Standard name
Human Ortholog
Description Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0.06 0 0 – 0 0 0.16 0.2 0.18 0.06 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.44 0.17 0.11 0 0.33 0.06 0.09 0.05 0 – 0 0 0.17 0.23 0.12 0.26 0.28 0.23 0.36 0.37 0.38 0.35 0.4 0.42
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.26 0.54 0.74 0.46 0.78 0.71 0.71 0.83 – 0.79 0.85 0.34 0.2 0.38 0.08 0.05 0.09 0 0 0.05 0.05 0.06 0.05
Nucleus 0.18 0.13 0.09 0.11 0.1 0.12 0.14 0.17 0.14 – 0.14 0.13 0.18 0.19 0.13 0.24 0.18 0.21 0.06 0.08 0 0.07 0 0
Nuclear Periphery 0.18 0.25 0.12 0.18 0 0.11 0.16 0.11 0.15 – 0.18 0.17 0.17 0.12 0.11 0.17 0.22 0.26 0.17 0.19 0.18 0.1 0.15 0.13
Nucleolus 0.34 0.21 0.13 0.08 0.13 0.2 0.14 0.12 0.19 – 0.21 0.19 0.2 0.13 0.15 0.3 0.29 0.29 0.08 0.08 0.06 0.08 0.05 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.23 0.08 0.18 0.05 0.15 0.06 0.08 0.08 0 – 0 0 0.07 0.1 0.07 0.16 0.18 0.09 0.21 0.14 0.19 0.2 0.16 0.17
Vac/Vac Membrane 0.12 0.21 0 0 0 0 0 0 0 – 0 0 0.07 0.09 0.12 0.09 0.14 0.09 0 0.05 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 1 1 2 1 1
Bud 1 1 2 4 3 5 8 21 12 – 6 9 0 1 2 2 0 1 1 2 1 4 2 5
Bud Neck 0 0 1 0 0 0 1 2 0 – 1 0 0 0 0 0 0 0 1 1 0 2 0 2
Bud Site 0 0 0 0 5 5 11 17 14 – 8 13 0 0 0 2 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Cytoplasm 5 9 10 13 13 11 33 18 11 – 6 8 28 45 51 21 20 30 0 1 0 2 2 7
Endoplasmic Reticulum 0 0 0 4 0 1 0 0 0 – 0 2 0 0 0 14 6 8 0 0 0 0 2 3
Endosome 71 38 25 11 99 22 44 28 14 – 3 6 30 51 34 96 54 62 84 114 121 85 105 131
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 1 0 1 0 2
Mitochondria 13 57 125 201 140 305 368 380 367 – 221 262 59 44 106 30 10 25 7 7 16 12 15 15
Nucleus 30 29 22 30 31 47 73 90 61 – 39 40 31 41 37 89 34 57 13 24 9 17 11 7
Nuclear Periphery 29 55 29 49 13 41 84 57 68 – 51 53 30 27 31 63 42 70 39 57 55 23 40 41
Nucleolus 56 47 30 22 39 76 71 63 86 – 58 59 34 28 42 109 56 77 18 24 18 19 14 13
Peroxisomes 1 0 3 2 19 2 12 5 0 – 0 0 0 0 1 1 3 0 3 5 8 4 4 5
SpindlePole 38 17 43 13 44 25 40 45 18 – 5 11 12 22 19 60 35 24 49 43 60 48 42 53
Vac/Vac Membrane 19 47 9 10 13 11 16 10 11 – 4 8 13 19 32 32 28 23 5 16 9 10 12 8
Unique Cell Count 163 220 233 270 303 389 516 534 443 278 307 174 221 278 365 194 269 233 309 318 247 267 311
Labelled Cell Count 263 300 299 359 419 551 761 737 662 402 471 237 278 355 520 289 379 233 309 318 247 267 311


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.9 3.9 4.0 4.3 3.9 4.0 4.0 3.5 – 3.5 3.5 4.5 4.9 4.6 6.4 7.3 7.1 5.2 5.2 5.6
Std Deviation (1e-4) 0.9 1.0 0.6 1.3 1.2 0.9 1.2 1.3 1.1 – 0.8 1.0 1.0 1.3 1.5 1.4 1.7 1.6 1.3 1.5 1.7
Intensity Change (Log2) – – – 0.03 0.12 0.0 0.02 0.02 -0.17 – -0.16 -0.18 0.19 0.31 0.21 0.71 0.9 0.84 0.4 0.39 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0 -1.0 1.1 -0.6 -1.3 – -1.4 -1.1 4.0 5.2 4.9 0.8 2.4 2.8
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 4.6 4.5 3.6
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 -12.4 -10.7 -10.8
Nucleus 0.6 0.3 1.0 1.8 2.7 1.6 – 1.6 1.3 2.5 2.8 1.4 4.6 2.5 3.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1.6 2.5 3.8
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 4.8 4.1 4.3
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 -0.6 -0.1 -3.1
Vacuole -0.1 0.2 -0.7 -0.5 -1.6 -1.0 – -1.7 -0.8 1.6 2.1 3.2 2.3 3.9 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9332 2.2723 1.9648 2.0469 2.5425 2.1931 3.2127 3.7927 3.3079 3.4607 2.972 3.2078 4.2209 4.0154 3.7233 3.1956 3.6316 3.5992
Actin 0.0187 0.0014 0.0103 0.0314 0.0717 0.0022 0.0107 0.0009 0.0063 0.0048 0.0036 0.0008 0.004 0.0037 0.0066 0.0007 0.004 0.0018
Bud 0.0003 0.0001 0.0002 0.0016 0.0016 0.0001 0.0002 0 0.0001 0.0006 0.0002 0 0.0001 0.0001 0.0002 0 0 0.0001
Bud Neck 0.0012 0.0005 0.0009 0.0024 0.0064 0.0017 0.0008 0.0004 0.0003 0.0012 0.0022 0.001 0.0008 0.0008 0.0005 0.0003 0.0004 0.0025
Bud Periphery 0.0006 0.0003 0.0003 0.0046 0.0032 0.0002 0.0003 0.0001 0.0001 0.0013 0.0002 0.0001 0.0003 0.0002 0.0008 0.0001 0.0001 0.0003
Bud Site 0.0009 0.0008 0.0006 0.0023 0.0131 0.0002 0.0016 0.0002 0.0002 0.0072 0.0033 0 0.0008 0.0008 0.0013 0.0003 0.0001 0.0003
Cell Periphery 0.0001 0.0001 0.0001 0.0004 0.0007 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.0195 0.0017 0.0031 0.0015 0.0023 0.001 0.0042 0.0017 0.0009 0.0062 0.0016 0.0003 0.0008 0.0021 0.0014 0.0003 0.011 0.0007
Cytoplasmic Foci 0.0116 0.0064 0.0118 0.0064 0.0106 0.0024 0.0101 0.0027 0.0046 0.0108 0.0173 0.001 0.0192 0.0044 0.0045 0.0035 0.0038 0.0041
Eisosomes 0.0002 0 0.0002 0.0002 0.0009 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0.0001
Endoplasmic Reticulum 0.0042 0.0024 0.0025 0.0038 0.0028 0.0007 0.0029 0.001 0.0014 0.0015 0.0013 0.0001 0.0011 0.0014 0.0018 0.0002 0.0028 0.0004
Endosome 0.0175 0.0076 0.015 0.0123 0.0221 0.0037 0.0211 0.0031 0.0055 0.0084 0.0314 0.0013 0.0101 0.0047 0.0065 0.0015 0.0073 0.0027
Golgi 0.0077 0.0026 0.0043 0.0066 0.0152 0.0021 0.0125 0.0014 0.0028 0.0024 0.012 0.0007 0.0086 0.0018 0.0028 0.001 0.0021 0.002
Lipid Particles 0.086 0.0462 0.0605 0.0624 0.069 0.0235 0.0373 0.0209 0.0267 0.0132 0.0395 0.0112 0.0935 0.0157 0.0309 0.009 0.0384 0.0285
Mitochondria 0.002 0.001 0.0022 0.0034 0.0092 0.0005 0.0047 0.0004 0.0018 0.0017 0.0021 0.0002 0.0023 0.0006 0.0101 0.0001 0.0012 0.0004
None 0.0019 0.0021 0.0051 0.0016 0.0023 0.0006 0.0091 0.0011 0.0038 0.003 0.0012 0.0003 0.0009 0.004 0.0178 0.0005 0.0055 0.0008
Nuclear Periphery 0.4845 0.4832 0.4567 0.2627 0.3229 0.487 0.5733 0.5361 0.5869 0.447 0.5146 0.4799 0.4219 0.3711 0.5775 0.382 0.6252 0.4582
Nucleolus 0.0245 0.034 0.0291 0.0573 0.052 0.0646 0.0132 0.0346 0.0149 0.021 0.0257 0.0646 0.0162 0.0319 0.0229 0.0484 0.0064 0.1142
Nucleus 0.0905 0.1297 0.0844 0.1124 0.0911 0.1064 0.0354 0.1304 0.0548 0.0722 0.0339 0.0968 0.065 0.0832 0.0574 0.0245 0.1111 0.1134
Peroxisomes 0.0059 0.0024 0.0089 0.0096 0.0079 0.001 0.0103 0.0009 0.0025 0.0028 0.0055 0.0005 0.0174 0.0022 0.0018 0.0008 0.0025 0.0036
Punctate Nuclear 0.2159 0.27 0.2931 0.4111 0.2901 0.2991 0.2436 0.2587 0.2812 0.3905 0.3008 0.3394 0.3308 0.4667 0.2436 0.5244 0.1729 0.2635
Vacuole 0.0015 0.0022 0.0037 0.0022 0.0022 0.0005 0.0025 0.0006 0.0005 0.0011 0.0012 0.0002 0.0009 0.0007 0.0023 0.0001 0.0007 0.0004
Vacuole Periphery 0.0046 0.0054 0.007 0.0035 0.0029 0.0025 0.006 0.0047 0.0045 0.0029 0.0023 0.0015 0.0051 0.0035 0.0087 0.0022 0.0044 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.895 6.3811 12.7098 12.0159 12.1775 11.2645 10.5267 10.3135 11.5447 13.7818
Translational Efficiency 1.1351 1.6113 0.6667 0.8492 0.7196 1.0541 0.938 0.9532 0.8462 0.8558

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication
Localization
Cell Percentages nuclear periphery (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sir3

Sir3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sir3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available