 564 770 896 1148 834 568 655 292 231 253 825 717 844 309 309 458 220 258 241


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 5.0 4.3 4.2 4.4 4.0 3.9 3.6 3.3 3.2 2.9 5.8 5.6 5.1 7.8 8.3 7.5 6.4 6.1 6.9
Std Deviation (1e-4) 1.1 0.9 0.8 1.1 1.0 1.2 1.9 1.1 1.2 0.9 1.0 1.3 1.4 1.1 2.1 1.8 1.6 2.3 1.3 1.5
Intensity Change (Log2) -0.05 0.02 -0.12 -0.16 -0.25 -0.39 -0.43 -0.59 0.42 0.38 0.25 0.85 0.94 0.8 0.57 0.51 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 0 2.1 1.6 2.1 1.6 0.9 1.9 3.2 5.6 6.2 2.5 2.0 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -8.3 -7.6 -6.7 -10.4 -12.5 -7.4
Nucleus -1.4 -0.8 -4.7 -6.4 -9.0 -9.6 -9.1 -11.0 -0.4 -2.9 -3.8 -1.5 -0.7 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 3.7 2.3 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 0.6 0 1.1 1.8 1.5 2.4 0 0 0 3.3 3.2 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6832 5.1989 4.8328 4.5502 4.2696 4.6689 1.0389 1.0621 0.8838 0.6856 0.5893 0.9669 5.6992 6.1047 5.7857 5.8235 6.0636 5.9695
Actin 0.057 0.0007 0.0008 0 0.0451 0.0009 0.0256 0 0.0099 0 0 0.0026 0.0116 0 0.0026 0.0063 0.0008 0.0003
Bud 0.0007 0 0.0001 0 0.0014 0.0001 0.0004 0 0.0001 0 0 0.0001 0.0003 0 0.0001 0.0001 0.0004 0.0001
Bud Neck 0.0037 0.0003 0.0002 0.0001 0.0024 0.0015 0.0017 0.0002 0.0003 0.0002 0.0002 0.01 0.0007 0.0001 0.0041 0.0001 0.004 0.0011
Bud Periphery 0.001 0.0001 0.0008 0 0.0031 0.0004 0.0008 0 0.0001 0.0001 0 0.0002 0.0007 0 0.0002 0.0001 0.0006 0.0001
Bud Site 0.0092 0.0003 0.0002 0 0.0085 0.0002 0.0008 0.0002 0.0003 0 0 0.0002 0.002 0.0001 0.0006 0.0001 0.0119 0.0001
Cell Periphery 0.0007 0 0.0002 0 0.0012 0.0003 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0.0003 0
Cytoplasm 0.0197 0.0002 0.0001 0 0.0015 0.0001 0.0021 0.0003 0.0001 0 0 0.0002 0.0039 0.0003 0.001 0.0014 0.0009 0.0021
Cytoplasmic Foci 0.014 0.0002 0.0002 0 0.0141 0.0003 0.0075 0.0014 0.001 0 0 0.0007 0.0046 0 0.0004 0.0061 0.0002 0.0002
Eisosomes 0.0007 0 0.0001 0 0.0009 0 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0048 0.0001 0.0008 0 0.0029 0.0002 0.0024 0 0.0002 0 0 0.0004 0.0028 0.0001 0.0006 0.0011 0.001 0.0006
Endosome 0.0125 0.0011 0.0012 0 0.0338 0.0015 0.014 0.0005 0.0011 0 0 0.0042 0.0071 0.0001 0.0014 0.0017 0.0011 0.0024
Golgi 0.0098 0.0011 0.0015 0 0.0393 0.0007 0.0046 0.0001 0.002 0 0 0.0037 0.0026 0 0.0005 0.0035 0.0002 0.0004
Lipid Particles 0.0167 0.0018 0.0023 0 0.041 0.0042 0.0083 0.0006 0.0019 0 0 0.0073 0.0118 0 0.0003 0.0014 0.0001 0.0004
Mitochondria 0.0068 0.0039 0.0079 0.0001 0.0742 0.0115 0.0029 0.0001 0.0008 0.0001 0.0003 0.0062 0.0043 0.0001 0.0013 0.0018 0.0037 0.0003
None 0.0031 0.0001 0.0001 0 0.0013 0.0001 0.0008 0.0001 0 0 0 0.0001 0.0028 0.0001 0.0009 0.006 0.0002 0.0001
Nuclear Periphery 0.0124 0.0031 0.002 0.0004 0.0129 0.0032 0.0238 0.0014 0.0056 0.0003 0.0364 0.0063 0.0206 0.0029 0.0071 0.0344 0.0127 0.0019
Nucleolus 0.0126 0.0103 0.0123 0.0064 0.0133 0.033 0.0184 0.0154 0.0112 0.0116 0.0206 0.0199 0.0093 0.0094 0.0074 0.0025 0.0223 0.0254
Nucleus 0.795 0.9742 0.9667 0.9927 0.6493 0.9364 0.8732 0.9712 0.9539 0.9872 0.9402 0.9349 0.903 0.9849 0.9654 0.9038 0.9327 0.9631
Peroxisomes 0.0095 0.0001 0.0001 0 0.0398 0.0006 0.0016 0.0001 0.0017 0 0 0.0013 0.0035 0 0.0001 0.0274 0 0.0001
Punctate Nuclear 0.0063 0.0016 0.0004 0.0001 0.0042 0.0005 0.0079 0.0081 0.0095 0.0002 0.0017 0.001 0.0056 0.0015 0.0044 0.0011 0.0027 0.0009
Vacuole 0.0025 0.0003 0.0007 0.0001 0.0069 0.0018 0.0017 0.0003 0.0001 0.0001 0 0.0003 0.0014 0.0002 0.0006 0.0005 0.0016 0.0004
Vacuole Periphery 0.0013 0.0004 0.001 0 0.003 0.0022 0.0012 0 0.0001 0.0001 0.0005 0.0005 0.0011 0.0001 0.0009 0.0006 0.0026 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.526 27.8413 30.3879 33.9172 28.7362 30.9625 34.6665 34.0283 31.5369 37.1021
Translational Efficiency 0.4326 0.459 0.4054 0.3927 0.4181 0.499 0.3681 0.3111 0.3636 0.3382

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
662 167 257 1219 106 1618 178 1190 768 1785 435 2409

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.09 875.01 1071.11 1023.88 723.28 806.01 1076.91 1017.54 692.95 812.47 1073.48 1020.75
Standard Deviation 77.00 119.87 136.13 161.55 89.60 108.05 143.19 169.12 79.79 111.04 139.09 165.36
Intensity Change Log 2 0.346702 0.638437 0.573377 0.156243 0.574272 0.492459 0.252241 0.605911 0.532477

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000097 0.000675 0.001031 0.001527 0.000676 0.000208 0.001303 0.000691 0.000177 0.000252 0.001142 0.001114
Bud Neck 0.002408 0.006734 0.011679 0.028083 0.001524 0.007495 0.009628 0.017627 0.002286 0.007424 0.010840 0.022918
Bud Site 0.000527 0.001785 0.001257 0.007324 0.000342 0.001094 0.001485 0.001955 0.000502 0.001159 0.001350 0.004672
Cell Periphery 0.000057 0.000115 0.000045 0.000122 0.000054 0.000063 0.000088 0.000082 0.000056 0.000068 0.000063 0.000103
Cytoplasm 0.013526 0.005069 0.001868 0.007250 0.016061 0.004948 0.001332 0.001780 0.013876 0.004960 0.001649 0.004548
Cytoplasmic Foci 0.001870 0.000685 0.000030 0.004119 0.003717 0.001091 0.000049 0.000435 0.002125 0.001053 0.000038 0.002299
Eisosomes 0.000012 0.000029 0.000038 0.000028 0.000028 0.000014 0.000049 0.000025 0.000014 0.000016 0.000042 0.000026
Endoplasmic Reticulum 0.000807 0.006419 0.006780 0.004841 0.003699 0.004271 0.007973 0.004970 0.001206 0.004472 0.007269 0.004905
Endosome 0.001372 0.000392 0.000180 0.006032 0.000227 0.000738 0.000262 0.000844 0.001214 0.000706 0.000214 0.003469
Golgi 0.000241 0.000157 0.000062 0.011502 0.000078 0.000682 0.000081 0.000404 0.000218 0.000633 0.000070 0.006020
Lipid Particles 0.000309 0.000469 0.000137 0.000321 0.000569 0.000344 0.000232 0.000087 0.000345 0.000355 0.000176 0.000205
Mitochondria 0.001173 0.001546 0.001104 0.007448 0.000479 0.003559 0.001373 0.002090 0.001078 0.003371 0.001214 0.004802
Mitotic Spindle 0.001361 0.002307 0.000091 0.046438 0.000074 0.006986 0.000573 0.007205 0.001183 0.006549 0.000288 0.027058
None 0.007143 0.004927 0.001220 0.004868 0.005151 0.002368 0.002597 0.002433 0.006868 0.002607 0.001784 0.003665
Nuclear Periphery 0.000710 0.000552 0.000359 0.002356 0.000202 0.001808 0.000642 0.000771 0.000640 0.001690 0.000475 0.001573
Nuclear Periphery Foci 0.000258 0.000513 0.000059 0.001619 0.000252 0.000738 0.000129 0.000377 0.000258 0.000717 0.000088 0.001005
Nucleolus 0.005727 0.008474 0.003700 0.006434 0.006998 0.004644 0.004790 0.004319 0.005902 0.005002 0.004146 0.005389
Nucleus 0.961485* 0.956894* 0.967008* 0.846036* 0.957499* 0.956097* 0.963241* 0.950787* 0.960935* 0.956172* 0.965466* 0.897781*
Peroxisomes 0.000314 0.001031 0.000298 0.000322 0.000905 0.000374 0.000296 0.000168 0.000395 0.000436 0.000298 0.000246
Vacuole 0.000431 0.000983 0.002945 0.009593 0.001384 0.001559 0.003759 0.002309 0.000562 0.001505 0.003278 0.005995
Vacuole Periphery 0.000173 0.000242 0.000107 0.003736 0.000081 0.000920 0.000115 0.000641 0.000160 0.000856 0.000110 0.002207

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.33 -10.99 -7.11 -2.53 -1.05 1.05 -2.73 -0.24 -11.87 6.31 -0.94 -10.92 -7.76 -9.00 3.47
Bud Neck -2.82 -8.13 -15.80 -11.14 -8.64 -4.83 -8.28 -12.71 -9.02 -2.13 -4.64 -10.54 -18.51 -14.62 -7.80
Bud Site -1.25 -2.81 -5.46 -2.28 -4.31 -2.72 -6.02 -5.06 -2.87 1.90 -2.29 -5.56 -6.62 -5.05 -2.81
Cell Periphery -1.62 0.66 -3.65 0.55 -6.25 -0.39 -2.76 -1.40 -1.96 2.20 -1.10 -1.90 -3.44 -2.68 -1.68
Cytoplasm 2.86 4.76 3.95 -0.22 -2.05 1.41 1.85 1.84 3.78 -0.10 4.05 5.54 5.07 1.87 -1.76
Cytoplasmic Foci 1.51 2.75 0.42 -1.42 -3.46 0.91 1.26 1.18 1.93 -1.60 1.34 2.95 1.63 0.35 -3.79
Eisosomes -4.59 -8.50 -12.34 -0.54 2.71 1.94 -4.70 0.09 -8.81 7.90 -1.00 -11.88 -9.99 -10.16 7.57
Endoplasmic Reticulum -9.54 -9.16 -15.72 1.35 1.55 -0.25 -4.19 -1.10 -3.07 6.11 -10.82 -13.08 -14.67 -3.37 5.00
Endosome 1.04 1.31 -1.12 -3.60 -4.11 -2.75 -2.94 -1.54 0.25 -0.50 0.61 1.13 -0.50 -2.53 -4.01
Golgi 0.38 0.96 -2.91 -2.97 -3.04 -1.63 -2.37 -1.54 0.96 -0.69 -1.17 0.58 -2.93 -2.18 -3.10
Lipid Particles -0.94 2.71 0.52 1.18 -2.38 0.93 0.71 1.95 3.53 3.23 -0.25 1.38 2.31 2.63 1.15
Mitochondria -0.36 -0.17 -3.17 -2.88 -4.45 -3.71 -6.82 -6.47 1.45 -0.53 -2.23 -0.81 -3.02 -0.25 -4.54
Mitotic Spindle -0.51 1.11 -6.72 -5.96 -7.27 -4.09 -3.28 -4.07 -0.56 -1.63 -3.05 -0.77 -7.25 -4.31 -7.21
None 0.84 3.88 1.81 0.20 -4.96 1.76 1.86 1.62 -0.35 -0.53 2.96 3.88 2.49 -1.24 -4.15
Nuclear Periphery 0.52 0.97 -5.08 -8.02 -8.85 -4.93 -5.97 -9.60 2.12 1.13 -2.80 -0.66 -4.44 -0.42 -6.78
Nuclear Periphery Foci -0.74 0.94 -2.39 -0.51 -4.90 -1.63 -0.13 -0.75 1.16 -0.78 -1.59 0.57 -1.89 0.37 -4.42
Nucleolus -0.98 0.76 -0.44 0.81 -1.22 1.81 -0.49 0.94 -0.93 1.41 0.98 0.06 0.02 -1.20 -0.05
Nucleus 0.60 -0.09 10.06 6.67 11.03 0.08 2.00 1.17 2.65 -1.61 1.01 2.27 8.65 8.59 6.85
Peroxisomes -1.12 -0.13 0.61 1.24 1.21 0.80 0.63 1.09 2.55 4.36 -0.27 -0.00 1.37 2.53 3.69
Vacuole -3.66 -4.39 -7.55 -7.05 -4.53 -0.17 -2.76 -1.86 -2.71 2.44 -3.70 -3.69 -8.62 -6.91 0.82
Vacuole Periphery -0.42 0.48 -2.93 -2.74 -3.12 -1.98 -4.47 -1.82 0.94 -1.31 -1.77 0.11 -3.16 -0.38 -3.41