Standard name
Description Peptidyl-prolyl cis-trans isomerase (PPIase); nuclear proline isomerase; affects expression of multiple genes via its role in nucleosome assembly; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; contains a nucleoplasmin-like fold and can form pentamers
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.12 0.09 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.16 0.24 0.15 0.12 0.24 0.24 0.13 0.19 0.2 0.18 0.19 0.15 0.07 0.1 0.1 0.15 0.05 0 0 0 0 0 0.05
Nucleus 0.42 0.47 0.15 0 0.13 0.1 0.08 0.06 0.07 0.07 0.09 0.06 0 0.05 0.07 0.12 0.15 0.1 0.26 0.28 0.08 0.1 0.08 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.64 0.73 0.85 0.95 0.83 0.82 0.79 0.79 0.79 0.79 0.8 0.8 0.87 0.82 0.77 0.79 0.83 0.91 0.61 0.66 0.79 0.7 0.66 0.63
Peroxisomes 0 0 0 0 0 0 0 0.07 0.06 0.07 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.09 0.06 0.1 0.15 0.18 0.2 0.2 0.23 0.23 0.05 0.06 0.07 0 0 0 0 0 0.05 0.05 0.07 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 1 6 1 2 8 3 4 0 0 1 0 0 0 1 0 1 0 5 2
Bud Neck 0 0 0 3 0 5 5 2 1 3 2 5 1 2 3 0 0 0 0 0 1 0 1 3
Bud Site 0 0 1 0 5 6 19 12 19 16 16 31 0 0 3 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 3 1 6 4 5 8 6 5 9 4 7 2 22 50 52 29 12 5 0 0 0 0 4 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 0 0 0 0 0 0
Endosome 0 0 1 2 6 7 2 6 8 1 2 3 1 6 3 2 0 0 7 0 3 7 12 16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 5
Mitochondria 39 26 95 31 45 102 132 64 88 118 73 101 42 35 43 32 37 12 0 0 4 2 16 23
Nucleus 125 77 58 4 50 41 44 31 33 43 37 35 11 27 29 40 36 22 81 47 33 20 37 31
Nuclear Periphery 1 0 3 1 2 2 5 3 5 4 1 2 0 0 1 0 0 1 7 0 5 0 3 4
Nucleolus 191 119 329 197 307 340 432 398 357 459 326 437 246 421 337 255 204 207 188 112 314 134 320 292
Peroxisomes 0 0 3 4 4 13 24 34 25 40 23 34 3 5 10 3 4 4 0 0 1 0 6 4
SpindlePole 3 0 4 19 23 40 83 90 89 113 96 125 14 31 30 4 1 0 4 0 19 10 32 35
Vac/Vac Membrane 1 3 4 1 4 2 6 4 3 2 2 1 3 3 2 2 0 1 15 7 8 10 27 31
Unique Cell Count 298 163 389 208 372 417 550 506 453 579 409 544 282 512 439 324 247 228 308 170 397 194 483 461
Labelled Cell Count 363 226 506 266 451 567 764 650 639 811 588 780 343 580 515 370 296 254 308 170 397 194 483 461


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.2 29.5 23.2 20.6 17.7 15.4 14.2 14.2 14.5 13.3 12.8 13.4 26.2 26.5 24.2 44.2 40.3 43.5 26.4 27.6 29.1
Std Deviation (1e-4) 5.5 7.8 6.7 6.8 5.0 5.7 5.3 5.4 8.2 6.0 5.1 6.3 13.2 13.9 14.9 22.9 15.6 16.2 9.3 8.9 10.4
Intensity Change (Log2) – – – -0.17 -0.39 -0.6 -0.71 -0.71 -0.68 -0.8 -0.86 -0.79 0.17 0.19 0.06 0.93 0.79 0.9 0.18 0.25 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.3 2.6 3.7 2.9 3.6 4.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.2 0.4 0 0 0.5 0 0.2 0 4.0 5.1 5.8 4.6 2.5 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.7 -4.4 0 -0.1 -4.6 -1.8 -1.5 -2.3 -2.2 -3.0 -7.4 -5.6 -5.1 -2.9 -6.1
Nucleus -5.0 -0.6 -2.2 -3.4 -4.4 -3.6 -3.7 -2.6 -4.3 -4.6 -4.9 -3.9 -1.0 -0.1 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.6 -0.8 -1.1 -2.3 -2.2 -2.1 -2.1 -1.8 -1.7 1.0 -0.9 -2.8 -2.0 -0.7 2.2
Peroxisomes 0 0 2.4 3.2 4.4 3.8 4.5 3.9 4.2 0 0 1.7 0 0 0
SpindlePole 4.9 3.8 5.3 7.3 8.1 8.6 8.7 9.6 9.6 3.1 3.9 4.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.5159 17.7839 17.4329 15.873 14.6593 17.9454 20.357 26.9536 23.94 20.8406 18.0963 23.3091 23.5348 28.9081 24.1549 23.5865 22.8126 23.0115
Actin 0.0062 0 0 0 0.0001 0 0.0099 0 0.0045 0.0366 0.0079 0.0022 0.0012 0 0 0 0 0
Bud 0.0001 0 0 0 0.0001 0 0.0004 0.0001 0 0.0003 0.0002 0.0001 0.0001 0 0 0 0 0.0001
Bud Neck 0.0006 0 0.0001 0.0001 0.0002 0.0001 0.0006 0.0001 0.0001 0.0035 0.0002 0.0001 0.0001 0 0 0 0 0.0001
Bud Periphery 0.0001 0 0 0.0002 0.0002 0.0001 0.0007 0.0001 0 0.0004 0.0004 0.0001 0.0001 0 0 0 0 0.0002
Bud Site 0.002 0.0001 0.0001 0.0001 0.0001 0 0.0054 0.0001 0.0002 0.0015 0.0009 0.0001 0.0004 0 0 0 0 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0.0003 0.0004 0.0004 0.0006 0.0001 0.0002 0.0004 0.0007 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0003
Cytoplasm 0.0007 0 0.0007 0.0001 0.0002 0 0.0086 0 0.0008 0.0008 0.0006 0.0017 0.0074 0 0.0063 0 0 0
Cytoplasmic Foci 0.0055 0.0106 0.0011 0.0002 0.0157 0.002 0.0202 0.0006 0.0093 0.0132 0.0163 0.0016 0.0011 0 0.0023 0.0002 0.0007 0.002
Eisosomes 0.0001 0 0 0 0.0001 0 0.0002 0 0.0001 0.0003 0.0004 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0002 0 0.0001 0.0002 0 0 0.0088 0 0.0001 0.0023 0.0005 0.0003 0.0003 0 0 0 0 0
Endosome 0.0021 0 0.0019 0.0023 0.0004 0 0.0249 0 0.0017 0.0196 0.0325 0.002 0.0001 0 0 0 0 0
Golgi 0.0011 0.0001 0.0001 0.0003 0.0004 0.0001 0.0043 0 0.0044 0.0051 0.0132 0.0014 0.0001 0 0 0 0.0001 0.0001
Lipid Particles 0.0074 0.0289 0.0013 0.0055 0.0197 0.0112 0.0176 0.0008 0.0063 0.017 0.0199 0.0034 0.0011 0.0001 0.0009 0.0004 0.0059 0.0106
Mitochondria 0.0031 0.0001 0.0009 0.0007 0.0049 0.0018 0.0115 0.0002 0.0073 0.006 0.0525 0.0052 0.0084 0.0008 0.0004 0.0006 0.0028 0.003
None 0.001 0 0.0008 0.0001 0.0006 0 0.0036 0 0.0008 0.0019 0.0008 0.001 0.0007 0 0.0001 0 0 0.0001
Nuclear Periphery 0.0056 0.0014 0.0159 0.0211 0.0267 0.0023 0.0248 0.0007 0.0036 0.0078 0.0029 0.004 0.0004 0.0001 0.0001 0.0061 0.0011 0.0006
Nucleolus 0.8426 0.8508 0.9165 0.8043 0.7847 0.8991 0.7437 0.8664 0.8745 0.6992 0.7172 0.8681 0.8915 0.8856 0.9266 0.8484 0.9206 0.8893
Nucleus 0.1107 0.0984 0.0573 0.1406 0.1064 0.0768 0.0766 0.1275 0.0802 0.1679 0.1188 0.0991 0.0836 0.1124 0.0618 0.1417 0.0634 0.0844
Peroxisomes 0.0035 0.0002 0.0001 0.0001 0.0002 0.0001 0.0018 0 0.0017 0.0025 0.0025 0.0006 0.0001 0 0 0 0.0001 0.0001
Punctate Nuclear 0.0056 0.009 0.0018 0.0021 0.025 0.0023 0.0253 0.0026 0.0017 0.005 0.002 0.0016 0.0024 0.0007 0.001 0.0019 0.0038 0.0069
Vacuole 0.0009 0.0001 0.0006 0.008 0.0015 0.0007 0.0047 0.0002 0.0006 0.0038 0.0047 0.001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0006
Vacuole Periphery 0.0006 0.0002 0.0007 0.0136 0.0124 0.003 0.0058 0.0005 0.002 0.0049 0.0048 0.0061 0.0003 0.0001 0.0002 0.0002 0.001 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 145.0617 174.8538 187.4843 134.1508 154.1033 170.2362 109.5449 105.3366 102.0721 100.9054
Translational Efficiency 2.4756 2.1266 1.7336 2.5574 1.7948 1.456 1.7635 1.7637 1.529 1.8309

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘