Standard name
Description Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0.05 0 0.06 – 0.05 0 0.13 0.12 0.18 0.12 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0.3 0.5 0.29 0.66 0.58 0.56 0.79 – 0.79 0.77 0 0 0.1 0 0 0.05 0 0 0 0 0 0
Nucleus 0.93 0.95 0.92 0.87 0.88 0.81 0.76 0.78 0.68 – 0.69 0.66 0.85 0.84 0.75 0.86 0.84 0.9 0.91 0.91 0.88 0.87 0.75 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0.09 0.07 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud 0 0 0 1 1 1 3 3 7 – 12 11 0 0 0 0 0 0 2 0 0 0 2 5
Bud Neck 0 0 0 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 2 11 11 11 18 – 14 23 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 1 5 15 21 10 32 24 27 – 22 18 23 31 56 58 21 22 2 0 1 4 2 3
Endoplasmic Reticulum 0 0 0 1 0 0 1 0 0 – 0 0 1 1 2 17 6 9 0 0 0 0 0 1
Endosome 0 0 0 0 1 0 0 1 0 – 0 1 0 1 1 0 2 1 1 1 0 0 6 6
Golgi 0 1 0 0 0 0 0 1 0 – 0 0 0 1 0 0 0 1 2 1 0 0 1 1
Mitochondria 7 16 67 181 135 260 377 407 345 – 336 395 4 6 30 2 4 17 2 0 0 0 5 9
Nucleus 213 139 207 316 409 319 490 564 299 – 292 338 146 214 237 432 201 300 208 142 201 134 169 195
Nuclear Periphery 1 1 1 1 1 0 11 2 1 – 3 8 2 1 2 0 1 1 0 0 0 0 0 1
Nucleolus 1 4 1 4 5 3 17 27 10 – 11 14 0 5 11 1 0 1 3 0 1 2 6 30
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 1 5 8 4 – 3 2 0 1 3 5 1 5 0 1 1 0 1 5
Vac/Vac Membrane 2 3 2 2 1 4 8 6 4 – 2 8 3 5 4 16 18 6 4 4 19 10 21 59
Unique Cell Count 228 146 225 365 465 393 649 726 439 423 512 171 255 315 502 240 335 230 156 230 155 226 331
Labelled Cell Count 232 165 285 522 577 610 955 1054 715 696 818 179 267 350 531 254 363 230 156 230 155 226 331


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.6 4.2 4.2 4.4 3.7 3.5 3.6 3.2 – 3.2 3.1 6.7 6.1 5.2 9.5 8.8 8.5 6.3 5.9 6.7
Std Deviation (1e-4) 1.1 1.0 0.6 1.2 1.1 1.0 1.1 1.2 1.1 – 1.1 1.0 3.1 1.8 1.7 1.9 2.1 1.8 2.5 1.7 1.8
Intensity Change (Log2) – – – -0.0 0.06 -0.16 -0.24 -0.2 -0.39 – -0.38 -0.45 0.68 0.55 0.31 1.19 1.07 1.02 0.6 0.49 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.2 1.5 0.3 1.7 0.8 2.2 – 1.8 0.9 4.3 4.1 5.6 4.1 3.1 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.8 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -7.1 -8.3 -6.0 -12.5 -8.4 -8.0
Nucleus -2.0 -1.6 -3.6 -5.3 -4.8 -6.8 – -6.6 -7.4 -2.1 -2.7 -5.0 -2.3 -2.7 -1.0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 2.4 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 1.8 3.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3652 3.1172 2.5628 2.2604 2.1741 2.8363 3.3357 3.8841 3.4235 3.0396 2.763 3.4322 2.0665 1.8254 1.672 1.7807 1.2325 1.9345
Actin 0.0168 0 0.0222 0 0 0.0024 0.0055 0.0034 0.0045 0.0001 0.0011 0.0012 0.0167 0 0 0 0 0.0025
Bud 0.0007 0 0.0008 0 0 0.0001 0.0003 0 0.0005 0.0002 0.0002 0.0001 0.0002 0 0 0 0 0
Bud Neck 0.0028 0.0001 0.0005 0 0.0004 0.0011 0.0005 0.0001 0.0004 0.0004 0.0009 0.0007 0.0019 0.0002 0.0001 0 0.0001 0.0007
Bud Periphery 0.0014 0 0.0028 0 0.0001 0.0003 0.0003 0 0.0007 0.0002 0.0004 0.0002 0.0007 0 0.0001 0 0 0
Bud Site 0.0038 0 0.0088 0 0.0001 0.0002 0.0073 0.0001 0.0038 0.0021 0.0014 0.0002 0.0009 0 0.0002 0 0 0
Cell Periphery 0.0005 0 0.0019 0 0 0.0002 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0 0 0 0 0
Cytoplasm 0.0043 0.0004 0.0007 0 0.0001 0.0005 0.0031 0.0001 0.0032 0.0003 0.0007 0.0003 0.0022 0 0.0047 0.0011 0.0029 0.0002
Cytoplasmic Foci 0.0112 0.0001 0.0029 0 0 0.0013 0.0262 0.0007 0.008 0.0024 0.0098 0.0016 0.0058 0 0 0 0.0121 0.0009
Eisosomes 0.0002 0 0.0002 0 0 0.0005 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.003 0 0.0009 0 0 0.0006 0.0007 0.0001 0.0006 0.0001 0.0003 0.0002 0.0017 0 0 0 0.0002 0.0001
Endosome 0.0176 0.0003 0.0011 0 0 0.0022 0.011 0.0019 0.0078 0.0165 0.0547 0.0044 0.0077 0 0 0 0.0099 0.0005
Golgi 0.0059 0 0.0011 0 0 0.0005 0.0045 0.0014 0.002 0.0023 0.0238 0.0032 0.0023 0 0 0 0.0004 0.0006
Lipid Particles 0.0123 0.0001 0.0057 0 0 0.0015 0.0202 0.0016 0.0062 0.0024 0.0114 0.0004 0.0207 0 0 0 0.0077 0.0017
Mitochondria 0.0069 0.0001 0.0016 0 0.0003 0.0066 0.0185 0.0004 0.0013 0.0008 0.0046 0.0024 0.0027 0 0.0001 0.0001 0.0002 0.0001
None 0.0037 0.0008 0.0002 0 0.0001 0.0007 0.0019 0.0001 0.0007 0.0001 0.0002 0 0.0014 0 0.0058 0.005 0.0325 0.0002
Nuclear Periphery 0.0246 0.0019 0.0007 0.0009 0.0012 0.0025 0.0027 0.0009 0.0022 0.0012 0.0029 0.0002 0.0274 0.0007 0.0008 0.0036 0.0021 0.0007
Nucleolus 0.0161 0.0118 0.0083 0.0066 0.0387 0.031 0.0093 0.0093 0.0067 0.0092 0.0204 0.0168 0.0105 0.0118 0.011 0.0034 0.0129 0.0178
Nucleus 0.8343 0.9783 0.9325 0.9915 0.9574 0.9446 0.8504 0.9784 0.9345 0.9537 0.8533 0.9664 0.8768 0.9678 0.9765 0.9847 0.9122 0.9712
Peroxisomes 0.0139 0 0.0044 0 0 0.0004 0.0285 0.0004 0.0039 0.0007 0.0035 0.0011 0.0041 0 0 0 0.0009 0.001
Punctate Nuclear 0.0118 0.0059 0.0013 0.0009 0.0014 0.0021 0.0079 0.0006 0.0061 0.0004 0.0088 0.0002 0.0128 0.0194 0.0006 0.002 0.0052 0.0017
Vacuole 0.0061 0.0001 0.0012 0 0.0001 0.0004 0.0006 0.0001 0.0055 0.0059 0.001 0.0002 0.0015 0 0.0001 0 0.0003 0.0001
Vacuole Periphery 0.0022 0 0.0002 0 0.0001 0.0004 0.0003 0.0001 0.0011 0.0007 0.0007 0.0001 0.0016 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6435 3.4793 4.9054 7.4829 7.8217 6.2239 6.5081 8.5476 7.7462 8.2355
Translational Efficiency 0.6458 0.8561 0.5827 0.4819 0.4633 0.6142 0.4775 0.4435 0.5042 0.4283

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘