Standard name
Human Ortholog
Description Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.55 0.63 0.73 0.77 0.58 0.59 0.64 0.61 0.42 0.34 0.3 0.87 0.96 1.0 0.69 0.58 0.32 0.37 0.37 0.57 0.83 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.1 0.2 0.23 0.18 0.51 0.56 0.49 0.58 0.74 0.82 0.8 0 0 0 0 0 0.06 0.06 0.09 0.06 0.06 0
Nucleus 0.32 0.24 0.17 0.06 0 0.06 0 0.06 0 0 0.07 0.05 0.05 0 0 0.17 0.24 0.33 0.28 0.2 0.13 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0
Vac/Vac Membrane 0.11 0.2 0.06 0.05 0.08 0.08 0 0 0 0 0 0.05 0.08 0 0 0.13 0.17 0.18 0.13 0.18 0.13 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 3 1
Bud 2 1 0 0 1 1 2 1 5 0 2 4 0 0 0 0 0 3 9 2 1 4 6
Bud Neck 0 1 1 0 0 0 0 2 1 0 0 1 0 0 0 2 1 1 2 0 0 0 3
Bud Site 0 0 0 0 0 0 2 1 2 1 2 1 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 1 1
Cytoplasm 80 169 98 91 110 128 121 133 113 112 54 56 192 298 223 202 151 69 129 59 94 217 199
Endoplasmic Reticulum 0 1 3 0 0 1 0 0 0 2 1 1 1 1 0 21 13 0 0 1 1 0 2
Endosome 0 0 0 2 4 0 0 0 0 1 0 0 2 2 0 7 7 1 3 2 1 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Mitochondria 97 32 31 29 26 112 116 101 107 199 128 151 5 1 0 4 6 12 19 14 9 14 9
Nucleus 63 75 27 8 3 14 8 12 7 12 11 9 11 1 0 50 62 72 98 32 21 1 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 2 0 1 1 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 1 0 2 1 0 0 0 0 1 0 0 0 1 1 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 4 2 2 1 1 2 1 5 1 0 0 0 3 7 24 11 7 1 4
Vac/Vac Membrane 21 63 9 6 11 17 6 6 5 10 3 9 18 12 0 39 43 39 44 28 21 7 9
Unique Cell Count 199 309 156 124 143 219 206 207 185 268 157 188 221 309 223 291 260 218 348 160 168 263 252
Labelled Cell Count 263 343 169 138 159 276 258 259 242 341 202 238 232 316 224 326 287 218 348 160 168 263 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.1 3.5 3.5 3.7 2.8 2.6 3.0 2.7 2.4 2.5 2.5 4.3 4.0 4.2 4.7 5.9 5.7 4.1 4.1 4.9
Std Deviation (1e-4) 0.6 0.8 0.8 1.3 2.2 0.6 1.2 1.3 1.2 0.7 0.9 1.0 1.2 1.4 1.9 1.4 1.7 1.6 1.6 1.8 2.1
Intensity Change (Log2) -0.01 0.06 -0.35 -0.43 -0.25 -0.38 -0.55 -0.47 -0.53 0.27 0.19 0.24 0.4 0.74 0.69 0.22 0.22 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 2.5 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0646 0.0022 0.0006 0.0288 0.0002 0.0003 0.0397 0.0005 0.0057 0.0007 0.0433 0.0074 0.014 0.0001 0.0002 0.0003 0.0001 0.0009
Bud 0.0008 0.0101 0.0004 0.0003 0 0.002 0.0016 0.0068 0.0036 0.0023 0.0006 0.0024 0.001 0.0002 0.0014 0.0001 0.0094 0.0057
Bud Neck 0.0149 0.0004 0.0005 0.0002 0.0001 0.0042 0.0018 0.0011 0.0006 0.0003 0.001 0.0028 0.0003 0.0003 0.0001 0.0024 0.0005 0.0045
Bud Periphery 0.0009 0.0094 0.0006 0.0004 0 0.0035 0.0027 0.0063 0.0035 0.0065 0.0009 0.0085 0.001 0.0001 0.0019 0 0.0101 0.019
Bud Site 0.0277 0.0112 0.0033 0.0024 0.0001 0.0012 0.0102 0.0127 0.0084 0.0007 0.0016 0.0004 0.0009 0.0013 0.0011 0 0.0007 0.0018
Cell Periphery 0.0005 0.0009 0.0003 0.0001 0.0001 0.0004 0.0006 0.0007 0.0004 0.0001 0.0003 0.0001 0.0002 0.0004 0.0003 0.0001 0.0002 0.0009
Cytoplasm 0.0225 0.0722 0.0519 0.0337 0.0803 0.0733 0.0634 0.0643 0.0676 0.0414 0.0785 0.037 0.0249 0.0838 0.1254 0.2229 0.0418 0.0736
Cytoplasmic Foci 0.0586 0.0018 0.0027 0.0075 0.004 0.0018 0.0221 0.0021 0.0037 0.0031 0.0459 0.0019 0.0219 0.0008 0.0019 0.001 0.0009 0.0035
Eisosomes 0.0009 0.0003 0.0005 0.0005 0.0001 0.0001 0.0006 0.0003 0.0003 0.0001 0.0003 0.0001 0.0001 0.0013 0.0006 0.0004 0.0002 0.0002
Endoplasmic Reticulum 0.0011 0.0039 0.0019 0.0008 0.003 0.0023 0.0063 0.0021 0.001 0.0006 0.0019 0.001 0.0421 0.0007 0.0037 0.0016 0.0006 0.0012
Endosome 0.0174 0.0011 0.001 0.0018 0.0046 0.0019 0.0253 0.001 0.0006 0.0009 0.048 0.0017 0.6323 0.0001 0.0009 0.0006 0.0001 0.0018
Golgi 0.0135 0.0001 0.0002 0.0037 0.0001 0.0001 0.0149 0.0001 0.0004 0.0001 0.0131 0.0011 0.0585 0 0.0001 0 0 0.0001
Lipid Particles 0.0431 0.0003 0.0005 0.0046 0.001 0.0002 0.0586 0.0003 0.0005 0.0001 0.0227 0.0035 0.0108 0.0001 0.0003 0 0 0.0002
Mitochondria 0.0066 0.0028 0.0264 0.0154 0.0004 0.0012 0.036 0.0015 0.0113 0.0029 0.011 0.0014 0.0319 0.0006 0.0007 0.0001 0.0008 0.0012
None 0.1939 0.5802 0.7397 0.8725 0.8754 0.2608 0.2374 0.5994 0.7684 0.875 0.6358 0.2548 0.0096 0.7408 0.8323 0.7473 0.7317 0.3853
Nuclear Periphery 0.0058 0.0053 0.0024 0.0002 0.0046 0.0043 0.0188 0.0081 0.0024 0.0031 0.0011 0.0049 0.0952 0.0011 0.0044 0.0023 0.0031 0.0045
Nucleolus 0.0126 0.0032 0.0043 0.0001 0.0009 0.0128 0.0128 0.0085 0.0024 0.0022 0.0031 0.015 0.0004 0.0007 0.0009 0.0001 0.0022 0.0076
Nucleus 0.4486 0.284 0.1561 0.0007 0.0191 0.6163 0.3495 0.2637 0.1006 0.0426 0.0738 0.635 0.0074 0.1664 0.0169 0.0188 0.1939 0.474
Peroxisomes 0.0333 0.0002 0.0002 0.0257 0.0001 0.0001 0.0604 0.0002 0.0046 0.0008 0.0082 0.0075 0.0017 0 0.0001 0 0 0.0003
Punctate Nuclear 0.0295 0.0023 0.005 0.0004 0.0036 0.0086 0.029 0.0142 0.0125 0.0156 0.004 0.011 0.0032 0.0003 0.0017 0.0012 0.0006 0.0088
Vacuole 0.0025 0.0068 0.0014 0.0002 0.0021 0.0042 0.0062 0.0054 0.0012 0.0007 0.0034 0.002 0.015 0.0007 0.0043 0.0005 0.0023 0.0044
Vacuole Periphery 0.0007 0.0012 0.0002 0.0001 0.0002 0.0006 0.0018 0.0007 0.0002 0.0003 0.0014 0.0005 0.0277 0.0001 0.0007 0.0001 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3757 13.4331 42.2452 65.4446 12.5248 18.5944 18.1096 23.5725 41.1295 25.565
Translational Efficiency 0.5371 0.8821 0.7373 0.7065 0.8342 0.5687 0.5214 0.5109 0.931 0.5626

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
670 233 83 1400 1669 661 1320 1062 2339 894 1403 2462

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 654.59 701.04 878.08 872.89 681.63 683.15 880.65 880.19 673.88 687.81 880.50 876.04
Standard Deviation 70.47 106.15 93.40 132.80 76.74 113.14 107.33 136.61 75.99 111.64 106.56 134.51
Intensity Change Log 2 0.098905 0.423761 0.415208 0.003214 0.369580 0.368826 0.050884 0.396376 0.391734

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000039 0.000438 0.000559 0.000918 0.000128 0.001354 0.000332 0.001505 0.000102 0.001116 0.000345 0.001171
Bud Neck 0.003582 0.037451 0.001509 0.017941 0.007790 0.030347 0.005298 0.015812 0.006585 0.032199 0.005074 0.017022
Bud Site 0.001144 0.012797 0.001030 0.013884 0.001084 0.017872 0.004391 0.025187 0.001101 0.016549 0.004192 0.018759
Cell Periphery 0.000189 0.000185 0.000237 0.000183 0.000167 0.000346 0.000120 0.000586 0.000174 0.000304 0.000127 0.000357
Cytoplasm 0.371487* 0.244181* 0.102134* 0.109454* 0.343170* 0.262307* 0.118914* 0.126586* 0.351281* 0.257583* 0.117921* 0.116844*
Cytoplasmic Foci 0.084759 0.147881 0.024604 0.005751 0.104593 0.135027 0.003095 0.010695 0.098912 0.138377 0.004368 0.007884
Eisosomes 0.000085 0.000105 0.000121 0.000030 0.000096 0.000129 0.000029 0.000040 0.000093 0.000123 0.000035 0.000034
Endoplasmic Reticulum 0.000424 0.002058 0.006966 0.006362 0.000595 0.002391 0.004288 0.007056 0.000546 0.002304 0.004446 0.006661
Endosome 0.003311 0.023614 0.003640 0.006888 0.003490 0.013706 0.001189 0.010529 0.003438 0.016288 0.001334 0.008459
Golgi 0.000332 0.004932 0.000117 0.003038 0.000510 0.005199 0.000139 0.007245 0.000459 0.005129 0.000138 0.004852
Lipid Particles 0.006308 0.005343 0.005366 0.001071 0.007798 0.008198 0.001775 0.001202 0.007372 0.007454 0.001987 0.001128
Mitochondria 0.001445 0.010038 0.000491 0.004532 0.002352 0.014632 0.001143 0.004854 0.002092 0.013434 0.001104 0.004671
Mitotic Spindle 0.001925 0.007735 0.000062 0.044957 0.000674 0.022742 0.006833 0.042358 0.001032 0.018831 0.006432 0.043836
None 0.014427 0.007763 0.003181 0.007072 0.007773 0.016418 0.006466 0.010339 0.009679 0.014162 0.006271 0.008481
Nuclear Periphery 0.000759 0.001064 0.001281 0.003832 0.001044 0.001287 0.001543 0.002692 0.000962 0.001229 0.001528 0.003340
Nuclear Periphery Foci 0.000288 0.000674 0.001619 0.001959 0.000284 0.000737 0.000512 0.001808 0.000285 0.000721 0.000578 0.001894
Nucleolus 0.003157 0.005162 0.002935 0.001478 0.003308 0.006300 0.001204 0.002034 0.003265 0.006004 0.001306 0.001718
Nucleus 0.490490* 0.425580* 0.794803* 0.727917* 0.496284* 0.396107* 0.817756* 0.693567* 0.494625* 0.403788* 0.816398* 0.713100*
Peroxisomes 0.001218 0.016605 0.000684 0.001678 0.003840 0.019529 0.000129 0.001057 0.003089 0.018767 0.000162 0.001410
Vacuole 0.014200 0.044901 0.048424 0.033675 0.014626 0.042976 0.024523 0.031332 0.014504 0.043478 0.025936 0.032664
Vacuole Periphery 0.000430 0.001493 0.000238 0.007381 0.000395 0.002395 0.000322 0.003515 0.000405 0.002160 0.000317 0.005713

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.63 -5.14 -8.31 -3.21 -1.23 -3.88 -3.61 -7.16 0.46 -6.01 -4.33 -5.61 -9.75 0.40 -7.36
Bud Neck -4.44 1.89 -10.48 2.47 -13.01 -7.12 2.20 -6.36 3.93 -8.13 -8.34 1.33 -10.71 4.56 -11.75
Bud Site -3.77 -0.61 -6.52 1.00 -6.57 -6.53 -4.19 -6.42 0.44 -5.12 -7.46 -4.13 -9.12 1.74 -6.40
Cell Periphery 0.14 -1.41 1.51 0.73 2.90 -2.83 4.07 -1.71 0.27 -2.37 -2.68 4.27 -1.25 1.33 -2.71
Cytoplasm 6.35 12.78 23.98 8.00 0.62 6.67 27.77 27.81 13.90 1.51 9.07 32.23 38.49 16.93 2.77
Cytoplasmic Foci -4.36 7.96 15.91 11.03 3.49 -3.73 24.51 23.27 18.36 -2.57 -5.54 28.43 28.39 21.63 -0.04
Eisosomes -1.90 -2.86 5.38 5.55 5.47 -2.10 7.68 5.44 5.14 -4.98 -2.57 8.19 7.50 6.66 -2.13
Endoplasmic Reticulum -4.88 -5.40 -19.52 -11.91 -0.01 -3.62 -17.45 -15.53 -9.06 -5.33 -4.75 -18.48 -24.65 -13.09 -6.84
Endosome -4.63 -0.79 0.17 4.65 0.89 -6.51 6.16 -1.87 4.81 -4.43 -7.87 6.55 -1.14 7.28 -5.23
Golgi -2.50 1.98 -1.97 2.04 -2.61 -3.61 5.49 -2.64 1.48 -3.12 -4.35 5.68 -3.15 2.82 -4.00
Lipid Particles 0.73 0.74 5.30 6.79 4.57 -0.31 7.49 9.84 7.52 0.48 -0.09 7.64 11.28 8.97 1.04
Mitochondria -2.84 1.94 -2.95 2.24 -7.58 -5.33 4.24 -1.59 4.96 -5.05 -6.03 3.94 -3.02 5.44 -7.53
Mitotic Spindle -1.77 1.16 -8.14 -5.36 -9.27 -5.25 -4.10 -8.23 -2.00 -6.49 -5.50 -3.72 -12.00 -3.41 -9.19
None 2.42 5.55 3.70 0.57 -4.20 -2.77 2.89 0.53 2.92 -1.78 -1.83 5.03 2.92 3.12 -2.45
Nuclear Periphery -1.80 -4.61 -11.67 -9.35 -8.98 -1.11 -11.85 -9.62 -7.49 -5.16 -1.69 -13.73 -14.65 -11.42 -8.64
Nuclear Periphery Foci -1.78 -3.16 -5.21 -2.08 1.00 -3.38 -6.11 -4.31 -2.99 -2.90 -3.79 -6.91 -6.66 -3.93 -3.57
Nucleolus -2.12 0.55 1.76 3.06 0.69 -2.68 5.88 2.96 3.99 -3.45 -3.33 6.37 3.54 4.92 -4.04
Nucleus 2.16 -8.95 -16.90 -12.06 1.03 6.43 -28.32 -16.71 -18.59 7.09 6.60 -31.32 -25.55 -24.03 7.30
Peroxisomes -4.72 2.64 1.38 4.87 -0.14 -6.54 5.28 4.58 8.30 -3.95 -7.98 5.80 4.52 9.54 -2.63
Vacuole -5.54 -4.14 -13.14 -3.17 0.08 -7.93 -10.65 -11.99 -5.40 -3.52 -9.64 -11.40 -17.86 -6.49 -3.61
Vacuole Periphery -2.87 1.36 -2.48 -1.23 -2.72 -3.53 2.16 -1.87 1.09 -2.12 -4.08 2.24 -3.09 0.01 -3.37