Standard name | |
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Human Ortholog | |
Description | Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 6 | 1 | 0 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 1 | 2 | 3 | 0 | 0 | 0 | – | 0 | 0 | 5 | 4 | 1 | 3 | 1 | 6 |
Bud Neck | 3 | 3 | 2 | 3 | 0 | 4 | 17 | 15 | 1 | 4 | 4 | 2 | 0 | 2 | 0 | – | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 7 | 5 | 1 | 6 | 1 | 0 | 1 | 0 |
Cytoplasm | 456 | 525 | 140 | 91 | 18 | 191 | 174 | 226 | 130 | 122 | 101 | 119 | 145 | 299 | 263 | – | 302 | 301 | 404 | 439 | 114 | 171 | 134 | 179 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 4 | 1 | – | 20 | 29 | 2 | 1 | 1 | 0 | 0 | 1 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 1 | 7 | 3 | 2 | 1 | 2 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 4 | 1 | 2 | 0 | 1 | 2 | 0 |
Mitochondria | 5 | 11 | 1 | 1 | 3 | 13 | 9 | 13 | 57 | 42 | 50 | 65 | 0 | 0 | 0 | – | 7 | 5 | 27 | 23 | 15 | 2 | 6 | 9 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 1 | 0 | 0 | 2 | 4 | 5 | 3 | 7 | 1 | 4 | 0 | 1 | 0 | – | 2 | 1 | 5 | 1 | 0 | 3 | 2 | 2 |
Vac/Vac Membrane | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 2 | 1 | 9 | 3 | 0 | 2 | 1 | 0 |
Unique Cell Count | 465 | 543 | 145 | 92 | 21 | 200 | 190 | 238 | 164 | 162 | 142 | 168 | 146 | 304 | 265 | 309 | 308 | 480 | 519 | 147 | 196 | 160 | 209 | |
Labelled Cell Count | 472 | 550 | 148 | 96 | 23 | 211 | 208 | 262 | 193 | 178 | 164 | 195 | 147 | 309 | 265 | 346 | 349 | 480 | 519 | 147 | 196 | 160 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.4 | 4.5 | 4.5 | 3.4 | 3.8 | 4.1 | 4.2 | 3.2 | 3.6 | 3.2 | 3.3 | 4.7 | 4.7 | 4.5 | 4.5 | 5.6 | 5.5 | 5.0 | 5.5 | 5.4 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.0 | 1.5 | 0.4 | 0.9 | 1.8 | 1.4 | 1.1 | 1.3 | 0.6 | 0.9 | 1.2 | 1.2 | 1.3 | 1.8 | 2.4 | 1.8 | 1.5 | 2.0 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.39 | -0.24 | -0.15 | -0.1 | -0.51 | -0.32 | -0.51 | -0.46 | 0.07 | 0.07 | -0.02 | -0.01 | 0.3 | 0.3 | 0.16 | 0.29 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9372 | -1.0643 | -1.2502 | -1.2636 | -1.0752 | -1.1221 | 4.4725 | 4.4869 | 4.7621 | 4.1428 | 4.6911 | 5.1434 | 1.8081 | 1.6881 | 1.4952 | 2.2907 | 1.7859 | 1.8901 |
Actin | 0.0466 | 0.0012 | 0.0008 | 0.0001 | 0.0262 | 0.0055 | 0.014 | 0.0001 | 0.0006 | 0 | 0.01 | 0.0038 | 0.0286 | 0.0008 | 0.0049 | 0.0004 | 0.0042 | 0.0059 |
Bud | 0.0027 | 0.0034 | 0.0059 | 0 | 0.0011 | 0.0002 | 0.0053 | 0.0025 | 0.0014 | 0.0002 | 0.0032 | 0.0089 | 0.0003 | 0.0083 | 0.003 | 0.0038 | 0.0004 | 0.0002 |
Bud Neck | 0.0027 | 0.0002 | 0.0001 | 0 | 0.0093 | 0.0003 | 0.0049 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0051 | 0.0005 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0002 |
Bud Periphery | 0.006 | 0.0045 | 0.002 | 0 | 0.003 | 0.0011 | 0.0099 | 0.002 | 0.0029 | 0.0003 | 0.0151 | 0.0125 | 0.0003 | 0.0052 | 0.0008 | 0.0084 | 0.0008 | 0.0004 |
Bud Site | 0.0116 | 0.0027 | 0.0011 | 0 | 0.0041 | 0 | 0.0114 | 0.0188 | 0.0024 | 0 | 0.0016 | 0.0025 | 0.0009 | 0.0025 | 0.0078 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0135 | 0.0303 | 0.0182 | 0.0276 | 0.0101 | 0.0545 | 0.0192 | 0.0267 | 0.0157 | 0.0079 | 0.0123 | 0.0385 | 0.0257 | 0.0212 | 0.021 | 0.0095 | 0.0441 | 0.026 |
Cytoplasmic Foci | 0.0097 | 0.0056 | 0.0103 | 0.0022 | 0.003 | 0.0067 | 0.0058 | 0.0026 | 0.0038 | 0.0012 | 0.0054 | 0.0037 | 0.0076 | 0.0017 | 0.0033 | 0.0015 | 0.0022 | 0.0017 |
Eisosomes | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0026 | 0.0007 | 0.0004 | 0.0004 | 0.0012 | 0.0006 | 0.0014 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.001 | 0.0004 | 0.0004 | 0.0003 | 0.0035 | 0.0004 |
Endosome | 0.0144 | 0.0024 | 0.0026 | 0.0004 | 0.0067 | 0.002 | 0.0043 | 0.0003 | 0.0002 | 0 | 0.001 | 0.0005 | 0.0024 | 0.0001 | 0.0009 | 0.0001 | 0.0034 | 0.0008 |
Golgi | 0.0049 | 0.0001 | 0.0003 | 0 | 0.0054 | 0.0002 | 0.0012 | 0.0001 | 0.0001 | 0 | 0.0013 | 0.0005 | 0.0025 | 0 | 0.0023 | 0 | 0.0003 | 0.0016 |
Lipid Particles | 0.0061 | 0.0008 | 0.0009 | 0.0002 | 0.009 | 0.0005 | 0.002 | 0.0002 | 0.0002 | 0.0001 | 0.0023 | 0.0037 | 0.0044 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0016 |
Mitochondria | 0.0032 | 0.0005 | 0.0006 | 0.0001 | 0.0018 | 0.0002 | 0.0022 | 0.0002 | 0.0005 | 0.0001 | 0.0011 | 0.0005 | 0.0007 | 0.0006 | 0.0015 | 0.0006 | 0.0002 | 0.0048 |
None | 0.8426 | 0.937 | 0.9517 | 0.9677 | 0.9064 | 0.9181 | 0.8934 | 0.9422 | 0.9618 | 0.9875 | 0.9325 | 0.9101 | 0.9028 | 0.95 | 0.9378 | 0.9721 | 0.9295 | 0.9535 |
Nuclear Periphery | 0.0113 | 0.0013 | 0.0005 | 0.0003 | 0.0036 | 0.0047 | 0.0034 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0005 | 0.0059 | 0.0018 | 0.0003 | 0.0005 | 0.0054 | 0.0004 |
Nucleolus | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0006 | 0.0004 | 0.0019 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0005 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.006 | 0.0016 | 0.0005 | 0.0002 | 0.0007 | 0.0014 | 0.0069 | 0.0008 | 0.0012 | 0.0003 | 0.0002 | 0.0029 | 0.0071 | 0.0037 | 0.0011 | 0.0005 | 0.0018 | 0.0003 |
Peroxisomes | 0.0055 | 0.0004 | 0.0014 | 0.0001 | 0.0026 | 0.0002 | 0.0023 | 0.0001 | 0.0033 | 0.0001 | 0.0123 | 0.0014 | 0.0052 | 0.0001 | 0.0101 | 0.0002 | 0.0001 | 0.0013 |
Punctate Nuclear | 0.0068 | 0.0053 | 0.0016 | 0.0003 | 0.0029 | 0.0027 | 0.0076 | 0.0018 | 0.0038 | 0.0017 | 0.0004 | 0.0028 | 0.0026 | 0.0023 | 0.0017 | 0.0006 | 0.0028 | 0.0003 |
Vacuole | 0.0011 | 0.0006 | 0.0004 | 0.0001 | 0.0011 | 0.0003 | 0.0015 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0002 |
Vacuole Periphery | 0.001 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1942.4516 | 2020.2731 | 2342.569 | 2129.7063 | 2230.6745 | 1899.7343 | 1831.9984 | 1648.1957 | 1905.5228 | 1797.3788 |
Translational Efficiency | 1.144 | 1.1538 | 1.0609 | 1.1364 | 1.1862 | 1.2754 | 1.2293 | 1.3597 | 1.1707 | 1.3269 |
Dataset | Hit |
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Protein Abundance | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |