Standard name | |
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Description | Protein involved in phospholipid flippase function; null allele suppresses growth and membrane trafficking defects associated with all flippase null alleles; proposed function as a phospholipid scramblase that reduces membrane asymmetry; PQ loop family member; localizes to the endosome and trans-Golgi network; non-essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 7 | 7 | 3 | 3 | 6 | 8 | 9 | 13 | 1 | 8 | 8 | 3 | 2 | 20 | 20 | 3 | 2 | 1 | 9 | 0 | 1 | 4 |
Bud | 0 | 1 | 4 | 2 | 0 | 6 | 6 | 8 | 17 | 26 | 24 | 26 | 2 | 0 | 1 | 2 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 6 | 3 | 9 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 10 | 12 | 28 | 44 | 52 | 60 | 51 | 56 | 40 | 57 | 1 | 13 | 5 | 30 | 28 | 15 | 0 | 0 | 0 | 0 | 1 | 2 |
Endoplasmic Reticulum | 2 | 4 | 3 | 1 | 1 | 2 | 6 | 4 | 8 | 4 | 3 | 6 | 5 | 3 | 4 | 16 | 8 | 3 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 3 | 1 | 37 | 31 | 60 | 45 | 53 | 86 | 46 | 57 | 57 | 78 | 5 | 7 | 20 | 19 | 19 | 12 | 11 | 17 | 27 | 19 | 24 | 20 |
Golgi | 29 | 42 | 100 | 58 | 54 | 19 | 27 | 29 | 47 | 23 | 12 | 15 | 52 | 78 | 117 | 133 | 82 | 39 | 74 | 69 | 158 | 70 | 107 | 149 |
Mitochondria | 67 | 30 | 106 | 92 | 55 | 70 | 111 | 56 | 98 | 132 | 119 | 111 | 28 | 20 | 24 | 19 | 13 | 7 | 2 | 3 | 7 | 6 | 8 | 4 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 6 | 3 | 8 | 9 | 6 | 16 | 17 | 11 | 9 | 12 | 8 | 7 | 4 | 2 | 1 | 7 | 14 | 3 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 5 | 0 | 1 | 4 | 10 | 4 | 6 | 4 | 8 | 1 | 1 | 4 | 1 | 7 | 3 | 0 | 0 | 1 | 1 | 0 | 0 |
Vac/Vac Membrane | 14 | 20 | 21 | 19 | 70 | 50 | 81 | 68 | 57 | 52 | 51 | 51 | 12 | 13 | 16 | 94 | 70 | 61 | 4 | 10 | 2 | 6 | 7 | 7 |
Unique Cell Count | 81 | 72 | 199 | 160 | 210 | 207 | 287 | 266 | 265 | 300 | 251 | 302 | 77 | 109 | 150 | 230 | 185 | 110 | 99 | 108 | 222 | 111 | 155 | 196 |
Labelled Cell Count | 123 | 111 | 302 | 238 | 277 | 257 | 369 | 346 | 351 | 391 | 327 | 382 | 118 | 142 | 196 | 342 | 270 | 148 | 99 | 108 | 222 | 111 | 155 | 196 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 13.6 | 11.6 | 10.2 | 11.2 | 9.7 | 9.5 | 9.9 | 9.1 | 9.1 | 8.5 | 8.6 | 11.6 | 12.1 | 11.2 | 15.0 | 13.8 | 14.3 | 12.8 | 12.9 | 13.8 |
Std Deviation (1e-4) | 1.4 | 2.6 | 1.7 | 1.7 | 1.9 | 1.9 | 1.8 | 2.1 | 1.6 | 1.7 | 1.5 | 1.6 | 2.2 | 2.2 | 1.8 | 3.1 | 3.0 | 3.2 | 2.1 | 2.0 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.05 | -0.26 | -0.29 | -0.24 | -0.35 | -0.36 | -0.45 | -0.43 | 0.0 | 0.06 | -0.06 | 0.37 | 0.25 | 0.3 | 0.14 | 0.15 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | 0 | 0 | -1.0 | -0.3 | -0.1 | 0.5 | 0 | -0.6 | 0 | 0 | 0 | 2.2 | 2.8 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.7 | 2.3 | 3.1 | 3.3 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 2.9 | 4.8 | 4.3 | 5.2 | 4.5 | 4.4 | 3.7 | 4.5 | 0 | 2.2 | -0.8 | 2.8 | 3.3 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 1.7 | 0 |
Endosome | 0.2 | 2.4 | 0.8 | 0 | 3.3 | -0.3 | 0.1 | 1.1 | 1.9 | -2.5 | -2.9 | -1.3 | -3.2 | -2.3 | -1.8 |
Golgi | -2.7 | -5.1 | -9.1 | -10.1 | -9.4 | -7.5 | -10.8 | -11.1 | -11.8 | 2.6 | 3.6 | 5.3 | 1.6 | -1.2 | -2.5 |
Mitochondria | 0.8 | -5.6 | -4.0 | -3.2 | -7.2 | -3.5 | -2.0 | -1.2 | -3.6 | -2.5 | -6.0 | -7.1 | -10.2 | -9.8 | -8.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.7 | -0.6 | 1.6 | 0.9 | 0.1 | -0.4 | 0 | -0.5 | -1.1 | 0 | 0 | 0 | -0.5 | 1.5 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | 5.5 | 3.6 | 4.7 | 4.1 | 3.1 | 2.1 | 2.8 | 2.0 | 1.2 | 0.4 | 0 | 7.1 | 6.3 | 8.6 |