Standard name | |
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Description | Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 8 | 11 | 20 | 23 | 43 | 23 | 31 | 28 | 41 | 33 | 24 | 8 | 7 | 20 | 1 | 2 | 2 | 3 | 8 | 2 | 2 | 1 | 1 |
Bud | 0 | 0 | 0 | 2 | 3 | 6 | 8 | 16 | 13 | 10 | 2 | 13 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 10 | 10 | 6 | 10 | 10 | 7 | 15 | 13 | 15 | 9 | 25 | 11 | 25 | 54 | 5 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 52 | 26 | 50 | 63 | 64 | 62 | 58 | 55 | 83 | 53 | 69 | 21 | 34 | 34 | 14 | 8 | 8 | 1 | 6 | 1 | 2 | 3 | 3 |
Endoplasmic Reticulum | 30 | 463 | 289 | 210 | 218 | 204 | 241 | 235 | 156 | 297 | 148 | 158 | 244 | 165 | 205 | 2 | 0 | 0 | 73 | 630 | 352 | 191 | 173 | 228 |
Endosome | 0 | 0 | 3 | 2 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 2 |
Golgi | 0 | 13 | 4 | 21 | 5 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 8 | 3 | 6 | 7 | 3 | 4 | 0 | 3 | 1 | 1 | 2 | 4 |
Mitochondria | 0 | 0 | 0 | 1 | 2 | 6 | 15 | 23 | 14 | 25 | 22 | 35 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 4 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 6 | 32 | 17 | 15 | 22 | 37 | 37 | 15 | 53 | 27 | 27 | 13 | 3 | 9 | 0 | 0 | 0 | 0 | 10 | 10 | 4 | 4 | 4 |
Nucleolus | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 4 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 120 | 36 | 36 | 73 | 57 | 54 | 50 | 41 | 110 | 17 | 40 | 21 | 15 | 28 | 9 | 3 | 5 | 2 | 17 | 12 | 9 | 13 | 31 |
Unique Cell Count | 51 | 601 | 343 | 296 | 344 | 356 | 382 | 389 | 287 | 532 | 260 | 321 | 282 | 214 | 286 | 40 | 17 | 22 | 91 | 688 | 388 | 215 | 205 | 282 |
Labelled Cell Count | 57 | 675 | 412 | 365 | 415 | 418 | 451 | 472 | 346 | 640 | 315 | 398 | 326 | 254 | 358 | 43 | 18 | 23 | 91 | 688 | 388 | 215 | 205 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 34.6 | 26.6 | 16.6 | 15.3 | 14.0 | 13.1 | 11.6 | 11.5 | 11.8 | 11.0 | 11.2 | 11.1 | 20.1 | 18.4 | 18.3 | 25.7 | 27.8 | 26.0 | 28.6 | 24.8 | 23.9 |
Std Deviation (1e-4) | 19.5 | 6.9 | 4.4 | 3.7 | 3.7 | 3.1 | 2.8 | 2.6 | 2.8 | 2.8 | 2.7 | 2.6 | 4.8 | 4.9 | 4.8 | 7.1 | 7.5 | 7.7 | 7.3 | 6.8 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.24 | -0.34 | -0.52 | -0.53 | -0.5 | -0.59 | -0.57 | -0.58 | 0.28 | 0.15 | 0.14 | 0.63 | 0.74 | 0.65 | 0.78 | 0.58 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.1 | 2.1 | 4.4 | 1.8 | 2.8 | 3.4 | 2.7 | 4.4 | 2.5 | -0.3 | 0 | 2.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3.8 | 4.0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | 0 | -0.1 | -1.0 | 0.7 | 1.1 | -0.1 | 0.4 | 2.8 | 0.7 | 4.1 | 6.6 | 0 | 0 | 0 |
Cytoplasm | 3.6 | 4.2 | 4.1 | 3.6 | 3.1 | 4.3 | 3.5 | 4.6 | 5.1 | -0.1 | 3.1 | 1.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | -4.1 | -6.2 | -7.8 | -6.4 | -7.1 | -8.2 | -8.7 | -7.4 | -9.6 | 0.8 | -2.1 | -3.8 | -11.1 | -8.5 | -9.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.7 | 4.6 | 4.1 | 4.1 | 5.5 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.7 | -2.6 | -1.6 | 0.2 | 0.1 | -2.0 | 0.3 | 0.4 | -0.4 | -2.3 | -3.8 | -3.1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.7 | 3.8 | 2.1 | 1.5 | 1.0 | 1.4 | 3.9 | -1.7 | 0.8 | -1.3 | -1.4 | -0.3 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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