Standard name
Description tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.99 0.97 0.93 0.87 0.87 0.79 0.83 0.75 0.75 0.98 0.98 1.0 0.97 0.94 0.93 0.95 0.95 0.91 0.89 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.13 0.17 0.11 0.38 0.27 0.38 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud 0 3 0 1 2 2 1 2 8 7 13 2 0 1 0 0 0 0 0 0 1 1 1 2
Bud Neck 0 0 0 0 1 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 9 6 8 4 7 7 3 8 4 7 2 0 1 3 11 3 0 0 0 0 0 0
Cytoplasm 95 162 285 230 413 364 403 436 351 346 336 336 157 221 257 129 131 155 104 169 268 143 228 218
Endoplasmic Reticulum 0 0 0 3 0 0 0 0 0 0 0 0 1 0 0 9 8 10 0 0 2 1 0 1
Endosome 0 0 0 0 1 4 2 1 6 3 3 3 0 2 0 2 2 3 0 0 2 0 0 2
Golgi 0 0 0 0 0 0 0 1 0 0 1 0 1 1 0 0 2 1 0 1 0 0 1 0
Mitochondria 2 10 1 10 5 50 78 56 169 114 169 142 0 0 0 5 2 3 2 2 6 2 1 2
Nucleus 2 0 0 0 2 0 2 3 1 2 3 1 0 0 1 0 0 2 0 0 0 0 2 3
Nuclear Periphery 0 0 0 0 1 0 0 2 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 2 13 2 1 3 3 0 1 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 5 0 2 0 1 3 0 0 0 1 1 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 5 3 11 10 4 15 13 7 9 16 1 0 0 4 3 5 1 0 2 4 3 0
Unique Cell Count 97 167 289 233 427 391 461 503 444 417 448 449 160 225 258 133 139 166 110 178 295 161 244 238
Labelled Cell Count 100 177 300 253 446 435 506 540 556 490 542 516 162 227 260 153 160 182 110 178 295 161 244 238


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.5 6.9 6.3 6.7 5.4 5.5 6.1 4.6 4.7 4.8 4.9 6.5 6.4 6.4 6.4 7.9 8.3 7.8 7.7 7.7
Std Deviation (1e-4) 0.7 0.6 1.6 1.3 1.6 1.3 2.5 4.6 1.8 1.3 2.0 1.9 1.6 1.5 1.5 1.4 1.5 1.6 2.5 2.2 2.3
Intensity Change (Log2) – – – -0.12 -0.04 -0.34 -0.33 -0.16 -0.58 -0.55 -0.52 -0.5 -0.09 -0.1 -0.09 -0.1 0.2 0.27 0.18 0.16 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available