Standard name
Human Ortholog
Description Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25; other members of the complex are Spc1p, Spc1p, and Sec11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0.05 0 0 0.1 0.11 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.3 0.23 0.56 0.66 0.63 0.67 0.72 0.79 0.78 0.82 0.79 0.23 0.16 0.23 0.38 0.34 0.34 0 0 0 0 0 0
Endoplasmic Reticulum – 0.59 0.63 0.34 0.21 0.15 0.16 0.14 0.06 0.12 0.08 0.12 0.73 0.76 0.72 0 0 0 0.63 0.68 0.72 0.87 0.84 0.86
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.17 0.18 0.14 0.19 0.25 0.27 0.19 0.16 0.12 0.11 0.09 0.08 0.05 0 0.52 0.63 0.55 0.2 0.24 0.21 0.07 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 7 7 1 0 9 12 6 8 6 9 4 21 9 7 4 4 0 1 1 0 1 1
Bud – 0 1 1 0 7 0 9 6 12 6 5 0 0 0 1 0 3 0 0 0 0 2 2
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 2 3 5 1 8 15 12 6 11 2 6 42 45 5 0 4 0 0 0 0 1 1
Cytoplasm – 54 34 96 130 136 244 356 250 268 199 190 60 71 96 82 64 74 0 5 2 0 4 2
Endoplasmic Reticulum – 105 92 59 42 32 58 70 19 43 20 29 188 329 303 3 2 7 24 150 116 257 273 360
Endosome – 0 1 3 0 4 3 0 2 1 3 2 6 5 2 1 2 1 1 4 2 3 0 3
Golgi – 1 0 0 0 0 0 1 0 0 0 1 1 4 2 16 5 8 0 1 0 5 3 1
Mitochondria – 0 1 0 0 2 0 0 2 1 1 0 0 0 0 2 3 7 2 1 1 1 3 1
Nucleus – 1 2 2 2 5 14 7 0 1 0 5 0 0 0 3 11 12 0 0 0 0 0 0
Nuclear Periphery – 1 0 0 0 0 0 0 0 0 0 0 10 13 9 0 1 0 0 2 1 3 2 3
Nucleolus – 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 1 1 1 1 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 31 27 24 38 54 99 95 51 41 26 21 20 20 12 112 118 120 7 51 33 19 31 38
Unique Cell Count 178 147 172 196 215 364 495 318 345 243 240 257 432 421 215 188 219 39 219 162 295 327 420
Labelled Cell Count 194 167 195 218 242 435 565 348 383 273 265 296 505 478 233 212 241 39 219 162 295 327 420


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 15.5 14.5 13.8 15.0 9.5 12.1 11.1 10.4 9.9 9.5 9.8 14.1 13.4 14.4 19.9 18.5 17.5 14.9 15.4 16.2
Std Deviation (1e-4) – 2.8 2.4 2.0 2.5 2.4 2.0 1.8 5.8 1.8 1.7 1.8 3.2 2.9 2.7 5.1 5.0 4.7 2.4 3.2 3.3
Intensity Change (Log2) – – – -0.08 0.04 -0.62 -0.26 -0.38 -0.49 -0.56 -0.61 -0.57 -0.05 -0.11 -0.01 0.45 0.35 0.26 0.04 0.08 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 0 0 0 0 0 0 0 -0.5 0 0 0 -0.7 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 3.3 3.5 0 0 0
Cytoplasm 5.9 7.9 7.5 9.0 10.6 11.4 11.4 11.5 10.8 0 -1.8 -0.1 3.0 2.2 2.2
Endoplasmic Reticulum -5.0 -7.7 -9.4 -10.5 -11.9 -13.3 -11.4 -11.5 -10.4 2.2 3.2 2.1 -13.0 -12.4 -12.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 2.1 2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.1 0.2 1.5 2.1 0.2 -0.6 -1.9 -2.1 -2.8 -3.2 -5.3 -6.4 6.5 8.1 7.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5703 7.7498 7.3398 6.8483 7.3183 7.2323 10.3637 12.1746 11.6425 10.5632 10.3944 11.6791 10.791 12.7809 11.3315 10.9211 10.7818 11.961
Actin 0.0003 0.0001 0.0001 0.0305 0.0001 0.0002 0.0071 0 0.0002 0 0.0049 0.0004 0.0224 0.0011 0.0192 0.0076 0.0008 0.0004
Bud 0 0.0001 0 0.0001 0 0 0.0001 0 0 0 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0
Bud Neck 0.0001 0.0003 0.0002 0.0007 0.0005 0.0004 0.0006 0.0002 0.0002 0.0001 0.0022 0.0003 0.0055 0.0005 0.001 0.0005 0.0012 0.0005
Bud Periphery 0.0001 0.0001 0 0.0002 0 0.0001 0.0002 0 0.0001 0.0001 0.0003 0 0.0007 0.0002 0.0002 0.0002 0.0003 0.0001
Bud Site 0.0001 0.0008 0 0.0006 0.0001 0.0001 0.0002 0.0004 0.0001 0 0.0001 0 0.0018 0.0006 0.001 0.0006 0.0003 0
Cell Periphery 0.0014 0.0032 0.0009 0.0003 0.0003 0.0006 0.0026 0.0019 0.001 0.001 0.0015 0.0004 0.0042 0.0031 0.0023 0.0006 0.0011 0.0016
Cytoplasm 0.0344 0.0667 0.0212 0.0058 0.0431 0.0182 0.0095 0.0298 0.0255 0.0071 0.0257 0.0202 0.0163 0.0261 0.0124 0.0127 0.0207 0.0217
Cytoplasmic Foci 0.0078 0.0012 0.001 0.0008 0.001 0.0015 0.0012 0.0008 0.0007 0.0001 0.0007 0.0006 0.0075 0.0045 0.0068 0.0121 0.0028 0.0008
Eisosomes 0 0 0 0.0006 0 0 0.0001 0 0 0 0.0002 0 0.0003 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.8023 0.8405 0.915 0.9143 0.8814 0.8883 0.7704 0.8924 0.9036 0.9311 0.8177 0.9037 0.6953 0.8595 0.8626 0.8394 0.7997 0.8959
Endosome 0.0289 0.0125 0.0127 0.009 0.0099 0.013 0.0242 0.0054 0.0082 0.005 0.0149 0.0103 0.0386 0.019 0.0074 0.0261 0.026 0.0101
Golgi 0.0007 0.0007 0.0004 0.0025 0.0002 0.0006 0.0026 0.0002 0.0006 0.0001 0.0027 0.001 0.0128 0.0031 0.004 0.0129 0.0057 0.0006
Lipid Particles 0.0095 0.006 0.0037 0.0031 0.0018 0.0074 0.0145 0.0059 0.0019 0.0004 0.0054 0.0022 0.0412 0.015 0.0144 0.0235 0.0146 0.0091
Mitochondria 0.0004 0.0002 0.0001 0.0034 0.0001 0.0002 0.0068 0 0.0041 0.0001 0.0016 0.0006 0.0126 0.0002 0.0168 0.0071 0.0354 0.0002
None 0.0011 0.0002 0.0001 0.0002 0.0001 0.0001 0.0011 0.0001 0.0001 0 0.001 0.0001 0.0044 0.0006 0.0009 0.0011 0.0002 0.0002
Nuclear Periphery 0.0585 0.0195 0.014 0.0115 0.035 0.0392 0.0779 0.0209 0.0212 0.0191 0.0414 0.0362 0.0584 0.0109 0.0152 0.0144 0.0465 0.0283
Nucleolus 0.0001 0.0001 0 0.0001 0 0.0001 0.0003 0.0002 0 0 0.0002 0 0.0028 0.0001 0.0002 0.0001 0.0002 0
Nucleus 0.0039 0.0019 0.0008 0.0006 0.0042 0.0039 0.0058 0.0038 0.0016 0.0009 0.0032 0.0028 0.0097 0.0011 0.0008 0.0008 0.0037 0.0016
Peroxisomes 0.0002 0 0 0.0017 0 0 0.0002 0 0 0 0.0001 0 0.0059 0.0001 0.0047 0.0165 0.0022 0
Punctate Nuclear 0.0007 0.0001 0.0001 0.0002 0.0003 0.0004 0.001 0.0005 0.0001 0 0.0006 0.0002 0.0049 0.0009 0.0013 0.0013 0.0003 0.0004
Vacuole 0.0462 0.0443 0.0282 0.0125 0.0202 0.0236 0.0674 0.0365 0.0279 0.0312 0.0717 0.0189 0.0479 0.0519 0.0268 0.0204 0.0358 0.0265
Vacuole Periphery 0.0033 0.0014 0.0014 0.0011 0.0015 0.0023 0.0062 0.001 0.0028 0.0035 0.0038 0.0019 0.0064 0.0014 0.0021 0.0019 0.0025 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.2047 27.5524 34.0751 44.2121 29.3049 59.555 33.4299 40.1118 51.6908 55.276
Translational Efficiency 1.6715 2.2754 1.4759 1.4977 2.2585 1.598 2.0216 1.366 1.2681 1.4534

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘