Standard name
Description Protein that binds to the Rap1p C-terminus; acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation; RIF2 has a paralog, ORC4, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.14 0.32 0.3 0.23 0.17 0.15 0.21 0.13 0.15 0.16 0.1 0.25 0.29 0.41 0.35 0.33 0.34 0.1 0.12 0.15 0.22 0.19 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.66 0.26 0.15 0.22 0.39 0.69 0.75 0.58 0.62 0.7 0.67 0.78 0 0 0 0 0.09 0 0 0 0.1 0.05 0.06 0.05
Nucleus 0.44 0.5 0.33 0.38 0.29 0.31 0.32 0.32 0.36 0.3 0.28 0.28 0.69 0.65 0.51 0.42 0.31 0.37 0.49 0.52 0.32 0.35 0.29 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.29 0.2 0.18 0.22 0.12 0.08 0.12 0.09 0.1 0.13 0.09 0.05 0.08 0.09 0.26 0.33 0.33 0.27 0.22 0.25 0.29 0.33 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0
Bud 0 2 1 4 7 16 12 17 13 13 12 11 0 0 0 0 2 0 0 7 10 3 5 6
Bud Neck 0 1 0 0 1 0 0 1 1 0 0 0 2 1 0 0 0 0 0 0 2 2 1 3
Bud Site 0 1 0 0 2 6 8 5 7 11 13 14 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 26 36 85 67 59 78 75 136 61 79 77 56 53 68 101 73 137 110 19 42 62 47 56 68
Endoplasmic Reticulum 1 2 2 0 1 0 0 1 0 0 0 1 1 1 0 10 19 22 0 0 3 0 0 3
Endosome 0 0 20 12 10 3 3 13 6 9 10 2 3 3 8 3 18 14 1 5 13 0 6 8
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 2 0 1 1
Mitochondria 108 68 41 48 99 323 380 378 282 365 332 447 2 1 0 2 37 9 6 7 41 10 16 18
Nucleus 72 129 88 84 73 144 165 207 161 158 138 164 145 155 125 88 130 121 93 184 131 76 85 93
Nuclear Periphery 1 0 2 2 2 4 1 2 9 10 4 15 0 0 0 0 0 0 2 1 6 0 0 2
Nucleolus 0 0 1 1 1 1 1 4 4 2 6 1 0 0 0 0 0 0 1 1 3 0 1 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 4 2 3 2 3 10 5 2 4 2 2 2 1 5 1 7 12 14 8 11 13
Vac/Vac Membrane 21 74 54 39 55 57 39 77 42 55 65 51 10 20 22 54 138 109 51 77 104 64 95 108
Unique Cell Count 163 258 265 221 253 471 509 653 452 525 495 576 209 238 247 208 415 327 189 352 414 221 293 339
Labelled Cell Count 229 314 294 261 313 635 686 845 596 707 660 767 218 251 260 231 488 388 189 352 414 221 293 339


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.2 4.8 4.4 4.3 3.4 3.4 3.6 3.4 3.4 3.3 3.1 5.6 5.6 5.3 5.9 5.5 5.5 5.8 6.0 6.1
Std Deviation (1e-4) 0.5 0.6 2.3 2.1 1.9 1.2 1.4 1.8 0.8 0.7 1.1 0.5 1.0 1.0 1.0 1.5 1.2 1.3 1.5 1.4 1.2
Intensity Change (Log2) -0.13 -0.18 -0.5 -0.5 -0.45 -0.52 -0.53 -0.57 -0.65 0.21 0.2 0.13 0.29 0.18 0.18 0.26 0.3 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 1.4 3.6 3.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rif2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available