Standard name
Human Ortholog
Description Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0
Bud Neck 0 0 0.05 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.64 0.66 0.75 0.82 0.66 0.74 0.66 0.73 0.61 0.63 0.55 0.58 0.78 0.79 0.74 0.58 0.6 0.32 0.39 0.36 0.65 0.68 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0.11 0.12 0.15 0.08 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.12 0 0 0 0.13 0.18 0.09 0.34 0.32 0.35 0.24 0.06 0 0 0.05 0 0.11 0.06 0.1 0.15 0.16 0.11
Nucleus 0.05 0.09 0.07 0 0.05 0.06 0.1 0.08 0.09 0.1 0.09 0.11 0 0 0.06 0 0 0 0.09 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0.1 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.05 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0 0.07 0.08 0.2 0.06 0.08 0.07 0.05 0.05 0 0.08 0 0 0 0 0.11 0.21 0.2 0.21 0 0 0
Vac/Vac Membrane 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.14 0.05 0.06 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 7 2 7 0 3 2 1 2 1 1 2 0 0 0 1 0 2 4 2 6 4
Bud 0 0 3 5 15 7 13 15 18 17 22 13 3 1 0 0 0 10 14 23 4 6 5
Bud Neck 0 0 18 12 29 14 22 29 15 13 10 14 0 1 1 0 0 1 8 15 5 4 7
Bud Site 0 1 1 3 1 1 2 1 5 6 8 9 2 1 1 0 0
Cell Periphery 1 0 3 0 1 1 0 2 0 2 4 0 0 1 0 2 0 0 0 3 0 5 5
Cytoplasm 105 194 286 302 303 334 327 391 307 417 322 328 265 388 333 57 59 63 129 155 126 293 270
Endoplasmic Reticulum 3 4 8 1 3 4 1 0 0 0 0 0 0 0 2 4 5 1 0 2 1 8 2
Endosome 12 12 3 1 2 2 0 2 1 2 5 6 18 61 50 12 15 16 16 17 3 1 3
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 2 2 2 2 0 0 1 0
Mitochondria 8 34 1 13 10 58 91 47 169 212 206 137 21 10 16 5 4 22 18 42 28 67 38
Nucleus 8 25 26 14 24 27 51 44 45 64 54 64 9 10 25 3 2 7 28 15 1 0 2
Nuclear Periphery 3 2 3 6 1 8 6 3 10 11 5 5 4 6 2 0 1 10 10 14 3 2 1
Nucleolus 17 5 3 6 13 9 9 2 16 16 31 17 17 27 21 3 6 2 4 3 1 0 0
Peroxisomes 1 0 0 0 0 0 0 0 1 1 0 2 2 1 4 0 0 1 0 1 0 0 0
SpindlePole 15 11 26 30 90 26 37 38 27 33 23 48 15 12 13 2 11 42 64 90 5 4 2
Vac/Vac Membrane 15 29 13 7 11 10 5 17 5 8 14 16 7 14 14 22 14 10 18 24 8 16 10
Unique Cell Count 163 293 382 370 456 452 493 535 502 664 583 570 340 493 453 98 98 199 328 434 196 433 358
Labelled Cell Count 189 318 401 403 510 501 567 593 620 804 705 660 365 534 482 112 120 199 328 434 196 433 358


Bud Neck, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.9 4.7 4.3 4.9 3.7 3.8 4.2 3.4 3.3 3.3 3.8 3.9 4.0 4.2 6.4 6.2 3.7 3.5 3.8
Std Deviation (1e-4) 1.0 1.0 0.9 1.1 1.2 1.0 1.0 1.1 1.1 0.9 1.0 1.0 1.0 1.2 1.5 2.0 1.7 1.2 1.3 0.9
Intensity Change (Log2) -0.15 0.06 -0.36 -0.3 -0.19 -0.49 -0.54 -0.5 -0.31 -0.27 -0.24 -0.17 0.43 0.4 -0.37 -0.42 -0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 6.5 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.569 -0.2468 0.0053 1.3495 1.0737 -0.4586 -0.6765 -0.3299 0.0187 0.9773 1.1029 -0.5358 -0.869 0.1332 0.0899 1.1084 1.7813 -0.4957
Actin 0.012 0.0008 0.0055 0.0134 0.0054 0.0018 0.0386 0.0047 0.0157 0.0017 0.004 0.0066 0.0278 0.0018 0.0213 0.0386 0.0114 0.0052
Bud 0.0015 0.0037 0.0021 0.0024 0.0006 0.0009 0.0021 0.0034 0.0009 0.0002 0.0004 0.0015 0.0033 0.0041 0.0005 0.0005 0.0007 0.0009
Bud Neck 0.017 0.0069 0.0058 0.0272 0.0693 0.0826 0.0293 0.0015 0.0098 0.0163 0.0898 0.1015 0.006 0.0007 0.0013 0.0019 0.1315 0.1484
Bud Periphery 0.0039 0.0037 0.0024 0.0027 0.0017 0.0012 0.0027 0.0034 0.0014 0.0009 0.0013 0.0031 0.0076 0.0043 0.0009 0.001 0.0023 0.0025
Bud Site 0.0067 0.0168 0.0209 0.0091 0.0018 0.0019 0.033 0.029 0.0049 0.0003 0.0016 0.0004 0.0087 0.0078 0.0161 0.0022 0.0023 0.0014
Cell Periphery 0.0006 0.0004 0.0004 0.0003 0.0003 0.0002 0.0007 0.0004 0.0002 0.0001 0.0003 0.0002 0.0006 0.0003 0.0002 0.0002 0.0006 0.0003
Cytoplasm 0.0662 0.0744 0.0365 0.0401 0.0444 0.0974 0.0819 0.09 0.0241 0.008 0.0325 0.2011 0.0501 0.1141 0.0355 0.0047 0.0081 0.1327
Cytoplasmic Foci 0.0644 0.0191 0.0504 0.007 0.0127 0.0329 0.0823 0.0452 0.1012 0.0021 0.0206 0.0266 0.1112 0.0353 0.062 0.0039 0.0216 0.0328
Eisosomes 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0006 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0096 0.0057 0.0036 0.0047 0.0049 0.0047 0.0063 0.0026 0.0029 0.0029 0.0075 0.0048 0.0075 0.0045 0.0022 0.0032 0.0057 0.0032
Endosome 0.0296 0.0064 0.0177 0.0113 0.018 0.0098 0.0282 0.0047 0.0283 0.0051 0.0666 0.0096 0.0403 0.0143 0.0149 0.0064 0.0426 0.012
Golgi 0.0028 0.0004 0.0042 0.0044 0.0068 0.0011 0.0145 0.0012 0.0121 0.0002 0.0067 0.001 0.0065 0.0075 0.0066 0.0059 0.0095 0.0024
Lipid Particles 0.0198 0.0035 0.0163 0.0048 0.0399 0.0059 0.05 0.0056 0.0239 0.0025 0.0229 0.0046 0.0552 0.0059 0.0287 0.0173 0.052 0.0077
Mitochondria 0.0061 0.0013 0.0035 0.002 0.012 0.0015 0.0095 0.0035 0.0165 0.0018 0.0036 0.0013 0.0131 0.0031 0.0043 0.0049 0.0182 0.0013
None 0.556 0.548 0.2642 0.008 0.0427 0.5885 0.4386 0.499 0.1357 0.0049 0.0049 0.5109 0.4173 0.4429 0.211 0.0026 0.0034 0.5522
Nuclear Periphery 0.0686 0.0609 0.1409 0.2659 0.2529 0.0305 0.047 0.0762 0.1182 0.3741 0.2981 0.0329 0.0692 0.0744 0.1526 0.2888 0.367 0.0249
Nucleolus 0.0157 0.0088 0.0185 0.0106 0.0295 0.0081 0.0071 0.0066 0.0086 0.0108 0.0253 0.003 0.0078 0.0066 0.0099 0.0087 0.037 0.0031
Nucleus 0.0545 0.1573 0.1886 0.4433 0.2816 0.0619 0.0229 0.136 0.1246 0.5045 0.2497 0.0378 0.0233 0.1329 0.1418 0.4609 0.128 0.028
Peroxisomes 0.0067 0.0015 0.003 0.0014 0.0249 0.0012 0.025 0.0029 0.0154 0.0002 0.0027 0.0032 0.0326 0.0011 0.0087 0.0083 0.0206 0.0028
Punctate Nuclear 0.034 0.0723 0.2072 0.1359 0.1374 0.0617 0.0695 0.0785 0.3486 0.0502 0.144 0.0418 0.0939 0.1321 0.2756 0.1331 0.1155 0.0338
Vacuole 0.0159 0.006 0.0043 0.0024 0.0053 0.0046 0.0068 0.0035 0.0035 0.0041 0.009 0.0056 0.0111 0.0039 0.0023 0.0017 0.008 0.0029
Vacuole Periphery 0.0081 0.002 0.0039 0.003 0.0077 0.0018 0.0035 0.002 0.0033 0.0092 0.0083 0.0024 0.0065 0.0024 0.0034 0.0052 0.0138 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.075 53.0911 50.4544 40.0409 19.5882 8.8515 57.5651 72.9379 44.9577 22.6008
Translational Efficiency 1.0927 0.9682 0.9339 0.7645 0.6557 0.8499 0.909 0.693 0.8939 0.7558

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency