Standard name
Human Ortholog
Description Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0.06 0 0 – 0 0 0.16 0.2 0.18 0.06 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.44 0.17 0.11 0 0.33 0.06 0.09 0.05 0 – 0 0 0.17 0.23 0.12 0.26 0.28 0.23 0.36 0.37 0.38 0.35 0.4 0.42
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.26 0.54 0.74 0.46 0.78 0.71 0.71 0.83 – 0.79 0.85 0.34 0.2 0.38 0.08 0.05 0.09 0 0 0.05 0.05 0.06 0.05
Nucleus 0.18 0.13 0.09 0.11 0.1 0.12 0.14 0.17 0.14 – 0.14 0.13 0.18 0.19 0.13 0.24 0.18 0.21 0.06 0.08 0 0.07 0 0
Nuclear Periphery 0.18 0.25 0.12 0.18 0 0.11 0.16 0.11 0.15 – 0.18 0.17 0.17 0.12 0.11 0.17 0.22 0.26 0.17 0.19 0.18 0.1 0.15 0.13
Nucleolus 0.34 0.21 0.13 0.08 0.13 0.2 0.14 0.12 0.19 – 0.21 0.19 0.2 0.13 0.15 0.3 0.29 0.29 0.08 0.08 0.06 0.08 0.05 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.23 0.08 0.18 0.05 0.15 0.06 0.08 0.08 0 – 0 0 0.07 0.1 0.07 0.16 0.18 0.09 0.21 0.14 0.19 0.2 0.16 0.17
Vac/Vac Membrane 0.12 0.21 0 0 0 0 0 0 0 – 0 0 0.07 0.09 0.12 0.09 0.14 0.09 0 0.05 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 1 1 2 1 1
Bud 1 1 2 4 3 5 8 21 12 – 6 9 0 1 2 2 0 1 1 2 1 4 2 5
Bud Neck 0 0 1 0 0 0 1 2 0 – 1 0 0 0 0 0 0 0 1 1 0 2 0 2
Bud Site 0 0 0 0 5 5 11 17 14 – 8 13 0 0 0 2 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Cytoplasm 5 9 10 13 13 11 33 18 11 – 6 8 28 45 51 21 20 30 0 1 0 2 2 7
Endoplasmic Reticulum 0 0 0 4 0 1 0 0 0 – 0 2 0 0 0 14 6 8 0 0 0 0 2 3
Endosome 71 38 25 11 99 22 44 28 14 – 3 6 30 51 34 96 54 62 84 114 121 85 105 131
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 1 0 1 0 2
Mitochondria 13 57 125 201 140 305 368 380 367 – 221 262 59 44 106 30 10 25 7 7 16 12 15 15
Nucleus 30 29 22 30 31 47 73 90 61 – 39 40 31 41 37 89 34 57 13 24 9 17 11 7
Nuclear Periphery 29 55 29 49 13 41 84 57 68 – 51 53 30 27 31 63 42 70 39 57 55 23 40 41
Nucleolus 56 47 30 22 39 76 71 63 86 – 58 59 34 28 42 109 56 77 18 24 18 19 14 13
Peroxisomes 1 0 3 2 19 2 12 5 0 – 0 0 0 0 1 1 3 0 3 5 8 4 4 5
SpindlePole 38 17 43 13 44 25 40 45 18 – 5 11 12 22 19 60 35 24 49 43 60 48 42 53
Vac/Vac Membrane 19 47 9 10 13 11 16 10 11 – 4 8 13 19 32 32 28 23 5 16 9 10 12 8
Unique Cell Count 163 220 233 270 303 389 516 534 443 278 307 174 221 278 365 194 269 233 309 318 247 267 311
Labelled Cell Count 263 300 299 359 419 551 761 737 662 402 471 237 278 355 520 289 379 233 309 318 247 267 311


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.9 3.9 4.0 4.3 3.9 4.0 4.0 3.5 – 3.5 3.5 4.5 4.9 4.6 6.4 7.3 7.1 5.2 5.2 5.6
Std Deviation (1e-4) 0.9 1.0 0.6 1.3 1.2 0.9 1.2 1.3 1.1 – 0.8 1.0 1.0 1.3 1.5 1.4 1.7 1.6 1.3 1.5 1.7
Intensity Change (Log2) – – – 0.03 0.12 0.0 0.02 0.02 -0.17 – -0.16 -0.18 0.19 0.31 0.21 0.71 0.9 0.84 0.4 0.39 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0 -1.0 1.1 -0.6 -1.3 – -1.4 -1.1 4.0 5.2 4.9 0.8 2.4 2.8
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 4.6 4.5 3.6
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 -12.4 -10.7 -10.8
Nucleus 0.6 0.3 1.0 1.8 2.7 1.6 – 1.6 1.3 2.5 2.8 1.4 4.6 2.5 3.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1.6 2.5 3.8
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 4.8 4.1 4.3
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 -0.6 -0.1 -3.1
Vacuole -0.1 0.2 -0.7 -0.5 -1.6 -1.0 – -1.7 -0.8 1.6 2.1 3.2 2.3 3.9 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9332 2.2723 1.9648 2.0469 2.5425 2.1931 3.2127 3.7927 3.3079 3.4607 2.972 3.2078 4.2209 4.0154 3.7233 3.1956 3.6316 3.5992
Actin 0.0187 0.0014 0.0103 0.0314 0.0717 0.0022 0.0107 0.0009 0.0063 0.0048 0.0036 0.0008 0.004 0.0037 0.0066 0.0007 0.004 0.0018
Bud 0.0003 0.0001 0.0002 0.0016 0.0016 0.0001 0.0002 0 0.0001 0.0006 0.0002 0 0.0001 0.0001 0.0002 0 0 0.0001
Bud Neck 0.0012 0.0005 0.0009 0.0024 0.0064 0.0017 0.0008 0.0004 0.0003 0.0012 0.0022 0.001 0.0008 0.0008 0.0005 0.0003 0.0004 0.0025
Bud Periphery 0.0006 0.0003 0.0003 0.0046 0.0032 0.0002 0.0003 0.0001 0.0001 0.0013 0.0002 0.0001 0.0003 0.0002 0.0008 0.0001 0.0001 0.0003
Bud Site 0.0009 0.0008 0.0006 0.0023 0.0131 0.0002 0.0016 0.0002 0.0002 0.0072 0.0033 0 0.0008 0.0008 0.0013 0.0003 0.0001 0.0003
Cell Periphery 0.0001 0.0001 0.0001 0.0004 0.0007 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.0195 0.0017 0.0031 0.0015 0.0023 0.001 0.0042 0.0017 0.0009 0.0062 0.0016 0.0003 0.0008 0.0021 0.0014 0.0003 0.011 0.0007
Cytoplasmic Foci 0.0116 0.0064 0.0118 0.0064 0.0106 0.0024 0.0101 0.0027 0.0046 0.0108 0.0173 0.001 0.0192 0.0044 0.0045 0.0035 0.0038 0.0041
Eisosomes 0.0002 0 0.0002 0.0002 0.0009 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0.0001
Endoplasmic Reticulum 0.0042 0.0024 0.0025 0.0038 0.0028 0.0007 0.0029 0.001 0.0014 0.0015 0.0013 0.0001 0.0011 0.0014 0.0018 0.0002 0.0028 0.0004
Endosome 0.0175 0.0076 0.015 0.0123 0.0221 0.0037 0.0211 0.0031 0.0055 0.0084 0.0314 0.0013 0.0101 0.0047 0.0065 0.0015 0.0073 0.0027
Golgi 0.0077 0.0026 0.0043 0.0066 0.0152 0.0021 0.0125 0.0014 0.0028 0.0024 0.012 0.0007 0.0086 0.0018 0.0028 0.001 0.0021 0.002
Lipid Particles 0.086 0.0462 0.0605 0.0624 0.069 0.0235 0.0373 0.0209 0.0267 0.0132 0.0395 0.0112 0.0935 0.0157 0.0309 0.009 0.0384 0.0285
Mitochondria 0.002 0.001 0.0022 0.0034 0.0092 0.0005 0.0047 0.0004 0.0018 0.0017 0.0021 0.0002 0.0023 0.0006 0.0101 0.0001 0.0012 0.0004
None 0.0019 0.0021 0.0051 0.0016 0.0023 0.0006 0.0091 0.0011 0.0038 0.003 0.0012 0.0003 0.0009 0.004 0.0178 0.0005 0.0055 0.0008
Nuclear Periphery 0.4845 0.4832 0.4567 0.2627 0.3229 0.487 0.5733 0.5361 0.5869 0.447 0.5146 0.4799 0.4219 0.3711 0.5775 0.382 0.6252 0.4582
Nucleolus 0.0245 0.034 0.0291 0.0573 0.052 0.0646 0.0132 0.0346 0.0149 0.021 0.0257 0.0646 0.0162 0.0319 0.0229 0.0484 0.0064 0.1142
Nucleus 0.0905 0.1297 0.0844 0.1124 0.0911 0.1064 0.0354 0.1304 0.0548 0.0722 0.0339 0.0968 0.065 0.0832 0.0574 0.0245 0.1111 0.1134
Peroxisomes 0.0059 0.0024 0.0089 0.0096 0.0079 0.001 0.0103 0.0009 0.0025 0.0028 0.0055 0.0005 0.0174 0.0022 0.0018 0.0008 0.0025 0.0036
Punctate Nuclear 0.2159 0.27 0.2931 0.4111 0.2901 0.2991 0.2436 0.2587 0.2812 0.3905 0.3008 0.3394 0.3308 0.4667 0.2436 0.5244 0.1729 0.2635
Vacuole 0.0015 0.0022 0.0037 0.0022 0.0022 0.0005 0.0025 0.0006 0.0005 0.0011 0.0012 0.0002 0.0009 0.0007 0.0023 0.0001 0.0007 0.0004
Vacuole Periphery 0.0046 0.0054 0.007 0.0035 0.0029 0.0025 0.006 0.0047 0.0045 0.0029 0.0023 0.0015 0.0051 0.0035 0.0087 0.0022 0.0044 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.895 6.3811 12.7098 12.0159 12.1775 11.2645 10.5267 10.3135 11.5447 13.7818
Translational Efficiency 1.1351 1.6113 0.6667 0.8492 0.7196 1.0541 0.938 0.9532 0.8462 0.8558

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
22 150 2089 175 852 1417 2264 167 874 1567 4353 342

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.97 824.13 854.52 966.10 708.10 793.02 893.10 930.75 707.54 796.00 874.59 948.84
Standard Deviation 53.70 110.06 104.36 123.49 85.85 104.45 119.17 113.22 85.26 105.40 113.95 119.90
Intensity Change Log 2 — 0.264726 0.316969 0.494027 — 0.163404 0.334869 0.394441 — 0.214150 0.326089 0.444303

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000130 0.000586 0.000499 0.000474 0.000429 0.000343 0.000486 0.000968 0.000422 0.000366 0.000492 0.000715
Bud Neck 0.000965 0.000852 0.004796 0.007014 0.001179 0.001456 0.004056 0.004657 0.001174 0.001398 0.004411 0.005863
Bud Site 0.000144 0.000373 0.002005 0.002747 0.001634 0.000543 0.001016 0.000993 0.001597 0.000527 0.001491 0.001891
Cell Periphery 0.000129 0.000059 0.000092 0.000064 0.000080 0.000051 0.000095 0.000059 0.000081 0.000051 0.000093 0.000061
Cytoplasm 0.061998 0.010453 0.026630 0.010343 0.013287 0.014990 0.035384 0.004998 0.014513 0.014556 0.031183 0.007733
Cytoplasmic Foci 0.240882* 0.015807 0.003239 0.000444 0.015632 0.012209 0.002231 0.000261 0.021302 0.012554 0.002715 0.000355
Eisosomes 0.000178 0.000059 0.000043 0.000016 0.000063 0.000041 0.000037 0.000020 0.000066 0.000043 0.000040 0.000018
Endoplasmic Reticulum 0.001183 0.003992 0.002859 0.004677 0.002331 0.002616 0.003102 0.006901 0.002302 0.002748 0.002985 0.005763
Endosome 0.018796 0.004031 0.002113 0.003360 0.004979 0.005679 0.001588 0.001510 0.005327 0.005521 0.001840 0.002456
Golgi 0.002344 0.002481 0.000330 0.000641 0.003247 0.002617 0.000234 0.000210 0.003224 0.002604 0.000280 0.000431
Lipid Particles 0.396314* 0.088465 0.003274 0.001377 0.138045* 0.059990 0.002722 0.002333 0.144546* 0.062715 0.002987 0.001844
Mitochondria 0.012919 0.007058 0.003983 0.002520 0.006951 0.005988 0.002679 0.001916 0.007102 0.006090 0.003305 0.002225
Mitotic Spindle 0.012210 0.224744* 0.198385* 0.618257* 0.103847* 0.207749* 0.241545* 0.330028* 0.101541* 0.209376* 0.220833* 0.477513*
None 0.008585 0.004495 0.015264 0.004692 0.010140 0.007306 0.010642 0.004734 0.010100 0.007037 0.012860 0.004712
Nuclear Periphery 0.022286 0.020659 0.019699 0.029643 0.014765 0.026519 0.025357 0.060558 0.014955 0.025958 0.022641 0.044739
Nuclear Periphery Foci 0.107122 0.420725* 0.202500* 0.175519 0.491794* 0.444251* 0.217426* 0.370513* 0.482111* 0.441999* 0.210263* 0.270736*
Nucleolus 0.055562 0.102200 0.173842 0.037244 0.115489 0.103296 0.107965 0.065561 0.113981 0.103191 0.139579 0.051071
Nucleus 0.035962 0.085897 0.337126* 0.079404 0.067817 0.098968 0.340275* 0.141333* 0.067015 0.097717 0.338764* 0.109644
Peroxisomes 0.014935 0.005807 0.001218 0.000551 0.006945 0.002969 0.000846 0.000410 0.007146 0.003240 0.001024 0.000482
Vacuole 0.005887 0.000990 0.001787 0.017300 0.001017 0.001690 0.002090 0.001858 0.001140 0.001623 0.001945 0.009760
Vacuole Periphery 0.001468 0.000267 0.000315 0.003714 0.000327 0.000729 0.000226 0.000178 0.000356 0.000684 0.000269 0.001988

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.80 -3.61 -5.52 -3.89 -4.12 -0.03 -5.10 -6.07 -6.17 -4.79 -0.41 -4.73 -7.76 -7.94 -6.27
Bud Neck -0.28 -12.03 -6.74 -6.73 -4.83 -1.47 -12.68 -8.21 -7.87 -4.84 -1.41 -15.35 -9.83 -9.53 -6.58
Bud Site -2.27 -4.84 -5.50 -5.19 -3.60 0.99 -0.41 -2.75 -4.81 -3.36 0.99 -0.96 -4.39 -6.68 -4.73
Cell Periphery 2.42 1.32 0.48 -2.34 -0.95 3.42 -0.67 -1.26 -3.17 -0.94 3.57 -0.73 -1.66 -4.23 -1.34
Cytoplasm 1.80 1.25 2.02 0.52 3.01 -1.07 -4.80 2.19 3.47 7.39 -0.74 -4.69 1.61 2.46 6.35
Cytoplasmic Foci 3.18 3.44 3.53 2.66 5.59 2.66 7.20 8.59 10.67 6.36 3.56 7.42 8.77 10.57 8.16
Eisosomes 3.60 4.52 4.21 1.04 -0.09 4.91 6.26 3.60 0.85 0.34 5.08 6.28 3.83 0.44 0.11
Endoplasmic Reticulum -4.68 -6.84 -5.79 -3.94 -4.46 -1.88 -8.16 -5.96 -5.71 -4.68 -2.65 -8.37 -7.91 -7.42 -6.44
Endosome 2.45 3.06 2.75 0.90 -2.52 -0.83 4.04 3.47 5.66 -0.66 -0.43 4.21 2.71 4.22 -2.54
Golgi -0.13 6.01 5.23 2.31 -0.76 1.77 8.28 8.11 5.15 -0.92 1.80 8.29 7.90 5.28 -1.04
Lipid Particles 3.33 4.61 4.64 5.59 4.30 8.69 16.92 17.02 15.66 1.69 8.92 17.34 17.53 16.64 4.16
Mitochondria 1.27 2.17 2.47 2.82 2.27 0.95 5.22 5.31 6.51 0.70 1.05 4.98 5.72 7.17 2.27
Mitotic Spindle -5.93 -7.24 -11.68 -5.97 -8.72 -9.76 -14.96 -6.14 -2.51 -0.75 -10.56 -15.18 -12.48 -7.67 -6.81
None 1.68 0.61 1.82 0.01 4.11 2.89 2.02 4.25 1.98 3.12 3.17 1.03 4.49 1.83 5.09
Nuclear Periphery -0.21 -0.16 -0.76 -0.94 -1.47 -5.54 -6.23 -3.67 -2.32 -2.38 -5.43 -5.63 -4.35 -2.13 -2.75
Nuclear Periphery Foci -3.79 0.32 1.35 9.01 3.21 3.13 21.33* 8.70 7.25 -1.95 2.76 22.05 14.88 14.07 0.34
Nucleolus 0.56 -2.63 1.95 3.10 16.70 1.53 -1.26 3.88 3.09 5.03 1.88 -4.76 7.41 6.64 13.87
Nucleus -2.45 -21.37 -6.76 -4.92 4.14 -4.45 -27.66 -8.77 -7.55 0.59 -4.48 -34.46 -10.28 -8.57 3.24
Peroxisomes 1.41 3.51 3.69 2.86 1.87 3.32 6.08 6.36 6.27 1.72 3.22 6.15 6.51 6.67 2.44
Vacuole 1.00 0.51 -3.63 -3.86 -3.77 -1.90 -4.83 -3.03 -2.70 -2.18 -1.84 -5.57 -4.54 -4.39 -4.18
Vacuole Periphery 1.84 1.91 -0.52 -1.11 -1.13 -1.40 0.95 -0.69 1.21 -2.53 -1.31 0.82 -1.13 -0.66 -1.22
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication
Localization
Cell Percentages nuclear periphery (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sir3

Sir3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sir3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available