Standard name
Description 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.05 0.06 0.05 0.06 0 0 0.05 0 0.08 0.07 0.08 0.09 0.27 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.06 0.1 0.16 0.23 0.18 0.2 0 0.07 0 0.12 0.16 0.11 0 0 0 0 0 0
Nucleus 0.97 0.97 0.9 0.86 0.82 0.77 0.8 0.77 0.72 0.71 0.68 0.67 0.87 0.82 0.8 0.77 0.73 0.79 0.95 0.97 0.87 0.8 0.68 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.11 0.12 0.14 0.21 0.29 0.29 0.33 0.44 0.46 0.41 0.51 0.21 0.24 0.27 0.14 0.07 0.08 0 0 0 0.09 0.07 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.2 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 2 0 2 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 8 1 6 6 3 7 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 0 10 6 9 13 16 22 13 6 10 7 8 11 18 18 35 18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 1 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Mitochondria 0 0 1 7 7 28 20 37 47 74 38 53 3 11 10 26 20 17 0 0 0 0 1 0
Nucleus 239 117 155 143 138 176 267 300 216 229 146 179 87 121 182 160 94 117 235 119 148 69 109 84
Nuclear Periphery 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 25 13 20 24 35 66 96 127 133 150 87 136 21 35 62 29 9 12 4 0 7 7 11 22
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 1 2 1 0 2 2 1 1 0 1 0 0 3 5 5 6 3 1 6 5 32 46
Unique Cell Count 246 120 172 166 169 228 335 388 301 323 214 266 100 148 227 209 129 149 249 124 171 87 160 163
Labelled Cell Count 267 130 187 183 190 283 414 489 418 467 284 383 121 181 280 242 167 172 249 124 171 87 160 163


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 40.8 49.1 39.1 37.3 36.0 32.6 31.5 34.6 32.8 29.9 32.0 30.7 53.4 56.0 55.8 76.0 86.7 96.2 37.5 47.6 52.2
Std Deviation (1e-4) 11.9 16.3 18.2 15.4 15.1 15.6 16.3 16.6 13.9 12.8 16.0 12.9 21.4 23.4 27.8 38.2 50.8 47.9 15.4 21.5 22.5
Intensity Change (Log2) – – – -0.07 -0.12 -0.27 -0.31 -0.18 -0.26 -0.39 -0.29 -0.35 0.45 0.52 0.51 0.96 1.15 1.3 -0.06 0.28 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -0.2 0 -0.5 -0.1 -0.7 -2.4 -0.5 -1.7 0.7 0.6 0.8 1.0 5.1 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.5 2.9 3.9 5.2 6.6 5.6 6.0 0 3.2 0 4.5 5.0 4.2
Nucleus -1.1 -2.2 -3.4 -3.0 -3.6 -4.7 -4.9 -5.2 -5.5 -0.8 -2.2 -2.7 -3.5 -3.9 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 2.3 4.2 4.3 5.2 7.3 7.8 6.3 8.4 2.1 2.8 3.8 0.7 -1.4 -1.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0