Standard name | |
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Human Ortholog | |
Description | HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg2 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 9 | 13 | 20 | 8 | 14 | 14 | 6 | 8 | 2 | 15 | 0 | 7 | 30 | 30 | 18 | 13 | 0 | 0 | 0 | 0 | 0 | 5 |
Endoplasmic Reticulum | 2 | 1 | 2 | 0 | 3 | 3 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 7 | 7 | 3 | 0 | 1 | 2 | 1 | 1 | 5 |
Endosome | 4 | 0 | 3 | 2 | 11 | 2 | 4 | 4 | 2 | 0 | 3 | 0 | 6 | 3 | 9 | 11 | 5 | 3 | 0 | 4 | 2 | 1 | 3 | 10 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Mitochondria | 3 | 15 | 18 | 18 | 13 | 31 | 33 | 31 | 37 | 51 | 24 | 36 | 6 | 0 | 0 | 22 | 15 | 12 | 1 | 2 | 2 | 5 | 4 | 13 |
Nucleus | 16 | 9 | 6 | 11 | 24 | 14 | 42 | 60 | 53 | 73 | 35 | 54 | 3 | 8 | 11 | 36 | 37 | 15 | 4 | 3 | 0 | 0 | 2 | 5 |
Nuclear Periphery | 200 | 108 | 161 | 179 | 239 | 161 | 238 | 215 | 138 | 208 | 105 | 161 | 13 | 36 | 51 | 166 | 134 | 101 | 243 | 127 | 180 | 129 | 106 | 108 |
Nucleolus | 16 | 6 | 18 | 24 | 37 | 49 | 67 | 75 | 47 | 77 | 46 | 55 | 18 | 6 | 21 | 40 | 32 | 24 | 0 | 0 | 0 | 0 | 0 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 7 | 5 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 103 | 37 | 48 | 46 | 71 | 34 | 39 | 43 | 20 | 14 | 15 | 17 | 7 | 20 | 36 | 73 | 80 | 62 | 9 | 5 | 5 | 10 | 22 | 45 |
Unique Cell Count | 252 | 135 | 200 | 232 | 336 | 233 | 357 | 355 | 242 | 342 | 180 | 271 | 39 | 68 | 123 | 300 | 247 | 187 | 266 | 148 | 201 | 152 | 144 | 210 |
Labelled Cell Count | 346 | 178 | 265 | 293 | 420 | 303 | 441 | 449 | 304 | 438 | 233 | 340 | 57 | 82 | 168 | 396 | 331 | 234 | 266 | 148 | 201 | 152 | 144 | 210 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.1 | 8.3 | 9.2 | 9.1 | 9.8 | 9.6 | 9.1 | 9.2 | 8.6 | 8.4 | 8.7 | 8.7 | 7.5 | 8.8 | 8.9 | 10.4 | 11.5 | 14.8 | 9.9 | 10.9 | 11.5 |
Std Deviation (1e-4) | 1.6 | 1.6 | 2.3 | 2.1 | 2.7 | 2.8 | 2.7 | 3.2 | 2.5 | 3.2 | 2.8 | 3.0 | 1.3 | 1.5 | 2.0 | 3.2 | 3.9 | 4.1 | 1.9 | 2.1 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.08 | 0.06 | -0.02 | 0.0 | -0.1 | -0.13 | -0.08 | -0.09 | -0.31 | -0.07 | -0.04 | 0.17 | 0.32 | 0.68 | 0.1 | 0.24 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 0.7 | -0.6 | -0.3 | -0.3 | -1.2 | -1.4 | -2.0 | 0.5 | 0 | 0 | 5.3 | 2.2 | 1.2 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.5 | -2.5 | 1.4 | 0.1 | -0.1 | 2.0 | 2.0 | 1.3 | 1.4 | 0 | 0 | -3.4 | -0.7 | -1.2 | -0.9 |
Nucleus | 0.9 | 2.0 | 1.5 | 3.5 | 4.9 | 5.8 | 5.8 | 5.2 | 5.4 | 0 | 0 | 2.3 | 3.6 | 4.3 | 2.2 |
Nuclear Periphery | -0.8 | -2.4 | -2.7 | -3.5 | -4.8 | -5.3 | -4.7 | -4.7 | -4.9 | -6.1 | -4.4 | -7.2 | -5.8 | -5.8 | -5.6 |
Nucleolus | 0.5 | 0.7 | 3.5 | 3.1 | 3.7 | 3.1 | 4.0 | 4.3 | 3.3 | 5.9 | 0 | 2.2 | 1.5 | 1.3 | 1.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | -0.8 | -2.5 | -4.1 | -3.6 | -4.6 | -7.0 | -4.1 | -5.5 | -0.8 | 0.9 | 1.0 | 0.1 | 2.0 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.844 | 7.1792 | 6.3397 | 5.2856 | 5.5605 | 5.6892 | 6.8568 | 6.6244 | 6.9831 | 6.4829 | 6.4338 | 6.8237 | 6.1098 | 5.8929 | 6.6838 | 5.1042 | 5.6659 | 5.8559 |
Actin | 0.0247 | 0.0003 | 0.0045 | 0.0053 | 0.0004 | 0.0068 | 0.0201 | 0.0001 | 0.0085 | 0.0019 | 0.0009 | 0.0001 | 0.0001 | 0.0005 | 0.0007 | 0.0932 | 0.0002 | 0.0001 |
Bud | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0009 | 0.0003 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Bud Periphery | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0015 | 0.0001 | 0.0002 | 0.0018 | 0 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0.001 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0032 | 0.0002 | 0.0055 | 0.0057 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0017 | 0 | 0 | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0093 | 0 | 0.0036 | 0.0232 | 0.0037 | 0.0012 | 0.0122 | 0 | 0.0009 | 0.0062 | 0.005 | 0 | 0 | 0 | 0 | 0.0165 | 0 | 0 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0056 | 0.005 | 0.0056 | 0.0165 | 0.0052 | 0.0026 | 0.0039 | 0.0066 | 0.0098 | 0.0069 | 0.021 | 0.0012 | 0.002 | 0.0276 | 0.0267 | 0.011 | 0.0085 | 0.0043 |
Endosome | 0.0144 | 0.0009 | 0.0071 | 0.031 | 0.0022 | 0.0057 | 0.016 | 0.0005 | 0.0034 | 0.0125 | 0.0262 | 0.0003 | 0.0005 | 0.0016 | 0.0012 | 0.0015 | 0.0017 | 0.0005 |
Golgi | 0.0051 | 0.0001 | 0.0012 | 0.005 | 0.0009 | 0.0025 | 0.0086 | 0.0001 | 0.0008 | 0.0026 | 0.0023 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.004 | 0.0002 | 0.0001 |
Lipid Particles | 0.015 | 0.0008 | 0.0027 | 0.0495 | 0.0236 | 0.0106 | 0.0477 | 0.0005 | 0.0057 | 0.0123 | 0.0309 | 0.0016 | 0.0009 | 0.0006 | 0.0009 | 0.0077 | 0.0005 | 0.0006 |
Mitochondria | 0.0052 | 0.0046 | 0.0021 | 0.0059 | 0.0014 | 0.0009 | 0.0049 | 0.0011 | 0.0017 | 0.0015 | 0.0019 | 0.0002 | 0.0007 | 0.0034 | 0.0133 | 0.0017 | 0.0013 | 0.0013 |
None | 0.013 | 0.0001 | 0.0031 | 0.009 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0007 | 0.0005 | 0 | 0.0002 | 0.002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.8326 | 0.864 | 0.8685 | 0.7652 | 0.9137 | 0.9266 | 0.8157 | 0.9092 | 0.8891 | 0.8801 | 0.8331 | 0.9746 | 0.9338 | 0.8672 | 0.8427 | 0.7522 | 0.9264 | 0.9631 |
Nucleolus | 0.0016 | 0.0063 | 0.0003 | 0.0013 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0018 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Nucleus | 0.0239 | 0.0693 | 0.039 | 0.0172 | 0.0128 | 0.0164 | 0.0032 | 0.0139 | 0.0047 | 0.0028 | 0.0066 | 0.0021 | 0.0033 | 0.0202 | 0.0084 | 0.0026 | 0.0063 | 0.0046 |
Peroxisomes | 0.0062 | 0.0001 | 0.0021 | 0.0049 | 0.0006 | 0.0018 | 0.0188 | 0 | 0.0002 | 0.0031 | 0.0004 | 0 | 0 | 0 | 0 | 0.0512 | 0 | 0 |
Punctate Nuclear | 0.0065 | 0.0005 | 0.003 | 0.0122 | 0.0013 | 0.001 | 0.0043 | 0.0001 | 0.001 | 0.0087 | 0.0029 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0007 | 0.0002 | 0.0003 |
Vacuole | 0.0019 | 0.0022 | 0.0017 | 0.0023 | 0.001 | 0.0019 | 0.0014 | 0.0025 | 0.0025 | 0.001 | 0.0208 | 0.0004 | 0.0008 | 0.0029 | 0.0035 | 0.0014 | 0.0029 | 0.0008 |
Vacuole Periphery | 0.0285 | 0.0449 | 0.0494 | 0.043 | 0.0323 | 0.0199 | 0.0398 | 0.0645 | 0.0696 | 0.0588 | 0.042 | 0.0188 | 0.0569 | 0.0708 | 0.1008 | 0.0549 | 0.0515 | 0.0236 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.1617 | 36.7769 | 25.6573 | 30.5481 | 27.5819 | 50.6062 | 35.4371 | 34.8576 | 34.4312 | 35.033 |
Translational Efficiency | 0.7107 | 0.6277 | 0.7355 | 0.6387 | 0.6007 | 0.6351 | 0.599 | 0.476 | 0.5313 | 0.5442 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |