Standard name
Human Ortholog
Description Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.99 0.86 0.96 0.92 0.95 0.79 0.75 0.71 0.71 0.99 0.98 0.99 – 0.98 0.98 0.84 0.85 0.78 0.88 0.84 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.14 0.07 0.05 0.05 0.35 0.26 0.35 0.39 0 0 0 – 0 0 0.06 0.05 0.11 0 0 0
Nucleus 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 6 1 0 1 0
Bud 0 1 0 0 1 0 2 1 1 1 2 3 0 0 0 – 0 0 5 4 1 3 1 6
Bud Neck 3 3 2 3 0 4 17 15 1 4 4 2 0 2 0 – 1 2 2 1 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 0 1 0 0 1 0 0 0 0 0 0 0 0 – 7 5 1 6 1 0 1 0
Cytoplasm 456 525 140 91 18 191 174 226 130 122 101 119 145 299 263 – 302 301 404 439 114 171 134 179
Endoplasmic Reticulum 0 2 1 0 0 1 0 0 0 0 3 0 1 4 1 – 20 29 2 1 1 0 0 1
Endosome 2 0 0 0 0 0 0 0 0 0 0 0 0 2 0 – 1 1 7 3 2 1 2 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 2 4 1 2 0 1 2 0
Mitochondria 5 11 1 1 3 13 9 13 57 42 50 65 0 0 0 – 7 5 27 23 15 2 6 9
Nucleus 0 1 1 0 1 0 1 1 0 1 1 2 1 1 0 – 2 0 0 0 0 0 0 1
Nuclear Periphery 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0
SpindlePole 0 2 1 0 0 2 4 5 3 7 1 4 0 1 0 – 2 1 5 1 0 3 2 2
Vac/Vac Membrane 4 2 1 0 0 0 0 1 0 0 1 0 0 0 0 – 2 1 9 3 0 2 1 0
Unique Cell Count 465 543 145 92 21 200 190 238 164 162 142 168 146 304 265 309 308 480 519 147 196 160 209
Labelled Cell Count 472 550 148 96 23 211 208 262 193 178 164 195 147 309 265 346 349 480 519 147 196 160 209


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.4 4.5 4.5 3.4 3.8 4.1 4.2 3.2 3.6 3.2 3.3 4.7 4.7 4.5 4.5 5.6 5.5 5.0 5.5 5.4
Std Deviation (1e-4) 0.7 0.7 1.0 1.5 0.4 0.9 1.8 1.4 1.1 1.3 0.6 0.9 1.2 1.2 1.3 1.8 2.4 1.8 1.5 2.0 1.4
Intensity Change (Log2) – – – 0.01 -0.39 -0.24 -0.15 -0.1 -0.51 -0.32 -0.51 -0.46 0.07 0.07 -0.02 -0.01 0.3 0.3 0.16 0.29 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9372 -1.0643 -1.2502 -1.2636 -1.0752 -1.1221 4.4725 4.4869 4.7621 4.1428 4.6911 5.1434 1.8081 1.6881 1.4952 2.2907 1.7859 1.8901
Actin 0.0466 0.0012 0.0008 0.0001 0.0262 0.0055 0.014 0.0001 0.0006 0 0.01 0.0038 0.0286 0.0008 0.0049 0.0004 0.0042 0.0059
Bud 0.0027 0.0034 0.0059 0 0.0011 0.0002 0.0053 0.0025 0.0014 0.0002 0.0032 0.0089 0.0003 0.0083 0.003 0.0038 0.0004 0.0002
Bud Neck 0.0027 0.0002 0.0001 0 0.0093 0.0003 0.0049 0.0001 0.0001 0 0.0003 0.0051 0.0005 0.0001 0.0013 0.0001 0.0001 0.0002
Bud Periphery 0.006 0.0045 0.002 0 0.003 0.0011 0.0099 0.002 0.0029 0.0003 0.0151 0.0125 0.0003 0.0052 0.0008 0.0084 0.0008 0.0004
Bud Site 0.0116 0.0027 0.0011 0 0.0041 0 0.0114 0.0188 0.0024 0 0.0016 0.0025 0.0009 0.0025 0.0078 0.0001 0.0002 0.0001
Cell Periphery 0.0005 0.0002 0.0001 0 0.0004 0 0.0006 0.0002 0.0001 0 0.0002 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0
Cytoplasm 0.0135 0.0303 0.0182 0.0276 0.0101 0.0545 0.0192 0.0267 0.0157 0.0079 0.0123 0.0385 0.0257 0.0212 0.021 0.0095 0.0441 0.026
Cytoplasmic Foci 0.0097 0.0056 0.0103 0.0022 0.003 0.0067 0.0058 0.0026 0.0038 0.0012 0.0054 0.0037 0.0076 0.0017 0.0033 0.0015 0.0022 0.0017
Eisosomes 0.0007 0.0003 0.0002 0.0002 0.0004 0.0001 0.0003 0.0002 0.0003 0 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0026 0.0007 0.0004 0.0004 0.0012 0.0006 0.0014 0.0003 0.0002 0.0001 0.0002 0.0004 0.001 0.0004 0.0004 0.0003 0.0035 0.0004
Endosome 0.0144 0.0024 0.0026 0.0004 0.0067 0.002 0.0043 0.0003 0.0002 0 0.001 0.0005 0.0024 0.0001 0.0009 0.0001 0.0034 0.0008
Golgi 0.0049 0.0001 0.0003 0 0.0054 0.0002 0.0012 0.0001 0.0001 0 0.0013 0.0005 0.0025 0 0.0023 0 0.0003 0.0016
Lipid Particles 0.0061 0.0008 0.0009 0.0002 0.009 0.0005 0.002 0.0002 0.0002 0.0001 0.0023 0.0037 0.0044 0.0001 0.0006 0.0001 0.0003 0.0016
Mitochondria 0.0032 0.0005 0.0006 0.0001 0.0018 0.0002 0.0022 0.0002 0.0005 0.0001 0.0011 0.0005 0.0007 0.0006 0.0015 0.0006 0.0002 0.0048
None 0.8426 0.937 0.9517 0.9677 0.9064 0.9181 0.8934 0.9422 0.9618 0.9875 0.9325 0.9101 0.9028 0.95 0.9378 0.9721 0.9295 0.9535
Nuclear Periphery 0.0113 0.0013 0.0005 0.0003 0.0036 0.0047 0.0034 0.0002 0.0006 0.0001 0.0002 0.0005 0.0059 0.0018 0.0003 0.0005 0.0054 0.0004
Nucleolus 0.0005 0.0007 0.0002 0.0001 0.0006 0.0004 0.0019 0.0003 0.0006 0.0002 0.0001 0.0005 0.0005 0.0004 0.0005 0.0002 0.0001 0.0001
Nucleus 0.006 0.0016 0.0005 0.0002 0.0007 0.0014 0.0069 0.0008 0.0012 0.0003 0.0002 0.0029 0.0071 0.0037 0.0011 0.0005 0.0018 0.0003
Peroxisomes 0.0055 0.0004 0.0014 0.0001 0.0026 0.0002 0.0023 0.0001 0.0033 0.0001 0.0123 0.0014 0.0052 0.0001 0.0101 0.0002 0.0001 0.0013
Punctate Nuclear 0.0068 0.0053 0.0016 0.0003 0.0029 0.0027 0.0076 0.0018 0.0038 0.0017 0.0004 0.0028 0.0026 0.0023 0.0017 0.0006 0.0028 0.0003
Vacuole 0.0011 0.0006 0.0004 0.0001 0.0011 0.0003 0.0015 0.0003 0.0002 0.0001 0.0002 0.0006 0.0005 0.0003 0.0003 0.0005 0.0003 0.0002
Vacuole Periphery 0.001 0.0002 0.0001 0 0.0005 0.0002 0.0005 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1942.4516 2020.2731 2342.569 2129.7063 2230.6745 1899.7343 1831.9984 1648.1957 1905.5228 1797.3788
Translational Efficiency 1.144 1.1538 1.0609 1.1364 1.1862 1.2754 1.2293 1.3597 1.1707 1.3269

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘