TheCellVision

LEU3

Standard name	LEU3 ORF YLR451W Aliases leucine-responsive transcriptional regulator LEU3
Human Ortholog
Description	Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation

DOWNLOAD

Scale images

LEU3

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	–	0.05	0	0.07	0	0	0	0	0	0	–	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	–	0	0	0	0.1	0	0	0	0	0	–	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Cytoplasm	0	0	0.09	0.09	0.14	0.27	0.17	–	0.15	0.17	0.19	0.12	0.09	0.16	0.16	0.17	0.14	–	0	0	0	0	0	0
Endoplasmic Reticulum	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0.05	0	–	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0.05
Golgi	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Mitochondria	0.07	0.07	0	0.09	0.06	0.37	0.4	–	0.81	0.73	0.74	0.68	0.18	0.05	0.05	0.11	0	–	0	0	0	0	0.05	0.07
Nucleus	0.89	0.84	0.87	0.88	0.75	0.52	0.57	–	0.46	0.31	0.32	0.54	0.8	0.77	0.7	0.72	0.77	–	0.78	0.77	0.7	0.57	0.42	0.36
Nuclear Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0.05	0	0	0.05	0.07	0.06
Peroxisomes	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
SpindlePole	0	0	0	0	0.05	0	0.07	–	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0.05	0.07
Vac/Vac Membrane	0	0.09	0	0	0.05	0	0	–	0.05	0.05	0	0.05	0	0.05	0.09	0.07	0.07	–	0	0.1	0.15	0.25	0.25	0.28

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	1	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	2	2	0	1	2	2
Bud	0	0	0	1	0	2	1	–	4	4	5	0	0	0	0	0	0	–	0	4	1	6	4	7
Bud Neck	0	0	2	0	3	0	2	–	0	1	1	0	0	0	0	1	0	–	0	1	0	0	2	5
Bud Site	0	0	0	1	0	0	3	–	2	4	1	4	0	0	0	2	0	–	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	1	–	0	0	0	0	0	0
Cytoplasm	7	16	14	8	22	18	31	–	12	21	14	5	25	41	46	33	22	–	2	3	5	2	4	5
Endoplasmic Reticulum	2	1	1	0	1	0	1	–	0	0	0	0	0	1	1	9	6	–	1	0	2	1	3	3
Endosome	1	3	0	1	2	0	0	–	0	0	0	0	1	2	6	4	1	–	5	4	2	1	11	15
Golgi	0	2	0	0	0	0	0	–	0	0	0	0	1	0	0	2	0	–	3	2	0	1	2	1
Mitochondria	15	27	3	8	9	25	74	–	66	88	55	28	50	14	15	21	6	–	4	6	4	2	15	23
Nucleus	198	326	133	77	121	35	105	–	37	38	24	22	222	203	200	142	121	–	187	334	137	117	120	125
Nuclear Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	1	0	0	–	0	1	1	0	2	2
Nucleolus	4	4	0	0	0	0	0	–	1	2	0	1	8	3	9	2	2	–	12	12	1	10	19	21
Peroxisomes	0	0	0	0	0	0	0	–	0	0	0	0	0	0	2	0	0	–	0	0	0	0	1	0
SpindlePole	1	4	2	1	8	0	12	–	3	1	2	0	0	1	1	4	1	–	5	8	4	5	14	24
Vac/Vac Membrane	10	34	5	0	8	1	8	–	4	6	3	2	9	12	27	14	11	–	9	44	28	50	73	99
Unique Cell Count	223	389	153	88	161	67	183		81	121	74	41	279	264	285	197	157		241	432	196	206	288	350
Labelled Cell Count	238	418	160	97	174	81	237		129	165	105	62	316	277	308	234	171		241	432	196	206	288	350

Yeast GFP Assignment

Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	5.2	5.2	5.4	5.1	5.5	4.4	4.1	–	3.8	3.6	3.3	3.8	4.7	5.2	4.8	6.9	7.4	–	4.4	4.7	4.8
Std Deviation (1e^-4)	1.1	0.9	1.3	1.8	1.9	2.0	1.1	–	1.6	1.6	0.8	1.3	1.4	4.9	1.3	1.6	1.9	–	1.2	1.7	1.5
Intensity Change (Log₂)	–	–	–	-0.09	0.02	-0.31	-0.41	–	-0.5	-0.59	-0.74	-0.51	-0.22	-0.06	-0.19	0.34	0.46	–	-0.3	-0.22	-0.18

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Bud	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Bud Neck	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Bud Site	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Cell Periphery	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Cytoplasm	0	1.3	3.4	2.1	–	1.3	2.0	2.1	0	-0.1	1.9	2.0	2.1	1.3	–
Endoplasmic Reticulum	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Endosome	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Golgi	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Mitochondria	0	1.7	0	0	–	0	0	0	0	0	1.7	0	3.2	0	–
Nucleus	0.1	-2.7	-5.6	-5.9	–	-6.7	-9.4	-8.3	-4.7	-1.9	-2.5	-3.9	-3.4	-2.3	–
Nuclear Periphery	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Nucleolus	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Peroxisomes	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
SpindlePole	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–
Vacuole	0	0.8	0	0.5	–	0	0	0	0	0	0.6	2.4	1.6	1.5	–

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	3.0247	3.301	3.0408	2.6727	2.8833	3.076	2.1086	1.7969	1.7716	1.3186	1.4115	1.6537	1.5676	1.5246	1.3743	1.0758	1.1203	1.2521
Actin	0.0293	0.0001	0.0164	0.0005	0.0132	0.0121	0.0496	0.0003	0.0286	0	0.0076	0.0088	0.0134	0	0.0022	0.0006	0.0037	0.0028
Bud	0.0019	0	0.0052	0.0001	0.0002	0.0001	0.0035	0.0007	0.001	0	0.0002	0.0007	0.0021	0.0005	0.0003	0.0001	0.0008	0.0001
Bud Neck	0.0016	0.0003	0.0032	0.0003	0.0009	0.0101	0.0008	0.0004	0.0012	0.0001	0.0015	0.002	0.0035	0.0002	0.0008	0.0006	0.0018	0.0021
Bud Periphery	0.0013	0	0.0021	0.0001	0.0006	0.0003	0.0035	0.0003	0.0025	0	0.0005	0.0008	0.0011	0.0001	0.0009	0.0002	0.0013	0.0001
Bud Site	0.0035	0.0002	0.0016	0.0001	0.0006	0.0006	0.0022	0.0008	0.0077	0	0.0006	0.0005	0.0015	0.0003	0.0007	0.0001	0.0045	0.0002
Cell Periphery	0.0003	0	0.0002	0.0001	0.0002	0.0001	0.0003	0	0.0007	0	0.0001	0.0001	0.0002	0	0.0001	0.0001	0.0003	0
Cytoplasm	0.006	0.0023	0.0033	0.0171	0.0042	0.0014	0.0094	0.0019	0.0011	0.0002	0.0007	0.0096	0.0056	0.0009	0.004	0.0103	0.0021	0.0012
Cytoplasmic Foci	0.007	0.0001	0.0078	0.0003	0.0083	0.005	0.0308	0.0016	0.0007	0	0.0056	0.007	0.0047	0.0009	0.0019	0.0004	0.0035	0.0013
Eisosomes	0.0003	0	0.0002	0	0.0005	0.0001	0.0003	0	0.0001	0	0.0001	0.0001	0.0004	0	0.0001	0	0.0001	0
Endoplasmic Reticulum	0.002	0.0002	0.0017	0.0026	0.0019	0.0005	0.0029	0.0002	0.0007	0	0.0011	0.0014	0.0014	0	0.0025	0.0086	0.0005	0.0007
Endosome	0.0154	0.0003	0.0069	0.0013	0.0635	0.0053	0.0207	0.0004	0.0021	0	0.0097	0.0105	0.0077	0	0.0116	0.0077	0.0042	0.0018
Golgi	0.0034	0	0.0028	0	0.0077	0.0017	0.0107	0	0.0016	0	0.0283	0.0063	0.0026	0	0.0014	0.0005	0.0011	0.0006
Lipid Particles	0.0149	0	0.0082	0.0003	0.0068	0.0039	0.0153	0.0001	0.0006	0	0.0108	0.0061	0.0095	0	0.0021	0.0014	0.0037	0.0016
Mitochondria	0.007	0.0003	0.0082	0.0019	0.0702	0.0005	0.0316	0.0013	0.0108	0.0002	0.0058	0.0063	0.0161	0.0003	0.0048	0.0318	0.0069	0.0006
None	0.0171	0.0006	0.0137	0.0117	0.0151	0.0241	0.0095	0.0009	0.0096	0	0.0003	0.001	0.0055	0.0019	0.0052	0.0012	0.001	0.0014
Nuclear Periphery	0.0149	0.0099	0.0153	0.048	0.0162	0.0056	0.0481	0.0209	0.0295	0.002	0.0447	0.0188	0.0244	0.009	0.031	0.0352	0.0317	0.0064
Nucleolus	0.0233	0.0214	0.0227	0.0325	0.016	0.0468	0.0184	0.0219	0.0273	0.0106	0.0441	0.033	0.0285	0.0159	0.0285	0.0266	0.0215	0.0406
Nucleus	0.822	0.9591	0.8615	0.8694	0.7258	0.8644	0.6995	0.9304	0.8592	0.9859	0.8131	0.8447	0.7455	0.8337	0.7717	0.793	0.7692	0.8162
Peroxisomes	0.0102	0	0.0029	0	0.0027	0.0045	0.0234	0.0001	0.0006	0	0.0035	0.0076	0.0032	0	0.0006	0.0001	0.0033	0.0015
Punctate Nuclear	0.0151	0.0047	0.0142	0.0117	0.0435	0.0111	0.0151	0.0168	0.0124	0.0007	0.0193	0.0326	0.1182	0.1358	0.1262	0.0781	0.136	0.1203
Vacuole	0.0025	0.0003	0.0009	0.0011	0.0011	0.0013	0.002	0.0005	0.0009	0.0001	0.0011	0.0011	0.0029	0.0001	0.0015	0.0019	0.0021	0.0003
Vacuole Periphery	0.0011	0.0001	0.0009	0.0008	0.001	0.0002	0.0023	0.0005	0.0009	0.0001	0.0012	0.0009	0.002	0.0001	0.0019	0.0015	0.0008	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	10.5738	11.1204	7.7017	11.1015	11.5589	14.505	11.1501	11.1302	13.1383	13.3594
Translational Efficiency	0.7148	0.6536	0.8413	0.5543	0.5698	0.4584	0.6096	0.6627	0.5011	0.455

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1878	1599	1668	1300	1618	1557	147	66	3496	3156	1815	1366

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	675.31	778.88	904.83	885.82	719.94	739.98	949.34	901.96	695.97	759.69	908.43	886.60
Standard Deviation	73.38	135.87	108.76	117.90	82.42	173.87	96.17	107.55	80.82	156.99	108.47	117.47
Intensity Change Log ₂	—	0.205851	0.422097	0.391464	—	0.039610	0.399048	0.325187	—	0.122465	0.410250	0.357646

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000032	0.000223	0.000686	0.001176	0.000069	0.000300	0.000979	0.002926	0.000049	0.000261	0.000710	0.001261
Bud Neck	0.002011	0.003417	0.012628	0.024587	0.002508	0.004968	0.003271	0.003317	0.002241	0.004182	0.011870	0.023559
Bud Site	0.000325	0.000724	0.007667	0.017969	0.000417	0.001785	0.001369	0.013350	0.000368	0.001248	0.007157	0.017746
Cell Periphery	0.000076	0.000083	0.000073	0.000186	0.000046	0.000134	0.000088	0.000249	0.000062	0.000108	0.000074	0.000189
Cytoplasm	0.088005	0.030352	0.008837	0.029830	0.033504	0.030889	0.013010	0.060346	0.062781	0.030617	0.009175	0.031304
Cytoplasmic Foci	0.006656	0.004796	0.000415	0.003795	0.003382	0.004912	0.000377	0.018319	0.005141	0.004853	0.000412	0.004496
Eisosomes	0.000016	0.000025	0.000024	0.000032	0.000017	0.000030	0.000065	0.000079	0.000016	0.000027	0.000027	0.000035
Endoplasmic Reticulum	0.000448	0.002401	0.006173	0.011880	0.001148	0.002990	0.016257	0.009308	0.000772	0.002692	0.006990	0.011756
Endosome	0.000517	0.000948	0.000363	0.005909	0.000331	0.001660	0.000554	0.006251	0.000431	0.001299	0.000379	0.005925
Golgi	0.000086	0.000782	0.000057	0.006954	0.000209	0.000642	0.000057	0.012572	0.000143	0.000713	0.000057	0.007225
Lipid Particles	0.000827	0.001664	0.000257	0.000453	0.001156	0.002379	0.000965	0.001080	0.000979	0.002017	0.000314	0.000483
Mitochondria	0.001813	0.003379	0.001579	0.007729	0.002143	0.005551	0.000821	0.003069	0.001966	0.004451	0.001517	0.007504
Mitotic Spindle	0.000589	0.005062	0.002074	0.025959	0.001695	0.005545	0.000116	0.001184	0.001101	0.005300	0.001915	0.024762
None	0.011269	0.016685	0.003239	0.006599	0.007474	0.010677	0.001747	0.011152	0.009513	0.013721	0.003118	0.006819
Nuclear Periphery	0.000805	0.002338	0.001070	0.003775	0.001680	0.002203	0.001129	0.001289	0.001210	0.002271	0.001075	0.003655
Nuclear Periphery Foci	0.000239	0.001482	0.000151	0.001080	0.000414	0.001090	0.000342	0.001092	0.000320	0.001288	0.000166	0.001081
Nucleolus	0.007647	0.016090	0.004997	0.003885	0.013905	0.018887	0.002643	0.002996	0.010543	0.017470	0.004806	0.003842
Nucleus	0.876550*	0.906686*	0.944431*	0.829398*	0.928131*	0.900070*	0.942366*	0.824912*	0.900422*	0.903422*	0.944264*	0.829181*
Peroxisomes	0.000464	0.001178	0.000170	0.000383	0.000536	0.001522	0.000269	0.001339	0.000497	0.001348	0.000178	0.000429
Vacuole	0.001517	0.001480	0.004931	0.015194	0.001055	0.003047	0.013436	0.022925	0.001303	0.002253	0.005620	0.015568
Vacuole Periphery	0.000109	0.000206	0.000179	0.003226	0.000181	0.000719	0.000141	0.002242	0.000142	0.000459	0.000176	0.003178

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-8.35	-13.87	-17.23	-14.20	-6.08	-6.80	-6.89	-2.26	-2.08	-1.20	-10.44	-15.03	-14.54	-12.11	-5.51
Bud Neck	-2.10	-12.22	-13.65	-12.05	-4.78	-2.92	-3.79	-3.80	-1.46	0.29	-3.63	-12.45	-13.69	-11.98	-4.94
Bud Site	-2.58	-4.63	-6.53	-6.25	-2.39	-2.84	-4.44	-2.22	-1.90	-1.66	-3.55	-4.78	-6.76	-6.12	-2.65
Cell Periphery	-0.50	0.16	-4.45	-6.33	-8.27	-5.18	-5.07	-3.82	-1.52	-2.02	-3.73	-1.37	-7.80	-4.00	-8.39
Cytoplasm	12.65	21.30	15.32	1.93	-8.61	0.54	3.25	-0.03	-0.15	-1.30	10.85	21.58	12.29	2.27	-8.31
Cytoplasmic Foci	2.16	7.66	5.75	4.05	-2.87	-2.33	5.86	-0.53	-0.19	-1.07	0.80	9.35	5.15	4.75	-3.03
Eisosomes	-3.05	-6.61	-13.35	-7.67	-8.51	-6.40	-7.20	-5.36	-4.69	-1.36	-6.15	-9.99	-15.29	-9.52	-7.10
Endoplasmic Reticulum	-9.04	-17.38	-18.58	-15.61	-8.98	-7.42	-6.96	-5.67	-5.14	-0.50	-11.72	-17.62	-18.97	-16.24	-8.28
Endosome	-1.66	0.53	-4.07	-3.18	-4.29	-3.64	-4.27	-2.32	-1.63	-1.89	-3.85	-0.99	-4.62	-2.95	-4.51
Golgi	-2.02	0.10	-2.44	-1.64	-2.45	-1.83	0.73	-1.01	-0.90	-1.03	-2.74	0.79	-2.54	-1.91	-2.61
Lipid Particles	-2.89	5.18	1.91	3.82	-6.06	-3.11	-0.34	-1.78	0.75	-1.48	-4.27	6.48	2.89	5.77	-5.46
Mitochondria	-1.54	-0.03	-2.82	-1.19	-3.52	-2.94	1.92	-0.25	2.17	-1.37	-3.20	0.45	-3.00	-0.21	-3.64
Mitotic Spindle	-3.14	-3.37	-7.40	-5.54	-6.04	-2.33	0.59	-1.76	-1.40	-1.83	-3.89	-2.71	-7.40	-5.82	-6.30
None	-2.36	8.26	5.39	5.70	-4.99	-2.49	6.80	0.67	1.78	-1.48	-3.20	9.48	5.06	6.97	-5.34
Nuclear Periphery	-2.97	-7.31	-13.83	-4.94	-11.22	-1.56	-0.39	-2.67	-2.10	-2.58	-3.41	-3.01	-12.51	-6.86	-11.49
Nuclear Periphery Foci	-3.60	0.27	-5.21	0.21	-6.72	-3.26	-1.36	-2.63	-2.43	-2.56	-4.75	0.60	-5.97	-1.77	-6.43
Nucleolus	-4.92	1.85	3.83	8.68	2.34	-2.75	8.64	6.65	9.22	-1.36	-5.48	5.38	7.87	13.08	2.14
Nucleus	-4.03	-11.09	4.90	8.17	14.35	4.90	1.27	3.94	3.10	3.33	-0.10	-8.30	9.29	9.10	14.56
Peroxisomes	-1.75	2.07	1.44	3.64	-3.38	-3.89	1.50	-0.76	0.67	-1.13	-3.74	2.97	1.71	6.18	-3.20
Vacuole	0.00	-6.20	-12.48	-12.53	-9.80	-3.45	-4.48	-3.83	-3.73	-2.00	-2.80	-7.85	-13.02	-12.59	-9.17
Vacuole Periphery	-2.37	-1.81	-2.54	-2.31	-2.39	-2.65	0.14	-1.13	-0.36	-1.14	-3.14	-0.98	-2.55	-1.78	-2.47