Standard name | |
---|---|
Human Ortholog | |
Description | Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 8 | 11 | 20 | 23 | 43 | 23 | 31 | 28 | 41 | 33 | 24 | 8 | 7 | 20 | 1 | 2 | 2 | 3 | 8 | 2 | 2 | 1 | 1 |
Bud | 0 | 0 | 0 | 2 | 3 | 6 | 8 | 16 | 13 | 10 | 2 | 13 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 10 | 10 | 6 | 10 | 10 | 7 | 15 | 13 | 15 | 9 | 25 | 11 | 25 | 54 | 5 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 52 | 26 | 50 | 63 | 64 | 62 | 58 | 55 | 83 | 53 | 69 | 21 | 34 | 34 | 14 | 8 | 8 | 1 | 6 | 1 | 2 | 3 | 3 |
Endoplasmic Reticulum | 30 | 463 | 289 | 210 | 218 | 204 | 241 | 235 | 156 | 297 | 148 | 158 | 244 | 165 | 205 | 2 | 0 | 0 | 73 | 630 | 352 | 191 | 173 | 228 |
Endosome | 0 | 0 | 3 | 2 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 2 |
Golgi | 0 | 13 | 4 | 21 | 5 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 8 | 3 | 6 | 7 | 3 | 4 | 0 | 3 | 1 | 1 | 2 | 4 |
Mitochondria | 0 | 0 | 0 | 1 | 2 | 6 | 15 | 23 | 14 | 25 | 22 | 35 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 4 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 6 | 32 | 17 | 15 | 22 | 37 | 37 | 15 | 53 | 27 | 27 | 13 | 3 | 9 | 0 | 0 | 0 | 0 | 10 | 10 | 4 | 4 | 4 |
Nucleolus | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 4 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 120 | 36 | 36 | 73 | 57 | 54 | 50 | 41 | 110 | 17 | 40 | 21 | 15 | 28 | 9 | 3 | 5 | 2 | 17 | 12 | 9 | 13 | 31 |
Unique Cell Count | 51 | 601 | 343 | 296 | 344 | 356 | 382 | 389 | 287 | 532 | 260 | 321 | 282 | 214 | 286 | 40 | 17 | 22 | 91 | 688 | 388 | 215 | 205 | 282 |
Labelled Cell Count | 57 | 675 | 412 | 365 | 415 | 418 | 451 | 472 | 346 | 640 | 315 | 398 | 326 | 254 | 358 | 43 | 18 | 23 | 91 | 688 | 388 | 215 | 205 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 34.6 | 26.6 | 16.6 | 15.3 | 14.0 | 13.1 | 11.6 | 11.5 | 11.8 | 11.0 | 11.2 | 11.1 | 20.1 | 18.4 | 18.3 | 25.7 | 27.8 | 26.0 | 28.6 | 24.8 | 23.9 |
Std Deviation (1e-4) | 19.5 | 6.9 | 4.4 | 3.7 | 3.7 | 3.1 | 2.8 | 2.6 | 2.8 | 2.8 | 2.7 | 2.6 | 4.8 | 4.9 | 4.8 | 7.1 | 7.5 | 7.7 | 7.3 | 6.8 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.24 | -0.34 | -0.52 | -0.53 | -0.5 | -0.59 | -0.57 | -0.58 | 0.28 | 0.15 | 0.14 | 0.63 | 0.74 | 0.65 | 0.78 | 0.58 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.1 | 2.1 | 4.4 | 1.8 | 2.8 | 3.4 | 2.7 | 4.4 | 2.5 | -0.3 | 0 | 2.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3.8 | 4.0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | 0 | -0.1 | -1.0 | 0.7 | 1.1 | -0.1 | 0.4 | 2.8 | 0.7 | 4.1 | 6.6 | 0 | 0 | 0 |
Cytoplasm | 3.6 | 4.2 | 4.1 | 3.6 | 3.1 | 4.3 | 3.5 | 4.6 | 5.1 | -0.1 | 3.1 | 1.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | -4.1 | -6.2 | -7.8 | -6.4 | -7.1 | -8.2 | -8.7 | -7.4 | -9.6 | 0.8 | -2.1 | -3.8 | -11.1 | -8.5 | -9.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.7 | 4.6 | 4.1 | 4.1 | 5.5 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.7 | -2.6 | -1.6 | 0.2 | 0.1 | -2.0 | 0.3 | 0.4 | -0.4 | -2.3 | -3.8 | -3.1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.7 | 3.8 | 2.1 | 1.5 | 1.0 | 1.4 | 3.9 | -1.7 | 0.8 | -1.3 | -1.4 | -0.3 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.395 | 25.3188 | 23.3264 | 23.5232 | 21.3407 | 23.8351 | 25.2477 | 32.367 | 26.7378 | 23.0871 | 25.2464 | 31.0968 | 23.4826 | 25.1357 | 24.756 | 23.2964 | 24.3227 | 25.9997 |
Actin | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0183 | 0.0002 | 0.0166 | 0.0194 | 0.0132 | 0.0004 | 0.0059 | 0.0001 | 0.0013 | 0.0169 | 0.0165 | 0.001 |
Bud | 0 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0005 | 0.0038 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0012 | 0.0004 | 0 |
Bud Neck | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0011 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0024 | 0.0006 | 0.0004 |
Bud Periphery | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0036 | 0.0004 | 0.0014 | 0.0202 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0018 | 0.0015 | 0.0001 |
Bud Site | 0.0003 | 0.002 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0043 | 0.0094 | 0.0005 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0093 | 0.0005 | 0.0001 |
Cell Periphery | 0.001 | 0.0182 | 0.0024 | 0.0004 | 0.005 | 0.0014 | 0.002 | 0.0027 | 0.0043 | 0.0064 | 0.0013 | 0.0006 | 0.0007 | 0.0062 | 0.0071 | 0.0063 | 0.001 | 0.0007 |
Cytoplasm | 0.0109 | 0.0421 | 0.0279 | 0.0245 | 0.0273 | 0.0062 | 0.008 | 0.013 | 0.0134 | 0.0302 | 0.0024 | 0.0063 | 0.0037 | 0.0307 | 0.002 | 0.016 | 0.0034 | 0.0058 |
Cytoplasmic Foci | 0.0018 | 0.0009 | 0.0006 | 0.0001 | 0.0027 | 0.0006 | 0.0076 | 0.0002 | 0.0049 | 0.0023 | 0.0075 | 0.0025 | 0.0188 | 0.0001 | 0.003 | 0.0023 | 0.0005 | 0.0026 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.7786 | 0.7924 | 0.9007 | 0.9263 | 0.794 | 0.8384 | 0.7437 | 0.9277 | 0.8309 | 0.8328 | 0.7317 | 0.8199 | 0.7732 | 0.8804 | 0.9176 | 0.8246 | 0.7173 | 0.8287 |
Endosome | 0.0043 | 0.0025 | 0.002 | 0.0004 | 0.0085 | 0.001 | 0.0109 | 0.0009 | 0.0133 | 0.0159 | 0.0332 | 0.0074 | 0.0063 | 0.0005 | 0.0049 | 0.0082 | 0.0094 | 0.0065 |
Golgi | 0.0006 | 0.0003 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0039 | 0.0001 | 0.0063 | 0.0052 | 0.0232 | 0.0022 | 0.0033 | 0.0001 | 0.002 | 0.0027 | 0.0039 | 0.0008 |
Lipid Particles | 0.0046 | 0.0132 | 0.0019 | 0.0004 | 0.0062 | 0.0013 | 0.0101 | 0.0014 | 0.0073 | 0.0029 | 0.0401 | 0.0003 | 0.0151 | 0.0044 | 0.0031 | 0.0027 | 0.0029 | 0.0008 |
Mitochondria | 0.0006 | 0.0002 | 0.0011 | 0 | 0.0017 | 0.0001 | 0.0024 | 0.0001 | 0.0022 | 0.0014 | 0.0049 | 0.002 | 0.014 | 0 | 0.0039 | 0.0006 | 0.0289 | 0.0015 |
None | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0039 | 0.0003 | 0.0116 | 0.0001 | 0.011 | 0.0012 | 0.0006 | 0.0023 | 0.0049 | 0 | 0.0002 | 0.0004 | 0.0007 | 0.0005 |
Nuclear Periphery | 0.1565 | 0.0757 | 0.0444 | 0.0411 | 0.0924 | 0.1276 | 0.1486 | 0.0331 | 0.0553 | 0.0381 | 0.115 | 0.1443 | 0.1276 | 0.0608 | 0.0322 | 0.0786 | 0.1771 | 0.1259 |
Nucleolus | 0.0006 | 0.0007 | 0.0001 | 0 | 0.0056 | 0.0009 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0001 |
Nucleus | 0.0168 | 0.0181 | 0.0049 | 0.0031 | 0.0145 | 0.0089 | 0.008 | 0.0011 | 0.003 | 0.0011 | 0.0032 | 0.0017 | 0.0075 | 0.0025 | 0.001 | 0.0033 | 0.0111 | 0.0072 |
Peroxisomes | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0021 | 0 | 0.0007 | 0.0003 | 0.0019 | 0.0005 | 0.0068 | 0 | 0.0028 | 0.0002 | 0.0006 | 0.0003 |
Punctate Nuclear | 0.0031 | 0.0004 | 0.0007 | 0.0001 | 0.0081 | 0.0006 | 0.0033 | 0.0001 | 0.0008 | 0.0004 | 0.0013 | 0.0001 | 0.0021 | 0.0001 | 0.0077 | 0.0005 | 0.0009 | 0.0009 |
Vacuole | 0.0157 | 0.0296 | 0.0096 | 0.0028 | 0.0216 | 0.0095 | 0.0095 | 0.017 | 0.0162 | 0.004 | 0.014 | 0.005 | 0.0048 | 0.0122 | 0.0096 | 0.017 | 0.0105 | 0.0126 |
Vacuole Periphery | 0.0035 | 0.0012 | 0.0014 | 0.0003 | 0.0046 | 0.0018 | 0.0037 | 0.0018 | 0.0064 | 0.0044 | 0.0053 | 0.0035 | 0.0032 | 0.0011 | 0.0012 | 0.0047 | 0.0112 | 0.0033 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 114.7162 | 65.547 | 54.9146 | 105.9843 | 109.6343 | 128.8366 | 80.438 | 84.7721 | 99.422 | 117.0106 |
Translational Efficiency | 1.1723 | 1.3127 | 1.2236 | 1.0257 | 1.0972 | 1.1973 | 1.1255 | 0.9151 | 0.9912 | 0.9191 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
362 | 2656 | 425 | 1158 | 2040 | 1819 | 1473 | 1522 | 2402 | 4475 | 1898 | 2680 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1607.79 | 1489.53 | 1370.26 | 1873.22 | 1085.55 | 1641.88 | 1799.32 | 1830.15 | 1164.26 | 1551.46 | 1703.24 | 1848.76 |
Standard Deviation | 332.43 | 334.97 | 304.27 | 546.15 | 233.25 | 354.87 | 423.44 | 521.79 | 312.68 | 351.26 | 438.03 | 532.88 |
Intensity Change Log 2 | — | -0.110222 | -0.230629 | 0.220441 | — | 0.596923 | 0.729026 | 0.753536 | — | 0.217416 | 0.234895 | 0.459442 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000169 | 0.000275 | 0.001822 | 0.000736 | 0.000990 | 0.000464 | 0.000182 | 0.000652 | 0.000867 | 0.000352 | 0.000549 | 0.000688 |
Bud Neck | 0.002944 | 0.000127 | 0.004718 | 0.005773 | 0.000724 | 0.000482 | 0.000926 | 0.002210 | 0.001059 | 0.000271 | 0.001775 | 0.003750 |
Bud Site | 0.001585 | 0.000674 | 0.004802 | 0.010621 | 0.006007 | 0.002184 | 0.000817 | 0.006577 | 0.005340 | 0.001288 | 0.001709 | 0.008324 |
Cell Periphery | 0.002441 | 0.000684 | 0.000767 | 0.007673 | 0.001168 | 0.002432 | 0.001384 | 0.006821 | 0.001360 | 0.001394 | 0.001246 | 0.007189 |
Cytoplasm | 0.002238 | 0.008150 | 0.021811 | 0.009439 | 0.028400 | 0.004818 | 0.002589 | 0.010416 | 0.024457 | 0.006795 | 0.006893 | 0.009994 |
Cytoplasmic Foci | 0.000681 | 0.000630 | 0.003451 | 0.001985 | 0.011594 | 0.000862 | 0.000158 | 0.001745 | 0.009949 | 0.000724 | 0.000895 | 0.001849 |
Eisosomes | 0.000051 | 0.000012 | 0.000068 | 0.000097 | 0.000229 | 0.000068 | 0.000017 | 0.000039 | 0.000202 | 0.000035 | 0.000029 | 0.000064 |
Endoplasmic Reticulum | 0.858530* | 0.950040* | 0.584974* | 0.839789* | 0.854605* | 0.941037* | 0.966370* | 0.883461* | 0.855197* | 0.946381* | 0.880968* | 0.864591* |
Endosome | 0.012478 | 0.008107 | 0.022607 | 0.020639 | 0.015333 | 0.009155 | 0.005281 | 0.020512 | 0.014902 | 0.008533 | 0.009160 | 0.020567 |
Golgi | 0.002106 | 0.001250 | 0.000835 | 0.003756 | 0.005379 | 0.001394 | 0.000984 | 0.002952 | 0.004886 | 0.001309 | 0.000951 | 0.003300 |
Lipid Particles | 0.001215 | 0.001411 | 0.009337 | 0.011746 | 0.008053 | 0.002052 | 0.001083 | 0.007808 | 0.007022 | 0.001672 | 0.002931 | 0.009510 |
Mitochondria | 0.000333 | 0.000674 | 0.000719 | 0.002509 | 0.002712 | 0.000696 | 0.000347 | 0.001446 | 0.002353 | 0.000683 | 0.000430 | 0.001905 |
Mitotic Spindle | 0.000822 | 0.000090 | 0.005245 | 0.001375 | 0.000121 | 0.000189 | 0.000028 | 0.000534 | 0.000227 | 0.000130 | 0.001196 | 0.000897 |
None | 0.000039 | 0.002813 | 0.000366 | 0.002171 | 0.004715 | 0.001406 | 0.000373 | 0.002484 | 0.004010 | 0.002241 | 0.000371 | 0.002349 |
Nuclear Periphery | 0.036846 | 0.006989 | 0.155225* | 0.010196 | 0.014534 | 0.004907 | 0.006620 | 0.004715 | 0.017897 | 0.006143 | 0.039896 | 0.007083 |
Nuclear Periphery Foci | 0.027656 | 0.012442 | 0.152985 | 0.036384 | 0.026593 | 0.009591 | 0.006789 | 0.024108 | 0.026753 | 0.011283 | 0.039525 | 0.029412 |
Nucleolus | 0.000299 | 0.000013 | 0.000118 | 0.000099 | 0.000265 | 0.000023 | 0.000067 | 0.000043 | 0.000270 | 0.000017 | 0.000079 | 0.000067 |
Nucleus | 0.001694 | 0.000446 | 0.005218 | 0.001762 | 0.003306 | 0.000446 | 0.000923 | 0.001106 | 0.003063 | 0.000446 | 0.001885 | 0.001390 |
Peroxisomes | 0.000050 | 0.000045 | 0.000181 | 0.000113 | 0.000789 | 0.000053 | 0.000004 | 0.000029 | 0.000678 | 0.000048 | 0.000044 | 0.000065 |
Vacuole | 0.042193 | 0.004297 | 0.023546 | 0.028269 | 0.011711 | 0.016308 | 0.004562 | 0.019727 | 0.016304 | 0.009179 | 0.008813 | 0.023418 |
Vacuole Periphery | 0.005628 | 0.000830 | 0.001205 | 0.004871 | 0.002773 | 0.001433 | 0.000497 | 0.002615 | 0.003203 | 0.001075 | 0.000655 | 0.003590 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.64 | -2.00 | -3.91 | -3.27 | 1.12 | 1.81 | 2.93 | 0.30 | -1.90 | -3.49 | 2.17 | 0.87 | -0.20 | -3.66 | -1.29 |
Bud Neck | 2.33 | -1.61 | -2.40 | -7.12 | -0.56 | 1.02 | -0.76 | -4.95 | -6.07 | -3.15 | 3.15 | -2.25 | -6.50 | -9.13 | -3.77 |
Bud Site | 1.38 | -2.48 | -5.34 | -6.13 | -2.66 | 3.41 | 5.20 | -1.39 | -4.99 | -6.92 | 4.76 | 3.59 | -3.61 | -8.30 | -7.23 |
Cell Periphery | 1.18 | 1.09 | -3.38 | -9.48 | -9.05 | -2.94 | -1.05 | -7.01 | -5.00 | -6.54 | -0.16 | 0.16 | -9.73 | -10.12 | -10.24 |
Cytoplasm | -6.13 | -5.50 | -6.10 | -1.93 | 3.01 | 11.25 | 12.62 | 7.38 | -4.88 | -6.90 | 9.98 | 8.81 | 6.88 | -4.39 | -3.05 |
Cytoplasmic Foci | 0.30 | -3.12 | -2.14 | -2.36 | 1.48 | 8.52 | 9.17 | 7.42 | -2.29 | -4.24 | 8.66 | 8.19 | 7.05 | -3.43 | -2.26 |
Eisosomes | 0.99 | -0.31 | -1.05 | -3.67 | -0.58 | 1.11 | 1.48 | 1.31 | 1.07 | -3.86 | 1.37 | 1.42 | 1.11 | -2.16 | -2.49 |
Endoplasmic Reticulum | -6.81 | 13.19 | 2.03 | 14.59 | -13.48 | -12.70 | -17.15 | -2.48 | 9.46 | 13.64 | -16.31 | -2.87 | 0.27 | 17.50 | 3.19 |
Endosome | 2.83 | -4.43 | -4.64 | -10.56 | 0.86 | 6.11 | 10.68 | -4.35 | -10.06 | -13.95 | 7.64 | 5.94 | -5.88 | -14.72 | -12.41 |
Golgi | 0.80 | 1.20 | -1.36 | -3.36 | -4.28 | 5.17 | 5.76 | 2.94 | -2.80 | -3.57 | 5.50 | 5.98 | 2.19 | -4.40 | -5.07 |
Lipid Particles | -0.46 | -3.64 | -6.82 | -6.79 | -0.76 | 6.57 | 7.73 | -0.26 | -5.92 | -6.88 | 7.27 | 3.96 | -2.86 | -9.18 | -6.33 |
Mitochondria | -2.09 | -2.27 | -2.74 | -2.36 | -2.24 | 4.18 | 5.35 | 2.17 | -2.38 | -3.88 | 4.42 | 5.10 | 0.56 | -3.30 | -3.89 |
Mitotic Spindle | 1.31 | -2.76 | -0.93 | -3.69 | 2.50 | -0.75 | 2.88 | -3.13 | -2.57 | -3.64 | 1.04 | -2.77 | -3.77 | -4.62 | 0.90 |
None | -5.35 | -3.03 | -3.29 | 0.70 | -2.74 | 3.62 | 5.62 | 2.35 | -1.59 | -4.12 | 2.37 | 5.54 | 2.09 | -0.31 | -4.83 |
Nuclear Periphery | 5.07 | -9.71 | 4.45 | -2.56 | 13.64 | 6.15 | 4.80 | 6.53 | 0.10 | 2.06 | 7.42 | -6.79 | 6.67 | -1.47 | 11.36 |
Nuclear Periphery Foci | 4.05 | -13.25 | -2.06 | -8.07 | 12.56 | 7.43 | 9.35 | 0.70 | -6.67 | -8.55 | 8.32 | -4.31 | -1.47 | -10.36 | 3.16 |
Nucleolus | 1.27 | 0.82 | 0.90 | -3.01 | 0.41 | 2.47 | 1.74 | 2.23 | -2.40 | 0.57 | 2.83 | 1.90 | 2.20 | -3.86 | 0.34 |
Nucleus | 0.95 | -2.16 | -0.38 | -3.78 | 2.91 | 5.51 | 3.33 | 3.68 | -4.14 | -0.48 | 5.36 | 1.41 | 2.22 | -5.24 | 0.68 |
Peroxisomes | 0.30 | -1.61 | -0.64 | -0.83 | 0.75 | 5.41 | 5.81 | 5.61 | 1.37 | -5.37 | 5.47 | 5.41 | 5.09 | -0.48 | -0.44 |
Vacuole | 5.98 | 2.27 | 1.53 | -10.39 | -1.37 | -2.79 | 5.19 | -4.99 | -1.89 | -8.41 | 4.48 | 4.06 | -5.01 | -9.69 | -9.20 |
Vacuole Periphery | 4.02 | 3.70 | 0.54 | -5.32 | -4.81 | 2.95 | 5.93 | 0.16 | -2.63 | -5.34 | 5.56 | 6.86 | -0.85 | -6.15 | -7.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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