Standard name
Description Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.94 0.95 0.96 0.88 0.81 – 0.77 0.7 0.71 0.64 0.99 0.98 0.97 0.98 0.97 0.98 0.94 0.96 0.91 0.95 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 – 0.13 0.15 0.22 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0.16 – 0.13 0.12 0.08 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 0 2 2 1
Bud 0 1 0 0 0 2 0 – 1 3 2 1 0 0 0 0 0 0 3 3 0 2 11 13
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 1 1 3 0 5 – 6 11 2 0 1 3 10 3 8 4 0 0 0 0 0 0
Cytoplasm 262 371 87 81 103 119 163 – 144 167 85 54 359 582 658 216 174 200 254 357 98 348 520 510
Endoplasmic Reticulum 1 0 1 0 0 1 5 – 2 8 0 1 1 2 4 11 6 3 0 0 2 3 5 4
Endosome 2 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0
Mitochondria 1 0 0 2 1 16 4 – 24 35 26 23 0 0 0 2 1 2 0 0 0 0 0 1
Nucleus 5 2 4 0 1 3 4 – 4 11 6 2 4 6 6 0 0 0 1 2 2 0 0 3
Nuclear Periphery 0 0 0 1 0 1 0 – 0 0 0 5 1 0 0 0 0 0 2 0 0 1 2 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 5 0 3 2 3 11 32 – 25 30 10 10 3 7 7 2 4 3 1 2 0 4 3 4
Unique Cell Count 271 372 93 85 107 136 201 186 240 119 85 364 594 675 221 180 205 270 373 108 367 556 547
Labelled Cell Count 281 376 96 87 111 153 213 206 266 131 98 369 600 685 234 195 213 270 373 108 367 556 547


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.4 18.0 18.0 14.6 14.8 8.1 10.2 – 8.1 8.1 7.1 6.8 17.5 16.8 16.4 23.8 25.1 22.9 18.0 17.9 18.1
Std Deviation (1e-4) 6.0 4.5 4.5 4.4 3.6 2.6 2.2 – 1.8 1.8 1.8 1.4 4.7 4.1 4.0 5.4 5.8 5.8 4.7 5.1 5.2
Intensity Change (Log2) – – – -0.3 -0.28 -1.15 -0.83 – -1.15 -1.15 -1.35 -1.41 -0.04 -0.11 -0.14 0.4 0.48 0.34 -0.01 -0.01 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.9 -1.5 -2.8 – -3.4 -4.6 -4.1 -4.9 2.9 2.5 2.1 1.8 1.2 1.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 0 – 3.6 3.9 4.8 5.4 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.5 3.1 – 2.7 2.5 1.6 2.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.2203 11.7702 11.0653 10.4769 9.9973 10.8177 13.9799 16.803 16.091 14.2909 16.1118 15.782 15.8295 19.1351 17.8384 14.0176 17.5468 17.274
Actin 0.0031 0.0011 0.0012 0.0005 0.0002 0.0003 0.0015 0.0009 0.0007 0.0013 0.0006 0.001 0.0056 0.0004 0.0005 0.0013 0.0009 0.0009
Bud 0.0003 0.0007 0.0018 0.0002 0.0003 0.0001 0.0019 0.0016 0.0021 0.0006 0.0007 0.0004 0.0003 0.0009 0.0013 0.0004 0.0027 0.0002
Bud Neck 0.0005 0.0003 0.0003 0.0007 0.0006 0.0012 0.0007 0.0004 0.0008 0.001 0.0009 0.0015 0.0004 0.0002 0.0003 0.0005 0.0004 0.0012
Bud Periphery 0.0002 0.0004 0.0009 0 0.0001 0 0.0011 0.0006 0.0009 0.0003 0.0005 0.0004 0.0001 0.001 0.0009 0.0001 0.0019 0.0001
Bud Site 0.0004 0.0043 0.0031 0.0001 0 0 0.0014 0.0019 0.004 0.0015 0.0002 0.0001 0.0005 0.0002 0.0002 0.0003 0.0003 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0
Cytoplasm 0.9344 0.9769 0.972 0.9731 0.9819 0.9684 0.9496 0.9575 0.9373 0.9347 0.9639 0.9528 0.9436 0.9858 0.9795 0.9682 0.9798 0.9656
Cytoplasmic Foci 0.0095 0.0024 0.0016 0.0023 0.0023 0.0024 0.002 0.0017 0.002 0.0031 0.0016 0.0026 0.0097 0.0009 0.0011 0.0025 0.0011 0.0042
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0032 0.0007 0.0007 0.0005 0.0008 0.002 0.003 0.0026 0.0019 0.0023 0.0018 0.0017 0.0028 0.0006 0.0007 0.0046 0.0013 0.0017
Endosome 0.0098 0.0004 0.0004 0.0008 0.0006 0.0015 0.001 0.0006 0.001 0.0006 0.0009 0.0011 0.0021 0.0003 0.0003 0.0012 0.0004 0.0017
Golgi 0.0029 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0015 0 0 0.0001 0 0.0001
Lipid Particles 0.0033 0 0 0 0 0 0 0 0 0 0 0 0.0034 0 0 0 0 0.0001
Mitochondria 0.0071 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
None 0.008 0.003 0.0024 0.0018 0.0015 0.0014 0.0058 0.0025 0.0047 0.0048 0.0025 0.0022 0.0099 0.0013 0.002 0.0085 0.0017 0.0044
Nuclear Periphery 0.0026 0.0011 0.0014 0.0013 0.0014 0.0024 0.0036 0.0029 0.0033 0.0042 0.0025 0.0024 0.002 0.0007 0.001 0.0024 0.0017 0.0016
Nucleolus 0.0001 0 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Nucleus 0.0098 0.007 0.0128 0.0173 0.009 0.0185 0.0248 0.0243 0.0369 0.0411 0.02 0.031 0.0105 0.0067 0.0111 0.0077 0.0067 0.0148
Peroxisomes 0.0014 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0004 0 0.0001 0.0053 0 0 0 0 0.0001
Punctate Nuclear 0.0015 0.0008 0.0007 0.0008 0.0003 0.0006 0.0008 0.001 0.0015 0.0025 0.0019 0.0015 0.0011 0.0003 0.0003 0.0007 0.0003 0.0017
Vacuole 0.0013 0.0004 0.0003 0.0003 0.0005 0.0007 0.0018 0.0011 0.0017 0.0012 0.0017 0.0008 0.0007 0.0005 0.0004 0.001 0.0005 0.0013
Vacuole Periphery 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 259.2218 279.823 266.0397 208.896 195.3141 238.5476 209.9862 173.0556 194.996 189.717
Translational Efficiency 1.3675 1.3028 1.3952 1.6614 1.8059 1.4649 1.564 1.9964 1.5511 1.7433

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘