Standard name | |
---|---|
Description | Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | – | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 2 | 13 | 1 | 5 | 3 | 7 | 6 |
Bud | 6 | – | 2 | 5 | 2 | 5 | 12 | 8 | 2 | 11 | 13 | 10 | 2 | 0 | 5 | 2 | 0 | 1 | 3 | 1 | 1 | 3 | 1 | 3 |
Bud Neck | 0 | – | 0 | 0 | 1 | 3 | 3 | 4 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 1 | 0 | 2 | 8 | 4 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | – | 0 | 6 | 10 | 3 | 2 | 3 | 2 | 3 | 2 | 1 | 1 | 4 | 8 | 19 | 10 | 10 | 2 | 0 | 1 | 0 | 1 | 3 |
Cytoplasm | 255 | – | 106 | 199 | 238 | 225 | 366 | 438 | 261 | 284 | 228 | 238 | 204 | 355 | 381 | 193 | 110 | 153 | 240 | 33 | 98 | 130 | 209 | 177 |
Endoplasmic Reticulum | 8 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 5 | 10 | 4 | 2 | 7 | 0 | 1 | 2 | 2 | 2 |
Endosome | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 3 | 1 | 0 | 1 | 1 | 1 |
Golgi | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | – | 8 | 12 | 14 | 27 | 80 | 112 | 94 | 130 | 121 | 116 | 0 | 0 | 8 | 10 | 2 | 2 | 9 | 2 | 10 | 3 | 8 | 5 |
Nucleus | 0 | – | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 3 | 4 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 1 | 0 | 0 | 2 | 3 | 3 | 5 | 8 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 3 | 0 | 0 | 0 | 2 | 3 | 5 | 5 | 2 | 1 | 0 | 3 | 1 |
Unique Cell Count | 277 | 110 | 210 | 246 | 237 | 398 | 480 | 296 | 330 | 292 | 306 | 204 | 357 | 388 | 213 | 119 | 164 | 303 | 48 | 124 | 150 | 254 | 212 | |
Labelled Cell Count | 296 | 118 | 225 | 266 | 264 | 470 | 572 | 366 | 436 | 381 | 382 | 208 | 362 | 408 | 247 | 135 | 178 | 303 | 48 | 124 | 150 | 254 | 212 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | – | 3.8 | 3.6 | 3.9 | 3.5 | 3.2 | 3.2 | 2.9 | 2.8 | 2.8 | 2.4 | 5.0 | 5.0 | 4.4 | 7.4 | 8.2 | 8.2 | 5.1 | 4.9 | 5.7 |
Std Deviation (1e-4) | 0.8 | – | 1.4 | 1.1 | 1.7 | 1.2 | 1.5 | 1.3 | 1.5 | 1.3 | 1.3 | 0.9 | 1.0 | 1.1 | 1.1 | 1.3 | 1.8 | 1.3 | 1.4 | 1.2 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.04 | -0.15 | -0.26 | -0.27 | -0.4 | -0.46 | -0.46 | -0.69 | 0.37 | 0.39 | 0.21 | 0.94 | 1.1 | 1.09 | 0.42 | 0.34 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 |
Cytoplasm | -0.6 | 0.2 | -0.6 | -1.6 | -1.8 | -2.5 | -2.9 | -4.4 | -4.4 | 2.7 | 2.5 | 1.2 | -1.9 | -1.3 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0682 | 1.522 | 1.6401 | 1.7652 | 1.1119 | 1.2306 | 1.936 | 2.231 | 2.402 | 2.199 | 2.6943 | 1.9112 | 0.4959 | 0.5611 | 0.703 | 1.1738 | 0.6037 | 0.6947 |
Actin | 0.0314 | 0.0055 | 0.0192 | 0.0047 | 0.008 | 0.0138 | 0.0276 | 0.002 | 0.0061 | 0.0074 | 0.0467 | 0.0035 | 0.0237 | 0.0002 | 0.0143 | 0.001 | 0.0091 | 0.0051 |
Bud | 0.0007 | 0.001 | 0.0011 | 0.0009 | 0.0033 | 0.0033 | 0.003 | 0.0012 | 0.0013 | 0.0018 | 0.0182 | 0.0013 | 0.0005 | 0.0001 | 0.0006 | 0.0004 | 0.0129 | 0.0019 |
Bud Neck | 0.0024 | 0.0003 | 0.0091 | 0.0025 | 0.0014 | 0.0082 | 0.0048 | 0.0002 | 0.0045 | 0.0006 | 0.0025 | 0.0142 | 0.0008 | 0.0001 | 0.0002 | 0.0024 | 0.0006 | 0.0026 |
Bud Periphery | 0.0015 | 0.0031 | 0.0022 | 0.0009 | 0.039 | 0.009 | 0.0037 | 0.0015 | 0.0022 | 0.0043 | 0.0756 | 0.0098 | 0.0013 | 0.0001 | 0.001 | 0.0008 | 0.1086 | 0.0269 |
Bud Site | 0.0188 | 0.0047 | 0.0052 | 0.0105 | 0.0011 | 0.0017 | 0.0193 | 0.0056 | 0.0108 | 0.0041 | 0.0081 | 0.0009 | 0.0036 | 0.0003 | 0.0029 | 0.0004 | 0.0016 | 0.0001 |
Cell Periphery | 0.0016 | 0.0029 | 0.0022 | 0.0008 | 0.0148 | 0.001 | 0.0014 | 0.0011 | 0.0006 | 0.0005 | 0.001 | 0.0027 | 0.0019 | 0.0003 | 0.0007 | 0.0012 | 0.0085 | 0.0016 |
Cytoplasm | 0.4168 | 0.5888 | 0.5525 | 0.5351 | 0.5789 | 0.6355 | 0.4145 | 0.6219 | 0.6398 | 0.6241 | 0.3945 | 0.7157 | 0.1706 | 0.3412 | 0.3255 | 0.4941 | 0.5044 | 0.3471 |
Cytoplasmic Foci | 0.0152 | 0.0053 | 0.0223 | 0.0133 | 0.0139 | 0.0101 | 0.0388 | 0.0067 | 0.0179 | 0.0084 | 0.0436 | 0.0067 | 0.0166 | 0.0017 | 0.0162 | 0.0059 | 0.0112 | 0.0137 |
Eisosomes | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0.0003 | 0.0006 | 0.0002 |
Endoplasmic Reticulum | 0.0075 | 0.0047 | 0.0031 | 0.0026 | 0.0066 | 0.0029 | 0.0059 | 0.0022 | 0.0034 | 0.0065 | 0.0028 | 0.0018 | 0.0052 | 0.0008 | 0.0036 | 0.0043 | 0.0027 | 0.0012 |
Endosome | 0.0201 | 0.002 | 0.022 | 0.028 | 0.0176 | 0.0048 | 0.0374 | 0.0034 | 0.0141 | 0.0158 | 0.0437 | 0.009 | 0.0113 | 0.0002 | 0.0082 | 0.0045 | 0.0026 | 0.0015 |
Golgi | 0.0066 | 0.0011 | 0.0075 | 0.0147 | 0.0098 | 0.003 | 0.0085 | 0.0016 | 0.0072 | 0.0091 | 0.0305 | 0.0021 | 0.0046 | 0.0001 | 0.0028 | 0.0003 | 0.0024 | 0.0008 |
Lipid Particles | 0.0226 | 0.0007 | 0.0133 | 0.0054 | 0.0031 | 0.0006 | 0.0217 | 0.0005 | 0.0027 | 0.0075 | 0.0075 | 0.0003 | 0.0031 | 0.0001 | 0.0034 | 0.0004 | 0.0146 | 0.0006 |
Mitochondria | 0.0057 | 0.0013 | 0.014 | 0.0072 | 0.1054 | 0.0024 | 0.004 | 0.0093 | 0.0161 | 0.0119 | 0.0067 | 0.0005 | 0.0036 | 0.0001 | 0.0007 | 0.0005 | 0.009 | 0.0005 |
None | 0.4118 | 0.3717 | 0.3122 | 0.3137 | 0.1765 | 0.2995 | 0.3369 | 0.3334 | 0.2649 | 0.2798 | 0.2957 | 0.2293 | 0.7211 | 0.6537 | 0.6137 | 0.4736 | 0.2745 | 0.5942 |
Nuclear Periphery | 0.0113 | 0.0028 | 0.0012 | 0.0031 | 0.0008 | 0.0006 | 0.0101 | 0.0003 | 0.0009 | 0.003 | 0.0026 | 0.0003 | 0.0091 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0.0001 |
Nucleolus | 0.0004 | 0.0002 | 0.0002 | 0.0173 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 |
Nucleus | 0.0047 | 0.0007 | 0.0007 | 0.0183 | 0.0008 | 0.0007 | 0.0169 | 0.0006 | 0.0008 | 0.0011 | 0.0023 | 0.0005 | 0.0009 | 0.0002 | 0.0005 | 0.0031 | 0.0004 | 0.0002 |
Peroxisomes | 0.0085 | 0.0003 | 0.0064 | 0.002 | 0.0027 | 0.0011 | 0.0255 | 0.0055 | 0.0025 | 0.0094 | 0.0071 | 0.0004 | 0.016 | 0 | 0.0028 | 0.0002 | 0.0337 | 0.0011 |
Punctate Nuclear | 0.006 | 0.0006 | 0.0026 | 0.0084 | 0.0002 | 0.0005 | 0.0093 | 0.0002 | 0.0007 | 0.0013 | 0.0078 | 0.0003 | 0.0006 | 0.0001 | 0.0005 | 0.0013 | 0.0003 | 0.0001 |
Vacuole | 0.0037 | 0.0016 | 0.0021 | 0.0083 | 0.0111 | 0.001 | 0.008 | 0.0024 | 0.0027 | 0.0019 | 0.0019 | 0.0006 | 0.0024 | 0.0005 | 0.001 | 0.0036 | 0.0016 | 0.0005 |
Vacuole Periphery | 0.0025 | 0.0004 | 0.0005 | 0.002 | 0.0044 | 0.0002 | 0.0013 | 0.0001 | 0.0005 | 0.0008 | 0.0006 | 0.0001 | 0.002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.5472 | 13.9466 | 9.5728 | 12.3695 | 15.6766 | 21.8298 | 13.7489 | 9.8744 | 9.9982 | 19.7408 |
Translational Efficiency | 1.2474 | 1.2445 | 0.895 | 0.8433 | 1.0745 | 1.4485 | 0.9372 | 0.8224 | 0.9842 | 0.9204 |
Dataset | Hit |
---|---|
Protein Abundance | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |