Standard name
Description AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.98 0.93 0.92 0.82 0.81 0.76 0.64 0.64 0.6 0.98 0.98 0.98 0.96 0.95 0.99 0.95 0.91 0.91 0.82 0.78 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.05 0 0 0.07 0.07 0 0 0 0 0.09 0 0 0 0 0.11 0.13 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.13 0.13 0.27 0.34 0.33 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0.05 0.11 0.08 0.06 0 0 0 0 0 0 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 4 6 4 4 7 3 0 0 1 0 1 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 1 0 1 1 4 3 3 0 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 2 3 4 10 0 4 5 6 5 7 7 4 7 6 6 4 3 0 0 0 2 0 0
Cytoplasm 256 211 172 166 211 98 176 153 123 116 149 123 250 352 387 78 74 82 256 207 171 184 250 251
Endoplasmic Reticulum 7 3 2 2 5 1 10 10 3 4 17 15 2 5 6 3 7 2 4 1 4 23 41 21
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 2 0 1 0 1 0 0 3 0 0 0 1 0
Mitochondria 1 1 0 0 4 15 27 24 43 61 78 73 2 0 1 0 2 1 0 3 5 2 4 2
Nucleus 2 0 0 2 2 2 3 2 2 8 3 6 1 1 2 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 0 1 4 2 7 12 8 20 18 12 1 1 2 1 0 1 1 12 2 8 12 16
Unique Cell Count 264 216 174 169 227 107 215 190 162 180 233 204 255 359 395 81 78 83 271 228 189 226 320 300
Labelled Cell Count 269 218 177 176 237 118 232 212 190 220 283 245 263 367 405 90 88 89 271 228 189 226 320 300


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 10.2 10.0 9.2 9.6 7.6 8.6 8.7 7.8 7.4 7.5 7.6 11.6 12.4 12.4 18.0 18.6 17.6 10.5 10.5 11.3
Std Deviation (1e-4) 1.7 2.0 1.6 1.5 1.3 1.5 1.6 1.5 1.7 1.7 1.6 1.4 1.9 2.0 2.1 3.6 4.0 2.6 1.5 2.0 2.0
Intensity Change (Log2) -0.12 -0.05 -0.39 -0.22 -0.2 -0.35 -0.44 -0.42 -0.39 0.22 0.31 0.31 0.85 0.9 0.82 0.07 0.07 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.5 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -2.8 -3.0 -5.4 -5.6 -6.4 -8.3 -8.5 -9.0 -0.7 -0.7 -0.7 -1.4 -1.9 0
Endoplasmic Reticulum 0 0 0 2.0 2.2 0 0 2.9 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.1 4.8 4.9 7.3 8.4 8.5 8.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.4 0 4.5 3.8 3.3 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Amd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available