Standard name | |
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Human Ortholog | |
Description | Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 8 | 11 | 20 | 23 | 43 | 23 | 31 | 28 | 41 | 33 | 24 | 8 | 7 | 20 | 1 | 2 | 2 | 3 | 8 | 2 | 2 | 1 | 1 |
Bud | 0 | 0 | 0 | 2 | 3 | 6 | 8 | 16 | 13 | 10 | 2 | 13 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 10 | 10 | 6 | 10 | 10 | 7 | 15 | 13 | 15 | 9 | 25 | 11 | 25 | 54 | 5 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 52 | 26 | 50 | 63 | 64 | 62 | 58 | 55 | 83 | 53 | 69 | 21 | 34 | 34 | 14 | 8 | 8 | 1 | 6 | 1 | 2 | 3 | 3 |
Endoplasmic Reticulum | 30 | 463 | 289 | 210 | 218 | 204 | 241 | 235 | 156 | 297 | 148 | 158 | 244 | 165 | 205 | 2 | 0 | 0 | 73 | 630 | 352 | 191 | 173 | 228 |
Endosome | 0 | 0 | 3 | 2 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 2 |
Golgi | 0 | 13 | 4 | 21 | 5 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 8 | 3 | 6 | 7 | 3 | 4 | 0 | 3 | 1 | 1 | 2 | 4 |
Mitochondria | 0 | 0 | 0 | 1 | 2 | 6 | 15 | 23 | 14 | 25 | 22 | 35 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 4 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 6 | 32 | 17 | 15 | 22 | 37 | 37 | 15 | 53 | 27 | 27 | 13 | 3 | 9 | 0 | 0 | 0 | 0 | 10 | 10 | 4 | 4 | 4 |
Nucleolus | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 4 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 120 | 36 | 36 | 73 | 57 | 54 | 50 | 41 | 110 | 17 | 40 | 21 | 15 | 28 | 9 | 3 | 5 | 2 | 17 | 12 | 9 | 13 | 31 |
Unique Cell Count | 51 | 601 | 343 | 296 | 344 | 356 | 382 | 389 | 287 | 532 | 260 | 321 | 282 | 214 | 286 | 40 | 17 | 22 | 91 | 688 | 388 | 215 | 205 | 282 |
Labelled Cell Count | 57 | 675 | 412 | 365 | 415 | 418 | 451 | 472 | 346 | 640 | 315 | 398 | 326 | 254 | 358 | 43 | 18 | 23 | 91 | 688 | 388 | 215 | 205 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 34.6 | 26.6 | 16.6 | 15.3 | 14.0 | 13.1 | 11.6 | 11.5 | 11.8 | 11.0 | 11.2 | 11.1 | 20.1 | 18.4 | 18.3 | 25.7 | 27.8 | 26.0 | 28.6 | 24.8 | 23.9 |
Std Deviation (1e-4) | 19.5 | 6.9 | 4.4 | 3.7 | 3.7 | 3.1 | 2.8 | 2.6 | 2.8 | 2.8 | 2.7 | 2.6 | 4.8 | 4.9 | 4.8 | 7.1 | 7.5 | 7.7 | 7.3 | 6.8 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.24 | -0.34 | -0.52 | -0.53 | -0.5 | -0.59 | -0.57 | -0.58 | 0.28 | 0.15 | 0.14 | 0.63 | 0.74 | 0.65 | 0.78 | 0.58 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.1 | 2.1 | 4.4 | 1.8 | 2.8 | 3.4 | 2.7 | 4.4 | 2.5 | -0.3 | 0 | 2.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3.8 | 4.0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | 0 | -0.1 | -1.0 | 0.7 | 1.1 | -0.1 | 0.4 | 2.8 | 0.7 | 4.1 | 6.6 | 0 | 0 | 0 |
Cytoplasm | 3.6 | 4.2 | 4.1 | 3.6 | 3.1 | 4.3 | 3.5 | 4.6 | 5.1 | -0.1 | 3.1 | 1.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | -4.1 | -6.2 | -7.8 | -6.4 | -7.1 | -8.2 | -8.7 | -7.4 | -9.6 | 0.8 | -2.1 | -3.8 | -11.1 | -8.5 | -9.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.7 | 4.6 | 4.1 | 4.1 | 5.5 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.7 | -2.6 | -1.6 | 0.2 | 0.1 | -2.0 | 0.3 | 0.4 | -0.4 | -2.3 | -3.8 | -3.1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.7 | 3.8 | 2.1 | 1.5 | 1.0 | 1.4 | 3.9 | -1.7 | 0.8 | -1.3 | -1.4 | -0.3 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.395 | 25.3188 | 23.3264 | 23.5232 | 21.3407 | 23.8351 | 25.2477 | 32.367 | 26.7378 | 23.0871 | 25.2464 | 31.0968 | 23.4826 | 25.1357 | 24.756 | 23.2964 | 24.3227 | 25.9997 |
Actin | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0183 | 0.0002 | 0.0166 | 0.0194 | 0.0132 | 0.0004 | 0.0059 | 0.0001 | 0.0013 | 0.0169 | 0.0165 | 0.001 |
Bud | 0 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0005 | 0.0038 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0012 | 0.0004 | 0 |
Bud Neck | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0011 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0024 | 0.0006 | 0.0004 |
Bud Periphery | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0036 | 0.0004 | 0.0014 | 0.0202 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0018 | 0.0015 | 0.0001 |
Bud Site | 0.0003 | 0.002 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0043 | 0.0094 | 0.0005 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0093 | 0.0005 | 0.0001 |
Cell Periphery | 0.001 | 0.0182 | 0.0024 | 0.0004 | 0.005 | 0.0014 | 0.002 | 0.0027 | 0.0043 | 0.0064 | 0.0013 | 0.0006 | 0.0007 | 0.0062 | 0.0071 | 0.0063 | 0.001 | 0.0007 |
Cytoplasm | 0.0109 | 0.0421 | 0.0279 | 0.0245 | 0.0273 | 0.0062 | 0.008 | 0.013 | 0.0134 | 0.0302 | 0.0024 | 0.0063 | 0.0037 | 0.0307 | 0.002 | 0.016 | 0.0034 | 0.0058 |
Cytoplasmic Foci | 0.0018 | 0.0009 | 0.0006 | 0.0001 | 0.0027 | 0.0006 | 0.0076 | 0.0002 | 0.0049 | 0.0023 | 0.0075 | 0.0025 | 0.0188 | 0.0001 | 0.003 | 0.0023 | 0.0005 | 0.0026 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.7786 | 0.7924 | 0.9007 | 0.9263 | 0.794 | 0.8384 | 0.7437 | 0.9277 | 0.8309 | 0.8328 | 0.7317 | 0.8199 | 0.7732 | 0.8804 | 0.9176 | 0.8246 | 0.7173 | 0.8287 |
Endosome | 0.0043 | 0.0025 | 0.002 | 0.0004 | 0.0085 | 0.001 | 0.0109 | 0.0009 | 0.0133 | 0.0159 | 0.0332 | 0.0074 | 0.0063 | 0.0005 | 0.0049 | 0.0082 | 0.0094 | 0.0065 |
Golgi | 0.0006 | 0.0003 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0039 | 0.0001 | 0.0063 | 0.0052 | 0.0232 | 0.0022 | 0.0033 | 0.0001 | 0.002 | 0.0027 | 0.0039 | 0.0008 |
Lipid Particles | 0.0046 | 0.0132 | 0.0019 | 0.0004 | 0.0062 | 0.0013 | 0.0101 | 0.0014 | 0.0073 | 0.0029 | 0.0401 | 0.0003 | 0.0151 | 0.0044 | 0.0031 | 0.0027 | 0.0029 | 0.0008 |
Mitochondria | 0.0006 | 0.0002 | 0.0011 | 0 | 0.0017 | 0.0001 | 0.0024 | 0.0001 | 0.0022 | 0.0014 | 0.0049 | 0.002 | 0.014 | 0 | 0.0039 | 0.0006 | 0.0289 | 0.0015 |
None | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0039 | 0.0003 | 0.0116 | 0.0001 | 0.011 | 0.0012 | 0.0006 | 0.0023 | 0.0049 | 0 | 0.0002 | 0.0004 | 0.0007 | 0.0005 |
Nuclear Periphery | 0.1565 | 0.0757 | 0.0444 | 0.0411 | 0.0924 | 0.1276 | 0.1486 | 0.0331 | 0.0553 | 0.0381 | 0.115 | 0.1443 | 0.1276 | 0.0608 | 0.0322 | 0.0786 | 0.1771 | 0.1259 |
Nucleolus | 0.0006 | 0.0007 | 0.0001 | 0 | 0.0056 | 0.0009 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0001 |
Nucleus | 0.0168 | 0.0181 | 0.0049 | 0.0031 | 0.0145 | 0.0089 | 0.008 | 0.0011 | 0.003 | 0.0011 | 0.0032 | 0.0017 | 0.0075 | 0.0025 | 0.001 | 0.0033 | 0.0111 | 0.0072 |
Peroxisomes | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0021 | 0 | 0.0007 | 0.0003 | 0.0019 | 0.0005 | 0.0068 | 0 | 0.0028 | 0.0002 | 0.0006 | 0.0003 |
Punctate Nuclear | 0.0031 | 0.0004 | 0.0007 | 0.0001 | 0.0081 | 0.0006 | 0.0033 | 0.0001 | 0.0008 | 0.0004 | 0.0013 | 0.0001 | 0.0021 | 0.0001 | 0.0077 | 0.0005 | 0.0009 | 0.0009 |
Vacuole | 0.0157 | 0.0296 | 0.0096 | 0.0028 | 0.0216 | 0.0095 | 0.0095 | 0.017 | 0.0162 | 0.004 | 0.014 | 0.005 | 0.0048 | 0.0122 | 0.0096 | 0.017 | 0.0105 | 0.0126 |
Vacuole Periphery | 0.0035 | 0.0012 | 0.0014 | 0.0003 | 0.0046 | 0.0018 | 0.0037 | 0.0018 | 0.0064 | 0.0044 | 0.0053 | 0.0035 | 0.0032 | 0.0011 | 0.0012 | 0.0047 | 0.0112 | 0.0033 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 114.7162 | 65.547 | 54.9146 | 105.9843 | 109.6343 | 128.8366 | 80.438 | 84.7721 | 99.422 | 117.0106 |
Translational Efficiency | 1.1723 | 1.3127 | 1.2236 | 1.0257 | 1.0972 | 1.1973 | 1.1255 | 0.9151 | 0.9912 | 0.9191 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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