Standard name
Description Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.06 0.1 0.06 0.06 0.08 0 0 – 0.05 0.05 0.09 0.1 0.06 0.05 0.08 0.14 0.09 0.05 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.07 0 0.06 0.08 – 0.06 0.08 0.06 0 0.08 0.14 0.17 0.19 0.23 0 0 0 0 0 0
Cytoplasm 0.14 0.14 0.17 0.23 0.33 0.26 0.33 0.36 – 0.29 0.28 0.23 0.11 0.11 0.11 0.44 0.52 0.58 0 0 0 0 0 0
Endoplasmic Reticulum 0.82 0.88 0.79 0.61 0.25 0.53 0.35 0.2 – 0.25 0.21 0.39 0.85 0.85 0.84 0 0 0 0.86 0.9 0.89 0.81 0.84 0.81
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0.07 0 0 0.08 0.05 0 0 0 – 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.13 0.2 0.2 – 0.28 0.43 0.4 0 0 0 0.13 0.09 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.14 0.35 0.11 0.17 0.25 – 0.18 0.12 0.08 0 0 0 0.29 0.1 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 8 23 9 15 22 13 21 – 19 17 36 35 25 23 9 11 7 15 4 6 15 10 13
Bud 3 0 2 0 9 8 10 15 – 16 21 14 8 1 0 2 2 1 2 0 0 2 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 1 0 0 – 2 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 7 4 18 12 19 38 – 23 29 27 8 32 66 18 15 18 1 0 3 1 3 4
Cytoplasm 44 20 42 35 81 74 113 173 – 107 97 95 38 45 53 48 41 45 6 2 7 10 13 11
Endoplasmic Reticulum 264 122 191 93 61 154 119 93 – 92 74 165 289 349 396 1 3 0 283 128 221 309 277 289
Endosome 2 0 5 1 4 5 11 16 – 15 2 3 0 1 2 5 0 0 3 1 0 3 1 4
Golgi 22 2 10 12 12 0 2 3 – 0 0 2 7 10 7 6 3 4 6 2 3 10 6 4
Mitochondria 3 1 5 5 18 39 68 96 – 105 149 167 0 0 0 14 7 1 3 0 2 7 6 7
Nucleus 1 0 0 0 1 1 5 4 – 2 3 1 1 1 1 1 2 0 0 0 0 0 0 1
Nuclear Periphery 0 3 4 2 2 3 4 3 – 3 2 2 0 1 0 0 0 0 2 0 0 2 1 1
Nucleolus 0 0 0 0 0 0 1 1 – 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 – 3 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 11 4 9 21 88 32 59 117 – 68 43 33 9 14 9 31 8 6 3 2 0 11 4 6
Unique Cell Count 321 139 241 152 248 290 338 476 370 347 420 342 409 473 108 79 78 331 142 250 383 330 356
Labelled Cell Count 368 163 298 182 309 351 425 581 456 439 548 395 479 557 137 93 82 331 142 250 383 330 356


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.6 18.2 13.3 12.3 12.5 9.9 9.1 9.8 – 9.0 8.5 8.8 15.1 15.4 14.8 10.3 10.3 10.7 16.0 17.1 16.5
Std Deviation (1e-4) 4.1 3.2 2.8 2.4 5.8 2.7 2.5 2.7 – 2.4 2.1 2.2 2.9 2.9 2.6 2.0 2.0 2.1 3.4 3.5 3.5
Intensity Change (Log2) – – – -0.1 -0.09 -0.42 -0.54 -0.44 – -0.57 -0.64 -0.6 0.18 0.22 0.16 -0.37 -0.37 -0.3 0.27 0.37 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.3 -1.4 -0.8 -2.8 -2.7 – -2.1 -2.2 -0.4 0.3 -1.6 -2.4 -0.4 1.1 -0.1
Bud 0 2.1 0 0 1.9 – 2.5 3.2 2.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 0.8 1.6 2.6 – 1.9 2.7 2.0 -0.4 2.6 4.6 4.6 4.9 5.8
Cytoplasm 1.4 3.9 2.2 4.3 5.2 – 3.2 3.0 1.6 -2.2 -2.3 -2.3 5.3 6.1 6.9
Endoplasmic Reticulum -3.9 -12.1 -6.3 -10.5 -15.4 – -13.2 -13.9 -9.9 1.6 2.0 1.5 -13.6 -11.9 -12.4
Endosome 0 0 0 0.9 1.0 – 1.3 0 0 0 0 0 0 0 0
Golgi 1.6 0.4 0 0 0 – 0 0 0 -1.5 -1.2 -2.2 0 0 0
Mitochondria 0 2.7 4.7 6.4 6.6 – 8.3 11.1 10.6 0 0 0 4.1 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 3.7 8.8 3.1 5.1 6.9 – 5.3 3.6 2.1 -0.8 -0.2 -1.5 6.8 0 0