Standard name
Description Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.98 0.95 0.96 – 0.81 0.8 0.86 0.76 0.76 0.99 0.99 0.99 0.96 0.99 – 0.94 0.95 0.9 0.91 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.08 0.07 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0.12 0.13 0 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 – 0.08 0.06 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 3 8 4 2 2 0 0 0 0 0 – 0 0 0 0 0 0
Bud 1 0 0 0 0 0 – 0 0 0 2 0 0 2 1 0 0 – 3 0 1 1 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 2 0 0 2 1 4 – 5 4 2 5 12 0 1 1 1 0 – 0 0 0 0 0 1
Cytoplasm 482 163 71 135 140 216 – 198 140 190 139 155 338 354 383 110 113 – 455 163 79 172 215 250
Endoplasmic Reticulum 0 0 0 1 1 2 – 20 13 8 10 9 1 4 2 5 1 – 1 0 0 1 0 3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 – 3 0 2 0 0 1 0 0 0 0 – 0 0 0 0 0 1
Mitochondria 0 0 0 1 0 3 – 5 5 7 23 27 0 0 0 0 1 – 0 0 1 0 0 2
Nucleus 0 0 0 0 0 0 – 1 3 7 5 5 2 0 1 1 0 – 9 3 1 5 3 2
Nuclear Periphery 0 0 1 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 1 0 2 0 1 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 4 0 1 1 7 2 – 19 10 8 5 5 0 1 0 4 2 – 5 2 1 2 4 3
Unique Cell Count 487 163 73 138 148 224 244 174 221 184 203 341 359 385 114 114 484 172 88 189 229 274
Labelled Cell Count 489 163 73 140 149 227 254 184 228 194 215 343 362 388 121 117 484 172 88 189 229 274


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.7 42.5 25.1 35.2 36.5 30.0 – 24.1 21.3 20.8 18.9 19.4 42.6 43.8 41.9 64.4 64.5 – 33.7 38.3 38.0
Std Deviation (1e-4) 8.4 7.8 9.0 9.8 10.4 7.4 – 5.8 5.2 5.4 4.9 5.1 9.7 10.2 9.6 26.7 24.7 – 9.3 9.5 9.7
Intensity Change (Log2) – – – 0.49 0.54 0.26 – -0.05 -0.24 -0.27 -0.41 -0.37 0.77 0.81 0.74 1.36 1.36 – 0.43 0.61 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0.3 -0.9 -0.3 – -3.4 -3.4 -2.6 -4.1 -4.0 1.3 0.8 1.9 -0.3 1.0 –
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 0 – 0 0 0 3.2 3.3 0 0 0 0 0 –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –