Standard name
Description Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD4 has a paralog, IMD3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 0.05 0 0.09 0.07 0.08 0 0 0.05 0.05 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.97 0.98 1.0 0.98 0.97 0.93 0.85 0.83 0.86 0.79 0.76 0.76 1.0 0.99 0.99 0.97 0.97 0.97 0.97 0.94 0.88 0.94 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.06 0.09 0.06 0.08 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0.06 0.2 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 9 9 2 5 1 15 0 0 0 0 0 0 0 1 0 0 1 0
Bud 2 1 0 0 0 0 2 2 1 1 1 2 1 0 0 0 0 0 0 0 0 0 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 1 1 1 4 8 19 17 5 22 18 25 4 7 18 19 26 21 0 0 0 0 0 0
Cytoplasm 214 190 29 54 209 214 275 275 149 201 184 227 167 322 355 372 358 317 209 183 60 90 282 314
Endoplasmic Reticulum 1 5 1 0 5 12 20 29 10 19 17 25 2 4 5 10 11 12 1 0 2 0 2 2
Endosome 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 1 0 0 0 1 2 3 0 0 1 3 0 0 0 1 2 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 1 2 14 7 16 15 48 31 0 0 0 2 2 1 0 0 0 0 0 0
Nucleus 1 0 0 0 1 2 4 4 0 5 0 0 1 1 1 0 1 0 0 0 1 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 3 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Vac/Vac Membrane 1 1 0 1 1 4 8 9 1 12 3 14 0 0 1 5 1 2 0 3 0 0 3 2
Unique Cell Count 220 194 29 55 216 231 325 330 174 253 241 300 167 325 359 383 370 328 215 195 68 96 299 330
Labelled Cell Count 227 199 31 56 221 243 356 357 184 282 273 342 175 334 380 409 401 355 215 195 68 96 299 330


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.0 23.2 20.0 19.6 18.8 15.4 13.7 13.1 11.1 11.4 9.8 10.8 23.4 23.7 21.5 32.9 34.0 31.8 19.7 19.2 21.0
Std Deviation (1e-4) 4.0 5.4 4.0 3.4 3.4 3.6 2.9 2.6 3.0 2.6 2.0 2.1 4.4 5.3 5.0 9.1 9.4 9.2 4.8 5.6 5.7
Intensity Change (Log2) – – – -0.03 -0.09 -0.38 -0.54 -0.61 -0.85 -0.8 -1.02 -0.88 0.23 0.24 0.11 0.72 0.77 0.67 -0.02 -0.06 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -1.0 -1.5 -2.3 -2.4 -2.2 -2.7 -2.9 -3.0 0 -0.5 -0.6 -0.9 -1.0 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.3714 15.1012 13.5415 12.4932 11.9577 13.2636 11.1576 18.2544 16.7679 14.2484 19.1769 16.3685 8.9598 15.7712 13.8329 12.8883 12.0224 14.1592
Actin 0.0099 0.0004 0.007 0.0003 0.0029 0.0017 0.0092 0.0008 0.0036 0.0549 0 0.0099 0.038 0.0002 0.0154 0.0025 0.0002 0.0033
Bud 0.0011 0.0005 0.0008 0.0006 0.0004 0.0006 0.0004 0.0018 0.0006 0.0014 0.0001 0.0003 0.0006 0.0035 0.0011 0.0006 0.0002 0.0004
Bud Neck 0.001 0.0004 0.0005 0.0006 0.0006 0.0015 0.0005 0.0003 0.0003 0.0009 0.0004 0.001 0.0024 0.0001 0.0008 0.0013 0.0006 0.0008
Bud Periphery 0.0012 0.0002 0.0004 0.0002 0.0002 0.0006 0.0003 0.0023 0.0005 0.0008 0 0.0001 0.0006 0.006 0.0007 0.0005 0 0.0001
Bud Site 0.0047 0.0021 0.0023 0.0002 0.0005 0.0003 0.0011 0.0026 0.0008 0.0039 0 0.0003 0.0051 0.0005 0.0017 0.0011 0.0001 0.0001
Cell Periphery 0.0007 0.0002 0.0003 0.0001 0.0001 0.0002 0.0004 0.0005 0.0001 0.0006 0 0.0001 0.0005 0.0001 0.0002 0.0002 0 0
Cytoplasm 0.7509 0.9604 0.8777 0.8696 0.7626 0.8619 0.8087 0.9414 0.9299 0.7879 0.9785 0.8877 0.7607 0.9766 0.9046 0.8209 0.8949 0.9076
Cytoplasmic Foci 0.0207 0.0033 0.0108 0.0097 0.0339 0.0142 0.0306 0.0037 0.005 0.0148 0.0045 0.0192 0.0181 0.0027 0.0114 0.0186 0.0205 0.0124
Eisosomes 0.0002 0 0 0 0.0001 0 0 0 0 0.0002 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.044 0.0087 0.0186 0.0043 0.0037 0.0158 0.0589 0.0047 0.0131 0.0043 0.0015 0.0155 0.0472 0.0026 0.0038 0.0157 0.0039 0.015
Endosome 0.0599 0.0064 0.0354 0.0556 0.1084 0.0504 0.0272 0.0055 0.0168 0.051 0.0094 0.0314 0.0459 0.0032 0.021 0.0644 0.067 0.033
Golgi 0.0129 0.0009 0.008 0.0027 0.0088 0.0111 0.009 0.001 0.0035 0.0118 0.0001 0.0166 0.0107 0.0002 0.0055 0.0098 0.0015 0.0124
Lipid Particles 0.0091 0.0005 0.0021 0.0004 0.0183 0.0024 0.0133 0.0002 0.0001 0.0058 0 0.0028 0.0085 0 0.0038 0.0091 0 0.0002
Mitochondria 0.0075 0.0056 0.0068 0.004 0.0309 0.0201 0.0025 0.0046 0.0072 0.0176 0 0.0043 0.0063 0.0002 0.0077 0.0081 0.0002 0.0038
None 0.0133 0.0004 0.0009 0.0004 0.001 0.0005 0.0057 0.018 0.0005 0.0032 0.0002 0.0008 0.0065 0.0004 0.0008 0.0007 0.0004 0.0005
Nuclear Periphery 0.0104 0.002 0.0021 0.0021 0.0019 0.0033 0.0077 0.0006 0.0045 0.0034 0.0006 0.0014 0.0109 0.0004 0.005 0.0069 0.0008 0.0014
Nucleolus 0.0015 0.0001 0.0032 0 0.0001 0.0004 0.0002 0.0001 0 0.0006 0 0 0.0004 0 0.0001 0.0002 0 0
Nucleus 0.0069 0.0018 0.0035 0.0014 0.0014 0.0023 0.0018 0.0009 0.0048 0.002 0.0006 0.001 0.0062 0.0006 0.0026 0.002 0.0008 0.0009
Peroxisomes 0.0122 0.0001 0.0032 0.0001 0.0053 0.001 0.0054 0.0001 0.0001 0.0029 0 0.0025 0.006 0 0.0039 0.0044 0.0001 0.0005
Punctate Nuclear 0.0056 0.0001 0.0005 0.0001 0.0008 0.0014 0.0022 0.0001 0.0001 0.0025 0 0.0003 0.0049 0 0.0015 0.0005 0.0001 0.0001
Vacuole 0.0216 0.0049 0.0132 0.0377 0.0127 0.0072 0.0128 0.0098 0.007 0.0235 0.0033 0.0034 0.0178 0.0021 0.0065 0.0242 0.0064 0.0044
Vacuole Periphery 0.0047 0.001 0.0029 0.0097 0.0055 0.0029 0.0021 0.0011 0.0015 0.006 0.0008 0.0014 0.0023 0.0003 0.0017 0.0082 0.0024 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 426.2994 435.8843 526.9923 424.4387 428.7295 298.2794 349.0634 306.8602 270.608 268.4321
Translational Efficiency 0.8046 0.9661 0.8244 0.8932 0.7785 0.8554 0.8298 0.857 0.745 0.7421

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘