Standard name
Human Ortholog
Description Protein required for nuclear retention of unspliced pre-mRNAs; required along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0.13 0.18 0.21 0.11 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0.37 0.15 0.25 0 0 0.08 0 0 0 0 0.35 0.39 0.45 0.39 0.5 0.42 0.14 0.11 0.18 0.15 0.16 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.72 0.62 0.46 0.65 0.54 0.87 0.85 0.75 0.83 0.88 0.89 0.86 0.21 0.06 0.1 0.15 0.13 0.12 0 0.05 0.06 0.1 0.09 0.11
Nucleus 0.06 0 0 0 0.06 0.1 0.06 0.05 0.12 0.12 0.1 0.1 0.06 0.05 0.05 0.08 0 0.06 0 0 0 0.05 0 0
Nuclear Periphery 0.47 0.39 0.28 0.26 0.14 0.08 0.06 0.1 0.1 0.11 0.1 0.12 0.22 0.18 0.06 0.24 0.31 0.26 0.62 0.62 0.6 0.46 0.45 0.47
Nucleolus 0.08 0.2 0.06 0.05 0 0 0.05 0.07 0.06 0.06 0.07 0.05 0 0 0 0.07 0.07 0.1 0 0.05 0 0.05 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0 0 0.06 0.06 0.06 0 0 0.05 0.07 0.06 0.09 0.08 0.06 0.06 0 0.07 0.05 0.08 0.1 0.05
Vac/Vac Membrane 0.19 0.22 0.17 0.05 0.12 0 0.05 0 0 0 0 0.05 0.16 0.27 0.22 0.32 0.25 0.35 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 2 2 2 3
Bud 0 0 1 3 0 8 26 7 17 17 18 19 0 0 0 1 4 0 1 1 0 0 1 3
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 1 0 1 2 1 2
Bud Site 1 0 0 1 2 8 11 11 10 15 15 22 0 0 0 1 1 0
Cell Periphery 1 0 0 0 0 0 0 1 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 4 4 8 14 16 10 15 20 12 8 7 17 41 66 89 27 24 15 0 0 2 3 9 5
Endoplasmic Reticulum 2 1 0 0 2 0 1 0 0 0 0 0 2 1 0 12 10 9 0 0 0 0 1 1
Endosome 8 1 102 34 73 14 19 34 11 0 4 5 106 145 192 99 109 74 40 10 59 53 68 107
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4 2 1 4
Mitochondria 179 53 127 142 156 321 421 336 313 383 349 397 64 24 42 37 29 21 12 4 20 34 38 57
Nucleus 15 2 4 9 18 35 28 24 46 52 40 46 19 18 22 20 4 10 8 2 0 18 15 14
Nuclear Periphery 116 33 78 58 39 30 32 44 38 47 40 57 68 67 25 62 66 46 175 57 193 166 191 256
Nucleolus 19 17 17 11 7 14 26 30 23 28 27 24 11 1 3 19 16 17 11 4 4 18 17 16
Peroxisomes 0 1 17 7 12 4 9 19 5 3 2 4 6 19 35 7 0 3 3 0 5 4 5 8
SpindlePole 2 0 12 15 8 15 30 26 22 15 9 21 21 23 38 21 13 10 7 6 17 28 44 29
Vac/Vac Membrane 48 19 46 12 34 9 27 20 12 16 15 22 49 101 94 81 54 62 13 2 4 11 15 16
Unique Cell Count 249 85 277 220 287 368 493 451 377 433 390 464 307 371 428 255 216 175 284 93 325 359 429 542
Labelled Cell Count 395 131 412 306 367 468 646 572 510 585 526 635 388 465 542 387 332 267 284 93 325 359 429 542


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.0 5.2 4.9 4.8 3.7 3.8 4.1 3.9 3.5 3.3 3.4 6.1 6.1 5.9 6.4 6.4 6.3 6.0 6.1 6.8
Std Deviation (1e-4) 0.6 0.8 1.8 2.7 2.4 1.2 1.1 1.6 1.9 0.7 1.0 1.3 1.7 1.7 1.8 1.3 1.6 1.4 1.2 1.4 1.6
Intensity Change (Log2) -0.1 -0.11 -0.48 -0.46 -0.36 -0.42 -0.6 -0.65 -0.61 0.21 0.22 0.18 0.28 0.29 0.27 0.2 0.22 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 1.6 -0.1 0.1 1.1 0.2 -0.9 -0.9 0.6 4.6 5.9 6.7 3.6 3.7 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 -0.6 0.6 2.1 0.5 3.0 1.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.4 -11.7 -10.9 -7.8 -7.7 -7.5
Nucleus 1.8 3.0 4.3 2.8 2.6 5.1 5.1 4.5 4.4 2.9 2.4 2.5 3.6 0 2.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 -1.7 -3.1 -8.2 -1.0 0.6 -0.4
Nucleolus 0 0 0 0 0 0 0 0 0 -1.4 -4.5 -4.2 0.6 0.6 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 -2.6 -0.6 1.0 -1.9 -3.7 -2.2
SpindlePole 0 0 0 0 0 0 0 0 0 1.3 1.0 2.3 1.9 0.8 0.7
Vacuole -3.8 -1.6 -6.4 -5.1 -5.6 -6.0 -5.9 -5.6 -5.4 -0.2 3.2 1.7 4.1 2.3 4.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4553 0.5268 0.4469 0.6363 -0.0488 0.4538 0.8557 0.6528 0.538 0.1163 0.6672 0.3992 -0.284 -0.4164 -0.1807 -0.2523 0.1111 -0.3169
Actin 0.0067 0.0003 0.0008 0.0028 0.0455 0.0005 0.0135 0.0009 0.005 0.0163 0.01 0.0015 0.0244 0.0005 0.0066 0.0037 0.0066 0.0006
Bud 0.0001 0 0 0 0.0001 0 0.0002 0 0.0002 0.0002 0.0002 0 0.0005 0 0.0002 0.0001 0.0004 0
Bud Neck 0.0007 0.0001 0.0001 0.0011 0.0003 0.0002 0.0012 0.0002 0.0002 0.0004 0.0019 0.0008 0.0006 0.0001 0.001 0.0002 0.0022 0.0004
Bud Periphery 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0006 0.0004 0.0006 0.0002 0.0007 0.0001 0.0003 0.0002 0.0013 0.0001
Bud Site 0.0011 0 0 0 0.0002 0 0.0019 0.0003 0.0004 0.0004 0.0014 0.0001 0.0035 0.0001 0.0033 0.0002 0.0087 0
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0004 0
Cytoplasm 0.0122 0.0015 0.0017 0.0085 0.0021 0.0067 0.0066 0.0041 0.0026 0.0014 0.0089 0.0009 0.0011 0.0016 0.0041 0.0032 0.0042 0.0006
Cytoplasmic Foci 0.0031 0.0007 0.0013 0.0014 0.0054 0.0016 0.0193 0.0025 0.0048 0.0045 0.0107 0.0014 0.01 0.0022 0.0149 0.0038 0.0186 0.0021
Eisosomes 0.0002 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0004 0.0001 0.0003 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.002 0.0051 0.0021 0.0043 0.0008 0.0009 0.0045 0.0051 0.0046 0.0028 0.008 0.0017 0.0028 0.0037 0.0027 0.0047 0.0035 0.0007
Endosome 0.007 0.0047 0.0036 0.006 0.0077 0.0034 0.0328 0.0143 0.0102 0.012 0.0635 0.0062 0.03 0.011 0.0253 0.0099 0.0262 0.0063
Golgi 0.0027 0.0006 0.0008 0.0005 0.0047 0.0007 0.0067 0.002 0.0052 0.0057 0.0168 0.0034 0.018 0.0016 0.0153 0.003 0.015 0.0037
Lipid Particles 0.0338 0.0181 0.0179 0.0051 0.0803 0.0258 0.0467 0.0285 0.0262 0.0289 0.0541 0.0256 0.0829 0.0359 0.0441 0.063 0.0538 0.0319
Mitochondria 0.0024 0.0009 0.0015 0.0014 0.0059 0.0009 0.0024 0.001 0.0083 0.008 0.0082 0.0029 0.0181 0.0007 0.0092 0.0055 0.0172 0.0013
None 0.0207 0.0132 0.0193 0.0688 0.0108 0.019 0.0047 0.0017 0.0048 0.0027 0.0195 0.0013 0.0028 0.0025 0.0022 0.0033 0.0572 0.0005
Nuclear Periphery 0.8266 0.8705 0.859 0.7099 0.7499 0.8394 0.6849 0.7743 0.8031 0.7687 0.6844 0.7907 0.6995 0.8197 0.7698 0.7322 0.6692 0.8294
Nucleolus 0.0078 0.0118 0.0065 0.0233 0.0027 0.0098 0.0078 0.014 0.0086 0.0055 0.0091 0.0306 0.0102 0.0126 0.0125 0.0043 0.03 0.0124
Nucleus 0.0285 0.0324 0.0178 0.0484 0.019 0.0359 0.0283 0.0654 0.0474 0.0363 0.0511 0.06 0.0188 0.0595 0.0282 0.0821 0.0126 0.0437
Peroxisomes 0.003 0.0002 0.0005 0.0005 0.011 0.0006 0.0072 0.001 0.0062 0.0045 0.0055 0.0008 0.0089 0.0005 0.0068 0.0045 0.0061 0.0006
Punctate Nuclear 0.0307 0.0247 0.0523 0.1061 0.0438 0.0476 0.1186 0.0689 0.0487 0.0897 0.0295 0.0635 0.0472 0.0324 0.0405 0.0596 0.0518 0.059
Vacuole 0.0009 0.0012 0.0007 0.0012 0.0006 0.0006 0.0033 0.0038 0.0011 0.0007 0.0029 0.001 0.0059 0.0021 0.0015 0.0011 0.0036 0.0006
Vacuole Periphery 0.0097 0.0136 0.0141 0.0103 0.0086 0.0062 0.0089 0.0117 0.0113 0.0106 0.0131 0.0073 0.0138 0.0132 0.0113 0.0153 0.0109 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1364 7.8518 10.9244 10.5049 14.9467 6.5838 12.4045 7.0327 10.4886 8.4054
Translational Efficiency 1.0495 0.8889 0.7663 0.7095 0.4454 1.1868 0.9443 1.4538 0.9636 1.1793

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1280 410 1589 1384 1612 358 282 1419 2892 768 1871 2803

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.74 861.87 933.03 830.73 741.23 797.32 975.11 900.35 733.93 831.78 939.37 865.97
Standard Deviation 91.51 113.15 114.70 126.11 91.81 98.23 105.42 123.36 92.04 111.22 114.35 129.49
Intensity Change Log 2 0.250007 0.364460 0.196916 0.105238 0.395644 0.280565 0.178624 0.380311 0.239817

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000144 0.000155 0.000550 0.000523 0.000100 0.000796 0.000843 0.000299 0.000120 0.000454 0.000594 0.000410
Bud Neck 0.000722 0.000460 0.002425 0.002209 0.000369 0.000399 0.001073 0.001012 0.000525 0.000432 0.002221 0.001603
Bud Site 0.000136 0.000393 0.000478 0.002949 0.000078 0.000268 0.000291 0.001561 0.000103 0.000335 0.000450 0.002246
Cell Periphery 0.000043 0.000025 0.000032 0.000058 0.000038 0.000045 0.000052 0.000022 0.000040 0.000034 0.000035 0.000040
Cytoplasm 0.006983 0.002550 0.003831 0.009915 0.012961 0.005896 0.006574 0.005253 0.010315 0.004110 0.004244 0.007555
Cytoplasmic Foci 0.006099 0.003914 0.000419 0.002023 0.005149 0.007713 0.001803 0.003985 0.005569 0.005685 0.000627 0.003017
Eisosomes 0.000020 0.000009 0.000014 0.000016 0.000017 0.000027 0.000044 0.000008 0.000018 0.000017 0.000018 0.000012
Endoplasmic Reticulum 0.005257 0.004977 0.005738 0.008451 0.004310 0.004280 0.010548 0.004501 0.004729 0.004652 0.006463 0.006452
Endosome 0.004569 0.003781 0.001428 0.005499 0.003036 0.006703 0.002187 0.005372 0.003715 0.005143 0.001542 0.005435
Golgi 0.001435 0.000859 0.000148 0.000701 0.001047 0.003989 0.000179 0.002228 0.001219 0.002318 0.000153 0.001474
Lipid Particles 0.104844* 0.045989 0.001126 0.005803 0.057305 0.061227 0.009010 0.006854 0.078346 0.053092 0.002314 0.006335
Mitochondria 0.008159 0.005286 0.002653 0.003484 0.005835 0.010869 0.002328 0.001733 0.006864 0.007888 0.002604 0.002598
Mitotic Spindle 0.016361 0.051683 0.125371* 0.189009* 0.035954 0.042998 0.076311 0.272237* 0.027282 0.047635 0.117976* 0.231142*
None 0.004828 0.000478 0.001111 0.001650 0.006645 0.001739 0.002622 0.000536 0.005841 0.001066 0.001339 0.001086
Nuclear Periphery 0.072838 0.070867 0.182843* 0.134962* 0.071763 0.096496 0.137484* 0.125236* 0.072239 0.082814 0.176006* 0.130038*
Nuclear Periphery Foci 0.713125* 0.787265* 0.496975* 0.572786* 0.725796* 0.669700* 0.558774* 0.543158* 0.720188* 0.732462* 0.506290* 0.557787*
Nucleolus 0.027861 0.008377 0.073801 0.015016 0.038024 0.036539 0.059600 0.006563 0.033526 0.021505 0.071660 0.010737
Nucleus 0.023843 0.010496 0.098136 0.034810 0.030029 0.041813 0.124625 0.011094 0.027292 0.025094 0.102128 0.022804
Peroxisomes 0.001445 0.000706 0.000344 0.000896 0.000694 0.002176 0.001107 0.000576 0.001026 0.001391 0.000459 0.000734
Vacuole 0.000776 0.000830 0.002088 0.007342 0.000511 0.002990 0.004272 0.005978 0.000628 0.001836 0.002417 0.006651
Vacuole Periphery 0.000510 0.000900 0.000490 0.001897 0.000339 0.003338 0.000273 0.001794 0.000415 0.002037 0.000457 0.001845

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.04 -11.48 -3.60 -3.50 0.86 -1.30 -7.61 -3.75 0.74 5.50 -1.31 -14.63 -5.11 -0.14 3.83
Bud Neck 0.60 -4.88 -4.08 -6.08 1.46 -0.74 -6.11 -7.71 -6.83 1.48 0.40 -7.70 -6.84 -8.09 3.09
Bud Site -0.84 -4.67 -3.51 -3.05 -2.83 -1.65 -4.07 -2.72 -2.39 -1.14 -1.39 -6.22 -4.44 -3.85 -3.19
Cell Periphery 3.38 1.40 -0.99 -2.21 -1.43 -0.80 -5.12 3.98 2.44 6.81 1.36 -0.85 -0.12 -0.88 0.23
Cytoplasm 2.34 1.81 -2.40 -4.20 -3.97 2.96 2.13 2.87 -0.79 0.15 4.05 4.39 0.66 -3.62 -3.94
Cytoplasmic Foci 1.94 6.40 4.35 1.37 -4.37 -1.19 2.97 2.11 2.30 -0.95 0.38 8.99 4.65 2.58 -5.32
Eisosomes 5.44 2.31 1.42 -3.15 -0.24 -2.70 -7.06 4.71 4.71 7.91 0.94 -3.40 3.53 1.54 5.77
Endoplasmic Reticulum 0.25 -1.92 -4.33 -3.83 -3.19 -0.06 -7.93 -1.26 -1.32 7.43 0.04 -5.80 -4.36 -3.83 1.23
Endosome 0.69 3.21 -1.11 -2.12 -6.26 -1.80 -1.44 -3.56 0.45 -2.00 -1.28 3.76 -3.17 -0.58 -7.55
Golgi 1.88 5.49 2.78 0.42 -3.18 -1.76 4.37 -1.27 1.07 -2.57 -1.23 7.31 -0.15 1.05 -3.36
Lipid Particles 7.96 20.51 19.41 7.12 -6.99 -0.66 12.11 15.92 7.22 2.10 4.54 26.31 24.85 10.06 -5.84
Mitochondria 1.65 5.03 4.00 0.98 -1.40 -1.49 6.22 6.57 2.85 1.55 -0.40 7.17 6.77 2.98 0.08
Mitotic Spindle -4.52 -19.04 -21.55 -13.04 -6.13 -1.73 -3.98 -25.61* -19.40 -14.24 -4.09 -17.58 -32.84 -23.10 -14.40
None 5.46 4.50 3.68 -3.10 -1.04 4.60 2.98 7.54 2.60 2.24 7.41 7.73 8.00 0.41 0.93
Nuclear Periphery 0.19 -18.04 -10.94 -9.00 6.63 -3.25 -4.81 -9.39 -3.16 -0.11 -2.23 -20.17 -14.45 -8.43 7.72
Nuclear Periphery Foci -5.25 19.75 12.14 14.89 -6.84 3.41 10.25 16.96 6.90 -0.48 -0.96 24.89 20.63 14.89 -5.52
Nucleolus 7.05 -11.37 4.48 -3.76 16.94 0.23 -3.09 10.85 5.78 8.13 3.96 -10.79 11.10 3.67 20.28
Nucleus 4.06 -16.73 -3.88 -7.19 12.76 -2.09 -8.53 5.24 4.30 9.63 0.36 -18.67 0.25 -0.20 18.58
Peroxisomes 2.97 4.85 2.00 -0.95 -2.46 -2.13 -3.07 0.52 2.22 2.90 -0.63 4.45 1.82 1.57 -1.52
Vacuole -0.30 -4.07 -6.72 -6.60 -4.06 -1.85 -3.99 -7.16 -3.99 2.05 -1.90 -5.67 -9.83 -7.10 -2.62
Vacuole Periphery -1.20 -0.09 -3.56 -1.98 -3.53 -1.77 -0.43 -2.83 0.89 -2.79 -2.02 -1.10 -4.46 0.22 -4.14