Standard name
Human Ortholog
Description Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication
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Mutation Strain ID Temperature Mean Single Mutant Fitness Single Mutant Fitness SD Penetrance Results Specific Phenotype Results
elo1Δ DMA2573 30 1.032 0.0678
compartment (FP-tagged protein)# cells# replicatesmean penetrancepenetrance SDpenetrance mutantimage
Actin patch (Sac6)96117.7—no
Cortical patch (Sla1)97113.4—no
Late endosome (Snf7)58127.6—no
Vacuole (Vph1)70122.9—no
phenotypemeanSDSPMdiagram
Actin patchActin patch
Actin patch (Sac6)aggregate2.1—no
Actin patch (Sac6)bright patches1.0—no
Actin patch (Sac6)decreased patch number6.2—no
Actin patch (Sac6)depolarized patches8.3—no
Actin patch (Sac6)WT82.4NANA
Cortical patchCortical patch
Cortical patch (Sla1)aggregate3.1—no
Cortical patch (Sla1)decreased patch number4.1—no
Cortical patch (Sla1)depolarized patches2.1—no
Cortical patch (Sla1)increased patch number3.1—no
Cortical patch (Sla1)WT86.6NANA
Late endosomeLate endosome
Late endosome (Snf7)fragmented1.7—no
Late endosome (Snf7)condensed17.2—no
Late endosome (Snf7)membrane **6.9—no
Late endosome (Snf7)WT72.5NANA
VacuoleVacuole
Vacuole (Vph1)class E10.0—no
Vacuole (Vph1)enlarged1.4—no
Vacuole (Vph1)fragmented5.7—no
Vacuole (Vph1)multilobed2.9—no
Vacuole (Vph1)class G2.9—no
Vacuole (Vph1)V-ATPase defect0—no
Vacuole (Vph1)WT77.1NANA
penetrance = percentage of the population with an aberrant phenotype

FP = fluorescent protein

SD = standard deviation

* temperature-sentitive strains imaged at room temperature (RT) were analyzed but not scored

specific phenotype mutant (SPM)
  • yes+ = stringent SPM

** membrane = localization of Snf7 to the vacuolar membrane

NA = not applicable

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0.07 0.12 0.06 0.08 0.1 0.08 0.13 0.07 0 0 0.07 0 0.12 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0.12 0.19 0.12 0.12 0.18 0 0 0 0 0 0
Cytoplasm 0.27 0.09 0.08 0.17 0.18 0.18 0.16 0.15 0.19 0.16 0.2 0.21 0.07 0.16 0.12 0.35 0.47 0.36 0 0 0 0 0 0
Endoplasmic Reticulum 0.59 0.77 0.84 0.71 0.63 0.57 0.63 0.6 0.54 0.56 0.57 0.49 0.87 0.77 0.72 0.05 0 0 0.8 0.92 0.91 0.89 0.84 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.17 0.18 0.18 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0.05 0.05 0.08 0.11 0 0 0 0.1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.09 0.06 0 0.06 0.1 0.1 0.05 0.1 0.1 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.2 0.1 0.12 0.21 0.16 0.14 0.13 0.14 0.21 0.07 0.12 0.07 0.07 0.1 0.22 0.18 0.23 0 0 0 0 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 8 11 20 23 43 23 31 28 41 33 24 8 7 20 1 2 2 3 8 2 2 1 1
Bud 0 0 0 2 3 6 8 16 13 10 2 13 0 1 0 0 0 0 0 4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 10 10 6 10 10 7 15 13 15 9 25 11 25 54 5 2 4 0 0 0 0 0 0
Cytoplasm 14 52 26 50 63 64 62 58 55 83 53 69 21 34 34 14 8 8 1 6 1 2 3 3
Endoplasmic Reticulum 30 463 289 210 218 204 241 235 156 297 148 158 244 165 205 2 0 0 73 630 352 191 173 228
Endosome 0 0 3 2 1 2 2 0 2 0 0 2 0 1 2 0 0 0 2 0 2 1 1 2
Golgi 0 13 4 21 5 1 1 1 0 2 0 0 8 3 6 7 3 4 0 3 1 1 2 4
Mitochondria 0 0 0 1 2 6 15 23 14 25 22 35 0 0 0 4 0 0 0 1 0 0 1 2
Nucleus 0 0 0 0 1 2 1 2 4 2 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 2 6 32 17 15 22 37 37 15 53 27 27 13 3 9 0 0 0 0 10 10 4 4 4
Nucleolus 2 2 1 0 1 1 0 4 4 2 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 120 36 36 73 57 54 50 41 110 17 40 21 15 28 9 3 5 2 17 12 9 13 31
Unique Cell Count 51 601 343 296 344 356 382 389 287 532 260 321 282 214 286 40 17 22 91 688 388 215 205 282
Labelled Cell Count 57 675 412 365 415 418 451 472 346 640 315 398 326 254 358 43 18 23 91 688 388 215 205 282


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.6 26.6 16.6 15.3 14.0 13.1 11.6 11.5 11.8 11.0 11.2 11.1 20.1 18.4 18.3 25.7 27.8 26.0 28.6 24.8 23.9
Std Deviation (1e-4) 19.5 6.9 4.4 3.7 3.7 3.1 2.8 2.6 2.8 2.8 2.7 2.6 4.8 4.9 4.8 7.1 7.5 7.7 7.3 6.8 5.6
Intensity Change (Log2) – – – -0.11 -0.24 -0.34 -0.52 -0.53 -0.5 -0.59 -0.57 -0.58 0.28 0.15 0.14 0.63 0.74 0.65 0.78 0.58 0.52

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002040WT3HU80HU120HU16002040WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302040WT1AF100AF140AF18002040
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.1 2.1 4.4 1.8 2.8 3.4 2.7 4.4 2.5 -0.3 0 2.2 0 0 0
Bud 0 0 0 0 3.8 4.0 0 0 3.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 0 -0.1 -1.0 0.7 1.1 -0.1 0.4 2.8 0.7 4.1 6.6 0 0 0
Cytoplasm 3.6 4.2 4.1 3.6 3.1 4.3 3.5 4.6 5.1 -0.1 3.1 1.8 0 0 0
Endoplasmic Reticulum -4.1 -6.2 -7.8 -6.4 -7.1 -8.2 -8.7 -7.4 -9.6 0.8 -2.1 -3.8 -11.1 -8.5 -9.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.9 0 0 0 0 0 0 0 0 1.5 0 0 0 0 0
Mitochondria 0 0 0 3.7 4.6 4.1 4.1 5.5 6.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.7 -2.6 -1.6 0.2 0.1 -2.0 0.3 0.4 -0.4 -2.3 -3.8 -3.1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 3.8 2.1 1.5 1.0 1.4 3.9 -1.7 0.8 -1.3 -1.4 -0.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.395 25.3188 23.3264 23.5232 21.3407 23.8351 25.2477 32.367 26.7378 23.0871 25.2464 31.0968 23.4826 25.1357 24.756 23.2964 24.3227 25.9997
Actin 0.0003 0.0003 0.0001 0.0001 0.0012 0.0001 0.0183 0.0002 0.0166 0.0194 0.0132 0.0004 0.0059 0.0001 0.0013 0.0169 0.0165 0.001
Bud 0 0.0007 0 0 0.0001 0 0.0005 0.0001 0.0005 0.0038 0.0001 0.0001 0.0001 0 0 0.0012 0.0004 0
Bud Neck 0.0002 0.0005 0.0003 0.0002 0.0006 0.0011 0.0002 0.0001 0.0005 0.0003 0.0002 0.0004 0.0002 0.0006 0.0001 0.0024 0.0006 0.0004
Bud Periphery 0.0001 0.0006 0.0001 0 0.0001 0.0001 0.0036 0.0004 0.0014 0.0202 0.0002 0.0003 0.0002 0.0001 0.0001 0.0018 0.0015 0.0001
Bud Site 0.0003 0.002 0.0006 0 0.0002 0.0001 0.0012 0 0.0043 0.0094 0.0005 0.0001 0.0007 0 0.0001 0.0093 0.0005 0.0001
Cell Periphery 0.001 0.0182 0.0024 0.0004 0.005 0.0014 0.002 0.0027 0.0043 0.0064 0.0013 0.0006 0.0007 0.0062 0.0071 0.0063 0.001 0.0007
Cytoplasm 0.0109 0.0421 0.0279 0.0245 0.0273 0.0062 0.008 0.013 0.0134 0.0302 0.0024 0.0063 0.0037 0.0307 0.002 0.016 0.0034 0.0058
Cytoplasmic Foci 0.0018 0.0009 0.0006 0.0001 0.0027 0.0006 0.0076 0.0002 0.0049 0.0023 0.0075 0.0025 0.0188 0.0001 0.003 0.0023 0.0005 0.0026
Eisosomes 0 0 0 0 0.0003 0 0.0006 0 0.0002 0.0001 0.0001 0 0.0002 0 0 0.0001 0.0003 0
Endoplasmic Reticulum 0.7786 0.7924 0.9007 0.9263 0.794 0.8384 0.7437 0.9277 0.8309 0.8328 0.7317 0.8199 0.7732 0.8804 0.9176 0.8246 0.7173 0.8287
Endosome 0.0043 0.0025 0.002 0.0004 0.0085 0.001 0.0109 0.0009 0.0133 0.0159 0.0332 0.0074 0.0063 0.0005 0.0049 0.0082 0.0094 0.0065
Golgi 0.0006 0.0003 0.0002 0 0.0012 0.0001 0.0039 0.0001 0.0063 0.0052 0.0232 0.0022 0.0033 0.0001 0.002 0.0027 0.0039 0.0008
Lipid Particles 0.0046 0.0132 0.0019 0.0004 0.0062 0.0013 0.0101 0.0014 0.0073 0.0029 0.0401 0.0003 0.0151 0.0044 0.0031 0.0027 0.0029 0.0008
Mitochondria 0.0006 0.0002 0.0011 0 0.0017 0.0001 0.0024 0.0001 0.0022 0.0014 0.0049 0.002 0.014 0 0.0039 0.0006 0.0289 0.0015
None 0.0004 0.0002 0.0006 0.0001 0.0039 0.0003 0.0116 0.0001 0.011 0.0012 0.0006 0.0023 0.0049 0 0.0002 0.0004 0.0007 0.0005
Nuclear Periphery 0.1565 0.0757 0.0444 0.0411 0.0924 0.1276 0.1486 0.0331 0.0553 0.0381 0.115 0.1443 0.1276 0.0608 0.0322 0.0786 0.1771 0.1259
Nucleolus 0.0006 0.0007 0.0001 0 0.0056 0.0009 0.0002 0 0.0003 0 0.0001 0 0.0007 0 0.0001 0.0002 0.0006 0.0001
Nucleus 0.0168 0.0181 0.0049 0.0031 0.0145 0.0089 0.008 0.0011 0.003 0.0011 0.0032 0.0017 0.0075 0.0025 0.001 0.0033 0.0111 0.0072
Peroxisomes 0.0002 0 0.0001 0 0.0003 0 0.0021 0 0.0007 0.0003 0.0019 0.0005 0.0068 0 0.0028 0.0002 0.0006 0.0003
Punctate Nuclear 0.0031 0.0004 0.0007 0.0001 0.0081 0.0006 0.0033 0.0001 0.0008 0.0004 0.0013 0.0001 0.0021 0.0001 0.0077 0.0005 0.0009 0.0009
Vacuole 0.0157 0.0296 0.0096 0.0028 0.0216 0.0095 0.0095 0.017 0.0162 0.004 0.014 0.005 0.0048 0.0122 0.0096 0.017 0.0105 0.0126
Vacuole Periphery 0.0035 0.0012 0.0014 0.0003 0.0046 0.0018 0.0037 0.0018 0.0064 0.0044 0.0053 0.0035 0.0032 0.0011 0.0012 0.0047 0.0112 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.7162 65.547 54.9146 105.9843 109.6343 128.8366 80.438 84.7721 99.422 117.0106
Translational Efficiency 1.1723 1.3127 1.2236 1.0257 1.0972 1.1973 1.1255 0.9151 0.9912 0.9191

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
362 2656 425 1158 2040 1819 1473 1522 2402 4475 1898 2680

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1607.79 1489.53 1370.26 1873.22 1085.55 1641.88 1799.32 1830.15 1164.26 1551.46 1703.24 1848.76
Standard Deviation 332.43 334.97 304.27 546.15 233.25 354.87 423.44 521.79 312.68 351.26 438.03 532.88
Intensity Change Log 2 — -0.110222 -0.230629 0.220441 — 0.596923 0.729026 0.753536 — 0.217416 0.234895 0.459442

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000169 0.000275 0.001822 0.000736 0.000990 0.000464 0.000182 0.000652 0.000867 0.000352 0.000549 0.000688
Bud Neck 0.002944 0.000127 0.004718 0.005773 0.000724 0.000482 0.000926 0.002210 0.001059 0.000271 0.001775 0.003750
Bud Site 0.001585 0.000674 0.004802 0.010621 0.006007 0.002184 0.000817 0.006577 0.005340 0.001288 0.001709 0.008324
Cell Periphery 0.002441 0.000684 0.000767 0.007673 0.001168 0.002432 0.001384 0.006821 0.001360 0.001394 0.001246 0.007189
Cytoplasm 0.002238 0.008150 0.021811 0.009439 0.028400 0.004818 0.002589 0.010416 0.024457 0.006795 0.006893 0.009994
Cytoplasmic Foci 0.000681 0.000630 0.003451 0.001985 0.011594 0.000862 0.000158 0.001745 0.009949 0.000724 0.000895 0.001849
Eisosomes 0.000051 0.000012 0.000068 0.000097 0.000229 0.000068 0.000017 0.000039 0.000202 0.000035 0.000029 0.000064
Endoplasmic Reticulum 0.858530 0.950040 0.584974 0.839789 0.854605 0.941037 0.966370 0.883461 0.855197 0.946381 0.880968 0.864591
Endosome 0.012478 0.008107 0.022607 0.020639 0.015333 0.009155 0.005281 0.020512 0.014902 0.008533 0.009160 0.020567
Golgi 0.002106 0.001250 0.000835 0.003756 0.005379 0.001394 0.000984 0.002952 0.004886 0.001309 0.000951 0.003300
Lipid Particles 0.001215 0.001411 0.009337 0.011746 0.008053 0.002052 0.001083 0.007808 0.007022 0.001672 0.002931 0.009510
Mitochondria 0.000333 0.000674 0.000719 0.002509 0.002712 0.000696 0.000347 0.001446 0.002353 0.000683 0.000430 0.001905
Mitotic Spindle 0.000822 0.000090 0.005245 0.001375 0.000121 0.000189 0.000028 0.000534 0.000227 0.000130 0.001196 0.000897
None 0.000039 0.002813 0.000366 0.002171 0.004715 0.001406 0.000373 0.002484 0.004010 0.002241 0.000371 0.002349
Nuclear Periphery 0.036846 0.006989 0.155225 0.010196 0.014534 0.004907 0.006620 0.004715 0.017897 0.006143 0.039896 0.007083
Nuclear Periphery Foci 0.027656 0.012442 0.152985 0.036384 0.026593 0.009591 0.006789 0.024108 0.026753 0.011283 0.039525 0.029412
Nucleolus 0.000299 0.000013 0.000118 0.000099 0.000265 0.000023 0.000067 0.000043 0.000270 0.000017 0.000079 0.000067
Nucleus 0.001694 0.000446 0.005218 0.001762 0.003306 0.000446 0.000923 0.001106 0.003063 0.000446 0.001885 0.001390
Peroxisomes 0.000050 0.000045 0.000181 0.000113 0.000789 0.000053 0.000004 0.000029 0.000678 0.000048 0.000044 0.000065
Vacuole 0.042193 0.004297 0.023546 0.028269 0.011711 0.016308 0.004562 0.019727 0.016304 0.009179 0.008813 0.023418
Vacuole Periphery 0.005628 0.000830 0.001205 0.004871 0.002773 0.001433 0.000497 0.002615 0.003203 0.001075 0.000655 0.003590

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.64 -2.00 -3.91 -3.27 1.12 1.81 2.93 0.30 -1.90 -3.49 2.17 0.87 -0.20 -3.66 -1.29
Bud Neck 2.33 -1.61 -2.40 -7.12 -0.56 1.02 -0.76 -4.95 -6.07 -3.15 3.15 -2.25 -6.50 -9.13 -3.77
Bud Site 1.38 -2.48 -5.34 -6.13 -2.66 3.41 5.20 -1.39 -4.99 -6.92 4.76 3.59 -3.61 -8.30 -7.23
Cell Periphery 1.18 1.09 -3.38 -9.48 -9.05 -2.94 -1.05 -7.01 -5.00 -6.54 -0.16 0.16 -9.73 -10.12 -10.24
Cytoplasm -6.13 -5.50 -6.10 -1.93 3.01 11.25 12.62 7.38 -4.88 -6.90 9.98 8.81 6.88 -4.39 -3.05
Cytoplasmic Foci 0.30 -3.12 -2.14 -2.36 1.48 8.52 9.17 7.42 -2.29 -4.24 8.66 8.19 7.05 -3.43 -2.26
Eisosomes 0.99 -0.31 -1.05 -3.67 -0.58 1.11 1.48 1.31 1.07 -3.86 1.37 1.42 1.11 -2.16 -2.49
Endoplasmic Reticulum -6.81 13.19 2.03 14.59 -13.48 -12.70 -17.15 -2.48 9.46 13.64 -16.31 -2.87 0.27 17.50 3.19
Endosome 2.83 -4.43 -4.64 -10.56 0.86 6.11 10.68 -4.35 -10.06 -13.95 7.64 5.94 -5.88 -14.72 -12.41
Golgi 0.80 1.20 -1.36 -3.36 -4.28 5.17 5.76 2.94 -2.80 -3.57 5.50 5.98 2.19 -4.40 -5.07
Lipid Particles -0.46 -3.64 -6.82 -6.79 -0.76 6.57 7.73 -0.26 -5.92 -6.88 7.27 3.96 -2.86 -9.18 -6.33
Mitochondria -2.09 -2.27 -2.74 -2.36 -2.24 4.18 5.35 2.17 -2.38 -3.88 4.42 5.10 0.56 -3.30 -3.89
Mitotic Spindle 1.31 -2.76 -0.93 -3.69 2.50 -0.75 2.88 -3.13 -2.57 -3.64 1.04 -2.77 -3.77 -4.62 0.90
None -5.35 -3.03 -3.29 0.70 -2.74 3.62 5.62 2.35 -1.59 -4.12 2.37 5.54 2.09 -0.31 -4.83
Nuclear Periphery 5.07 -9.71 4.45 -2.56 13.64 6.15 4.80 6.53 0.10 2.06 7.42 -6.79 6.67 -1.47 11.36
Nuclear Periphery Foci 4.05 -13.25 -2.06 -8.07 12.56 7.43 9.35 0.70 -6.67 -8.55 8.32 -4.31 -1.47 -10.36 3.16
Nucleolus 1.27 0.82 0.90 -3.01 0.41 2.47 1.74 2.23 -2.40 0.57 2.83 1.90 2.20 -3.86 0.34
Nucleus 0.95 -2.16 -0.38 -3.78 2.91 5.51 3.33 3.68 -4.14 -0.48 5.36 1.41 2.22 -5.24 0.68
Peroxisomes 0.30 -1.61 -0.64 -0.83 0.75 5.41 5.81 5.61 1.37 -5.37 5.47 5.41 5.09 -0.48 -0.44
Vacuole 5.98 2.27 1.53 -10.39 -1.37 -2.79 5.19 -4.99 -1.89 -8.41 4.48 4.06 -5.01 -9.69 -9.20
Vacuole Periphery 4.02 3.70 0.54 -5.32 -4.81 2.95 5.93 0.16 -2.63 -5.34 5.56 6.86 -0.85 -6.15 -7.32
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Elo1

Elo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available