ORF
Description Putative protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.79 0.91 0.95 0.9 0.75 0.69 – 0.57 0.64 0.58 0.57 0.97 0.96 0.98 0.81 0.77 0.75 0.32 0.4 0.33 0.56 0.61 0.67
Endoplasmic Reticulum 0 0.09 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0.1 0.09 – 0.07 0.1 0.05 0.1 0 0 0 0.07 0.07 0.09 0.06 0.07 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.09 0 0 0 0.05 0.12 – 0.28 0.21 0.39 0.31 0.08 0 0 0 0 0 0.35 0.27 0.36 0.2 0.11 0.11
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.07 0.12 0.1 – 0.1 0.11 0.1 0.09 0 0 0 0.09 0.16 0.17 0.16 0.15 0.13 0.16 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 1 – 1 0 0 0 0 0 0 2 2 2 0 2 4 0 0 1
Bud 0 0 0 0 0 2 6 – 6 4 7 3 1 0 0 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 2 0 1 1 4 – 0 3 0 1 0 2 0 0 1 0 1 1 2 0 1 1
Bud Site 0 0 0 0 0 0 0 – 1 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 0 0 3 – 1 3 0 0 0 0 0 4 4 1 0 0 0 0 0 0
Cytoplasm 80 44 75 161 234 178 226 – 123 133 151 147 37 74 107 198 193 177 40 44 36 13 34 81
Endoplasmic Reticulum 1 5 1 2 0 4 1 – 2 6 1 2 0 1 0 9 9 14 9 3 2 0 1 1
Endosome 0 1 0 2 10 24 30 – 16 21 12 27 0 0 1 16 17 22 8 8 2 0 0 1
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 1 0 3 0 1 1 0 0 0
Mitochondria 7 5 0 2 3 13 38 – 60 44 100 80 3 0 0 6 4 7 44 30 40 4 6 13
Nucleus 0 0 0 0 0 0 6 – 4 0 4 4 1 0 0 2 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 – 0 0 1 2 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 1 4 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 4 3 8 – 11 5 2 13 0 0 0 4 3 0 1 1 0 0 0 0
Vac/Vac Membrane 0 3 1 5 17 29 31 – 22 22 25 24 0 0 0 22 39 40 20 17 14 3 7 14
Unique Cell Count 86 56 82 169 261 237 326 217 209 259 258 38 77 109 243 250 237 128 113 112 24 56 121
Labelled Cell Count 88 60 83 174 272 255 358 247 242 304 307 42 78 109 265 276 266 128 113 112 24 56 121


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 5.8 5.5 6.2 5.4 5.3 – 4.7 5.0 4.6 4.9 4.6 5.3 5.6 7.1 7.2 7.2 – 5.7 5.3
Std Deviation (1e-4) 0.4 1.0 1.0 1.6 1.9 2.5 1.5 – 1.4 1.4 1.1 1.1 0.5 0.9 1.7 1.3 1.3 1.6 – 1.8 1.1
Intensity Change (Log2) – – – -0.07 0.11 -0.09 -0.13 – -0.29 -0.2 -0.34 -0.25 -0.34 -0.12 -0.05 0.31 0.32 0.31 – -0.02 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.4 3.5 3.7