Standard name
Description Guanine nucleotide exchange factor (GEF) and ubiquitin receptor; involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a Ub-interacting CUE domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.89 0.89 0.84 0.77 0.73 0.62 0.65 0.6 0.6 0.98 0.99 0.99 0.97 0.95 0.96 0.91 0.96 0.92 0.95 0.94 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.1 0.3 0.36 0.36 0.51 0.48 0.51 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0.05 0.06 0.1 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 1 0
Bud 0 0 3 2 3 0 2 7 11 19 15 13 0 0 0 0 2 0 1 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 5 5 1 10 2 7 6 9 9 11 4 5 5 4 4 14 0 0 0 0 0 0
Cytoplasm 125 225 299 163 279 336 319 403 266 355 278 342 431 685 685 133 187 206 116 215 285 361 584 531
Endoplasmic Reticulum 1 1 2 3 1 2 1 2 3 3 0 3 4 2 3 9 8 3 0 1 3 1 3 0
Endosome 0 0 2 3 3 1 0 3 0 2 0 0 0 2 3 1 2 4 1 0 4 2 8 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 2 2
Mitochondria 5 2 5 15 31 120 151 197 218 259 235 312 2 0 0 0 3 1 0 1 4 1 3 1
Nucleus 2 0 0 0 0 1 5 3 1 7 2 6 2 2 2 0 2 0 0 1 0 1 2 1
Nuclear Periphery 0 0 0 0 0 0 0 1 1 1 4 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 1 1 0 2 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 2 0 0 2 1 1
Vac/Vac Membrane 0 0 7 10 10 8 22 33 42 33 31 41 1 8 8 2 5 5 1 1 4 3 5 1
Unique Cell Count 128 228 309 183 314 400 415 551 426 545 461 570 439 695 695 137 197 214 129 223 311 381 622 552
Labelled Cell Count 133 228 324 201 329 479 504 657 550 688 576 730 445 706 707 150 215 237 129 223 311 381 622 552


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 10.5 8.3 7.6 7.0 5.8 5.5 5.4 4.9 5.0 4.5 4.5 9.5 9.4 9.4 12.2 11.8 12.2 9.6 9.4 10.2
Std Deviation (1e-4) 1.3 1.4 1.2 1.3 1.7 1.4 1.6 1.3 0.9 1.2 1.2 0.9 1.2 1.1 1.1 2.3 2.2 2.4 1.3 1.4 1.4
Intensity Change (Log2) -0.14 -0.25 -0.51 -0.6 -0.62 -0.77 -0.74 -0.9 -0.89 0.19 0.17 0.17 0.55 0.5 0.56 0.21 0.18 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.6 2.2 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.8 0 0 0 0 0 0 0 0 0 0 0 3.0
Cytoplasm -3.4 -3.8 -5.5 -7.5 -8.6 -10.9 -10.5 -11.4 -11.7 1.2 1.9 1.9 0.2 -1.0 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 4.4 9.8 11.3 11.3 14.4 13.9 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.9 0.7 -0.2 2.1 2.5 4.1 2.5 2.8 3.1 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available