Standard name
Human Ortholog
Description HMG-CoA reductase; catalyzes conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to nuclear envelope; overproduction induces formation of karmellae; forms foci at nuclear periphery upon DNA replication stress; HMG1 has a paralog, HMG2, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg1 mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.09 0 0.05 0 0 0 0 0 0.05 0.12 0.14 0.19 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0.08 0.1 0 0.06 0.17 0.11 0.09 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.07 0.08 0.09 0.07 0.21 0.21 0.09 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Nucleus 0.05 0.08 0 0 0 0.12 0.11 0.14 0.28 0.3 0.24 0 0.08 0.08 0.11 0.16 0 0 0 0 0 0 0
Nuclear Periphery 0.7 0.52 0.8 0.73 0.62 0.61 0.63 0.56 0.42 0.44 0.49 0.59 0.56 0.44 0.47 0.38 0.3 0.89 0.68 0.73 0.67 0.63 0.54
Nucleolus 0 0 0.05 0 0.11 0.15 0.2 0.21 0.2 0.21 0.24 0.1 0 0.12 0.05 0.05 0.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.43 0.46 0.27 0.3 0.31 0.23 0.15 0.16 0.11 0.07 0.14 0.33 0.33 0.34 0.4 0.38 0.55 0 0.13 0 0.1 0.16 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 0 0 0 1 0 0 1 0 7 6 2 0 0 0 1 1 4 1 2 1
Bud 0 0 0 1 1 1 3 3 3 1 3 0 0 1 1 0 0 3 4 1 1 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 0 3 5 3 0 0 0 0 0 0
Cell Periphery 2 1 0 0 0 0 0 0 0 1 0 4 5 6 0 0 0 0 0 0 0 0 0
Cytoplasm 10 6 9 4 21 11 18 13 4 6 4 6 5 17 8 7 0 0 1 0 0 1 0
Endoplasmic Reticulum 10 3 5 4 2 1 2 1 0 1 0 12 2 3 1 3 2 16 18 54 16 29 15
Endosome 1 0 1 1 4 1 2 6 0 0 5 8 1 1 2 1 1 2 2 3 1 5 11
Golgi 0 0 0 0 1 0 1 0 0 0 0 0 0 2 0 0 0 0 2 3 2 2 1
Mitochondria 0 8 12 9 18 24 31 35 84 72 33 0 0 1 4 2 1 4 5 3 3 11 11
Nucleus 24 20 6 2 5 35 40 70 112 104 86 0 8 12 6 6 0 1 10 1 2 3 2
Nuclear Periphery 316 123 238 125 152 186 231 284 170 151 177 90 53 63 27 14 6 426 191 236 96 198 152
Nucleolus 10 6 15 7 26 44 74 107 79 73 89 15 2 17 3 2 2 1 0 1 1 3 6
Peroxisomes 0 0 0 0 0 1 1 2 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 2 1 4 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 195 109 80 52 76 69 54 80 45 23 52 50 31 48 23 14 11 20 37 11 14 52 67
Unique Cell Count 451 237 298 171 245 303 364 506 400 346 364 152 95 142 57 37 20 481 283 325 143 318 281
Labelled Cell Count 569 276 367 205 306 373 459 602 500 438 452 195 115 178 75 49 23 481 283 325 143 318 281


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.4 13.0 13.7 14.6 13.6 12.1 11.6 11.4 9.1 9.7 10.8 14.4 14.8 15.2 8.8 8.1 12.0 14.9 16.6 16.3
Std Deviation (1e-4) 4.5 3.5 3.3 3.6 3.4 2.7 3.3 3.0 3.3 3.6 3.1 3.1 3.2 3.9 2.8 2.4 3.5 4.0 4.5 3.9
Intensity Change (Log2) 0.1 -0.0 -0.18 -0.24 -0.26 -0.59 -0.49 -0.34 0.08 0.12 0.16 -0.64 -0.75 -0.18 0.13 0.29 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.8 0.4 1.2 -0.4 -2.0 -1.1 -1.8 0.5 0 3.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 3.3 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 1.7 2.0 2.3 1.7 6.4 6.3 2.6 0 0 0 0 0 0
Nucleus 0 0 4.6 4.5 5.5 9.1 9.4 8.0 0 0 3.2 0 0 0
Nuclear Periphery -1.7 -4.6 -5.0 -4.6 -6.8 -9.9 -9.4 -8.3 -4.7 -4.7 -7.5 -5.2 -5.6 -5.1
Nucleolus -0.5 2.4 3.9 5.7 6.2 5.6 5.9 6.8 1.9 0 2.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.8 1.1 -1.2 -3.8 -3.8 -5.3 -7.0 -4.0 1.3 1.1 1.5 2.1 1.4 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.6578 12.1428 13.0989 10.8182 11.8708 12.2794 14.4153 17.5498 15.7674 16.8519 14.3247 15.1691 13.4747 17.6308 15.298 15.7169 14.2493 14.8318
Actin 0.0144 0.0002 0.0168 0.0059 0.0062 0.0006 0.0235 0.0005 0.0005 0.0038 0.0087 0.0051 0.0027 0.0002 0.0205 0.0006 0.0011 0.0002
Bud 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0 0 0.0002 0.0001 0.0001 0.0002 0 0.0008 0 0.0006 0
Bud Neck 0.0024 0.0002 0.0042 0.0004 0.001 0.0001 0.0004 0.0001 0.0001 0.0004 0.0002 0.0005 0.0003 0 0.0002 0.0001 0.0003 0.0001
Bud Periphery 0.0002 0.0001 0.0005 0.0004 0.0003 0.0001 0.0002 0.0002 0.0002 0.0007 0.0001 0.0002 0.0007 0.0002 0.0011 0.0004 0.0019 0
Bud Site 0.0008 0.0001 0.001 0.0003 0.0001 0 0.0007 0 0 0.0018 0.0002 0.0001 0.0003 0 0.0036 0 0.0012 0
Cell Periphery 0.0002 0.0003 0.0006 0.0005 0.0002 0.0002 0.0002 0.0005 0.0006 0.0008 0.0001 0.0001 0.0009 0.0017 0.0008 0.0003 0.0007 0
Cytoplasm 0.0037 0.01 0.0017 0.0027 0.002 0.0005 0.003 0.0005 0.0007 0.0003 0.0005 0.0001 0.0224 0.0003 0.0109 0.0002 0.0009 0.0003
Cytoplasmic Foci 0.0014 0.0002 0.0008 0.0028 0.0005 0.0003 0.0118 0 0.0045 0.0011 0.0078 0.0012 0.009 0 0.0039 0 0.0014 0.0001
Eisosomes 0.0002 0 0.0006 0.0001 0.0001 0 0.0003 0 0 0.0001 0 0 0.0007 0 0.0001 0 0.0001 0.0002
Endoplasmic Reticulum 0.0087 0.0396 0.0517 0.0858 0.0486 0.0092 0.042 0.2841 0.2353 0.254 0.1143 0.0677 0.056 0.403 0.3075 0.2678 0.5308 0.0402
Endosome 0.0063 0.0025 0.0047 0.0077 0.0125 0.0016 0.0146 0.001 0.0029 0.0032 0.0056 0.0017 0.0488 0.001 0.0183 0.001 0.0353 0.0002
Golgi 0.0017 0 0.002 0.0043 0.002 0.0002 0.0044 0.0001 0.0008 0.0031 0.0055 0.0038 0.0023 0.0002 0.0195 0.0002 0.0048 0.0001
Lipid Particles 0.0017 0.0002 0.0017 0.0194 0.0012 0.0002 0.0162 0.0004 0.0125 0.0064 0.0088 0.0003 0.0356 0.0005 0.0018 0.0003 0.0062 0.0002
Mitochondria 0.0026 0.0003 0.0022 0.0055 0.003 0.0004 0.0013 0.0008 0.0011 0.0398 0.0017 0.002 0.0022 0.0008 0.0078 0.001 0.0237 0.0003
None 0.001 0.0001 0.0009 0.0007 0.0006 0.0001 0.0013 0.0001 0.0005 0.0001 0.0001 0 0.1248 0.0001 0.0008 0.0001 0.0014 0.0233
Nuclear Periphery 0.8308 0.7389 0.7824 0.7686 0.8089 0.9205 0.7777 0.6399 0.6633 0.6142 0.7702 0.8815 0.6146 0.5225 0.5325 0.6717 0.3355 0.8896
Nucleolus 0.0008 0.0009 0.001 0.0007 0.0015 0.0003 0.0005 0 0.0001 0.0002 0.0001 0 0.0012 0 0.0001 0 0.0009 0.0019
Nucleus 0.0909 0.1638 0.082 0.0271 0.0691 0.0373 0.0394 0.0077 0.0061 0.0122 0.0146 0.0027 0.0124 0.0101 0.0067 0.004 0.0037 0.0041
Peroxisomes 0.0003 0 0.0003 0.004 0.0001 0.0001 0.0011 0 0.0001 0.0052 0.0051 0.001 0.0007 0 0.001 0 0.0007 0
Punctate Nuclear 0.0032 0.0004 0.0013 0.0015 0.0016 0.0012 0.0137 0 0.0005 0.0001 0.0002 0.0001 0.0177 0.0001 0.0014 0.0001 0.0004 0.0032
Vacuole 0.0017 0.0174 0.0057 0.0257 0.0075 0.0066 0.0024 0.0088 0.0082 0.0062 0.0041 0.0018 0.0062 0.0082 0.0071 0.0041 0.0149 0.0006
Vacuole Periphery 0.0272 0.0248 0.0376 0.0356 0.0331 0.0205 0.0451 0.0553 0.062 0.0461 0.0523 0.03 0.0402 0.0511 0.0536 0.048 0.0338 0.0353

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.9178 38.3331 43.4766 51.5701 37.7428 33.7981 43.4064 50.1921 47.9035 48.0421
Translational Efficiency 0.6508 0.6644 0.7976 0.6477 0.5517 0.7021 0.6129 0.4528 0.5763 0.5187

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
497 1379 1149 1017 890 2132 2599 2110 1387 3511 3748 3127

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1138.96 1486.13 1593.68 1485.87 1071.75 1364.86 1589.40 1554.90 1095.83 1412.49 1590.71 1532.45
Standard Deviation 199.99 307.56 288.65 271.91 232.52 264.43 252.45 274.63 223.75 288.31 264.08 275.65
Intensity Change Log 2 0.383843 0.484645 0.383591 0.348785 0.568514 0.536853 0.366953 0.525914 0.459927

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002505 0.000767 0.000664 0.000583 0.002119 0.000695 0.000445 0.000319 0.002257 0.000723 0.000512 0.000405
Bud Neck 0.000877 0.000852 0.002934 0.006815 0.001843 0.000375 0.000755 0.001168 0.001497 0.000562 0.001423 0.003005
Bud Site 0.001823 0.000344 0.000275 0.002950 0.001869 0.000455 0.000177 0.000189 0.001853 0.000411 0.000207 0.001087
Cell Periphery 0.000180 0.000293 0.000081 0.000232 0.000635 0.000233 0.000073 0.000093 0.000472 0.000257 0.000075 0.000138
Cytoplasm 0.003004 0.000321 0.000282 0.003290 0.003360 0.000786 0.000514 0.000449 0.003232 0.000604 0.000443 0.001373
Cytoplasmic Foci 0.003677 0.000602 0.000696 0.003075 0.001608 0.000554 0.000361 0.000334 0.002349 0.000573 0.000464 0.001226
Eisosomes 0.000010 0.000026 0.000015 0.000012 0.000027 0.000007 0.000005 0.000004 0.000021 0.000015 0.000008 0.000007
Endoplasmic Reticulum 0.123359* 0.062181 0.004403 0.014295 0.130897* 0.067041 0.021995 0.021250 0.128196* 0.065132 0.016602 0.018988
Endosome 0.018437 0.005367 0.001707 0.009006 0.009762 0.005987 0.003077 0.003213 0.012871 0.005744 0.002657 0.005097
Golgi 0.004494 0.000462 0.000245 0.003938 0.001276 0.000593 0.000314 0.000348 0.002429 0.000542 0.000293 0.001515
Lipid Particles 0.011042 0.012062 0.001770 0.006521 0.011597 0.006698 0.001305 0.003445 0.011398 0.008805 0.001448 0.004446
Mitochondria 0.004102 0.009607 0.002986 0.004891 0.016152 0.006175 0.002188 0.001711 0.011834 0.007523 0.002432 0.002745
Mitotic Spindle 0.007661 0.000118 0.002459 0.028271 0.000802 0.000436 0.001028 0.001233 0.003260 0.000311 0.001467 0.010027
None 0.002495 0.001200 0.000397 0.000787 0.008069 0.001710 0.000394 0.000520 0.006072 0.001509 0.000395 0.000606
Nuclear Periphery 0.366049* 0.701239* 0.667279* 0.466071* 0.570975* 0.724440* 0.812986* 0.713787* 0.497545* 0.715327* 0.768317* 0.633222*
Nuclear Periphery Foci 0.427869* 0.127787 0.228165* 0.372474* 0.174898 0.161974 0.138221 0.231517* 0.265544* 0.148547 0.165795 0.277361*
Nucleolus 0.002789 0.003206 0.046408 0.024294 0.010742 0.002593 0.001908 0.004220 0.007892 0.002834 0.015550 0.010749
Nucleus 0.004667 0.002002 0.030880 0.020238 0.024782 0.005248 0.005843 0.003356 0.017574 0.003973 0.013518 0.008847
Peroxisomes 0.000408 0.000374 0.000693 0.000499 0.002011 0.000244 0.000070 0.000076 0.001437 0.000295 0.000261 0.000213
Vacuole 0.010468 0.033926 0.003005 0.018950 0.015213 0.007114 0.004312 0.005283 0.013513 0.017645 0.003912 0.009728
Vacuole Periphery 0.004082 0.037266 0.004656 0.012808 0.011365 0.006640 0.004031 0.007485 0.008755 0.018669 0.004222 0.009217

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.90 0.94 0.96 1.03 0.39 5.36 5.01 6.79 2.60 0.85 2.18 2.35 2.59 2.94 0.87
Bud Neck 0.42 -4.84 -7.37 -7.74 -5.09 2.66 1.85 0.93 -3.59 -1.77 2.61 0.10 -3.79 -8.27 -5.36
Bud Site 1.69 1.72 -0.90 -3.55 -3.59 2.45 2.98 3.02 1.19 0.30 2.94 3.30 1.26 -2.71 -3.45
Cell Periphery -2.54 2.73 -1.55 0.79 -4.71 4.08 5.86 5.63 4.49 -1.40 3.34 6.27 5.08 4.41 -4.56
Cytoplasm 2.07 2.08 0.07 -3.59 -3.61 2.86 3.11 3.25 1.60 0.93 3.49 3.62 2.26 -2.81 -3.20
Cytoplasmic Foci 2.34 2.28 0.23 -4.92 -4.93 2.62 3.02 3.05 0.82 -0.22 3.36 3.55 1.85 -3.63 -4.09
Eisosomes -2.99 -0.78 -0.51 3.34 0.45 4.35 3.97 5.01 2.95 0.42 2.16 3.52 4.49 4.72 0.45
Endoplasmic Reticulum 5.75 12.08 10.85 11.31 -4.94 8.27 15.19 15.23 12.60 0.43 10.10 19.20 18.64 16.64 -1.37
Endosome 6.17 8.08 4.03 -3.40 -7.19 3.33 6.53 6.48 3.95 -0.33 6.77 10.21 7.38 0.90 -5.40
Golgi 2.29 2.41 0.26 -3.43 -3.66 1.43 2.15 2.18 0.96 -0.25 2.70 3.10 1.23 -2.65 -3.53
Lipid Particles -0.42 4.19 1.71 2.89 -3.25 3.44 7.89 5.85 3.84 -4.05 2.06 8.68 5.48 5.03 -5.06
Mitochondria -2.77 0.62 -0.55 3.03 -1.98 5.59 8.27 8.56 6.27 1.32 3.16 7.50 7.05 6.70 -0.85
Mitotic Spindle 2.62 1.93 -4.18 -8.91 -8.11 1.30 -0.78 -1.23 -2.08 -0.42 2.78 1.75 -3.92 -8.97 -7.61
None 1.66 2.61 2.13 1.12 -2.44 3.32 4.05 3.98 4.01 -1.04 3.65 4.57 4.39 4.03 -2.24
Nuclear Periphery -22.51* -19.23 -5.77 21.37* 16.92 -13.16 -21.69 -12.29 1.57 15.55 -22.78 -28.58 -13.36 13.08 21.71
Nuclear Periphery Foci 21.53* 13.61 3.65 -27.70* -14.41 2.03 6.14 -8.42 -12.67 -17.98* 16.30 13.72 -1.48 -26.80 -22.55
Nucleolus 0.38 -10.85 -6.29 -6.90 4.18 4.83 5.26 3.51 -1.79 -2.54 4.50 -4.33 -1.78 -6.73 2.49
Nucleus 1.81 -7.62 -5.32 -7.96 2.64 5.43 5.19 5.99 1.59 2.26 5.75 1.53 3.35 -5.16 3.11
Peroxisomes 0.62 -1.32 -0.86 -2.18 0.40 2.76 3.01 3.00 6.62 -0.60 2.81 2.86 2.85 0.52 -0.01
Vacuole -6.35 3.30 -2.53 4.14 -7.84 4.01 5.39 4.94 2.14 -1.26 -1.85 6.23 2.08 5.73 -7.18
Vacuole Periphery -12.58 -0.39 -4.37 9.26 -5.43 4.35 7.02 3.14 -0.93 -4.02 -7.92 5.25 -0.44 7.98 -6.57