Standard name | |
---|---|
Human Ortholog | |
Description | Peptidyl-prolyl cis-trans isomerase (PPIase); nuclear proline isomerase; affects expression of multiple genes via its role in nucleosome assembly; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; contains a nucleoplasmin-like fold and can form pentamers |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 1 | 2 | 8 | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 5 | 2 |
Bud Neck | 0 | 0 | 0 | 3 | 0 | 5 | 5 | 2 | 1 | 3 | 2 | 5 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 1 | 0 | 5 | 6 | 19 | 12 | 19 | 16 | 16 | 31 | 0 | 0 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 1 | 6 | 4 | 5 | 8 | 6 | 5 | 9 | 4 | 7 | 2 | 22 | 50 | 52 | 29 | 12 | 5 | 0 | 0 | 0 | 0 | 4 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 1 | 2 | 6 | 7 | 2 | 6 | 8 | 1 | 2 | 3 | 1 | 6 | 3 | 2 | 0 | 0 | 7 | 0 | 3 | 7 | 12 | 16 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 |
Mitochondria | 39 | 26 | 95 | 31 | 45 | 102 | 132 | 64 | 88 | 118 | 73 | 101 | 42 | 35 | 43 | 32 | 37 | 12 | 0 | 0 | 4 | 2 | 16 | 23 |
Nucleus | 125 | 77 | 58 | 4 | 50 | 41 | 44 | 31 | 33 | 43 | 37 | 35 | 11 | 27 | 29 | 40 | 36 | 22 | 81 | 47 | 33 | 20 | 37 | 31 |
Nuclear Periphery | 1 | 0 | 3 | 1 | 2 | 2 | 5 | 3 | 5 | 4 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 7 | 0 | 5 | 0 | 3 | 4 |
Nucleolus | 191 | 119 | 329 | 197 | 307 | 340 | 432 | 398 | 357 | 459 | 326 | 437 | 246 | 421 | 337 | 255 | 204 | 207 | 188 | 112 | 314 | 134 | 320 | 292 |
Peroxisomes | 0 | 0 | 3 | 4 | 4 | 13 | 24 | 34 | 25 | 40 | 23 | 34 | 3 | 5 | 10 | 3 | 4 | 4 | 0 | 0 | 1 | 0 | 6 | 4 |
SpindlePole | 3 | 0 | 4 | 19 | 23 | 40 | 83 | 90 | 89 | 113 | 96 | 125 | 14 | 31 | 30 | 4 | 1 | 0 | 4 | 0 | 19 | 10 | 32 | 35 |
Vac/Vac Membrane | 1 | 3 | 4 | 1 | 4 | 2 | 6 | 4 | 3 | 2 | 2 | 1 | 3 | 3 | 2 | 2 | 0 | 1 | 15 | 7 | 8 | 10 | 27 | 31 |
Unique Cell Count | 298 | 163 | 389 | 208 | 372 | 417 | 550 | 506 | 453 | 579 | 409 | 544 | 282 | 512 | 439 | 324 | 247 | 228 | 308 | 170 | 397 | 194 | 483 | 461 |
Labelled Cell Count | 363 | 226 | 506 | 266 | 451 | 567 | 764 | 650 | 639 | 811 | 588 | 780 | 343 | 580 | 515 | 370 | 296 | 254 | 308 | 170 | 397 | 194 | 483 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.2 | 29.5 | 23.2 | 20.6 | 17.7 | 15.4 | 14.2 | 14.2 | 14.5 | 13.3 | 12.8 | 13.4 | 26.2 | 26.5 | 24.2 | 44.2 | 40.3 | 43.5 | 26.4 | 27.6 | 29.1 |
Std Deviation (1e-4) | 5.5 | 7.8 | 6.7 | 6.8 | 5.0 | 5.7 | 5.3 | 5.4 | 8.2 | 6.0 | 5.1 | 6.3 | 13.2 | 13.9 | 14.9 | 22.9 | 15.6 | 16.2 | 9.3 | 8.9 | 10.4 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.39 | -0.6 | -0.71 | -0.71 | -0.68 | -0.8 | -0.86 | -0.79 | 0.17 | 0.19 | 0.06 | 0.93 | 0.79 | 0.9 | 0.18 | 0.25 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 3.3 | 2.6 | 3.7 | 2.9 | 3.6 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.2 | 0.4 | 0 | 0 | 0.5 | 0 | 0.2 | 0 | 4.0 | 5.1 | 5.8 | 4.6 | 2.5 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.7 | -4.4 | 0 | -0.1 | -4.6 | -1.8 | -1.5 | -2.3 | -2.2 | -3.0 | -7.4 | -5.6 | -5.1 | -2.9 | -6.1 |
Nucleus | -5.0 | -0.6 | -2.2 | -3.4 | -4.4 | -3.6 | -3.7 | -2.6 | -4.3 | -4.6 | -4.9 | -3.9 | -1.0 | -0.1 | -1.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.6 | -0.8 | -1.1 | -2.3 | -2.2 | -2.1 | -2.1 | -1.8 | -1.7 | 1.0 | -0.9 | -2.8 | -2.0 | -0.7 | 2.2 |
Peroxisomes | 0 | 0 | 2.4 | 3.2 | 4.4 | 3.8 | 4.5 | 3.9 | 4.2 | 0 | 0 | 1.7 | 0 | 0 | 0 |
SpindlePole | 4.9 | 3.8 | 5.3 | 7.3 | 8.1 | 8.6 | 8.7 | 9.6 | 9.6 | 3.1 | 3.9 | 4.2 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 16.5159 | 17.7839 | 17.4329 | 15.873 | 14.6593 | 17.9454 | 20.357 | 26.9536 | 23.94 | 20.8406 | 18.0963 | 23.3091 | 23.5348 | 28.9081 | 24.1549 | 23.5865 | 22.8126 | 23.0115 |
Actin | 0.0062 | 0 | 0 | 0 | 0.0001 | 0 | 0.0099 | 0 | 0.0045 | 0.0366 | 0.0079 | 0.0022 | 0.0012 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0035 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 |
Bud Site | 0.002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0054 | 0.0001 | 0.0002 | 0.0015 | 0.0009 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 |
Cytoplasm | 0.0007 | 0 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0086 | 0 | 0.0008 | 0.0008 | 0.0006 | 0.0017 | 0.0074 | 0 | 0.0063 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0055 | 0.0106 | 0.0011 | 0.0002 | 0.0157 | 0.002 | 0.0202 | 0.0006 | 0.0093 | 0.0132 | 0.0163 | 0.0016 | 0.0011 | 0 | 0.0023 | 0.0002 | 0.0007 | 0.002 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0088 | 0 | 0.0001 | 0.0023 | 0.0005 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0021 | 0 | 0.0019 | 0.0023 | 0.0004 | 0 | 0.0249 | 0 | 0.0017 | 0.0196 | 0.0325 | 0.002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0011 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0043 | 0 | 0.0044 | 0.0051 | 0.0132 | 0.0014 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Lipid Particles | 0.0074 | 0.0289 | 0.0013 | 0.0055 | 0.0197 | 0.0112 | 0.0176 | 0.0008 | 0.0063 | 0.017 | 0.0199 | 0.0034 | 0.0011 | 0.0001 | 0.0009 | 0.0004 | 0.0059 | 0.0106 |
Mitochondria | 0.0031 | 0.0001 | 0.0009 | 0.0007 | 0.0049 | 0.0018 | 0.0115 | 0.0002 | 0.0073 | 0.006 | 0.0525 | 0.0052 | 0.0084 | 0.0008 | 0.0004 | 0.0006 | 0.0028 | 0.003 |
None | 0.001 | 0 | 0.0008 | 0.0001 | 0.0006 | 0 | 0.0036 | 0 | 0.0008 | 0.0019 | 0.0008 | 0.001 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0056 | 0.0014 | 0.0159 | 0.0211 | 0.0267 | 0.0023 | 0.0248 | 0.0007 | 0.0036 | 0.0078 | 0.0029 | 0.004 | 0.0004 | 0.0001 | 0.0001 | 0.0061 | 0.0011 | 0.0006 |
Nucleolus | 0.8426 | 0.8508 | 0.9165 | 0.8043 | 0.7847 | 0.8991 | 0.7437 | 0.8664 | 0.8745 | 0.6992 | 0.7172 | 0.8681 | 0.8915 | 0.8856 | 0.9266 | 0.8484 | 0.9206 | 0.8893 |
Nucleus | 0.1107 | 0.0984 | 0.0573 | 0.1406 | 0.1064 | 0.0768 | 0.0766 | 0.1275 | 0.0802 | 0.1679 | 0.1188 | 0.0991 | 0.0836 | 0.1124 | 0.0618 | 0.1417 | 0.0634 | 0.0844 |
Peroxisomes | 0.0035 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0018 | 0 | 0.0017 | 0.0025 | 0.0025 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0056 | 0.009 | 0.0018 | 0.0021 | 0.025 | 0.0023 | 0.0253 | 0.0026 | 0.0017 | 0.005 | 0.002 | 0.0016 | 0.0024 | 0.0007 | 0.001 | 0.0019 | 0.0038 | 0.0069 |
Vacuole | 0.0009 | 0.0001 | 0.0006 | 0.008 | 0.0015 | 0.0007 | 0.0047 | 0.0002 | 0.0006 | 0.0038 | 0.0047 | 0.001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0007 | 0.0136 | 0.0124 | 0.003 | 0.0058 | 0.0005 | 0.002 | 0.0049 | 0.0048 | 0.0061 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.001 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 145.0617 | 174.8538 | 187.4843 | 134.1508 | 154.1033 | 170.2362 | 109.5449 | 105.3366 | 102.0721 | 100.9054 |
Translational Efficiency | 2.4756 | 2.1266 | 1.7336 | 2.5574 | 1.7948 | 1.456 | 1.7635 | 1.7637 | 1.529 | 1.8309 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1841 | 340 | 198 | 571 | 2246 | 1726 | 133 | 1148 | 4087 | 2066 | 331 | 1719 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1636.08 | 2049.68 | 2713.87 | 2968.93 | 1730.14 | 1962.76 | 2731.64 | 2952.83 | 1687.77 | 1977.06 | 2721.01 | 2958.18 |
Standard Deviation | 469.09 | 557.39 | 544.18 | 864.50 | 415.59 | 462.12 | 528.32 | 929.79 | 442.98 | 480.18 | 537.93 | 908.66 |
Intensity Change Log 2 | — | 0.325155 | 0.730108 | 0.859700 | — | 0.181995 | 0.658879 | 0.771210 | — | 0.253350 | 0.693938 | 0.814897 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000603 | 0.000463 | 0.000499 | 0.001227 | 0.000509 | 0.000638 | 0.000598 | 0.001000 | 0.000552 | 0.000609 | 0.000539 | 0.001076 |
Bud Neck | 0.000984 | 0.000833 | 0.002703 | 0.003919 | 0.000923 | 0.002539 | 0.004179 | 0.004653 | 0.000951 | 0.002259 | 0.003296 | 0.004409 |
Bud Site | 0.000211 | 0.000119 | 0.000540 | 0.000754 | 0.000163 | 0.000400 | 0.000724 | 0.001459 | 0.000184 | 0.000354 | 0.000614 | 0.001225 |
Cell Periphery | 0.000024 | 0.000011 | 0.000012 | 0.000056 | 0.000018 | 0.000054 | 0.000018 | 0.000255 | 0.000021 | 0.000047 | 0.000015 | 0.000189 |
Cytoplasm | 0.000124 | 0.000029 | 0.000022 | 0.000121 | 0.000060 | 0.000419 | 0.000033 | 0.000262 | 0.000089 | 0.000355 | 0.000026 | 0.000215 |
Cytoplasmic Foci | 0.006185 | 0.002120 | 0.009474 | 0.013533 | 0.003859 | 0.001894 | 0.009676 | 0.013858 | 0.004907 | 0.001931 | 0.009555 | 0.013750 |
Eisosomes | 0.000244 | 0.000121 | 0.000188 | 0.000206 | 0.000299 | 0.000167 | 0.000189 | 0.000226 | 0.000274 | 0.000159 | 0.000188 | 0.000219 |
Endoplasmic Reticulum | 0.000179 | 0.000078 | 0.000083 | 0.000136 | 0.000139 | 0.000197 | 0.000093 | 0.000170 | 0.000157 | 0.000177 | 0.000087 | 0.000159 |
Endosome | 0.000020 | 0.000010 | 0.000019 | 0.000787 | 0.000026 | 0.000063 | 0.000017 | 0.000784 | 0.000023 | 0.000054 | 0.000018 | 0.000785 |
Golgi | 0.000134 | 0.000074 | 0.000286 | 0.000521 | 0.000082 | 0.000093 | 0.000238 | 0.000423 | 0.000105 | 0.000090 | 0.000267 | 0.000455 |
Lipid Particles | 0.000091 | 0.000015 | 0.000329 | 0.000613 | 0.000030 | 0.000041 | 0.000013 | 0.000185 | 0.000057 | 0.000036 | 0.000202 | 0.000327 |
Mitochondria | 0.001425 | 0.001049 | 0.002407 | 0.005779 | 0.001107 | 0.001470 | 0.002393 | 0.006034 | 0.001250 | 0.001401 | 0.002401 | 0.005949 |
Mitotic Spindle | 0.004129 | 0.004408 | 0.018813 | 0.008118 | 0.002720 | 0.003162 | 0.003309 | 0.009279 | 0.003355 | 0.003367 | 0.012583 | 0.008893 |
None | 0.001138 | 0.000687 | 0.000316 | 0.000728 | 0.000448 | 0.000642 | 0.000396 | 0.000887 | 0.000759 | 0.000649 | 0.000348 | 0.000834 |
Nuclear Periphery | 0.000305 | 0.000177 | 0.000481 | 0.003367 | 0.000245 | 0.000336 | 0.001231 | 0.003254 | 0.000272 | 0.000310 | 0.000783 | 0.003292 |
Nuclear Periphery Foci | 0.000739 | 0.000181 | 0.000186 | 0.002192 | 0.000415 | 0.000375 | 0.000250 | 0.001777 | 0.000561 | 0.000343 | 0.000212 | 0.001915 |
Nucleolus | 0.619787* | 0.602893* | 0.699718* | 0.709980* | 0.655288* | 0.528559* | 0.688488* | 0.691419* | 0.639297* | 0.540792* | 0.695206* | 0.697584* |
Nucleus | 0.291176* | 0.344078* | 0.130772* | 0.173013* | 0.270847* | 0.438297* | 0.185780* | 0.190527* | 0.280004* | 0.422791* | 0.152875* | 0.184709* |
Peroxisomes | 0.070847 | 0.041070 | 0.131984* | 0.061394 | 0.060295 | 0.018585 | 0.101192* | 0.061275 | 0.065048 | 0.022285 | 0.119611* | 0.061314 |
Vacuole | 0.000464 | 0.000304 | 0.000482 | 0.002834 | 0.000638 | 0.000581 | 0.000426 | 0.002613 | 0.000560 | 0.000535 | 0.000459 | 0.002686 |
Vacuole Periphery | 0.001191 | 0.001281 | 0.000685 | 0.010723 | 0.001890 | 0.001489 | 0.000757 | 0.009661 | 0.001575 | 0.001455 | 0.000714 | 0.010014 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.58 | 1.94 | -6.34 | -7.62 | -6.85 | -3.91 | -1.24 | -10.74 | -6.89 | -4.04 | -2.25 | 0.03 | -11.73 | -9.28 | -8.68 |
Bud Neck | 1.68 | -5.32 | -8.45 | -8.90 | -2.48 | -8.05 | -3.98 | -11.51 | -5.61 | -0.59 | -7.77 | -6.12 | -14.04 | -7.20 | -2.49 |
Bud Site | 3.76 | -4.13 | -5.79 | -6.86 | -1.64 | -4.02 | -5.13 | -3.04 | -2.42 | -1.70 | -3.51 | -6.63 | -3.60 | -2.91 | -2.08 |
Cell Periphery | 4.86 | 4.37 | -2.90 | -4.03 | -3.89 | -2.15 | -0.15 | -1.57 | -1.28 | -1.56 | -1.95 | 1.97 | -1.67 | -1.33 | -1.72 |
Cytoplasm | 3.67 | 3.98 | -0.02 | -2.23 | -2.39 | -1.52 | 1.11 | -2.87 | 0.83 | -3.32 | -1.40 | 3.66 | -2.60 | 0.87 | -3.94 |
Cytoplasmic Foci | 4.28 | -0.89 | -2.75 | -4.39 | -0.72 | 2.38 | -1.29 | -5.67 | -7.04 | -0.82 | 4.43 | -1.59 | -6.00 | -8.20 | -1.12 |
Eisosomes | 3.40 | 1.32 | 0.96 | -3.82 | -0.54 | 9.04 | 5.47 | 3.66 | -3.51 | -1.64 | 6.31 | 3.73 | 2.61 | -4.40 | -1.55 |
Endoplasmic Reticulum | 6.78 | 5.19 | 1.01 | -1.38 | -1.14 | -1.92 | 1.62 | -0.62 | 0.54 | -1.33 | -0.96 | 4.45 | -0.07 | 0.52 | -1.78 |
Endosome | 2.56 | 0.16 | -1.39 | -1.41 | -1.40 | -1.79 | 0.58 | -1.68 | -1.59 | -1.70 | -2.04 | 0.57 | -2.16 | -2.06 | -2.18 |
Golgi | 3.47 | -5.25 | -1.85 | -2.15 | -1.09 | -0.88 | -4.51 | -4.17 | -3.97 | -2.03 | 0.96 | -7.37 | -3.98 | -4.11 | -2.03 |
Lipid Particles | 1.20 | -0.76 | -1.13 | -1.31 | -0.54 | -1.00 | 1.57 | -3.56 | -3.09 | -4.01 | 0.50 | -0.78 | -1.72 | -1.84 | -0.56 |
Mitochondria | 2.93 | -2.09 | -6.29 | -6.83 | -4.05 | -3.10 | -2.90 | -9.45 | -8.64 | -5.07 | -1.61 | -3.51 | -11.26 | -10.73 | -6.58 |
Mitotic Spindle | -0.06 | -2.58 | -1.64 | -1.04 | 1.78 | -0.43 | -0.51 | -3.46 | -2.90 | -2.73 | -0.03 | -2.67 | -3.66 | -3.16 | 1.11 |
None | 1.61 | 3.77 | 1.82 | -0.23 | -6.18 | -1.55 | 0.66 | -4.90 | -1.70 | -5.87 | 0.68 | 3.90 | -0.71 | -1.53 | -8.48 |
Nuclear Periphery | 2.76 | -1.09 | -4.20 | -4.38 | -3.89 | -0.93 | -0.92 | -3.62 | -3.47 | -1.53 | -0.49 | -1.16 | -4.99 | -4.87 | -3.40 |
Nuclear Periphery Foci | 2.99 | 3.02 | -2.29 | -3.41 | -3.41 | -0.09 | 0.97 | -3.17 | -3.07 | -3.38 | 1.36 | 3.12 | -3.75 | -4.26 | -4.88 |
Nucleolus | 0.99 | -4.92 | -7.41 | -5.66 | -0.83 | 13.89* | -1.20 | -3.58 | -14.99 | -0.27 | 12.50 | -3.95 | -7.63 | -16.89 | -0.43 |
Nucleus | -3.04 | 12.67 | 10.68 | 9.23 | -2.91 | -17.82 | 3.86 | 9.15 | 24.02 | -0.13 | -17.55 | 11.02 | 13.85 | 26.76 | -2.57 |
Peroxisomes | 5.94 | -6.54 | 1.55 | -3.42 | 6.90 | 17.33* | -3.92 | -0.62 | -13.50 | 3.64 | 20.59* | -7.79 | 0.82 | -14.26 | 7.87 |
Vacuole | 2.60 | -0.29 | -3.87 | -4.14 | -3.66 | 0.09 | 1.21 | -5.21 | -5.38 | -6.02 | -0.08 | 0.56 | -6.64 | -6.52 | -6.73 |
Vacuole Periphery | -0.33 | 2.24 | -6.43 | -6.39 | -6.84 | 0.76 | 2.30 | -5.68 | -6.34 | -6.98 | 0.35 | 2.97 | -8.26 | -8.61 | -9.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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