Standard name
Description Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0.07 0.12 0.06 0.08 0.1 0.08 0.13 0.07 0 0 0.07 0 0.12 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0.12 0.19 0.12 0.12 0.18 0 0 0 0 0 0
Cytoplasm 0.27 0.09 0.08 0.17 0.18 0.18 0.16 0.15 0.19 0.16 0.2 0.21 0.07 0.16 0.12 0.35 0.47 0.36 0 0 0 0 0 0
Endoplasmic Reticulum 0.59 0.77 0.84 0.71 0.63 0.57 0.63 0.6 0.54 0.56 0.57 0.49 0.87 0.77 0.72 0.05 0 0 0.8 0.92 0.91 0.89 0.84 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.17 0.18 0.18 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0.05 0.05 0.08 0.11 0 0 0 0.1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.09 0.06 0 0.06 0.1 0.1 0.05 0.1 0.1 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.2 0.1 0.12 0.21 0.16 0.14 0.13 0.14 0.21 0.07 0.12 0.07 0.07 0.1 0.22 0.18 0.23 0 0 0 0 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 8 11 20 23 43 23 31 28 41 33 24 8 7 20 1 2 2 3 8 2 2 1 1
Bud 0 0 0 2 3 6 8 16 13 10 2 13 0 1 0 0 0 0 0 4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 10 10 6 10 10 7 15 13 15 9 25 11 25 54 5 2 4 0 0 0 0 0 0
Cytoplasm 14 52 26 50 63 64 62 58 55 83 53 69 21 34 34 14 8 8 1 6 1 2 3 3
Endoplasmic Reticulum 30 463 289 210 218 204 241 235 156 297 148 158 244 165 205 2 0 0 73 630 352 191 173 228
Endosome 0 0 3 2 1 2 2 0 2 0 0 2 0 1 2 0 0 0 2 0 2 1 1 2
Golgi 0 13 4 21 5 1 1 1 0 2 0 0 8 3 6 7 3 4 0 3 1 1 2 4
Mitochondria 0 0 0 1 2 6 15 23 14 25 22 35 0 0 0 4 0 0 0 1 0 0 1 2
Nucleus 0 0 0 0 1 2 1 2 4 2 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 2 6 32 17 15 22 37 37 15 53 27 27 13 3 9 0 0 0 0 10 10 4 4 4
Nucleolus 2 2 1 0 1 1 0 4 4 2 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 120 36 36 73 57 54 50 41 110 17 40 21 15 28 9 3 5 2 17 12 9 13 31
Unique Cell Count 51 601 343 296 344 356 382 389 287 532 260 321 282 214 286 40 17 22 91 688 388 215 205 282
Labelled Cell Count 57 675 412 365 415 418 451 472 346 640 315 398 326 254 358 43 18 23 91 688 388 215 205 282


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.6 26.6 16.6 15.3 14.0 13.1 11.6 11.5 11.8 11.0 11.2 11.1 20.1 18.4 18.3 25.7 27.8 26.0 28.6 24.8 23.9
Std Deviation (1e-4) 19.5 6.9 4.4 3.7 3.7 3.1 2.8 2.6 2.8 2.8 2.7 2.6 4.8 4.9 4.8 7.1 7.5 7.7 7.3 6.8 5.6
Intensity Change (Log2) – – – -0.11 -0.24 -0.34 -0.52 -0.53 -0.5 -0.59 -0.57 -0.58 0.28 0.15 0.14 0.63 0.74 0.65 0.78 0.58 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.1 2.1 4.4 1.8 2.8 3.4 2.7 4.4 2.5 -0.3 0 2.2 0 0 0
Bud 0 0 0 0 3.8 4.0 0 0 3.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 0 -0.1 -1.0 0.7 1.1 -0.1 0.4 2.8 0.7 4.1 6.6 0 0 0
Cytoplasm 3.6 4.2 4.1 3.6 3.1 4.3 3.5 4.6 5.1 -0.1 3.1 1.8 0 0 0
Endoplasmic Reticulum -4.1 -6.2 -7.8 -6.4 -7.1 -8.2 -8.7 -7.4 -9.6 0.8 -2.1 -3.8 -11.1 -8.5 -9.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.9 0 0 0 0 0 0 0 0 1.5 0 0 0 0 0
Mitochondria 0 0 0 3.7 4.6 4.1 4.1 5.5 6.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.7 -2.6 -1.6 0.2 0.1 -2.0 0.3 0.4 -0.4 -2.3 -3.8 -3.1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 3.8 2.1 1.5 1.0 1.4 3.9 -1.7 0.8 -1.3 -1.4 -0.3 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available