Standard name
Human Ortholog
Description Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant; eIF3 is also involved in programmed stop codon readthrough
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0.07 0.05 0.06 – 0.05 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0.08 0 – 0.16 – – 0 0.05 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.9 – 0.81 0.75 0.71 0.59 – 0.58 – – 0.97 0.95 0.96 0.91 0.87 0.96 0.92 0.93 0.8 0.86 0.85 0.81
Endoplasmic Reticulum 0.06 0 0 – 0.05 0.11 0.16 0.12 – 0.16 – – 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0.1
Endosome 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0.05 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0.05 0.11 0.08 0.17 – 0.26 – – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 – 0.11 0 0.05 0.09 – 0 – – 0 0 0 0 0.05 0 0 0 0.06 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 – 1 2 2 4 – 1 – – 0 0 2 1 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 1 0 1 – 0 – – 0 0 0 0 1 0 4 1 0 0 1 1
Bud Neck 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 7 0 – 0 1 3 2 – 3 – – 1 11 7 8 6 3 0 0 0 0 0 1
Cytoplasm 409 219 57 – 30 21 27 41 – 11 – – 169 213 218 227 93 121 415 219 68 100 252 230
Endoplasmic Reticulum 26 8 2 – 2 3 6 8 – 3 – – 4 4 3 10 3 4 9 1 6 6 21 28
Endosome 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 1 1 0 1 0 0 0 2 0
Golgi 2 0 0 – 0 0 2 3 – 0 – – 0 0 0 10 3 3 0 0 0 0 1 6
Mitochondria 0 1 1 – 2 3 3 12 – 5 – – 1 0 2 2 0 1 1 0 3 0 1 0
Nucleus 1 0 0 – 0 0 0 0 – 0 – – 0 1 0 0 0 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 – 0 0 1 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 – 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 2 2 – 4 0 2 6 – 0 – – 1 0 1 10 5 3 7 8 5 5 9 9
Unique Cell Count 436 227 63 37 28 38 69 19 174 224 228 250 107 126 450 235 85 117 295 284
Labelled Cell Count 452 237 64 39 31 46 77 23 176 229 233 269 112 135 450 235 85 117 295 284


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.6 41.8 20.3 – 16.3 19.3 19.3 14.1 – 11.8 – – 40.3 37.3 32.0 36.9 39.7 39.9 40.2 45.0 47.0
Std Deviation (1e-4) 10.6 9.8 4.5 – 5.7 3.7 4.4 5.1 – 3.7 – – 7.5 7.9 6.7 12.1 12.6 13.9 8.6 11.0 12.4
Intensity Change (Log2) – – – – -0.32 -0.07 -0.08 -0.53 – -0.78 – – 0.99 0.88 0.65 0.86 0.97 0.97 0.98 1.14 1.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Bud – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Cell Periphery – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Cytoplasm – -1.3 -1.9 -2.5 -4.1 – -3.3 – – 2.1 1.4 1.6 0.1 -0.7 1.5
Endoplasmic Reticulum – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Endosome – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Golgi – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Mitochondria – 0 0 0 3.0 – 0 – – 0 0 0 0 0 0
Nucleus – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 – 0 – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 – 0 – – 0 0 0 0 0 0
Vacuole – 0 0 0 0 – 0 – – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 31.3447 44.9511 38.6422 35.9928 36.2822 37.1838 31.9282 47.5807 42.0363 40.0103 37.67 43.949 35.0319 50.7849 43.8175 44.7134 41.192 44.4928
Actin 0.0041 0.0017 0.0065 0.0118 0.0055 0.0077 0.0153 0.0018 0.0074 0.0091 0.0317 0.0019 0.0142 0.0006 0.0012 0.0015 0.0014 0.0024
Bud 0.002 0.0025 0.0028 0.0087 0.0042 0.002 0.0017 0.0006 0.0013 0.0039 0.0014 0.0009 0.0022 0.0004 0.0014 0.0017 0.0011 0.0008
Bud Neck 0.0011 0.0015 0.0025 0.002 0.0059 0.0028 0.0082 0.0004 0.0008 0.0011 0.0009 0.0018 0.0006 0.0003 0.0006 0.0008 0.0005 0.0011
Bud Periphery 0.0016 0.0016 0.0018 0.0044 0.0068 0.0016 0.0014 0.0004 0.0013 0.0039 0.0014 0.0003 0.0021 0.0001 0.0005 0.0003 0.0004 0.0005
Bud Site 0.0011 0.0025 0.0026 0.0016 0.001 0.0004 0.0099 0.0005 0.0012 0.0136 0.0006 0.0001 0.0015 0.0002 0.0003 0.0003 0.0002 0.0001
Cell Periphery 0.0037 0.0098 0.0084 0.0022 0.0081 0.0017 0.0011 0.0016 0.0013 0.0033 0.0003 0.0003 0.0045 0.0003 0.0002 0.0001 0.0001 0.0003
Cytoplasm 0.5968 0.8157 0.7015 0.527 0.4922 0.5772 0.7613 0.9529 0.8797 0.7162 0.7864 0.803 0.6565 0.9419 0.8558 0.8083 0.7513 0.7205
Cytoplasmic Foci 0.0056 0.006 0.0078 0.0118 0.0091 0.0132 0.0147 0.0051 0.0101 0.0156 0.0125 0.0205 0.0388 0.0072 0.0133 0.02 0.0301 0.0225
Eisosomes 0 0 0 0 0 0.0001 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0797 0.0303 0.0292 0.0371 0.0082 0.065 0.0402 0.0059 0.0081 0.0082 0.0199 0.036 0.0215 0.0058 0.0084 0.0123 0.0067 0.0469
Endosome 0.0476 0.0274 0.0395 0.064 0.114 0.0958 0.0461 0.0099 0.0317 0.0847 0.0527 0.0928 0.0886 0.0201 0.0596 0.0929 0.1237 0.1253
Golgi 0.0065 0.0044 0.0107 0.0119 0.0234 0.0333 0.0064 0.0037 0.0096 0.01 0.0167 0.0074 0.0233 0.0043 0.0097 0.0125 0.0125 0.0319
Lipid Particles 0.0009 0.0007 0.0016 0.0009 0.0005 0.0003 0.0021 0.0003 0.0014 0.0009 0.0009 0.0002 0.0098 0.0002 0.0038 0.0001 0.0005 0.0001
Mitochondria 0.0046 0.0038 0.0037 0.0179 0.0045 0.0246 0.0178 0.0024 0.0099 0.0193 0.009 0.0023 0.0194 0.0107 0.0107 0.0035 0.0061 0.0153
None 0.0008 0.0003 0.0006 0.0008 0.0004 0.0007 0.0047 0.0006 0.0018 0.0006 0.0007 0.0003 0.0034 0.0002 0.0009 0.0003 0.0021 0.0004
Nuclear Periphery 0.0503 0.005 0.0109 0.0259 0.0226 0.0211 0.0181 0.001 0.0039 0.0035 0.0142 0.0036 0.015 0.0011 0.002 0.002 0.0049 0.0035
Nucleolus 0.0002 0.0001 0.0003 0.0006 0.0005 0.0001 0.0005 0.0001 0.0009 0.0001 0.0005 0 0.0006 0 0.0004 0 0.0004 0
Nucleus 0.0096 0.0026 0.0064 0.0112 0.0048 0.0039 0.0066 0.0009 0.002 0.0018 0.0037 0.0011 0.0047 0.0005 0.001 0.0009 0.0036 0.0008
Peroxisomes 0.0001 0.0001 0.0002 0.0014 0.0002 0.0002 0.0004 0.0001 0.0006 0.0007 0.0004 0.0002 0.0022 0 0.0013 0.0003 0.0003 0.0002
Punctate Nuclear 0.0002 0.0001 0.0002 0.0009 0.0001 0.0001 0.0088 0.0001 0.0028 0.0004 0.0007 0.0001 0.0033 0 0.0002 0.0001 0.0013 0
Vacuole 0.1192 0.065 0.1261 0.181 0.1941 0.0719 0.026 0.0103 0.0181 0.0711 0.0275 0.0138 0.0626 0.0042 0.0181 0.0259 0.0337 0.0134
Vacuole Periphery 0.0644 0.0188 0.0368 0.0768 0.094 0.0766 0.0084 0.0015 0.006 0.0318 0.0176 0.0132 0.0252 0.0018 0.0106 0.0162 0.0188 0.014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 241.1719 201.1858 182.888 216.2579 192.7259 175.7451 198.5413 188.1208 177.7911 208.287
Translational Efficiency 0.9175 1.0674 1.0358 0.8566 0.884 1.1821 0.9087 0.7695 0.8388 0.826

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2081 168 344 1205 2211 1681 918 204 4292 1849 1262 1409

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2371.60 3100.70 4734.67 2572.02 2638.03 2722.46 3574.20 3032.62 2508.85 2756.83 3890.52 2638.71
Standard Deviation 398.08 546.31 717.63 621.87 448.63 525.58 718.76 733.01 445.25 538.58 884.98 659.39
Intensity Change Log 2 — 0.386733 0.997403 0.117041 — 0.045450 0.438159 0.201104 — 0.217098 0.729948 0.161918

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000018 0.000193 0.000102 0.000244 0.000037 0.000060 0.000098 0.000279 0.000028 0.000072 0.000099 0.000249
Bud Neck 0.000038 0.001012 0.004882 0.001904 0.000121 0.000182 0.002989 0.002369 0.000081 0.000257 0.003505 0.001971
Bud Site 0.003812 0.013820 0.004178 0.016891 0.005575 0.005307 0.005576 0.014749 0.004720 0.006081 0.005195 0.016581
Cell Periphery 0.001377 0.008571 0.003849 0.001455 0.001859 0.001002 0.003135 0.006665 0.001625 0.001690 0.003330 0.002210
Cytoplasm 0.627933* 0.311708* 0.225546* 0.234871* 0.495601* 0.563534* 0.230944* 0.136779* 0.559762* 0.540653* 0.229473* 0.220668*
Cytoplasmic Foci 0.061203 0.052943 0.029702 0.015883 0.093514 0.054177 0.017704 0.017577 0.077848 0.054065 0.020974 0.016128
Eisosomes 0.000001 0.000003 0.000003 0.000005 0.000001 0.000001 0.000002 0.000006 0.000001 0.000001 0.000003 0.000005
Endoplasmic Reticulum 0.002861 0.002333 0.000284 0.000609 0.002783 0.000755 0.000465 0.000747 0.002821 0.000898 0.000416 0.000629
Endosome 0.001251 0.006931 0.002624 0.011391 0.003460 0.003066 0.004639 0.011058 0.002389 0.003417 0.004090 0.011343
Golgi 0.068785 0.349600* 0.137949* 0.057194 0.192195* 0.142235* 0.114663* 0.115470* 0.132359* 0.161076* 0.121010* 0.065631
Lipid Particles 0.000588 0.007536 0.007469 0.005312 0.002576 0.001035 0.004746 0.014924 0.001612 0.001626 0.005488 0.006703
Mitochondria 0.001203 0.000125 0.000186 0.001695 0.001435 0.000843 0.000299 0.002722 0.001322 0.000778 0.000268 0.001844
Mitotic Spindle 0.001463 0.005347 0.008466 0.009862 0.002011 0.003065 0.005080 0.010034 0.001745 0.003272 0.006003 0.009887
None 0.001937 0.000707 0.000936 0.002222 0.000840 0.000768 0.000903 0.000459 0.001372 0.000762 0.000912 0.001967
Nuclear Periphery 0.000109 0.000509 0.004703 0.000724 0.000170 0.000117 0.002912 0.000515 0.000140 0.000152 0.003400 0.000693
Nuclear Periphery Foci 0.002458 0.010238 0.028419 0.010516 0.004789 0.004319 0.028513 0.012323 0.003658 0.004856 0.028487 0.010778
Nucleolus 0.000027 0.000064 0.000269 0.000646 0.000029 0.000044 0.000344 0.001101 0.000028 0.000046 0.000323 0.000712
Nucleus 0.000636 0.000831 0.008364 0.004094 0.000523 0.000932 0.008485 0.003190 0.000578 0.000923 0.008452 0.003963
Peroxisomes 0.000022 0.000056 0.000024 0.000453 0.000033 0.000041 0.000025 0.000260 0.000028 0.000043 0.000025 0.000425
Vacuole 0.015020 0.064087 0.295241* 0.452621* 0.030448 0.047217 0.322738* 0.485608* 0.022968 0.048750 0.315243* 0.457397*
Vacuole Periphery 0.209261* 0.163385* 0.236802* 0.171409* 0.161999* 0.171302* 0.245742* 0.163165* 0.184915* 0.170582* 0.243305* 0.170215*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.25 -7.32 -3.77 -0.65 -1.94 -1.60 -6.84 -2.91 -2.62 -1.99 -3.24 -9.76 -4.25 -3.27 -2.42
Bud Neck -4.05 -9.04 -11.06 -4.27 5.17 -1.99 -11.16 -5.81 -5.67 1.28 -5.00 -14.35 -12.27 -11.16 5.48
Bud Site -3.45 -0.98 -7.10 -0.74 -6.40 0.26 -1.14 -3.96 -4.02 -3.64 -2.75 -2.15 -7.54 -6.44 -6.61
Cell Periphery -4.13 -8.70 0.34 4.15 8.32 6.00 -2.22 -4.40 -5.29 -2.83 -0.35 -4.31 -1.90 -1.24 3.02
Cytoplasm 11.88 22.79 33.35 2.03 -1.69 -6.10 21.17 19.01 22.22 4.47 2.03 33.14 34.05 26.59 -0.52
Cytoplasmic Foci 1.51 7.52 18.98 7.25 5.77 12.28 26.35 23.28 13.12 -0.06 9.60 26.30 32.02 19.08 4.47
Eisosomes -4.02 -7.33 -5.36 -1.46 -0.99 3.74 -4.69 -2.34 -2.57 -1.69 -0.59 -8.38 -5.46 -5.31 -2.51
Endoplasmic Reticulum 0.37 10.74 9.48 1.23 -4.17 5.95 6.84 5.38 0.18 -1.28 7.66 11.47 10.45 2.05 -2.80
Endosome -8.15 -7.42 -10.03 -3.31 -8.29 1.90 -4.88 -3.30 -3.46 -2.56 -6.22 -8.46 -9.60 -8.31 -7.05
Golgi -14.23 -8.65 4.93 15.17 11.18 7.92 13.91 8.39 3.58 0.16 -5.30 4.11 19.07 19.42 12.59
Lipid Particles -5.40 -11.12 -5.58 0.95 1.86 6.60 -6.48 -5.09 -5.73 -3.98 -0.11 -13.09 -6.26 -6.20 -1.32
Mitochondria 13.11 12.07 -0.44 -2.24 -2.10 2.07 3.86 -0.64 -1.03 -1.35 3.17 6.43 -0.50 -1.47 -2.31
Mitotic Spindle -3.60 -5.64 -5.09 -2.32 -0.23 -2.69 -5.96 -2.37 -2.09 -1.36 -4.33 -8.43 -5.47 -4.33 -1.94
None 10.91 7.95 -0.09 -1.93 -1.52 1.09 -0.51 2.54 2.01 2.14 9.95 3.59 -0.72 -1.79 -1.46
Nuclear Periphery -1.50 -7.98 -5.77 -0.80 6.84 1.83 -5.17 -3.28 -3.67 4.20 -0.50 -7.82 -6.07 -5.77 6.35
Nuclear Periphery Foci -5.28 -13.38 -8.19 -0.56 7.95 0.85 -10.12 -4.11 -4.32 5.71 -3.04 -13.88 -8.06 -6.42 8.72
Nucleolus -2.73 -12.89 -3.39 -3.17 -1.85 -3.12 -7.66 -1.81 -1.78 -1.25 -4.20 -9.76 -3.84 -3.73 -1.94
Nucleus -1.24 -6.64 -7.30 -6.86 3.29 -3.74 -6.57 -2.22 -2.06 1.73 -3.48 -8.30 -7.34 -6.75 4.10
Peroxisomes -2.88 -2.06 -2.16 -1.96 -2.12 -0.77 1.29 -2.52 -2.40 -2.58 -1.55 -0.30 -2.33 -2.22 -2.33
Vacuole -7.29 -21.88 -55.25 -37.54 -9.79 -7.17 -36.81 -22.45 -21.51 -7.01 -12.40 -43.39 -58.78 -53.57 -13.48
Vacuole Periphery 3.55 -0.85 8.35 1.07 5.66 -1.47 -7.95 1.19 1.90 5.84 2.65 -5.77 6.41 3.32 9.98
Standard name
Human Ortholog
Description Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant; eIF3 is also involved in programmed stop codon readthrough
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Clu1

Clu1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Clu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available