Standard name
Description Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0.15 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.14 0.18 0.22 0.16 0.14 0.06 0.13 0.08 0.1 0.17 0.23 0.1 0.1 0.17 0 0 0 0 0.09 0.19
Endoplasmic Reticulum 0.74 0.74 0.6 0.8 0.71 0.48 0.33 0.26 0.07 0.13 0.72 0.58 0 0 0.05 0.84 0.82 0.78 0.57 0.44 0.43
Endosome 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.07 0 0 0 0 0 0 0 0.06 0 0.66 0.52 0.43 0 0 0 0 0.06 0
Mitochondria 0.16 0 0 0 0.06 0.08 0.29 0.24 0.59 0.39 0 0 0.06 0.06 0.05 0 0 0 0.19 0.12 0
Nucleus 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.15 0.18 0.12 0.11 0.4 0.31 0.48 0.27 0.38 0.17 0.21 0.35 0.43 0.4 0 0 0.07 0 0.13 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 7 2 1 3 2 1 0 0 0 0 0 9 11 8 6 2 0 1 0 0
Bud 1 1 0 1 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3 0 1 3 3 0 1 0 0 0 0 9 7 5 0 0 0 0 0 0
Cytoplasm 19 21 8 11 13 15 3 12 5 8 3 10 28 20 22 1 3 0 0 4 12
Endoplasmic Reticulum 105 111 27 41 57 51 16 23 4 10 13 25 8 6 6 150 134 16 4 21 28
Endosome 3 0 0 0 1 0 0 1 1 1 1 0 2 6 3 3 3 0 0 2 1
Golgi 3 6 3 1 2 1 1 1 0 0 1 0 185 104 56 2 5 0 0 2 0
Mitochondria 22 3 2 0 5 8 14 21 35 31 0 0 17 11 7 5 5 0 1 5 1
Nucleus 0 0 0 0 0 1 3 4 1 3 0 1 2 1 0 0 0 0 0 0 1
Nuclear Periphery 8 0 0 1 0 0 2 3 2 2 0 0 4 0 1 0 0 0 0 1 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 13 22 8 6 9 42 15 43 16 30 3 9 100 86 52 7 6 1 0 6 14
Unique Cell Count 141 150 45 51 80 106 48 89 59 79 18 43 282 200 129 180 165 21 8 49 66
Labelled Cell Count 180 174 51 63 93 124 55 110 64 85 21 45 365 252 163 180 165 21 8 49 66


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 11.3 9.0 10.3 8.6 8.7 7.6 7.7 7.2 7.4 11.4 10.9 18.2 16.9 16.3 9.9
Std Deviation (1e-4) 1.6 2.1 1.3 1.3 1.3 1.4 1.2 1.3 1.2 1.2 1.2 1.5 4.5 3.4 4.7 1.4
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubx2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available