Standard name | |
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Description | HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg2 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 9 | 13 | 20 | 8 | 14 | 14 | 6 | 8 | 2 | 15 | 0 | 7 | 30 | 30 | 18 | 13 | 0 | 0 | 0 | 0 | 0 | 5 |
Endoplasmic Reticulum | 2 | 1 | 2 | 0 | 3 | 3 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 7 | 7 | 3 | 0 | 1 | 2 | 1 | 1 | 5 |
Endosome | 4 | 0 | 3 | 2 | 11 | 2 | 4 | 4 | 2 | 0 | 3 | 0 | 6 | 3 | 9 | 11 | 5 | 3 | 0 | 4 | 2 | 1 | 3 | 10 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Mitochondria | 3 | 15 | 18 | 18 | 13 | 31 | 33 | 31 | 37 | 51 | 24 | 36 | 6 | 0 | 0 | 22 | 15 | 12 | 1 | 2 | 2 | 5 | 4 | 13 |
Nucleus | 16 | 9 | 6 | 11 | 24 | 14 | 42 | 60 | 53 | 73 | 35 | 54 | 3 | 8 | 11 | 36 | 37 | 15 | 4 | 3 | 0 | 0 | 2 | 5 |
Nuclear Periphery | 200 | 108 | 161 | 179 | 239 | 161 | 238 | 215 | 138 | 208 | 105 | 161 | 13 | 36 | 51 | 166 | 134 | 101 | 243 | 127 | 180 | 129 | 106 | 108 |
Nucleolus | 16 | 6 | 18 | 24 | 37 | 49 | 67 | 75 | 47 | 77 | 46 | 55 | 18 | 6 | 21 | 40 | 32 | 24 | 0 | 0 | 0 | 0 | 0 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 7 | 5 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 103 | 37 | 48 | 46 | 71 | 34 | 39 | 43 | 20 | 14 | 15 | 17 | 7 | 20 | 36 | 73 | 80 | 62 | 9 | 5 | 5 | 10 | 22 | 45 |
Unique Cell Count | 252 | 135 | 200 | 232 | 336 | 233 | 357 | 355 | 242 | 342 | 180 | 271 | 39 | 68 | 123 | 300 | 247 | 187 | 266 | 148 | 201 | 152 | 144 | 210 |
Labelled Cell Count | 346 | 178 | 265 | 293 | 420 | 303 | 441 | 449 | 304 | 438 | 233 | 340 | 57 | 82 | 168 | 396 | 331 | 234 | 266 | 148 | 201 | 152 | 144 | 210 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 9.1 | 8.3 | 9.2 | 9.1 | 9.8 | 9.6 | 9.1 | 9.2 | 8.6 | 8.4 | 8.7 | 8.7 | 7.5 | 8.8 | 8.9 | 10.4 | 11.5 | 14.8 | 9.9 | 10.9 | 11.5 |
Std Deviation (1e-4) | 1.6 | 1.6 | 2.3 | 2.1 | 2.7 | 2.8 | 2.7 | 3.2 | 2.5 | 3.2 | 2.8 | 3.0 | 1.3 | 1.5 | 2.0 | 3.2 | 3.9 | 4.1 | 1.9 | 2.1 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.08 | 0.06 | -0.02 | 0.0 | -0.1 | -0.13 | -0.08 | -0.09 | -0.31 | -0.07 | -0.04 | 0.17 | 0.32 | 0.68 | 0.1 | 0.24 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 0.7 | -0.6 | -0.3 | -0.3 | -1.2 | -1.4 | -2.0 | 0.5 | 0 | 0 | 5.3 | 2.2 | 1.2 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.5 | -2.5 | 1.4 | 0.1 | -0.1 | 2.0 | 2.0 | 1.3 | 1.4 | 0 | 0 | -3.4 | -0.7 | -1.2 | -0.9 |
Nucleus | 0.9 | 2.0 | 1.5 | 3.5 | 4.9 | 5.8 | 5.8 | 5.2 | 5.4 | 0 | 0 | 2.3 | 3.6 | 4.3 | 2.2 |
Nuclear Periphery | -0.8 | -2.4 | -2.7 | -3.5 | -4.8 | -5.3 | -4.7 | -4.7 | -4.9 | -6.1 | -4.4 | -7.2 | -5.8 | -5.8 | -5.6 |
Nucleolus | 0.5 | 0.7 | 3.5 | 3.1 | 3.7 | 3.1 | 4.0 | 4.3 | 3.3 | 5.9 | 0 | 2.2 | 1.5 | 1.3 | 1.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | -0.8 | -2.5 | -4.1 | -3.6 | -4.6 | -7.0 | -4.1 | -5.5 | -0.8 | 0.9 | 1.0 | 0.1 | 2.0 | 2.0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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