Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) and ubiquitin receptor; involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a Ub-interacting CUE domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.89 0.89 0.84 0.77 0.73 0.62 0.65 0.6 0.6 0.98 0.99 0.99 0.97 0.95 0.96 0.91 0.96 0.92 0.95 0.94 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.1 0.3 0.36 0.36 0.51 0.48 0.51 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0.05 0.06 0.1 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 1 0
Bud 0 0 3 2 3 0 2 7 11 19 15 13 0 0 0 0 2 0 1 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 5 5 1 10 2 7 6 9 9 11 4 5 5 4 4 14 0 0 0 0 0 0
Cytoplasm 125 225 299 163 279 336 319 403 266 355 278 342 431 685 685 133 187 206 116 215 285 361 584 531
Endoplasmic Reticulum 1 1 2 3 1 2 1 2 3 3 0 3 4 2 3 9 8 3 0 1 3 1 3 0
Endosome 0 0 2 3 3 1 0 3 0 2 0 0 0 2 3 1 2 4 1 0 4 2 8 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 2 2
Mitochondria 5 2 5 15 31 120 151 197 218 259 235 312 2 0 0 0 3 1 0 1 4 1 3 1
Nucleus 2 0 0 0 0 1 5 3 1 7 2 6 2 2 2 0 2 0 0 1 0 1 2 1
Nuclear Periphery 0 0 0 0 0 0 0 1 1 1 4 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 1 1 0 2 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 2 0 0 2 1 1
Vac/Vac Membrane 0 0 7 10 10 8 22 33 42 33 31 41 1 8 8 2 5 5 1 1 4 3 5 1
Unique Cell Count 128 228 309 183 314 400 415 551 426 545 461 570 439 695 695 137 197 214 129 223 311 381 622 552
Labelled Cell Count 133 228 324 201 329 479 504 657 550 688 576 730 445 706 707 150 215 237 129 223 311 381 622 552


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 10.5 8.3 7.6 7.0 5.8 5.5 5.4 4.9 5.0 4.5 4.5 9.5 9.4 9.4 12.2 11.8 12.2 9.6 9.4 10.2
Std Deviation (1e-4) 1.3 1.4 1.2 1.3 1.7 1.4 1.6 1.3 0.9 1.2 1.2 0.9 1.2 1.1 1.1 2.3 2.2 2.4 1.3 1.4 1.4
Intensity Change (Log2) – – – -0.14 -0.25 -0.51 -0.6 -0.62 -0.77 -0.74 -0.9 -0.89 0.19 0.17 0.17 0.55 0.5 0.56 0.21 0.18 0.29

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.6 2.2 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.8 0 0 0 0 0 0 0 0 0 0 0 3.0
Cytoplasm -3.4 -3.8 -5.5 -7.5 -8.6 -10.9 -10.5 -11.4 -11.7 1.2 1.9 1.9 0.2 -1.0 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 4.4 9.8 11.3 11.3 14.4 13.9 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.9 0.7 -0.2 2.1 2.5 4.1 2.5 2.8 3.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.8644 7.5249 6.9591 6.3659 6.2142 6.7904 3.7492 5.409 4.8811 4.4559 4.3324 4.7785 3.4293 5.2512 4.4774 4.1491 3.9857 4.3167
Actin 0.0081 0.0002 0.0049 0.0036 0.0007 0.0013 0.0143 0.0003 0.0105 0.0393 0.0302 0.0029 0.0309 0.0002 0.0004 0.0006 0.022 0.0095
Bud 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.001 0.0001 0.0003 0.0005 0.0004 0.0001 0.0017 0.0001 0.0001 0.0004 0.0056 0.0001
Bud Neck 0.0069 0.0001 0.0003 0.0004 0.0005 0.001 0.0019 0.0001 0.0004 0.0006 0.0007 0.0007 0.0007 0.0001 0.0001 0.0002 0.0008 0.0005
Bud Periphery 0.0002 0 0.0001 0.0001 0 0 0.0011 0 0.0003 0.0005 0.0008 0.0001 0.0016 0 0 0.0004 0.0039 0.0001
Bud Site 0.0007 0.0006 0.0005 0.0002 0.0002 0.0001 0.0016 0.0003 0.0017 0.0008 0.0025 0.0001 0.0029 0.0001 0.0002 0.0002 0.0055 0.0002
Cell Periphery 0.0002 0 0.0001 0 0 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0003 0 0 0 0.0002 0
Cytoplasm 0.8688 0.972 0.9541 0.9241 0.9433 0.9646 0.7882 0.9809 0.9219 0.8213 0.6538 0.9555 0.7715 0.9839 0.9832 0.8544 0.8352 0.9479
Cytoplasmic Foci 0.0188 0.0066 0.0102 0.0122 0.0086 0.009 0.0389 0.0027 0.0159 0.0477 0.0455 0.0087 0.0503 0.006 0.0042 0.0504 0.0214 0.0082
Eisosomes 0.0002 0 0 0 0 0 0.0002 0 0 0.0001 0.0003 0 0.0003 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0052 0.0011 0.0022 0.0014 0.0017 0.0031 0.0098 0.0026 0.0021 0.0014 0.0028 0.0017 0.0134 0.001 0.0014 0.0026 0.0012 0.0026
Endosome 0.0109 0.0028 0.0053 0.0067 0.0045 0.0077 0.0458 0.0039 0.0155 0.0265 0.0735 0.0044 0.0416 0.001 0.0039 0.015 0.0269 0.0066
Golgi 0.0026 0.0002 0.0007 0.001 0.0002 0.0004 0.0101 0.0004 0.0019 0.0154 0.0338 0.0012 0.0138 0.0002 0.0002 0.0019 0.0502 0.0013
Lipid Particles 0.0027 0 0.0002 0.0005 0 0.0001 0.0042 0.0001 0.0049 0.0101 0.0152 0.0001 0.0118 0 0 0.0008 0.004 0.0007
Mitochondria 0.0036 0.0001 0.0002 0.0004 0.0001 0.0003 0.0156 0.0001 0.0012 0.0028 0.1089 0.0185 0.0087 0.0001 0.0001 0.0643 0.013 0.0007
None 0.0414 0.0132 0.0168 0.0256 0.0369 0.0061 0.0344 0.0065 0.0088 0.0036 0.0017 0.0021 0.0134 0.0056 0.0043 0.0023 0.0038 0.0046
Nuclear Periphery 0.0046 0.0004 0.0006 0.0005 0.0004 0.0023 0.0054 0.0006 0.0026 0.0034 0.002 0.0005 0.0162 0.0003 0.0004 0.0008 0.0007 0.0135
Nucleolus 0.0004 0 0 0 0 0 0.0008 0 0.0007 0.0001 0.0003 0 0.0002 0 0 0 0 0
Nucleus 0.0056 0.0014 0.0016 0.0018 0.0013 0.0021 0.0034 0.0009 0.0021 0.0016 0.0014 0.0017 0.0023 0.0006 0.0009 0.001 0.0016 0.0016
Peroxisomes 0.0105 0.0001 0.0005 0.0194 0.0001 0.0001 0.0102 0 0.0022 0.0192 0.0087 0.0009 0.0126 0.0005 0 0.002 0.0016 0.0005
Punctate Nuclear 0.0068 0.0003 0.0004 0.0003 0.0003 0.0004 0.0082 0.0001 0.0013 0.0037 0.0076 0.0001 0.0014 0.0001 0.0002 0.0004 0.0004 0.0004
Vacuole 0.0013 0.0005 0.0009 0.0013 0.0007 0.0008 0.0033 0.0003 0.0047 0.0009 0.007 0.0005 0.0025 0.0003 0.0003 0.0014 0.0011 0.0006
Vacuole Periphery 0.0004 0.0001 0.0002 0.0003 0.0002 0.0003 0.0015 0.0001 0.001 0.0004 0.0026 0.0002 0.002 0 0.0001 0.0008 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.0881 11.6387 9.7159 16.5219 24.7931 20.3317 17.6518 12.1619 16.4541 19.4244
Translational Efficiency 1.651 1.8363 1.5811 1.1697 0.9276 1.3257 1.0722 1.2857 1.2034 1.3356

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1129 533 1824 1071 2026 1974 139 1571 3155 2507 1963 2642

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 840.29 1122.25 1044.11 1109.79 923.09 1033.73 1173.52 1374.60 893.46 1052.55 1053.27 1267.25
Standard Deviation 131.15 168.36 130.17 155.93 114.29 132.77 139.17 198.24 126.96 145.67 134.98 223.90
Intensity Change Log 2 — 0.417435 0.313314 0.401327 — 0.163316 0.346299 0.574469 — 0.290000 0.330675 0.494548

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000417 0.000432 0.000281 0.001196 0.000143 0.000508 0.001214 0.001125 0.000241 0.000491 0.000347 0.001154
Bud Neck 0.002892 0.002026 0.001870 0.002864 0.002968 0.002344 0.002217 0.007422 0.002941 0.002277 0.001895 0.005574
Bud Site 0.014255 0.019545 0.009478 0.038479 0.007843 0.009870 0.024677 0.018360 0.010137 0.011927 0.010555 0.026515
Cell Periphery 0.000160 0.000117 0.000065 0.000105 0.000091 0.000044 0.000210 0.000028 0.000116 0.000059 0.000075 0.000059
Cytoplasm 0.580823 0.599270 0.746532 0.614293 0.664974 0.709698 0.520540 0.867466 0.634861 0.686220 0.730529 0.764836
Cytoplasmic Foci 0.275690 0.203250 0.036165 0.060113 0.172471 0.141073 0.081585 0.010153 0.209407 0.154292 0.039381 0.030405
Eisosomes 0.000066 0.000022 0.000014 0.000021 0.000031 0.000016 0.000063 0.000009 0.000044 0.000017 0.000017 0.000014
Endoplasmic Reticulum 0.005071 0.003775 0.001084 0.000724 0.003775 0.000761 0.002517 0.000179 0.004239 0.001402 0.001186 0.000400
Endosome 0.016570 0.024701 0.004235 0.008489 0.011301 0.009785 0.013055 0.001990 0.013187 0.012956 0.004860 0.004625
Golgi 0.009373 0.003488 0.000227 0.009026 0.004104 0.003571 0.001864 0.001383 0.005990 0.003553 0.000343 0.004481
Lipid Particles 0.004861 0.003220 0.001184 0.001318 0.002854 0.001191 0.003559 0.000208 0.003572 0.001622 0.001352 0.000658
Mitochondria 0.002580 0.000478 0.000267 0.005322 0.001937 0.001453 0.007402 0.004331 0.002167 0.001246 0.000772 0.004733
Mitotic Spindle 0.000827 0.008305 0.011892 0.047847 0.002257 0.008072 0.020920 0.015707 0.001745 0.008122 0.012531 0.028736
None 0.001973 0.001067 0.002614 0.001064 0.002138 0.001164 0.002445 0.002266 0.002079 0.001143 0.002602 0.001779
Nuclear Periphery 0.000147 0.000159 0.000659 0.000836 0.000189 0.000167 0.000549 0.000069 0.000174 0.000165 0.000651 0.000380
Nuclear Periphery Foci 0.002016 0.003530 0.003244 0.002555 0.001172 0.000897 0.014828 0.000041 0.001474 0.001457 0.004064 0.001060
Nucleolus 0.000932 0.001199 0.000240 0.000364 0.000369 0.000374 0.000162 0.000264 0.000570 0.000549 0.000235 0.000305
Nucleus 0.023578 0.013550 0.090899 0.030150 0.037211 0.021377 0.046970 0.031206 0.032332 0.019713 0.087788 0.030778
Peroxisomes 0.001919 0.001967 0.000998 0.002347 0.000987 0.002161 0.003236 0.002629 0.001320 0.002119 0.001156 0.002515
Vacuole 0.051660 0.104944 0.087762 0.167808 0.080677 0.084075 0.251059 0.033832 0.070294 0.088512 0.099325 0.088143
Vacuole Periphery 0.004193 0.004956 0.000289 0.005080 0.002508 0.001400 0.000928 0.001330 0.003111 0.002156 0.000335 0.002850

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.20 1.48 -1.67 -1.33 -3.04 -1.38 -2.34 -1.79 -0.50 0.73 -1.19 -2.57 -2.23 -0.65 -1.56
Bud Neck 2.40 1.57 2.29 -0.26 1.17 2.24 2.45 -1.90 -2.90 -3.09 2.95 2.65 -1.03 -2.70 -2.61
Bud Site -2.21 3.68 -1.73 0.48 -4.34 -1.88 -1.75 -0.81 0.80 1.57 -1.77 0.68 -2.13 -0.62 -2.28
Cell Periphery 2.98 8.53 6.58 3.09 -1.27 10.21 -4.18 15.15 9.31 7.47 10.49 7.17 12.80 3.66 7.11
Cytoplasm -1.22 -10.21 3.39 3.83 13.60 -5.01 7.74 -21.45 -15.37 -14.28 -6.73 -4.42 -10.34 -3.21 -5.08
Cytoplasmic Foci 7.50 36.22 34.20 18.87 -2.75 5.70 12.62 41.25 33.10 7.62 11.26 44.57 50.93 35.30 7.28
Eisosomes 3.91 4.45 4.21 1.57 -2.28 7.93 -3.50 13.32 5.33 6.31 6.27 5.74 7.54 3.96 6.43
Endoplasmic Reticulum 1.20 4.52 5.45 3.38 8.18 5.09 2.48 6.31 4.97 5.25 5.47 6.04 8.30 4.99 11.61
Endosome -2.73 10.24 10.04 8.76 0.55 1.98 2.17 17.54 14.94 5.19 0.60 14.13 18.39 15.41 5.39
Golgi 7.76 14.32 5.69 0.85 -2.02 0.98 5.61 10.55 4.54 0.40 4.46 18.78 8.85 3.23 -2.35
Lipid Particles 2.61 7.45 7.08 3.28 -1.44 8.13 -0.03 13.82 8.88 4.39 8.17 9.43 13.26 5.94 5.33
Mitochondria 2.39 2.64 1.01 -1.14 -1.39 0.90 -0.21 0.26 -0.51 0.29 1.83 3.86 0.90 -0.71 -2.36
Mitotic Spindle -2.99 -7.19 -6.77 -3.48 -3.00 -3.49 -1.38 -4.50 -0.79 -0.05 -4.65 -6.67 -8.04 -3.51 -1.28
None 1.49 -0.92 1.38 -0.55 6.83 3.61 -0.23 -0.89 -6.42 -0.13 3.46 -1.43 0.71 -4.73 3.65
Nuclear Periphery -0.34 -13.94 -2.96 -2.89 2.05 0.40 -8.13 -0.87 -0.94 8.35 0.18 -15.14 -3.05 -2.86 7.06
Nuclear Periphery Foci -1.72 -3.05 -0.90 1.51 3.33 1.32 -7.80 6.42 10.16 8.62 -0.60 -8.05 2.38 2.09 10.81
Nucleolus -0.21 2.07 2.11 0.94 0.37 -0.07 3.96 1.50 0.72 -3.18 0.13 2.54 2.41 1.18 -0.20
Nucleus 4.69 -25.13 -6.58 -12.56 19.59 11.27 -2.97 -4.99 -14.72 1.16 10.47 -25.22 -7.79 -18.04 20.47
Peroxisomes 0.07 3.90 3.76 2.27 -0.77 -4.23 -1.19 -1.03 2.43 0.94 -3.39 1.57 0.75 3.40 -0.89
Vacuole -8.12 -16.06 -30.11 -19.12 -19.40 -1.75 -10.99 -2.16 -0.40 10.59 -6.40 -15.42 -22.73 -16.82 -5.45
Vacuole Periphery -0.37 6.52 3.54 2.74 -2.08 3.98 6.52 5.11 2.23 -0.27 2.79 11.00 5.84 3.01 -2.04
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) and ubiquitin receptor; involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a Ub-interacting CUE domain
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Vps9

Vps9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available