Standard name
Description Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.08 0.08 0.13 0.1 0.17 0.18 0.21
Bud 0.07 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0.08 0.07 0 0 0 0 0 0 0 0.08 0 0 0.09 0.07 0 0 0.05 0.08 0.11 0.14 0.14
Cytoplasm 0.52 0.67 0.92 0.87 0.84 0.84 0.84 0.92 0.77 0.66 0.61 0.65 0.86 0.91 0.96 0.75 0.7 0.62 0.14 0.2 0.19 0.13 0.07 0.07
Endoplasmic Reticulum 0.37 0.21 0 0 0 0.05 0 0 0 0 0 0 0.1 0.06 0 0.08 0 0.12 0.47 0.27 0.2 0.23 0.26 0.24
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0.12 0.1 0.13 0.18 0.13 0.36 0.39 0.61 0.5 0 0 0 0.12 0.05 0.15 0.14 0.19 0.29 0.21 0.18 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.12 0.06 0.05 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 16 3 2 1 2 2 0 0 0 0 0 1 2 1 2 16 2 10 36 19 24 51 47
Bud 6 10 2 1 2 1 4 1 0 0 4 0 2 1 1 0 7 0 1 2 2 1 3 1
Bud Neck 1 1 2 0 1 1 0 3 0 0 0 0 0 0 1 0 4 0 2 7 5 4 11 13
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 7 10 10 11 3 3 4 0 3 0 1 12 5 8 6 6 1 5 13 16 16 40 31
Cytoplasm 45 142 153 113 137 139 203 216 92 85 49 30 126 199 272 49 57 16 17 57 38 18 20 16
Endoplasmic Reticulum 32 45 2 2 7 8 7 1 3 4 0 0 15 14 4 5 2 3 60 77 39 32 73 55
Endosome 0 1 0 0 0 2 2 0 0 0 0 0 2 0 0 0 0 2 2 9 2 1 4 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1 0 1 4
Mitochondria 12 7 5 16 17 22 44 31 43 50 49 23 3 3 1 8 4 4 17 55 57 29 49 34
Nucleus 0 2 0 1 0 1 3 2 3 2 1 0 0 0 2 2 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 3 0 0 0 1 1 0 0 0 0 1 2 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 1 0 1 1 1 2 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 0 6 2 1 2 1 1 2 1 2 0 0 0 1 2 3 2 3 7 12 7 4 14 11
Unique Cell Count 87 212 167 130 163 166 241 236 120 128 80 46 146 218 283 65 81 26 128 285 199 141 280 229
Labelled Cell Count 103 237 180 146 179 181 272 262 143 150 104 54 161 227 294 76 101 31 128 285 199 141 280 229


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.9 5.3 5.1 5.5 4.4 4.6 4.8 4.3 4.3 3.8 3.9 5.1 5.2 5.1 6.7 6.9 6.3 4.7 4.9 5.5
Std Deviation (1e-4) 0.6 0.7 0.7 1.4 1.1 1.2 1.4 1.7 1.9 1.9 1.7 0.8 1.1 1.3 1.5 1.5 1.6 1.4 1.1 1.6 1.8
Intensity Change (Log2) -0.04 0.05 -0.27 -0.21 -0.14 -0.3 -0.28 -0.48 -0.43 -0.04 -0.02 -0.03 0.35 0.38 0.25 -0.17 -0.1 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 5.0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 0.3 0 0 0 0 0 0 0 0.8 -1.9 -1.7 0 0.4 0
Cytoplasm -1.3 -2.1 -2.2 -2.2 0 -3.5 -5.4 -5.8 -4.6 -1.5 -0.1 2.0 -3.3 -4.4 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 3.5 2.5 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Usa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available