Standard name | |
---|---|
Human Ortholog | |
Description | HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg2 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 9 | 13 | 20 | 8 | 14 | 14 | 6 | 8 | 2 | 15 | 0 | 7 | 30 | 30 | 18 | 13 | 0 | 0 | 0 | 0 | 0 | 5 |
Endoplasmic Reticulum | 2 | 1 | 2 | 0 | 3 | 3 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 7 | 7 | 3 | 0 | 1 | 2 | 1 | 1 | 5 |
Endosome | 4 | 0 | 3 | 2 | 11 | 2 | 4 | 4 | 2 | 0 | 3 | 0 | 6 | 3 | 9 | 11 | 5 | 3 | 0 | 4 | 2 | 1 | 3 | 10 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Mitochondria | 3 | 15 | 18 | 18 | 13 | 31 | 33 | 31 | 37 | 51 | 24 | 36 | 6 | 0 | 0 | 22 | 15 | 12 | 1 | 2 | 2 | 5 | 4 | 13 |
Nucleus | 16 | 9 | 6 | 11 | 24 | 14 | 42 | 60 | 53 | 73 | 35 | 54 | 3 | 8 | 11 | 36 | 37 | 15 | 4 | 3 | 0 | 0 | 2 | 5 |
Nuclear Periphery | 200 | 108 | 161 | 179 | 239 | 161 | 238 | 215 | 138 | 208 | 105 | 161 | 13 | 36 | 51 | 166 | 134 | 101 | 243 | 127 | 180 | 129 | 106 | 108 |
Nucleolus | 16 | 6 | 18 | 24 | 37 | 49 | 67 | 75 | 47 | 77 | 46 | 55 | 18 | 6 | 21 | 40 | 32 | 24 | 0 | 0 | 0 | 0 | 0 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 7 | 5 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 103 | 37 | 48 | 46 | 71 | 34 | 39 | 43 | 20 | 14 | 15 | 17 | 7 | 20 | 36 | 73 | 80 | 62 | 9 | 5 | 5 | 10 | 22 | 45 |
Unique Cell Count | 252 | 135 | 200 | 232 | 336 | 233 | 357 | 355 | 242 | 342 | 180 | 271 | 39 | 68 | 123 | 300 | 247 | 187 | 266 | 148 | 201 | 152 | 144 | 210 |
Labelled Cell Count | 346 | 178 | 265 | 293 | 420 | 303 | 441 | 449 | 304 | 438 | 233 | 340 | 57 | 82 | 168 | 396 | 331 | 234 | 266 | 148 | 201 | 152 | 144 | 210 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.1 | 8.3 | 9.2 | 9.1 | 9.8 | 9.6 | 9.1 | 9.2 | 8.6 | 8.4 | 8.7 | 8.7 | 7.5 | 8.8 | 8.9 | 10.4 | 11.5 | 14.8 | 9.9 | 10.9 | 11.5 |
Std Deviation (1e-4) | 1.6 | 1.6 | 2.3 | 2.1 | 2.7 | 2.8 | 2.7 | 3.2 | 2.5 | 3.2 | 2.8 | 3.0 | 1.3 | 1.5 | 2.0 | 3.2 | 3.9 | 4.1 | 1.9 | 2.1 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.08 | 0.06 | -0.02 | 0.0 | -0.1 | -0.13 | -0.08 | -0.09 | -0.31 | -0.07 | -0.04 | 0.17 | 0.32 | 0.68 | 0.1 | 0.24 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 0.7 | -0.6 | -0.3 | -0.3 | -1.2 | -1.4 | -2.0 | 0.5 | 0 | 0 | 5.3 | 2.2 | 1.2 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.5 | -2.5 | 1.4 | 0.1 | -0.1 | 2.0 | 2.0 | 1.3 | 1.4 | 0 | 0 | -3.4 | -0.7 | -1.2 | -0.9 |
Nucleus | 0.9 | 2.0 | 1.5 | 3.5 | 4.9 | 5.8 | 5.8 | 5.2 | 5.4 | 0 | 0 | 2.3 | 3.6 | 4.3 | 2.2 |
Nuclear Periphery | -0.8 | -2.4 | -2.7 | -3.5 | -4.8 | -5.3 | -4.7 | -4.7 | -4.9 | -6.1 | -4.4 | -7.2 | -5.8 | -5.8 | -5.6 |
Nucleolus | 0.5 | 0.7 | 3.5 | 3.1 | 3.7 | 3.1 | 4.0 | 4.3 | 3.3 | 5.9 | 0 | 2.2 | 1.5 | 1.3 | 1.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | -0.8 | -2.5 | -4.1 | -3.6 | -4.6 | -7.0 | -4.1 | -5.5 | -0.8 | 0.9 | 1.0 | 0.1 | 2.0 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.844 | 7.1792 | 6.3397 | 5.2856 | 5.5605 | 5.6892 | 6.8568 | 6.6244 | 6.9831 | 6.4829 | 6.4338 | 6.8237 | 6.1098 | 5.8929 | 6.6838 | 5.1042 | 5.6659 | 5.8559 |
Actin | 0.0247 | 0.0003 | 0.0045 | 0.0053 | 0.0004 | 0.0068 | 0.0201 | 0.0001 | 0.0085 | 0.0019 | 0.0009 | 0.0001 | 0.0001 | 0.0005 | 0.0007 | 0.0932 | 0.0002 | 0.0001 |
Bud | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0009 | 0.0003 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Bud Periphery | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0015 | 0.0001 | 0.0002 | 0.0018 | 0 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0.001 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0032 | 0.0002 | 0.0055 | 0.0057 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0017 | 0 | 0 | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0093 | 0 | 0.0036 | 0.0232 | 0.0037 | 0.0012 | 0.0122 | 0 | 0.0009 | 0.0062 | 0.005 | 0 | 0 | 0 | 0 | 0.0165 | 0 | 0 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0056 | 0.005 | 0.0056 | 0.0165 | 0.0052 | 0.0026 | 0.0039 | 0.0066 | 0.0098 | 0.0069 | 0.021 | 0.0012 | 0.002 | 0.0276 | 0.0267 | 0.011 | 0.0085 | 0.0043 |
Endosome | 0.0144 | 0.0009 | 0.0071 | 0.031 | 0.0022 | 0.0057 | 0.016 | 0.0005 | 0.0034 | 0.0125 | 0.0262 | 0.0003 | 0.0005 | 0.0016 | 0.0012 | 0.0015 | 0.0017 | 0.0005 |
Golgi | 0.0051 | 0.0001 | 0.0012 | 0.005 | 0.0009 | 0.0025 | 0.0086 | 0.0001 | 0.0008 | 0.0026 | 0.0023 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.004 | 0.0002 | 0.0001 |
Lipid Particles | 0.015 | 0.0008 | 0.0027 | 0.0495 | 0.0236 | 0.0106 | 0.0477 | 0.0005 | 0.0057 | 0.0123 | 0.0309 | 0.0016 | 0.0009 | 0.0006 | 0.0009 | 0.0077 | 0.0005 | 0.0006 |
Mitochondria | 0.0052 | 0.0046 | 0.0021 | 0.0059 | 0.0014 | 0.0009 | 0.0049 | 0.0011 | 0.0017 | 0.0015 | 0.0019 | 0.0002 | 0.0007 | 0.0034 | 0.0133 | 0.0017 | 0.0013 | 0.0013 |
None | 0.013 | 0.0001 | 0.0031 | 0.009 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0007 | 0.0005 | 0 | 0.0002 | 0.002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.8326 | 0.864 | 0.8685 | 0.7652 | 0.9137 | 0.9266 | 0.8157 | 0.9092 | 0.8891 | 0.8801 | 0.8331 | 0.9746 | 0.9338 | 0.8672 | 0.8427 | 0.7522 | 0.9264 | 0.9631 |
Nucleolus | 0.0016 | 0.0063 | 0.0003 | 0.0013 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0018 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Nucleus | 0.0239 | 0.0693 | 0.039 | 0.0172 | 0.0128 | 0.0164 | 0.0032 | 0.0139 | 0.0047 | 0.0028 | 0.0066 | 0.0021 | 0.0033 | 0.0202 | 0.0084 | 0.0026 | 0.0063 | 0.0046 |
Peroxisomes | 0.0062 | 0.0001 | 0.0021 | 0.0049 | 0.0006 | 0.0018 | 0.0188 | 0 | 0.0002 | 0.0031 | 0.0004 | 0 | 0 | 0 | 0 | 0.0512 | 0 | 0 |
Punctate Nuclear | 0.0065 | 0.0005 | 0.003 | 0.0122 | 0.0013 | 0.001 | 0.0043 | 0.0001 | 0.001 | 0.0087 | 0.0029 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0007 | 0.0002 | 0.0003 |
Vacuole | 0.0019 | 0.0022 | 0.0017 | 0.0023 | 0.001 | 0.0019 | 0.0014 | 0.0025 | 0.0025 | 0.001 | 0.0208 | 0.0004 | 0.0008 | 0.0029 | 0.0035 | 0.0014 | 0.0029 | 0.0008 |
Vacuole Periphery | 0.0285 | 0.0449 | 0.0494 | 0.043 | 0.0323 | 0.0199 | 0.0398 | 0.0645 | 0.0696 | 0.0588 | 0.042 | 0.0188 | 0.0569 | 0.0708 | 0.1008 | 0.0549 | 0.0515 | 0.0236 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.1617 | 36.7769 | 25.6573 | 30.5481 | 27.5819 | 50.6062 | 35.4371 | 34.8576 | 34.4312 | 35.033 |
Translational Efficiency | 0.7107 | 0.6277 | 0.7355 | 0.6387 | 0.6007 | 0.6351 | 0.599 | 0.476 | 0.5313 | 0.5442 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
179 | 1308 | 195 | 180 | 980 | 249 | 1978 | 1784 | 1159 | 1557 | 2173 | 1964 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1013.67 | 1029.24 | 1549.42 | 1181.30 | 756.60 | 1011.34 | 1099.96 | 1093.41 | 796.30 | 1026.38 | 1140.29 | 1101.47 |
Standard Deviation | 141.22 | 179.05 | 253.65 | 172.90 | 133.80 | 133.14 | 158.82 | 175.93 | 163.85 | 172.65 | 212.69 | 177.48 |
Intensity Change Log 2 | — | 0.021991 | 0.612140 | 0.220787 | — | 0.418665 | 0.539848 | 0.531232 | — | 0.205010 | 0.581685 | 0.361713 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000404 | 0.000306 | 0.000219 | 0.000150 | 0.000472 | 0.000175 | 0.000296 | 0.000217 | 0.000462 | 0.000285 | 0.000289 | 0.000211 |
Bud Neck | 0.001561 | 0.000265 | 0.001356 | 0.000784 | 0.000358 | 0.000099 | 0.001469 | 0.000744 | 0.000544 | 0.000239 | 0.001459 | 0.000748 |
Bud Site | 0.002418 | 0.000083 | 0.000119 | 0.000039 | 0.000247 | 0.000056 | 0.000170 | 0.000160 | 0.000582 | 0.000079 | 0.000165 | 0.000148 |
Cell Periphery | 0.000040 | 0.000068 | 0.000021 | 0.000014 | 0.000244 | 0.000025 | 0.000037 | 0.000042 | 0.000212 | 0.000061 | 0.000036 | 0.000040 |
Cytoplasm | 0.001761 | 0.000555 | 0.000050 | 0.000053 | 0.005209 | 0.000196 | 0.001463 | 0.000602 | 0.004677 | 0.000497 | 0.001336 | 0.000551 |
Cytoplasmic Foci | 0.005825 | 0.000496 | 0.001771 | 0.000356 | 0.002388 | 0.002588 | 0.000096 | 0.000769 | 0.002919 | 0.000831 | 0.000247 | 0.000731 |
Eisosomes | 0.000008 | 0.000005 | 0.000015 | 0.000003 | 0.000011 | 0.000004 | 0.000007 | 0.000005 | 0.000010 | 0.000005 | 0.000008 | 0.000005 |
Endoplasmic Reticulum | 0.006585 | 0.011297 | 0.002125 | 0.003356 | 0.048058 | 0.016739 | 0.007044 | 0.004191 | 0.041653 | 0.012167 | 0.006602 | 0.004115 |
Endosome | 0.009631 | 0.002085 | 0.000507 | 0.000602 | 0.005948 | 0.002793 | 0.001245 | 0.001995 | 0.006517 | 0.002198 | 0.001178 | 0.001867 |
Golgi | 0.007988 | 0.000238 | 0.000332 | 0.000118 | 0.000420 | 0.002794 | 0.000080 | 0.000252 | 0.001588 | 0.000647 | 0.000103 | 0.000240 |
Lipid Particles | 0.004246 | 0.002772 | 0.001024 | 0.000793 | 0.010342 | 0.003659 | 0.000389 | 0.001505 | 0.009401 | 0.002914 | 0.000446 | 0.001440 |
Mitochondria | 0.011696 | 0.003052 | 0.000826 | 0.000532 | 0.008389 | 0.005134 | 0.002526 | 0.001350 | 0.008900 | 0.003385 | 0.002373 | 0.001275 |
Mitotic Spindle | 0.031174 | 0.000891 | 0.003401 | 0.003581 | 0.000355 | 0.001121 | 0.004542 | 0.007086 | 0.005115 | 0.000928 | 0.004439 | 0.006765 |
None | 0.000184 | 0.001102 | 0.000131 | 0.000071 | 0.010345 | 0.000102 | 0.001147 | 0.000351 | 0.008776 | 0.000942 | 0.001056 | 0.000325 |
Nuclear Periphery | 0.258737* | 0.586710* | 0.607484* | 0.585755* | 0.463570* | 0.489006* | 0.551604* | 0.456748* | 0.431935* | 0.571085* | 0.556619* | 0.468571* |
Nuclear Periphery Foci | 0.624246* | 0.348090* | 0.316036* | 0.372529* | 0.377270* | 0.462663* | 0.277890* | 0.477438* | 0.415414* | 0.366413* | 0.281314* | 0.467823* |
Nucleolus | 0.003766 | 0.013319 | 0.042974 | 0.016053 | 0.020697 | 0.003639 | 0.047600 | 0.024678 | 0.018082 | 0.011771 | 0.047185 | 0.023887 |
Nucleus | 0.005201 | 0.017983 | 0.014434 | 0.011319 | 0.035499 | 0.004729 | 0.098054 | 0.016876 | 0.030819 | 0.015863 | 0.090550 | 0.016367 |
Peroxisomes | 0.000191 | 0.000417 | 0.001113 | 0.000188 | 0.000717 | 0.000370 | 0.000227 | 0.000253 | 0.000635 | 0.000409 | 0.000307 | 0.000247 |
Vacuole | 0.014435 | 0.005357 | 0.002892 | 0.001407 | 0.005548 | 0.002383 | 0.002263 | 0.002367 | 0.006920 | 0.004881 | 0.002319 | 0.002279 |
Vacuole Periphery | 0.009900 | 0.004909 | 0.003172 | 0.002297 | 0.003914 | 0.001724 | 0.001850 | 0.002372 | 0.004838 | 0.004400 | 0.001969 | 0.002365 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.64 | 0.92 | 1.14 | 3.74 | 1.82 | 3.88 | 2.87 | 4.71 | -0.74 | 3.00 | 2.72 | 2.54 | 3.96 | 2.19 | 3.21 |
Bud Neck | 2.75 | 0.98 | 1.93 | -2.55 | 1.86 | 2.33 | -5.68 | -2.57 | -7.45 | 4.39 | 2.78 | -4.37 | -0.59 | -6.18 | 4.63 |
Bud Site | 2.15 | 2.11 | 2.18 | 1.93 | 1.70 | 2.91 | 0.64 | 1.12 | -2.08 | 0.74 | 2.71 | 2.16 | 2.36 | -1.52 | 0.91 |
Cell Periphery | -1.57 | 2.29 | 2.99 | 4.77 | 1.60 | 6.35 | 6.03 | 5.76 | -1.77 | -0.52 | 5.05 | 6.08 | 5.82 | 1.75 | -0.43 |
Cytoplasm | 2.11 | 2.60 | 2.60 | 2.89 | 0.06 | 5.06 | 3.56 | 4.50 | -1.73 | 2.87 | 5.02 | 3.87 | 4.85 | -0.26 | 2.88 |
Cytoplasmic Foci | 2.29 | 1.71 | 2.37 | 0.65 | 4.13 | -0.06 | 2.94 | 1.99 | 1.02 | -2.47 | 2.54 | 3.54 | 2.77 | 0.25 | -1.93 |
Eisosomes | 1.84 | -0.91 | 2.56 | 2.90 | 3.63 | 4.00 | 2.85 | 4.58 | -0.87 | 2.20 | 5.39 | 2.35 | 5.01 | -0.40 | 3.30 |
Endoplasmic Reticulum | -2.91 | 4.14 | 2.32 | 5.65 | -1.15 | 6.26 | 13.23 | 14.13 | 3.16 | 4.63 | 10.47 | 13.16 | 14.07 | 7.31 | 4.38 |
Endosome | 3.86 | 4.62 | 4.59 | 4.18 | -0.55 | 2.19 | 6.61 | 5.11 | 0.60 | -1.91 | 5.87 | 7.92 | 6.43 | 0.69 | -1.93 |
Golgi | 1.93 | 1.91 | 1.97 | 1.45 | 5.07 | -1.21 | 2.85 | 1.10 | 1.30 | -1.62 | 1.30 | 2.37 | 2.13 | 1.25 | -1.41 |
Lipid Particles | 1.26 | 3.17 | 3.38 | 2.96 | 0.94 | 4.33 | 7.92 | 6.93 | 2.30 | -4.60 | 5.29 | 8.34 | 7.29 | 2.50 | -4.47 |
Mitochondria | 1.63 | 2.08 | 2.13 | 5.34 | 2.30 | 1.00 | 5.76 | 6.99 | 1.21 | 5.06 | 4.18 | 5.54 | 6.54 | 3.24 | 5.18 |
Mitotic Spindle | 3.67 | 3.37 | 3.40 | -2.19 | 0.16 | -1.62 | -4.60 | -4.85 | -4.12 | -1.08 | 3.24 | 0.71 | -0.15 | -4.54 | -1.06 |
None | -3.14 | 0.73 | 1.98 | 3.68 | 1.22 | 5.59 | 4.91 | 5.45 | -3.44 | 2.82 | 4.99 | 4.85 | 5.44 | 2.51 | 2.84 |
Nuclear Periphery | -15.13* | -12.76 | -11.67 | 0.04 | 0.72 | -1.34 | -7.34 | 0.63 | 1.81 | 9.75 | -12.86 | -11.27 | -3.55 | 11.42 | 9.50 |
Nuclear Periphery Foci | 10.12 | 10.20 | 7.65 | -1.27 | -2.43 | -4.54 | 9.84 | -9.50 | -0.80 | -23.73* | 4.38 | 13.22 | -5.28 | -11.70 | -23.43 |
Nucleolus | -4.63 | -4.41 | -2.62 | -0.58 | 2.81 | 6.15 | -7.61 | -1.27 | -8.45 | 6.80 | 2.54 | -8.97 | -2.02 | -5.04 | 7.31 |
Nucleus | -4.14 | -2.28 | -1.36 | 1.81 | 0.81 | 7.51 | -10.60 | 4.52 | -5.49 | 16.48 | 4.17 | -11.28 | 4.10 | -0.37 | 16.39 |
Peroxisomes | -1.41 | -3.56 | 0.79 | 1.94 | 3.87 | 2.06 | 4.23 | 3.99 | 0.85 | -0.55 | 1.53 | 3.31 | 4.00 | 1.64 | 1.67 |
Vacuole | 2.82 | 3.20 | 3.53 | 4.20 | 1.12 | 1.99 | 2.68 | 2.61 | -0.00 | -0.11 | 2.11 | 4.13 | 4.19 | 3.02 | 0.13 |
Vacuole Periphery | 1.36 | 1.86 | 2.12 | 3.03 | 0.99 | 2.39 | 3.59 | 2.45 | -0.79 | -1.32 | 0.44 | 3.90 | 3.20 | 3.01 | -1.08 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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