Standard name
Description Protein involved in phospholipid flippase function; null allele suppresses growth and membrane trafficking defects associated with all flippase null alleles; proposed function as a phospholipid scramblase that reduces membrane asymmetry; PQ loop family member; localizes to the endosome and trans-Golgi network; non-essential gene
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0.09 0.11 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0.09 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.08 0.13 0.21 0.18 0.23 0.19 0.19 0.16 0.19 0 0.12 0 0.13 0.15 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0.19 0.19 0.29 0.22 0.18 0.32 0.17 0.19 0.23 0.26 0.06 0.06 0.13 0.08 0.1 0.11 0.11 0.17 0.13 0.18 0.16 0.1
Golgi 0.36 0.58 0.5 0.36 0.26 0.09 0.09 0.11 0.18 0.08 0.05 0.05 0.68 0.72 0.78 0.58 0.44 0.35 0.76 0.64 0.72 0.63 0.69 0.76
Mitochondria 0.83 0.42 0.53 0.57 0.26 0.34 0.39 0.21 0.37 0.44 0.47 0.37 0.36 0.18 0.16 0.08 0.07 0.06 0 0 0 0.06 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0.06 0 0.08 0.06 0 0 0 0 0 0.05 0 0 0 0.08 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.28 0.11 0.12 0.33 0.24 0.28 0.26 0.22 0.17 0.2 0.17 0.16 0.12 0.11 0.41 0.38 0.55 0 0.1 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 7 7 3 3 6 8 9 13 1 8 8 3 2 20 20 3 2 1 9 0 1 4
Bud 0 1 4 2 0 6 6 8 17 26 24 26 2 0 1 2 4 1 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 1 0 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 2 1 3 6 3 9 0 0 1 0 3 1 0 0 0 0 0 0
Cytoplasm 1 3 10 12 28 44 52 60 51 56 40 57 1 13 5 30 28 15 0 0 0 0 1 2
Endoplasmic Reticulum 2 4 3 1 1 2 6 4 8 4 3 6 5 3 4 16 8 3 0 0 1 0 1 1
Endosome 3 1 37 31 60 45 53 86 46 57 57 78 5 7 20 19 19 12 11 17 27 19 24 20
Golgi 29 42 100 58 54 19 27 29 47 23 12 15 52 78 117 133 82 39 74 69 158 70 107 149
Mitochondria 67 30 106 92 55 70 111 56 98 132 119 111 28 20 24 19 13 7 2 3 7 6 8 4
Nucleus 0 0 1 1 0 0 2 1 0 0 1 2 0 2 0 0 0 0 0 1 0 0 0 1
Nuclear Periphery 1 1 1 0 0 1 1 0 1 0 2 1 0 0 1 1 2 0 0 0 0 0 0 0
Nucleolus 6 3 8 9 6 16 17 11 9 12 8 7 4 2 1 7 14 3 0 0 0 1 1 0
Peroxisomes 0 0 1 1 0 0 0 3 0 4 1 0 0 0 0 0 0 0 1 0 8 0 0 1
SpindlePole 0 0 2 5 0 1 4 10 4 6 4 8 1 1 4 1 7 3 0 0 1 1 0 0
Vac/Vac Membrane 14 20 21 19 70 50 81 68 57 52 51 51 12 13 16 94 70 61 4 10 2 6 7 7
Unique Cell Count 81 72 199 160 210 207 287 266 265 300 251 302 77 109 150 230 185 110 99 108 222 111 155 196
Labelled Cell Count 123 111 302 238 277 257 369 346 351 391 327 382 118 142 196 342 270 148 99 108 222 111 155 196


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 13.6 11.6 10.2 11.2 9.7 9.5 9.9 9.1 9.1 8.5 8.6 11.6 12.1 11.2 15.0 13.8 14.3 12.8 12.9 13.8
Std Deviation (1e-4) 1.4 2.6 1.7 1.7 1.9 1.9 1.8 2.1 1.6 1.7 1.5 1.6 2.2 2.2 1.8 3.1 3.0 3.2 2.1 2.0 2.3
Intensity Change (Log2) – – – -0.19 -0.05 -0.26 -0.29 -0.24 -0.35 -0.36 -0.45 -0.43 0.0 0.06 -0.06 0.37 0.25 0.3 0.14 0.15 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 0 0 -1.0 -0.3 -0.1 0.5 0 -0.6 0 0 0 2.2 2.8 0
Bud 0 0 0 0 0.7 2.3 3.1 3.3 3.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 2.9 4.8 4.3 5.2 4.5 4.4 3.7 4.5 0 2.2 -0.8 2.8 3.3 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.7 1.7 0
Endosome 0.2 2.4 0.8 0 3.3 -0.3 0.1 1.1 1.9 -2.5 -2.9 -1.3 -3.2 -2.3 -1.8
Golgi -2.7 -5.1 -9.1 -10.1 -9.4 -7.5 -10.8 -11.1 -11.8 2.6 3.6 5.3 1.6 -1.2 -2.5
Mitochondria 0.8 -5.6 -4.0 -3.2 -7.2 -3.5 -2.0 -1.2 -3.6 -2.5 -6.0 -7.1 -10.2 -9.8 -8.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.7 -0.6 1.6 0.9 0.1 -0.4 0 -0.5 -1.1 0 0 0 -0.5 1.5 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 5.5 3.6 4.7 4.1 3.1 2.1 2.8 2.0 1.2 0.4 0 7.1 6.3 8.6