Standard name | |
---|---|
Human Ortholog | |
Description | Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 77 | 127 | 79 | 73 | 20 | 53 | 39 | 26 | 40 | – | 20 | 3 | 50 | 111 | 122 | 62 | 64 | 73 | 110 | 159 | 156 | 69 | 111 | 139 |
Bud | 10 | 14 | 33 | 29 | 42 | 31 | 50 | 68 | 61 | – | 53 | 31 | 9 | 24 | 31 | 3 | 4 | 5 | 0 | 2 | 0 | 0 | 2 | 1 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 16 | 5 | 10 | 7 | 9 |
Bud Site | 1 | 3 | 1 | 1 | 1 | 5 | 4 | 4 | 7 | – | 6 | 5 | 0 | 1 | 1 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 16 | 32 | 26 | 26 | 14 | 12 | 14 | 15 | 18 | – | 17 | 21 | 13 | 30 | 70 | 16 | 22 | 20 | 11 | 14 | 20 | 12 | 20 | 38 |
Cytoplasm | 32 | 63 | 37 | 43 | 38 | 52 | 77 | 115 | 49 | – | 34 | 60 | 25 | 53 | 60 | 37 | 27 | 33 | 2 | 6 | 1 | 3 | 8 | 6 |
Endoplasmic Reticulum | 91 | 201 | 77 | 41 | 50 | 62 | 63 | 40 | 61 | – | 25 | 17 | 53 | 91 | 73 | 8 | 8 | 13 | 134 | 152 | 73 | 44 | 76 | 68 |
Endosome | 12 | 2 | 12 | 6 | 20 | 5 | 21 | 23 | 3 | – | 0 | 10 | 1 | 4 | 0 | 11 | 9 | 16 | 4 | 11 | 10 | 5 | 6 | 9 |
Golgi | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 24 | 46 | 40 | 9 | 16 | 18 | 5 | 6 | 9 |
Mitochondria | 23 | 62 | 82 | 139 | 109 | 164 | 259 | 352 | 367 | – | 271 | 321 | 14 | 45 | 67 | 14 | 26 | 15 | 25 | 38 | 37 | 21 | 37 | 35 |
Nucleus | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 1 | – | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 15 | 5 | 29 | 29 | 96 | 20 | 43 | 58 | 36 | – | 18 | 18 | 4 | 12 | 10 | 18 | 15 | 35 | 4 | 3 | 16 | 2 | 7 | 7 |
SpindlePole | 2 | 2 | 8 | 2 | 11 | 3 | 10 | 27 | 10 | – | 9 | 7 | 0 | 4 | 3 | 15 | 23 | 22 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 7 | 7 | 0 | 2 | 1 | 4 | 10 | 2 | – | 5 | 11 | 0 | 0 | 1 | 5 | 5 | 11 | 3 | 15 | 8 | 6 | 13 | 8 |
Unique Cell Count | 224 | 377 | 271 | 254 | 289 | 286 | 429 | 569 | 480 | 332 | 394 | 126 | 279 | 318 | 165 | 168 | 191 | 313 | 443 | 352 | 184 | 304 | 338 | |
Labelled Cell Count | 296 | 521 | 394 | 391 | 404 | 409 | 584 | 743 | 655 | 460 | 508 | 172 | 376 | 438 | 213 | 251 | 286 | 313 | 443 | 352 | 184 | 304 | 338 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 6.0 | 5.4 | 5.1 | 5.1 | 4.8 | 4.6 | 4.4 | 4.0 | – | 3.9 | 3.7 | 5.5 | 5.7 | 5.4 | 8.6 | 9.3 | 9.9 | 6.3 | 6.3 | 6.6 |
Std Deviation (1e-4) | 1.4 | 1.0 | 0.8 | 1.3 | 1.6 | 1.4 | 1.5 | 1.4 | 1.1 | – | 1.1 | 0.8 | 1.0 | 0.9 | 0.9 | 1.7 | 2.1 | 2.3 | 1.2 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.07 | -0.16 | -0.22 | -0.29 | -0.41 | – | -0.45 | -0.54 | 0.05 | 0.08 | 0.02 | 0.69 | 0.8 | 0.88 | 0.24 | 0.24 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | -6.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.1 | 2.6 | 2.4 | 1.8 | 1.9 | 2.0 |
Bud | -0.3 | 0.8 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.5 | -1.4 | -0.9 | -3.8 | -3.6 | -3.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | -2.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.2 | 0.4 | 4.1 | 0 | 1.1 | 0.3 |
Cytoplasm | 1.0 | -0.2 | 1.5 | 1.5 | 2.3 | -1.4 | – | -1.3 | 0.6 | 1.6 | 1.7 | 1.7 | 2.4 | 0.7 | 1.1 |
Endoplasmic Reticulum | -3.4 | -3.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.7 | 1.1 | -1.5 | -6.0 | -6.1 | -5.8 |
Endosome | -1.3 | 1.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.1 | -3.8 | 1.0 | 0.4 | 1.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.9 | 8.7 | 7.4 |
Mitochondria | 5.7 | 1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -4.1 | -3.9 | -2.6 | -5.3 | -3.5 | -5.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.3 | 6.4 | 0 | 0 | 0 | 0 | – | 0 | 0 | -2.5 | -2.9 | -3.7 | 0.1 | -0.6 | 2.3 |
SpindlePole | 0 | 0.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.2 | -1.8 | 2.8 | 4.3 | 3.7 |
Vacuole | 0 | 0 | 0 | 0 | -0.8 | 0 | – | -0.9 | 0.2 | 0 | 0 | 0 | 0 | 0 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.1914 | 4.7543 | 4.9386 | 4.8479 | 3.7366 | 4.5358 | 2.4291 | 3.3579 | 3.502 | 2.8633 | 2.3312 | 2.8331 | 4.2247 | 4.1868 | 4.3522 | 4.2006 | 3.8503 | 3.5147 |
Actin | 0.1755 | 0.1349 | 0.2396 | 0.2338 | 0.166 | 0.1831 | 0.171 | 0.1213 | 0.1459 | 0.22 | 0.305 | 0.1427 | 0.2411 | 0.1918 | 0.2761 | 0.2681 | 0.3451 | 0.2437 |
Bud | 0.0015 | 0.0021 | 0.0043 | 0.0032 | 0.0037 | 0.001 | 0.0212 | 0.0019 | 0.0152 | 0.0026 | 0.0006 | 0.0012 | 0.0008 | 0.0028 | 0.0154 | 0.0008 | 0.0029 | 0.0019 |
Bud Neck | 0.0222 | 0.0108 | 0.0333 | 0.0438 | 0.0692 | 0.0983 | 0.0304 | 0.0091 | 0.0162 | 0.0094 | 0.0076 | 0.1034 | 0.0144 | 0.0088 | 0.0189 | 0.0521 | 0.0397 | 0.0693 |
Bud Periphery | 0.006 | 0.004 | 0.0188 | 0.0292 | 0.0394 | 0.0039 | 0.0138 | 0.0018 | 0.006 | 0.0074 | 0.0013 | 0.0039 | 0.0023 | 0.0055 | 0.0186 | 0.0088 | 0.0222 | 0.0076 |
Bud Site | 0.0134 | 0.106 | 0.0379 | 0.003 | 0.0456 | 0.0029 | 0.0547 | 0.1023 | 0.0241 | 0.0138 | 0.0026 | 0.002 | 0.013 | 0.0992 | 0.0693 | 0.0029 | 0.0131 | 0.0064 |
Cell Periphery | 0.0315 | 0.0159 | 0.0286 | 0.0563 | 0.0123 | 0.0179 | 0.0298 | 0.031 | 0.0322 | 0.0236 | 0.0195 | 0.0303 | 0.0303 | 0.0312 | 0.0443 | 0.063 | 0.046 | 0.0207 |
Cytoplasm | 0.1652 | 0.231 | 0.2119 | 0.2407 | 0.151 | 0.2681 | 0.1754 | 0.3491 | 0.2875 | 0.2735 | 0.1332 | 0.2599 | 0.1853 | 0.2062 | 0.1871 | 0.1097 | 0.1292 | 0.3406 |
Cytoplasmic Foci | 0.0764 | 0.0884 | 0.0766 | 0.0279 | 0.0371 | 0.069 | 0.0866 | 0.0645 | 0.0744 | 0.0697 | 0.0989 | 0.0972 | 0.0777 | 0.0692 | 0.06 | 0.047 | 0.0752 | 0.0652 |
Eisosomes | 0.0054 | 0.0017 | 0.0045 | 0.01 | 0.0047 | 0.0033 | 0.0055 | 0.0039 | 0.0037 | 0.0035 | 0.006 | 0.0028 | 0.0073 | 0.0065 | 0.0093 | 0.0242 | 0.0219 | 0.0032 |
Endoplasmic Reticulum | 0.0654 | 0.0865 | 0.0992 | 0.0562 | 0.1124 | 0.1094 | 0.0495 | 0.0487 | 0.0698 | 0.151 | 0.1845 | 0.0945 | 0.0715 | 0.0888 | 0.0338 | 0.0962 | 0.0492 | 0.0612 |
Endosome | 0.07 | 0.0579 | 0.0442 | 0.0377 | 0.0767 | 0.0489 | 0.0345 | 0.0277 | 0.0461 | 0.065 | 0.0287 | 0.0431 | 0.0694 | 0.0483 | 0.0221 | 0.0159 | 0.0287 | 0.0315 |
Golgi | 0.0567 | 0.071 | 0.0596 | 0.0623 | 0.0525 | 0.0616 | 0.0211 | 0.0388 | 0.0682 | 0.0499 | 0.0889 | 0.0626 | 0.0589 | 0.0605 | 0.0441 | 0.0615 | 0.0812 | 0.0366 |
Lipid Particles | 0.1589 | 0.1176 | 0.0696 | 0.0354 | 0.093 | 0.0767 | 0.1639 | 0.0659 | 0.0796 | 0.0738 | 0.0889 | 0.0889 | 0.0888 | 0.0573 | 0.058 | 0.0288 | 0.0752 | 0.0308 |
Mitochondria | 0.015 | 0.0062 | 0.0165 | 0.0465 | 0.0514 | 0.0161 | 0.0076 | 0.0016 | 0.0253 | 0.0045 | 0.0021 | 0.0234 | 0.0055 | 0.0047 | 0.0045 | 0.0038 | 0.0108 | 0.0053 |
None | 0.043 | 0.0405 | 0.0308 | 0.0618 | 0.052 | 0.0237 | 0.0968 | 0.1086 | 0.0787 | 0.0114 | 0.0037 | 0.0247 | 0.097 | 0.1074 | 0.1217 | 0.1955 | 0.0374 | 0.0579 |
Nuclear Periphery | 0.0342 | 0.004 | 0.0045 | 0.0082 | 0.0031 | 0.0022 | 0.0034 | 0.0034 | 0.0091 | 0.0018 | 0.0017 | 0.0015 | 0.0048 | 0.0012 | 0.0013 | 0.0009 | 0.0017 | 0.0015 |
Nucleolus | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0014 | 0.0007 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0008 | 0.0002 | 0.0005 | 0.0003 |
Nucleus | 0.0063 | 0.0012 | 0.0012 | 0.0017 | 0.0007 | 0.0008 | 0.0028 | 0.0014 | 0.0047 | 0.0007 | 0.0003 | 0.0008 | 0.0008 | 0.0005 | 0.0031 | 0.0003 | 0.0006 | 0.001 |
Peroxisomes | 0.031 | 0.0121 | 0.0091 | 0.0017 | 0.0205 | 0.007 | 0.0186 | 0.0035 | 0.0045 | 0.0096 | 0.0236 | 0.0113 | 0.0207 | 0.0062 | 0.006 | 0.0163 | 0.0141 | 0.0087 |
Punctate Nuclear | 0.0127 | 0.0021 | 0.0034 | 0.0007 | 0.0009 | 0.0011 | 0.0062 | 0.0113 | 0.002 | 0.0011 | 0.0011 | 0.0015 | 0.0031 | 0.0009 | 0.0018 | 0.0006 | 0.0024 | 0.0014 |
Vacuole | 0.0055 | 0.0049 | 0.0051 | 0.0219 | 0.0052 | 0.004 | 0.0049 | 0.0032 | 0.005 | 0.0067 | 0.0013 | 0.0031 | 0.0053 | 0.0024 | 0.0035 | 0.0033 | 0.0023 | 0.0047 |
Vacuole Periphery | 0.0032 | 0.0007 | 0.001 | 0.0175 | 0.0023 | 0.0009 | 0.001 | 0.0003 | 0.0012 | 0.0008 | 0.0004 | 0.0008 | 0.0014 | 0.0004 | 0.0004 | 0.0003 | 0.0007 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.4221 | 27.595 | 29.045 | 30.7228 | 25.8305 | 32.8212 | 34.1346 | 32.4543 | 31.86 | 37.4555 |
Translational Efficiency | 0.4441 | 0.3639 | 0.3688 | 0.3567 | 0.2953 | 0.4868 | 0.3752 | 0.3564 | 0.4038 | 0.3427 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
276 | 1441 | 362 | 1500 | 1901 | 2201 | 148 | 827 | 2177 | 3642 | 510 | 2327 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 721.42 | 840.60 | 1289.38 | 1033.86 | 747.85 | 854.47 | 1058.61 | 996.16 | 744.50 | 848.98 | 1222.41 | 1020.46 |
Standard Deviation | 100.55 | 119.62 | 158.29 | 138.12 | 106.46 | 116.87 | 124.15 | 140.38 | 106.09 | 118.16 | 182.28 | 140.09 |
Intensity Change Log 2 | — | 0.220580 | 0.837766 | 0.519130 | — | 0.192281 | 0.501350 | 0.413629 | — | 0.206245 | 0.676327 | 0.466394 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.126886* | 0.106357* | 0.067642 | 0.154216* | 0.055433 | 0.073339 | 0.143464* | 0.225286* | 0.064492 | 0.086403 | 0.089646 | 0.179474* |
Bud Neck | 0.003441 | 0.006568 | 0.004769 | 0.007908 | 0.007209 | 0.010586 | 0.005407 | 0.012553 | 0.006731 | 0.008996 | 0.004954 | 0.009559 |
Bud Site | 0.004693 | 0.013827 | 0.028709 | 0.036773 | 0.006969 | 0.019893 | 0.018263 | 0.043846 | 0.006680 | 0.017493 | 0.025677 | 0.039287 |
Cell Periphery | 0.005833 | 0.021753 | 0.008977 | 0.014826 | 0.011845 | 0.019942 | 0.032142 | 0.049229 | 0.011083 | 0.020658 | 0.015699 | 0.027052 |
Cytoplasm | 0.024645 | 0.092559 | 0.016463 | 0.049040 | 0.137849* | 0.162988* | 0.068001 | 0.040704 | 0.123497* | 0.135122* | 0.031419 | 0.046077 |
Cytoplasmic Foci | 0.060158 | 0.063645 | 0.015813 | 0.056488 | 0.059275 | 0.065128 | 0.055849 | 0.023915 | 0.059387 | 0.064541 | 0.027431 | 0.044912 |
Eisosomes | 0.013485 | 0.039433 | 0.004708 | 0.027504 | 0.056244 | 0.047635 | 0.031667 | 0.051943 | 0.050823 | 0.044390 | 0.012531 | 0.036189 |
Endoplasmic Reticulum | 0.061964 | 0.091479 | 0.556150* | 0.156461* | 0.050428 | 0.082956 | 0.314148* | 0.225455* | 0.051891 | 0.086328 | 0.485922* | 0.180981* |
Endosome | 0.036384 | 0.022462 | 0.037597 | 0.039419 | 0.012670 | 0.017424 | 0.017332 | 0.021082 | 0.015676 | 0.019417 | 0.031717 | 0.032902 |
Golgi | 0.110287* | 0.152833* | 0.025036 | 0.091029 | 0.157675* | 0.131646* | 0.046584 | 0.038494 | 0.151668* | 0.140029* | 0.031289 | 0.072358 |
Lipid Particles | 0.354935* | 0.168623* | 0.166674* | 0.288422* | 0.182214* | 0.165394* | 0.185859* | 0.192637* | 0.204112* | 0.166672* | 0.172241* | 0.254380* |
Mitochondria | 0.013235 | 0.021579 | 0.000154 | 0.003803 | 0.025374 | 0.024466 | 0.000326 | 0.003365 | 0.023835 | 0.023324 | 0.000204 | 0.003647 |
Mitotic Spindle | 0.001197 | 0.000316 | 0.001145 | 0.007651 | 0.000108 | 0.000756 | 0.000587 | 0.002202 | 0.000246 | 0.000582 | 0.000983 | 0.005714 |
None | 0.000626 | 0.001011 | 0.000053 | 0.000374 | 0.002241 | 0.001484 | 0.000059 | 0.000335 | 0.002036 | 0.001297 | 0.000054 | 0.000360 |
Nuclear Periphery | 0.000091 | 0.000093 | 0.000793 | 0.000250 | 0.000089 | 0.000095 | 0.000361 | 0.000180 | 0.000089 | 0.000094 | 0.000668 | 0.000225 |
Nuclear Periphery Foci | 0.001973 | 0.000706 | 0.039926 | 0.006377 | 0.000418 | 0.000718 | 0.018833 | 0.003159 | 0.000615 | 0.000713 | 0.033805 | 0.005234 |
Nucleolus | 0.000122 | 0.000085 | 0.000013 | 0.000076 | 0.000062 | 0.000105 | 0.000052 | 0.000030 | 0.000069 | 0.000097 | 0.000024 | 0.000060 |
Nucleus | 0.003709 | 0.002240 | 0.001267 | 0.004870 | 0.004350 | 0.005391 | 0.007545 | 0.002008 | 0.004269 | 0.004144 | 0.003089 | 0.003853 |
Peroxisomes | 0.174728* | 0.190620* | 0.002043 | 0.035645 | 0.226579* | 0.164696* | 0.028625 | 0.051002 | 0.220005* | 0.174953* | 0.009757 | 0.041103 |
Vacuole | 0.000988 | 0.002395 | 0.021766 | 0.016290 | 0.002010 | 0.003939 | 0.024691 | 0.011596 | 0.001881 | 0.003328 | 0.022614 | 0.014622 |
Vacuole Periphery | 0.000620 | 0.001415 | 0.000304 | 0.002580 | 0.000957 | 0.001419 | 0.000204 | 0.000977 | 0.000914 | 0.001417 | 0.000275 | 0.002010 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.51 | 3.87 | -1.96 | -6.22 | -8.15 | -4.40 | -4.48 | -16.92 | -14.95 | -3.86 | -5.64 | -2.77 | -19.35 | -15.88 | -9.05 |
Bud Neck | -1.90 | -0.90 | -2.95 | -1.25 | -3.36 | -2.67 | 1.27 | -3.11 | -1.18 | -4.09 | -2.17 | 1.71 | -2.67 | -0.66 | -5.31 |
Bud Site | -6.02 | -4.53 | -11.25 | -7.59 | -1.22 | -7.99 | -4.34 | -11.21 | -6.85 | -6.16 | -8.98 | -4.95 | -14.81 | -9.38 | -2.95 |
Cell Periphery | -7.91 | -1.40 | -5.80 | 3.50 | -2.63 | -4.92 | -3.38 | -10.63 | -8.11 | -2.60 | -7.01 | -1.87 | -9.43 | -3.58 | -4.34 |
Cytoplasm | -14.74 | 2.02 | -5.82 | 9.90 | -9.33 | -4.82 | 6.82 | 20.47 | 26.06 | 2.75 | -2.72 | 19.47 | 19.33 | 26.29 | -3.82 |
Cytoplasmic Foci | -0.34 | 5.69 | 0.51 | 1.89 | -12.32 | -2.06 | 0.27 | 12.05 | 14.00 | 3.99 | -1.99 | 8.87 | 5.32 | 8.09 | -5.02 |
Eisosomes | -10.12 | 3.92 | -5.40 | 4.48 | -9.41 | 3.00 | 3.66 | 1.06 | -1.05 | -2.79 | 2.68 | 12.92 | 5.52 | 3.78 | -8.35 |
Endoplasmic Reticulum | -2.99 | -24.93 | -8.67 | -8.27 | 21.17 | -7.54 | -10.00 | -17.43 | -13.89 | 3.13 | -9.18 | -27.66 | -21.52 | -15.61 | 18.54 |
Endosome | 3.35 | -0.29 | -0.71 | -6.90 | -0.48 | -4.08 | -1.94 | -4.30 | -1.80 | -1.21 | -3.38 | -6.80 | -9.98 | -7.99 | -0.40 |
Golgi | -4.77 | 10.23 | 2.28 | 11.35 | -14.64 | 5.22 | 16.77 | 25.62 | 23.54 | 1.28 | 2.67 | 27.83 | 18.05 | 19.06 | -11.55 |
Lipid Particles | 9.86 | 8.84 | 3.42 | -13.43 | -9.13 | 2.81 | -0.18 | -1.10 | -2.97 | -0.37 | 6.68 | 3.09 | -6.74 | -13.44 | -7.50 |
Mitochondria | -2.95 | 5.78 | 4.02 | 9.85 | -5.59 | 0.48 | 17.88 | 11.73 | 11.84 | -2.42 | 0.32 | 18.77 | 14.45 | 16.60 | -5.61 |
Mitotic Spindle | 2.69 | 0.14 | -4.51 | -5.24 | -4.61 | -2.45 | -3.50 | -2.66 | -1.73 | -2.00 | -1.96 | -4.38 | -5.75 | -5.36 | -4.93 |
None | -0.87 | 1.91 | 0.84 | 2.10 | -3.75 | 1.54 | 6.12 | 4.84 | 3.22 | -1.68 | 1.89 | 6.31 | 5.18 | 3.96 | -3.78 |
Nuclear Periphery | -0.02 | -5.75 | -3.70 | -3.36 | 4.22 | -0.43 | -4.89 | -2.26 | -2.17 | 2.84 | -0.32 | -6.57 | -4.35 | -4.20 | 4.79 |
Nuclear Periphery Foci | 2.67 | -8.01 | -4.52 | -6.55 | 6.96 | -4.06 | -5.32 | -5.19 | -4.59 | 4.49 | -1.14 | -9.44 | -7.77 | -7.63 | 7.99 |
Nucleolus | 0.69 | 2.07 | 0.90 | 0.21 | -2.96 | -1.80 | 0.71 | 3.20 | 2.91 | 1.72 | -1.28 | 4.93 | 0.63 | 1.55 | -2.43 |
Nucleus | 1.01 | 1.52 | -0.74 | -4.05 | -5.00 | -2.29 | -2.89 | 5.73 | 6.86 | 4.75 | 0.18 | 1.87 | 0.55 | 0.43 | -1.14 |
Peroxisomes | -1.17 | 13.65 | 10.89 | 27.67* | -17.00 | 9.71 | 29.91* | 29.35* | 22.28 | -4.14 | 7.97 | 43.05* | 35.90* | 37.23* | -14.22 |
Vacuole | -3.67 | -6.66 | -10.71 | -9.75 | 1.78 | -4.01 | -5.54 | -7.24 | -5.64 | 3.19 | -4.14 | -8.26 | -12.25 | -10.79 | 3.17 |
Vacuole Periphery | -4.87 | 2.51 | -3.72 | -2.19 | -4.40 | -2.85 | 6.55 | -0.05 | 1.20 | -2.20 | -3.93 | 6.28 | -2.99 | -1.60 | -4.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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