| Standard name | |
|---|---|
| Description | tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
| Bud | 0 | 3 | 0 | 1 | 2 | 2 | 1 | 2 | 8 | 7 | 13 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 |
| Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 1 | 2 | 9 | 6 | 8 | 4 | 7 | 7 | 3 | 8 | 4 | 7 | 2 | 0 | 1 | 3 | 11 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 95 | 162 | 285 | 230 | 413 | 364 | 403 | 436 | 351 | 346 | 336 | 336 | 157 | 221 | 257 | 129 | 131 | 155 | 104 | 169 | 268 | 143 | 228 | 218 |
| Endoplasmic Reticulum | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 9 | 8 | 10 | 0 | 0 | 2 | 1 | 0 | 1 |
| Endosome | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 1 | 6 | 3 | 3 | 3 | 0 | 2 | 0 | 2 | 2 | 3 | 0 | 0 | 2 | 0 | 0 | 2 |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
| Mitochondria | 2 | 10 | 1 | 10 | 5 | 50 | 78 | 56 | 169 | 114 | 169 | 142 | 0 | 0 | 0 | 5 | 2 | 3 | 2 | 2 | 6 | 2 | 1 | 2 |
| Nucleus | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 1 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 3 |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 13 | 2 | 1 | 3 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| Vac/Vac Membrane | 0 | 0 | 5 | 3 | 11 | 10 | 4 | 15 | 13 | 7 | 9 | 16 | 1 | 0 | 0 | 4 | 3 | 5 | 1 | 0 | 2 | 4 | 3 | 0 |
| Unique Cell Count | 97 | 167 | 289 | 233 | 427 | 391 | 461 | 503 | 444 | 417 | 448 | 449 | 160 | 225 | 258 | 133 | 139 | 166 | 110 | 178 | 295 | 161 | 244 | 238 |
| Labelled Cell Count | 100 | 177 | 300 | 253 | 446 | 435 | 506 | 540 | 556 | 490 | 542 | 516 | 162 | 227 | 260 | 153 | 160 | 182 | 110 | 178 | 295 | 161 | 244 | 238 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 5.8 | 4.5 | 6.9 | 6.3 | 6.7 | 5.4 | 5.5 | 6.1 | 4.6 | 4.7 | 4.8 | 4.9 | 6.5 | 6.4 | 6.4 | 6.4 | 7.9 | 8.3 | 7.8 | 7.7 | 7.7 |
| Std Deviation (1e-4) | 0.7 | 0.6 | 1.6 | 1.3 | 1.6 | 1.3 | 2.5 | 4.6 | 1.8 | 1.3 | 2.0 | 1.9 | 1.6 | 1.5 | 1.5 | 1.4 | 1.5 | 1.6 | 2.5 | 2.2 | 2.3 |
| Intensity Change (Log2) | – | – | – | -0.12 | -0.04 | -0.34 | -0.33 | -0.16 | -0.58 | -0.55 | -0.52 | -0.5 | -0.09 | -0.1 | -0.09 | -0.1 | 0.2 | 0.27 | 0.18 | 0.16 | 0.17 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
