Standard name
Human Ortholog
Description Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0.07 0.12 0.06 0.08 0.1 0.08 0.13 0.07 0 0 0.07 0 0.12 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0.12 0.19 0.12 0.12 0.18 0 0 0 0 0 0
Cytoplasm 0.27 0.09 0.08 0.17 0.18 0.18 0.16 0.15 0.19 0.16 0.2 0.21 0.07 0.16 0.12 0.35 0.47 0.36 0 0 0 0 0 0
Endoplasmic Reticulum 0.59 0.77 0.84 0.71 0.63 0.57 0.63 0.6 0.54 0.56 0.57 0.49 0.87 0.77 0.72 0.05 0 0 0.8 0.92 0.91 0.89 0.84 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.17 0.18 0.18 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0.05 0.05 0.08 0.11 0 0 0 0.1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.09 0.06 0 0.06 0.1 0.1 0.05 0.1 0.1 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.2 0.1 0.12 0.21 0.16 0.14 0.13 0.14 0.21 0.07 0.12 0.07 0.07 0.1 0.22 0.18 0.23 0 0 0 0 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 8 11 20 23 43 23 31 28 41 33 24 8 7 20 1 2 2 3 8 2 2 1 1
Bud 0 0 0 2 3 6 8 16 13 10 2 13 0 1 0 0 0 0 0 4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cell Periphery 1 10 10 6 10 10 7 15 13 15 9 25 11 25 54 5 2 4 0 0 0 0 0 0
Cytoplasm 14 52 26 50 63 64 62 58 55 83 53 69 21 34 34 14 8 8 1 6 1 2 3 3
Endoplasmic Reticulum 30 463 289 210 218 204 241 235 156 297 148 158 244 165 205 2 0 0 73 630 352 191 173 228
Endosome 0 0 3 2 1 2 2 0 2 0 0 2 0 1 2 0 0 0 2 0 2 1 1 2
Golgi 0 13 4 21 5 1 1 1 0 2 0 0 8 3 6 7 3 4 0 3 1 1 2 4
Mitochondria 0 0 0 1 2 6 15 23 14 25 22 35 0 0 0 4 0 0 0 1 0 0 1 2
Nucleus 0 0 0 0 1 2 1 2 4 2 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 2 6 32 17 15 22 37 37 15 53 27 27 13 3 9 0 0 0 0 10 10 4 4 4
Nucleolus 2 2 1 0 1 1 0 4 4 2 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 120 36 36 73 57 54 50 41 110 17 40 21 15 28 9 3 5 2 17 12 9 13 31
Unique Cell Count 51 601 343 296 344 356 382 389 287 532 260 321 282 214 286 40 17 22 91 688 388 215 205 282
Labelled Cell Count 57 675 412 365 415 418 451 472 346 640 315 398 326 254 358 43 18 23 91 688 388 215 205 282


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.6 26.6 16.6 15.3 14.0 13.1 11.6 11.5 11.8 11.0 11.2 11.1 20.1 18.4 18.3 25.7 27.8 26.0 28.6 24.8 23.9
Std Deviation (1e-4) 19.5 6.9 4.4 3.7 3.7 3.1 2.8 2.6 2.8 2.8 2.7 2.6 4.8 4.9 4.8 7.1 7.5 7.7 7.3 6.8 5.6
Intensity Change (Log2) -0.11 -0.24 -0.34 -0.52 -0.53 -0.5 -0.59 -0.57 -0.58 0.28 0.15 0.14 0.63 0.74 0.65 0.78 0.58 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.1 2.1 4.4 1.8 2.8 3.4 2.7 4.4 2.5 -0.3 0 2.2 0 0 0
Bud 0 0 0 0 3.8 4.0 0 0 3.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 0 -0.1 -1.0 0.7 1.1 -0.1 0.4 2.8 0.7 4.1 6.6 0 0 0
Cytoplasm 3.6 4.2 4.1 3.6 3.1 4.3 3.5 4.6 5.1 -0.1 3.1 1.8 0 0 0
Endoplasmic Reticulum -4.1 -6.2 -7.8 -6.4 -7.1 -8.2 -8.7 -7.4 -9.6 0.8 -2.1 -3.8 -11.1 -8.5 -9.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.9 0 0 0 0 0 0 0 0 1.5 0 0 0 0 0
Mitochondria 0 0 0 3.7 4.6 4.1 4.1 5.5 6.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.7 -2.6 -1.6 0.2 0.1 -2.0 0.3 0.4 -0.4 -2.3 -3.8 -3.1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 3.8 2.1 1.5 1.0 1.4 3.9 -1.7 0.8 -1.3 -1.4 -0.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.395 25.3188 23.3264 23.5232 21.3407 23.8351 25.2477 32.367 26.7378 23.0871 25.2464 31.0968 23.4826 25.1357 24.756 23.2964 24.3227 25.9997
Actin 0.0003 0.0003 0.0001 0.0001 0.0012 0.0001 0.0183 0.0002 0.0166 0.0194 0.0132 0.0004 0.0059 0.0001 0.0013 0.0169 0.0165 0.001
Bud 0 0.0007 0 0 0.0001 0 0.0005 0.0001 0.0005 0.0038 0.0001 0.0001 0.0001 0 0 0.0012 0.0004 0
Bud Neck 0.0002 0.0005 0.0003 0.0002 0.0006 0.0011 0.0002 0.0001 0.0005 0.0003 0.0002 0.0004 0.0002 0.0006 0.0001 0.0024 0.0006 0.0004
Bud Periphery 0.0001 0.0006 0.0001 0 0.0001 0.0001 0.0036 0.0004 0.0014 0.0202 0.0002 0.0003 0.0002 0.0001 0.0001 0.0018 0.0015 0.0001
Bud Site 0.0003 0.002 0.0006 0 0.0002 0.0001 0.0012 0 0.0043 0.0094 0.0005 0.0001 0.0007 0 0.0001 0.0093 0.0005 0.0001
Cell Periphery 0.001 0.0182 0.0024 0.0004 0.005 0.0014 0.002 0.0027 0.0043 0.0064 0.0013 0.0006 0.0007 0.0062 0.0071 0.0063 0.001 0.0007
Cytoplasm 0.0109 0.0421 0.0279 0.0245 0.0273 0.0062 0.008 0.013 0.0134 0.0302 0.0024 0.0063 0.0037 0.0307 0.002 0.016 0.0034 0.0058
Cytoplasmic Foci 0.0018 0.0009 0.0006 0.0001 0.0027 0.0006 0.0076 0.0002 0.0049 0.0023 0.0075 0.0025 0.0188 0.0001 0.003 0.0023 0.0005 0.0026
Eisosomes 0 0 0 0 0.0003 0 0.0006 0 0.0002 0.0001 0.0001 0 0.0002 0 0 0.0001 0.0003 0
Endoplasmic Reticulum 0.7786 0.7924 0.9007 0.9263 0.794 0.8384 0.7437 0.9277 0.8309 0.8328 0.7317 0.8199 0.7732 0.8804 0.9176 0.8246 0.7173 0.8287
Endosome 0.0043 0.0025 0.002 0.0004 0.0085 0.001 0.0109 0.0009 0.0133 0.0159 0.0332 0.0074 0.0063 0.0005 0.0049 0.0082 0.0094 0.0065
Golgi 0.0006 0.0003 0.0002 0 0.0012 0.0001 0.0039 0.0001 0.0063 0.0052 0.0232 0.0022 0.0033 0.0001 0.002 0.0027 0.0039 0.0008
Lipid Particles 0.0046 0.0132 0.0019 0.0004 0.0062 0.0013 0.0101 0.0014 0.0073 0.0029 0.0401 0.0003 0.0151 0.0044 0.0031 0.0027 0.0029 0.0008
Mitochondria 0.0006 0.0002 0.0011 0 0.0017 0.0001 0.0024 0.0001 0.0022 0.0014 0.0049 0.002 0.014 0 0.0039 0.0006 0.0289 0.0015
None 0.0004 0.0002 0.0006 0.0001 0.0039 0.0003 0.0116 0.0001 0.011 0.0012 0.0006 0.0023 0.0049 0 0.0002 0.0004 0.0007 0.0005
Nuclear Periphery 0.1565 0.0757 0.0444 0.0411 0.0924 0.1276 0.1486 0.0331 0.0553 0.0381 0.115 0.1443 0.1276 0.0608 0.0322 0.0786 0.1771 0.1259
Nucleolus 0.0006 0.0007 0.0001 0 0.0056 0.0009 0.0002 0 0.0003 0 0.0001 0 0.0007 0 0.0001 0.0002 0.0006 0.0001
Nucleus 0.0168 0.0181 0.0049 0.0031 0.0145 0.0089 0.008 0.0011 0.003 0.0011 0.0032 0.0017 0.0075 0.0025 0.001 0.0033 0.0111 0.0072
Peroxisomes 0.0002 0 0.0001 0 0.0003 0 0.0021 0 0.0007 0.0003 0.0019 0.0005 0.0068 0 0.0028 0.0002 0.0006 0.0003
Punctate Nuclear 0.0031 0.0004 0.0007 0.0001 0.0081 0.0006 0.0033 0.0001 0.0008 0.0004 0.0013 0.0001 0.0021 0.0001 0.0077 0.0005 0.0009 0.0009
Vacuole 0.0157 0.0296 0.0096 0.0028 0.0216 0.0095 0.0095 0.017 0.0162 0.004 0.014 0.005 0.0048 0.0122 0.0096 0.017 0.0105 0.0126
Vacuole Periphery 0.0035 0.0012 0.0014 0.0003 0.0046 0.0018 0.0037 0.0018 0.0064 0.0044 0.0053 0.0035 0.0032 0.0011 0.0012 0.0047 0.0112 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.7162 65.547 54.9146 105.9843 109.6343 128.8366 80.438 84.7721 99.422 117.0106
Translational Efficiency 1.1723 1.3127 1.2236 1.0257 1.0972 1.1973 1.1255 0.9151 0.9912 0.9191

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Elo1

Elo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available