| Standard name | |
|---|---|
| Description | Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 1 | 1 | 7 | 2 | 7 | 0 | 3 | 2 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | – | 0 | 2 | 4 | 2 | 6 | 4 |
| Bud | 0 | 0 | 3 | 5 | 15 | 7 | 13 | 15 | 18 | 17 | 22 | 13 | 3 | 1 | 0 | 0 | 0 | – | 10 | 14 | 23 | 4 | 6 | 5 |
| Bud Neck | 0 | 0 | 18 | 12 | 29 | 14 | 22 | 29 | 15 | 13 | 10 | 14 | 0 | 1 | 1 | 0 | 0 | – | 1 | 8 | 15 | 5 | 4 | 7 |
| Bud Site | 0 | 1 | 1 | 3 | 1 | 1 | 2 | 1 | 5 | 6 | 8 | 9 | 2 | 1 | 1 | 0 | 0 | – | – | – | – | – | – | – |
| Cell Periphery | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 4 | 0 | 0 | 1 | 0 | 2 | 0 | – | 0 | 0 | 3 | 0 | 5 | 5 |
| Cytoplasm | 105 | 194 | 286 | 302 | 303 | 334 | 327 | 391 | 307 | 417 | 322 | 328 | 265 | 388 | 333 | 57 | 59 | – | 63 | 129 | 155 | 126 | 293 | 270 |
| Endoplasmic Reticulum | 3 | 4 | 8 | 1 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | – | 1 | 0 | 2 | 1 | 8 | 2 |
| Endosome | 12 | 12 | 3 | 1 | 2 | 2 | 0 | 2 | 1 | 2 | 5 | 6 | 18 | 61 | 50 | 12 | 15 | – | 16 | 16 | 17 | 3 | 1 | 3 |
| Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | – | 2 | 2 | 0 | 0 | 1 | 0 |
| Mitochondria | 8 | 34 | 1 | 13 | 10 | 58 | 91 | 47 | 169 | 212 | 206 | 137 | 21 | 10 | 16 | 5 | 4 | – | 22 | 18 | 42 | 28 | 67 | 38 |
| Nucleus | 8 | 25 | 26 | 14 | 24 | 27 | 51 | 44 | 45 | 64 | 54 | 64 | 9 | 10 | 25 | 3 | 2 | – | 7 | 28 | 15 | 1 | 0 | 2 |
| Nuclear Periphery | 3 | 2 | 3 | 6 | 1 | 8 | 6 | 3 | 10 | 11 | 5 | 5 | 4 | 6 | 2 | 0 | 1 | – | 10 | 10 | 14 | 3 | 2 | 1 |
| Nucleolus | 17 | 5 | 3 | 6 | 13 | 9 | 9 | 2 | 16 | 16 | 31 | 17 | 17 | 27 | 21 | 3 | 6 | – | 2 | 4 | 3 | 1 | 0 | 0 |
| Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 1 | 4 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 |
| SpindlePole | 15 | 11 | 26 | 30 | 90 | 26 | 37 | 38 | 27 | 33 | 23 | 48 | 15 | 12 | 13 | 2 | 11 | – | 42 | 64 | 90 | 5 | 4 | 2 |
| Vac/Vac Membrane | 15 | 29 | 13 | 7 | 11 | 10 | 5 | 17 | 5 | 8 | 14 | 16 | 7 | 14 | 14 | 22 | 14 | – | 10 | 18 | 24 | 8 | 16 | 10 |
| Unique Cell Count | 163 | 293 | 382 | 370 | 456 | 452 | 493 | 535 | 502 | 664 | 583 | 570 | 340 | 493 | 453 | 98 | 98 | 199 | 328 | 434 | 196 | 433 | 358 | |
| Labelled Cell Count | 189 | 318 | 401 | 403 | 510 | 501 | 567 | 593 | 620 | 804 | 705 | 660 | 365 | 534 | 482 | 112 | 120 | 199 | 328 | 434 | 196 | 433 | 358 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.3 | 3.9 | 4.7 | 4.3 | 4.9 | 3.7 | 3.8 | 4.2 | 3.4 | 3.3 | 3.3 | 3.8 | 3.9 | 4.0 | 4.2 | 6.4 | 6.2 | – | 3.7 | 3.5 | 3.8 |
| Std Deviation (1e-4) | 1.0 | 1.0 | 0.9 | 1.1 | 1.2 | 1.0 | 1.0 | 1.1 | 1.1 | 0.9 | 1.0 | 1.0 | 1.0 | 1.2 | 1.5 | 2.0 | 1.7 | – | 1.2 | 1.3 | 0.9 |
| Intensity Change (Log2) | – | – | – | -0.15 | 0.06 | -0.36 | -0.3 | -0.19 | -0.49 | -0.54 | -0.5 | -0.31 | -0.27 | -0.24 | -0.17 | 0.43 | 0.4 | – | -0.37 | -0.42 | -0.32 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.5 | 4.2 | – |
External localization resources
