Standard name
Description Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0.06 0 0 0 0 0 0
Cytoplasm 0.92 – 0.96 0.95 0.97 0.95 0.92 0.91 0.88 0.86 0.78 0.78 1.0 0.99 0.98 0.91 0.92 0.93 0.79 0.7 0.79 0.87 0.82 0.84
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.07 0.06 0.06 0.11 0.2 0.23 0.32 0.39 0.41 0.38 0 0 0 0.05 0 0 0 0.05 0.08 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 – 0 2 0 1 2 0 0 0 0 0 0 0 0 5 2 2 13 1 5 3 7 6
Bud 6 – 2 5 2 5 12 8 2 11 13 10 2 0 5 2 0 1 3 1 1 3 1 3
Bud Neck 0 – 0 0 1 3 3 4 0 2 0 1 0 0 0 0 0 0 7 1 0 2 8 4
Bud Site 0 – 0 0 0 0 0 0 2 0 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 – 0 6 10 3 2 3 2 3 2 1 1 4 8 19 10 10 2 0 1 0 1 3
Cytoplasm 255 – 106 199 238 225 366 438 261 284 228 238 204 355 381 193 110 153 240 33 98 130 209 177
Endoplasmic Reticulum 8 – 1 0 0 0 0 0 0 0 1 0 0 2 5 10 4 2 7 0 1 2 2 2
Endosome 1 – 0 0 0 0 0 1 0 0 0 0 0 0 0 3 3 3 3 1 0 1 1 1
Golgi 1 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 2 1 0 0 0 2
Mitochondria 1 – 8 12 14 27 80 112 94 130 121 116 0 0 8 10 2 2 9 2 10 3 8 5
Nucleus 0 – 1 0 0 0 2 0 2 0 3 4 1 0 0 2 0 0 0 1 0 0 2 0
Nuclear Periphery 1 – 0 0 0 0 0 2 0 0 1 0 0 0 0 0 1 0 1 0 0 0 1 0
Nucleolus 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 – 0 1 0 0 2 3 3 5 8 7 0 0 1 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 0 – 0 0 1 0 1 0 0 1 2 3 0 0 0 2 3 5 5 2 1 0 3 1
Unique Cell Count 277 110 210 246 237 398 480 296 330 292 306 204 357 388 213 119 164 303 48 124 150 254 212
Labelled Cell Count 296 118 225 266 264 470 572 366 436 381 382 208 362 408 247 135 178 303 48 124 150 254 212


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 – 3.8 3.6 3.9 3.5 3.2 3.2 2.9 2.8 2.8 2.4 5.0 5.0 4.4 7.4 8.2 8.2 5.1 4.9 5.7
Std Deviation (1e-4) 0.8 – 1.4 1.1 1.7 1.2 1.5 1.3 1.5 1.3 1.3 0.9 1.0 1.1 1.1 1.3 1.8 1.3 1.4 1.2 2.1
Intensity Change (Log2) – – – -0.1 0.04 -0.15 -0.26 -0.27 -0.4 -0.46 -0.46 -0.69 0.37 0.39 0.21 0.94 1.1 1.09 0.42 0.34 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0
Cytoplasm -0.6 0.2 -0.6 -1.6 -1.8 -2.5 -2.9 -4.4 -4.4 2.7 2.5 1.2 -1.9 -1.3 -1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -1.0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0682 1.522 1.6401 1.7652 1.1119 1.2306 1.936 2.231 2.402 2.199 2.6943 1.9112 0.4959 0.5611 0.703 1.1738 0.6037 0.6947
Actin 0.0314 0.0055 0.0192 0.0047 0.008 0.0138 0.0276 0.002 0.0061 0.0074 0.0467 0.0035 0.0237 0.0002 0.0143 0.001 0.0091 0.0051
Bud 0.0007 0.001 0.0011 0.0009 0.0033 0.0033 0.003 0.0012 0.0013 0.0018 0.0182 0.0013 0.0005 0.0001 0.0006 0.0004 0.0129 0.0019
Bud Neck 0.0024 0.0003 0.0091 0.0025 0.0014 0.0082 0.0048 0.0002 0.0045 0.0006 0.0025 0.0142 0.0008 0.0001 0.0002 0.0024 0.0006 0.0026
Bud Periphery 0.0015 0.0031 0.0022 0.0009 0.039 0.009 0.0037 0.0015 0.0022 0.0043 0.0756 0.0098 0.0013 0.0001 0.001 0.0008 0.1086 0.0269
Bud Site 0.0188 0.0047 0.0052 0.0105 0.0011 0.0017 0.0193 0.0056 0.0108 0.0041 0.0081 0.0009 0.0036 0.0003 0.0029 0.0004 0.0016 0.0001
Cell Periphery 0.0016 0.0029 0.0022 0.0008 0.0148 0.001 0.0014 0.0011 0.0006 0.0005 0.001 0.0027 0.0019 0.0003 0.0007 0.0012 0.0085 0.0016
Cytoplasm 0.4168 0.5888 0.5525 0.5351 0.5789 0.6355 0.4145 0.6219 0.6398 0.6241 0.3945 0.7157 0.1706 0.3412 0.3255 0.4941 0.5044 0.3471
Cytoplasmic Foci 0.0152 0.0053 0.0223 0.0133 0.0139 0.0101 0.0388 0.0067 0.0179 0.0084 0.0436 0.0067 0.0166 0.0017 0.0162 0.0059 0.0112 0.0137
Eisosomes 0.0004 0.0002 0.0006 0.0001 0.0004 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0008 0.0001 0.0005 0.0003 0.0006 0.0002
Endoplasmic Reticulum 0.0075 0.0047 0.0031 0.0026 0.0066 0.0029 0.0059 0.0022 0.0034 0.0065 0.0028 0.0018 0.0052 0.0008 0.0036 0.0043 0.0027 0.0012
Endosome 0.0201 0.002 0.022 0.028 0.0176 0.0048 0.0374 0.0034 0.0141 0.0158 0.0437 0.009 0.0113 0.0002 0.0082 0.0045 0.0026 0.0015
Golgi 0.0066 0.0011 0.0075 0.0147 0.0098 0.003 0.0085 0.0016 0.0072 0.0091 0.0305 0.0021 0.0046 0.0001 0.0028 0.0003 0.0024 0.0008
Lipid Particles 0.0226 0.0007 0.0133 0.0054 0.0031 0.0006 0.0217 0.0005 0.0027 0.0075 0.0075 0.0003 0.0031 0.0001 0.0034 0.0004 0.0146 0.0006
Mitochondria 0.0057 0.0013 0.014 0.0072 0.1054 0.0024 0.004 0.0093 0.0161 0.0119 0.0067 0.0005 0.0036 0.0001 0.0007 0.0005 0.009 0.0005
None 0.4118 0.3717 0.3122 0.3137 0.1765 0.2995 0.3369 0.3334 0.2649 0.2798 0.2957 0.2293 0.7211 0.6537 0.6137 0.4736 0.2745 0.5942
Nuclear Periphery 0.0113 0.0028 0.0012 0.0031 0.0008 0.0006 0.0101 0.0003 0.0009 0.003 0.0026 0.0003 0.0091 0.0001 0.0005 0.0009 0.0002 0.0001
Nucleolus 0.0004 0.0002 0.0002 0.0173 0.0002 0.0001 0.0013 0.0001 0.0002 0.0007 0.0004 0 0.0003 0.0001 0.0001 0.0004 0.0001 0
Nucleus 0.0047 0.0007 0.0007 0.0183 0.0008 0.0007 0.0169 0.0006 0.0008 0.0011 0.0023 0.0005 0.0009 0.0002 0.0005 0.0031 0.0004 0.0002
Peroxisomes 0.0085 0.0003 0.0064 0.002 0.0027 0.0011 0.0255 0.0055 0.0025 0.0094 0.0071 0.0004 0.016 0 0.0028 0.0002 0.0337 0.0011
Punctate Nuclear 0.006 0.0006 0.0026 0.0084 0.0002 0.0005 0.0093 0.0002 0.0007 0.0013 0.0078 0.0003 0.0006 0.0001 0.0005 0.0013 0.0003 0.0001
Vacuole 0.0037 0.0016 0.0021 0.0083 0.0111 0.001 0.008 0.0024 0.0027 0.0019 0.0019 0.0006 0.0024 0.0005 0.001 0.0036 0.0016 0.0005
Vacuole Periphery 0.0025 0.0004 0.0005 0.002 0.0044 0.0002 0.0013 0.0001 0.0005 0.0008 0.0006 0.0001 0.002 0 0.0002 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.5472 13.9466 9.5728 12.3695 15.6766 21.8298 13.7489 9.8744 9.9982 19.7408
Translational Efficiency 1.2474 1.2445 0.895 0.8433 1.0745 1.4485 0.9372 0.8224 0.9842 0.9204

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔