Standard name
Description Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0.06 0 0 – 0 0 0.16 0.2 0.18 0.06 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.44 0.17 0.11 0 0.33 0.06 0.09 0.05 0 – 0 0 0.17 0.23 0.12 0.26 0.28 0.23 0.36 0.37 0.38 0.35 0.4 0.42
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.26 0.54 0.74 0.46 0.78 0.71 0.71 0.83 – 0.79 0.85 0.34 0.2 0.38 0.08 0.05 0.09 0 0 0.05 0.05 0.06 0.05
Nucleus 0.18 0.13 0.09 0.11 0.1 0.12 0.14 0.17 0.14 – 0.14 0.13 0.18 0.19 0.13 0.24 0.18 0.21 0.06 0.08 0 0.07 0 0
Nuclear Periphery 0.18 0.25 0.12 0.18 0 0.11 0.16 0.11 0.15 – 0.18 0.17 0.17 0.12 0.11 0.17 0.22 0.26 0.17 0.19 0.18 0.1 0.15 0.13
Nucleolus 0.34 0.21 0.13 0.08 0.13 0.2 0.14 0.12 0.19 – 0.21 0.19 0.2 0.13 0.15 0.3 0.29 0.29 0.08 0.08 0.06 0.08 0.05 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.23 0.08 0.18 0.05 0.15 0.06 0.08 0.08 0 – 0 0 0.07 0.1 0.07 0.16 0.18 0.09 0.21 0.14 0.19 0.2 0.16 0.17
Vac/Vac Membrane 0.12 0.21 0 0 0 0 0 0 0 – 0 0 0.07 0.09 0.12 0.09 0.14 0.09 0 0.05 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 1 1 2 1 1
Bud 1 1 2 4 3 5 8 21 12 – 6 9 0 1 2 2 0 1 1 2 1 4 2 5
Bud Neck 0 0 1 0 0 0 1 2 0 – 1 0 0 0 0 0 0 0 1 1 0 2 0 2
Bud Site 0 0 0 0 5 5 11 17 14 – 8 13 0 0 0 2 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Cytoplasm 5 9 10 13 13 11 33 18 11 – 6 8 28 45 51 21 20 30 0 1 0 2 2 7
Endoplasmic Reticulum 0 0 0 4 0 1 0 0 0 – 0 2 0 0 0 14 6 8 0 0 0 0 2 3
Endosome 71 38 25 11 99 22 44 28 14 – 3 6 30 51 34 96 54 62 84 114 121 85 105 131
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 1 0 1 0 2
Mitochondria 13 57 125 201 140 305 368 380 367 – 221 262 59 44 106 30 10 25 7 7 16 12 15 15
Nucleus 30 29 22 30 31 47 73 90 61 – 39 40 31 41 37 89 34 57 13 24 9 17 11 7
Nuclear Periphery 29 55 29 49 13 41 84 57 68 – 51 53 30 27 31 63 42 70 39 57 55 23 40 41
Nucleolus 56 47 30 22 39 76 71 63 86 – 58 59 34 28 42 109 56 77 18 24 18 19 14 13
Peroxisomes 1 0 3 2 19 2 12 5 0 – 0 0 0 0 1 1 3 0 3 5 8 4 4 5
SpindlePole 38 17 43 13 44 25 40 45 18 – 5 11 12 22 19 60 35 24 49 43 60 48 42 53
Vac/Vac Membrane 19 47 9 10 13 11 16 10 11 – 4 8 13 19 32 32 28 23 5 16 9 10 12 8
Unique Cell Count 163 220 233 270 303 389 516 534 443 278 307 174 221 278 365 194 269 233 309 318 247 267 311
Labelled Cell Count 263 300 299 359 419 551 761 737 662 402 471 237 278 355 520 289 379 233 309 318 247 267 311


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.9 3.9 4.0 4.3 3.9 4.0 4.0 3.5 – 3.5 3.5 4.5 4.9 4.6 6.4 7.3 7.1 5.2 5.2 5.6
Std Deviation (1e-4) 0.9 1.0 0.6 1.3 1.2 0.9 1.2 1.3 1.1 – 0.8 1.0 1.0 1.3 1.5 1.4 1.7 1.6 1.3 1.5 1.7
Intensity Change (Log2) – – – 0.03 0.12 0.0 0.02 0.02 -0.17 – -0.16 -0.18 0.19 0.31 0.21 0.71 0.9 0.84 0.4 0.39 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0 -1.0 1.1 -0.6 -1.3 – -1.4 -1.1 4.0 5.2 4.9 0.8 2.4 2.8
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 4.6 4.5 3.6
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 -12.4 -10.7 -10.8
Nucleus 0.6 0.3 1.0 1.8 2.7 1.6 – 1.6 1.3 2.5 2.8 1.4 4.6 2.5 3.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1.6 2.5 3.8
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 4.8 4.1 4.3
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 -0.6 -0.1 -3.1
Vacuole -0.1 0.2 -0.7 -0.5 -1.6 -1.0 – -1.7 -0.8 1.6 2.1 3.2 2.3 3.9 2.1