Standard name
Human Ortholog
Description HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg2 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0 0 0 0 0 0 0.06 0 0.1 0.24 0.1 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0 0.07 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0.09 0.08 0 0.13 0.09 0.09 0.15 0.15 0.13 0.13 0.15 0 0 0.07 0.06 0.06 0 0 0 0 0 0.06
Nucleus 0.06 0.07 0 0.05 0.07 0.06 0.12 0.17 0.22 0.21 0.19 0.2 0.08 0.12 0.09 0.12 0.15 0.08 0 0 0 0 0 0
Nuclear Periphery 0.79 0.8 0.81 0.77 0.71 0.69 0.67 0.61 0.57 0.61 0.58 0.59 0.33 0.53 0.41 0.55 0.54 0.54 0.92 0.86 0.9 0.85 0.74 0.52
Nucleolus 0.06 0 0.09 0.1 0.11 0.21 0.19 0.21 0.19 0.23 0.26 0.2 0.46 0.09 0.17 0.13 0.13 0.13 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.41 0.27 0.24 0.2 0.21 0.15 0.11 0.12 0.08 0 0.08 0.06 0.18 0.29 0.29 0.24 0.32 0.33 0 0 0 0.07 0.15 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 0 0
Bud 0 0 0 0 1 1 2 1 0 3 0 0 0 0 0 1 0 0 1 2 2 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 2 1 1 2 2 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 3 1 0 0 0 0 0 0 0 0
Cytoplasm 2 2 9 13 20 8 14 14 6 8 2 15 0 7 30 30 18 13 0 0 0 0 0 5
Endoplasmic Reticulum 2 1 2 0 3 3 0 3 0 2 0 0 0 1 0 7 7 3 0 1 2 1 1 5
Endosome 4 0 3 2 11 2 4 4 2 0 3 0 6 3 9 11 5 3 0 4 2 1 3 10
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 1
Mitochondria 3 15 18 18 13 31 33 31 37 51 24 36 6 0 0 22 15 12 1 2 2 5 4 13
Nucleus 16 9 6 11 24 14 42 60 53 73 35 54 3 8 11 36 37 15 4 3 0 0 2 5
Nuclear Periphery 200 108 161 179 239 161 238 215 138 208 105 161 13 36 51 166 134 101 243 127 180 129 106 108
Nucleolus 16 6 18 24 37 49 67 75 47 77 46 55 18 6 21 40 32 24 0 0 0 0 0 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 2 1 7 5 3 1 0 0 1 0 1 0
Vac/Vac Membrane 103 37 48 46 71 34 39 43 20 14 15 17 7 20 36 73 80 62 9 5 5 10 22 45
Unique Cell Count 252 135 200 232 336 233 357 355 242 342 180 271 39 68 123 300 247 187 266 148 201 152 144 210
Labelled Cell Count 346 178 265 293 420 303 441 449 304 438 233 340 57 82 168 396 331 234 266 148 201 152 144 210


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 8.3 9.2 9.1 9.8 9.6 9.1 9.2 8.6 8.4 8.7 8.7 7.5 8.8 8.9 10.4 11.5 14.8 9.9 10.9 11.5
Std Deviation (1e-4) 1.6 1.6 2.3 2.1 2.7 2.8 2.7 3.2 2.5 3.2 2.8 3.0 1.3 1.5 2.0 3.2 3.9 4.1 1.9 2.1 2.6
Intensity Change (Log2) – – – -0.03 0.08 0.06 -0.02 0.0 -0.1 -0.13 -0.08 -0.09 -0.31 -0.07 -0.04 0.17 0.32 0.68 0.1 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.7 -0.6 -0.3 -0.3 -1.2 -1.4 -2.0 0.5 0 0 5.3 2.2 1.2 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.2 0 0 0 0 0 0 0 0 0 0 1.4 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 -2.5 1.4 0.1 -0.1 2.0 2.0 1.3 1.4 0 0 -3.4 -0.7 -1.2 -0.9
Nucleus 0.9 2.0 1.5 3.5 4.9 5.8 5.8 5.2 5.4 0 0 2.3 3.6 4.3 2.2
Nuclear Periphery -0.8 -2.4 -2.7 -3.5 -4.8 -5.3 -4.7 -4.7 -4.9 -6.1 -4.4 -7.2 -5.8 -5.8 -5.6
Nucleolus 0.5 0.7 3.5 3.1 3.7 3.1 4.0 4.3 3.3 5.9 0 2.2 1.5 1.3 1.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.0 -0.8 -2.5 -4.1 -3.6 -4.6 -7.0 -4.1 -5.5 -0.8 0.9 1.0 0.1 2.0 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.844 7.1792 6.3397 5.2856 5.5605 5.6892 6.8568 6.6244 6.9831 6.4829 6.4338 6.8237 6.1098 5.8929 6.6838 5.1042 5.6659 5.8559
Actin 0.0247 0.0003 0.0045 0.0053 0.0004 0.0068 0.0201 0.0001 0.0085 0.0019 0.0009 0.0001 0.0001 0.0005 0.0007 0.0932 0.0002 0.0001
Bud 0.0002 0 0 0.0002 0 0 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0 0.0001 0 0
Bud Neck 0.0007 0.0001 0.0001 0.0002 0 0.0009 0.0003 0 0.0006 0 0.0002 0 0 0 0 0.0001 0 0.0001
Bud Periphery 0.0004 0.0002 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0 0 0.0004 0.0004 0.0002 0.0001 0.0001
Bud Site 0.0015 0.0001 0.0002 0.0018 0 0.0001 0.0006 0 0.0002 0 0.001 0 0 0 0 0.0005 0 0
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0032 0.0002 0.0055 0.0057 0.0001 0.0003 0.0006 0.0001 0.0003 0.0005 0.0017 0 0 0.0013 0.0001 0.0002 0.0001 0
Cytoplasmic Foci 0.0093 0 0.0036 0.0232 0.0037 0.0012 0.0122 0 0.0009 0.0062 0.005 0 0 0 0 0.0165 0 0
Eisosomes 0.0003 0 0.0001 0.0001 0 0 0.0002 0 0 0 0 0 0 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0056 0.005 0.0056 0.0165 0.0052 0.0026 0.0039 0.0066 0.0098 0.0069 0.021 0.0012 0.002 0.0276 0.0267 0.011 0.0085 0.0043
Endosome 0.0144 0.0009 0.0071 0.031 0.0022 0.0057 0.016 0.0005 0.0034 0.0125 0.0262 0.0003 0.0005 0.0016 0.0012 0.0015 0.0017 0.0005
Golgi 0.0051 0.0001 0.0012 0.005 0.0009 0.0025 0.0086 0.0001 0.0008 0.0026 0.0023 0.0002 0.0001 0.0002 0.0002 0.004 0.0002 0.0001
Lipid Particles 0.015 0.0008 0.0027 0.0495 0.0236 0.0106 0.0477 0.0005 0.0057 0.0123 0.0309 0.0016 0.0009 0.0006 0.0009 0.0077 0.0005 0.0006
Mitochondria 0.0052 0.0046 0.0021 0.0059 0.0014 0.0009 0.0049 0.0011 0.0017 0.0015 0.0019 0.0002 0.0007 0.0034 0.0133 0.0017 0.0013 0.0013
None 0.013 0.0001 0.0031 0.009 0.0002 0.0001 0.0009 0.0001 0.0003 0.0007 0.0005 0 0.0002 0.002 0.0004 0.0003 0.0001 0.0001
Nuclear Periphery 0.8326 0.864 0.8685 0.7652 0.9137 0.9266 0.8157 0.9092 0.8891 0.8801 0.8331 0.9746 0.9338 0.8672 0.8427 0.7522 0.9264 0.9631
Nucleolus 0.0016 0.0063 0.0003 0.0013 0.0005 0.0006 0.0004 0.0005 0.0004 0.0002 0.0018 0.0002 0.0002 0.0004 0.0002 0.0001 0.0001 0.0004
Nucleus 0.0239 0.0693 0.039 0.0172 0.0128 0.0164 0.0032 0.0139 0.0047 0.0028 0.0066 0.0021 0.0033 0.0202 0.0084 0.0026 0.0063 0.0046
Peroxisomes 0.0062 0.0001 0.0021 0.0049 0.0006 0.0018 0.0188 0 0.0002 0.0031 0.0004 0 0 0 0 0.0512 0 0
Punctate Nuclear 0.0065 0.0005 0.003 0.0122 0.0013 0.001 0.0043 0.0001 0.001 0.0087 0.0029 0.0003 0.0003 0.0007 0.0003 0.0007 0.0002 0.0003
Vacuole 0.0019 0.0022 0.0017 0.0023 0.001 0.0019 0.0014 0.0025 0.0025 0.001 0.0208 0.0004 0.0008 0.0029 0.0035 0.0014 0.0029 0.0008
Vacuole Periphery 0.0285 0.0449 0.0494 0.043 0.0323 0.0199 0.0398 0.0645 0.0696 0.0588 0.042 0.0188 0.0569 0.0708 0.1008 0.0549 0.0515 0.0236

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.1617 36.7769 25.6573 30.5481 27.5819 50.6062 35.4371 34.8576 34.4312 35.033
Translational Efficiency 0.7107 0.6277 0.7355 0.6387 0.6007 0.6351 0.599 0.476 0.5313 0.5442

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘