Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 1 | 6 | 15 | 4 | – | 10 | 8 | 2 | 4 | 7 | 14 | 27 | 15 | 0 | 1 | 2 | 0 | 1 | 5 | 0 | 2 | 2 |
Bud | – | – | 5 | 12 | 20 | 18 | – | 35 | 46 | 39 | 33 | 38 | 0 | 4 | 3 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | – | 0 | 0 | 0 | 0 | – | 5 | 4 | 4 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 1 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | 32 | 21 | 31 | 13 | – | 15 | 8 | 4 | 6 | 6 | 5 | 46 | 94 | 43 | 55 | 58 | 1 | 2 | 0 | 0 | 3 | 2 |
Endoplasmic Reticulum | – | – | 12 | 3 | 10 | 4 | – | 2 | 3 | 2 | 1 | 2 | 19 | 26 | 17 | 3 | 5 | 5 | 0 | 0 | 1 | 2 | 3 | 7 |
Endosome | – | – | 128 | 125 | 182 | 145 | – | 135 | 24 | 38 | 16 | 42 | 12 | 40 | 19 | 3 | 39 | 36 | 15 | 13 | 159 | 32 | 75 | 60 |
Golgi | – | – | 43 | 44 | 71 | 43 | – | 22 | 9 | 8 | 4 | 6 | 5 | 9 | 2 | 0 | 8 | 9 | 18 | 1 | 150 | 21 | 56 | 37 |
Mitochondria | – | – | 96 | 70 | 81 | 275 | – | 364 | 347 | 545 | 323 | 433 | 8 | 13 | 4 | 3 | 16 | 14 | 9 | 10 | 20 | 12 | 26 | 23 |
Nucleus | – | – | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 3 | 0 | 1 | 1 | – | 3 | 2 | 5 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | – | – | 1 | 7 | 5 | 26 | – | 56 | 26 | 41 | 13 | 20 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 5 | 4 | 28 | 37 | – | 135 | 55 | 66 | 34 | 75 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 13 | 1 | 7 | 1 |
SpindlePole | – | – | 13 | 20 | 32 | 29 | – | 87 | 35 | 40 | 31 | 35 | 8 | 27 | 6 | 0 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | – | – | 87 | 22 | 20 | 16 | – | 10 | 5 | 5 | 3 | 7 | 4 | 10 | 12 | 25 | 69 | 66 | 19 | 13 | 39 | 10 | 52 | 122 |
Unique Cell Count | 323 | 241 | 369 | 480 | 631 | 430 | 645 | 381 | 522 | 62 | 178 | 161 | 63 | 158 | 142 | 71 | 46 | 394 | 85 | 233 | 265 | |||
Labelled Cell Count | 426 | 334 | 496 | 611 | 881 | 574 | 800 | 476 | 682 | 75 | 207 | 181 | 81 | 200 | 197 | 71 | 46 | 394 | 85 | 233 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 6.7 | 7.7 | 7.7 | 7.2 | – | 7.3 | 7.1 | 6.6 | 6.7 | 7.1 | 6.8 | 6.5 | 6.2 | 5.6 | 7.0 | 8.2 | 7.4 | 7.6 | 7.5 |
Std Deviation (1e-4) | – | – | 1.2 | 1.7 | 1.6 | 1.2 | – | 1.3 | 1.4 | 1.3 | 1.4 | 1.3 | 0.9 | 1.0 | 0.9 | 1.0 | 1.4 | 1.6 | 1.3 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.2 | 0.21 | 0.1 | – | 0.13 | 0.09 | -0.02 | 0.01 | 0.08 | 0.02 | -0.04 | -0.11 | -0.25 | 0.07 | 0.29 | 0.14 | 0.18 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 3.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 6.9 | 5.2 | 0 | 0 | 0 |
Bud | 2.4 | 2.7 | 1.8 | – | 2.9 | 4.9 | 3.2 | 4.2 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -0.7 | -4.3 | – | -5.1 | -4.9 | -7.2 | -4.9 | -6.0 | -0.5 | 4.7 | 11.5 | 10.7 | 6.7 | 7.8 |
Endoplasmic Reticulum | -1.8 | -0.8 | -2.9 | – | 0 | -2.9 | 0 | -3.4 | -3.7 | 0 | 4.4 | 3.0 | 0 | -0.3 | -0.1 |
Endosome | 2.9 | 2.6 | -2.8 | – | -6.0 | -11.5 | -13.1 | -11.6 | -11.1 | -3.0 | -3.9 | -6.3 | -5.3 | -3.2 | -3.0 |
Golgi | 1.6 | 2.1 | -2.0 | – | -5.7 | -6.0 | -7.9 | -6.5 | -7.4 | -1.1 | -2.9 | -4.3 | -3.1 | -2.8 | -2.2 |
Mitochondria | -0.2 | -2.3 | 7.7 | – | 8.2 | 14.1 | 17.0 | 14.8 | 15.5 | -2.7 | -5.8 | -7.0 | -4.1 | -4.8 | -4.6 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.9 | – | 5.3 | 4.2 | 4.4 | 2.9 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 3.7 | 3.8 | – | 8.2 | 5.6 | 4.9 | 4.3 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2.1 | 2.5 | 1.3 | – | 4.7 | 2.3 | 1.4 | 2.2 | 1.6 | 0 | 4.4 | -0.2 | 0 | -0.8 | 0 |
Vacuole | -5.3 | -7.8 | -9.8 | – | -12.3 | -10.7 | -13.1 | -10.4 | -11.5 | -3.5 | -5.8 | -5.0 | 2.0 | 3.7 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.2135 | 5.5351 | 4.782 | 5.3625 | 4.5849 | 4.9952 | 4.3538 | 6.594 | 5.7137 | 4.9707 | 5.2354 | 5.8213 | 4.9152 | 4.207 | 4.0103 | 5.9059 | 5.5306 | 4.4653 |
Actin | 0.0357 | 0.0083 | 0.0273 | 0.0006 | 0.0022 | 0.0045 | 0.0083 | 0.0027 | 0.0019 | 0.0008 | 0.0061 | 0.0111 | 0.0049 | 0.0028 | 0.0028 | 0.0038 | 0.0001 | 0.001 |
Bud | 0.0006 | 0.0045 | 0.0051 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Bud Neck | 0.0035 | 0.0004 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Bud Periphery | 0.0009 | 0.0015 | 0.0023 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0057 | 0.0108 | 0.0033 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 |
Cell Periphery | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0147 | 0.0046 | 0.0017 | 0.0018 | 0.001 | 0.0033 | 0.0018 | 0.0011 | 0.0008 | 0.0008 | 0.0008 | 0.0006 | 0.0014 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0003 |
Cytoplasmic Foci | 0.089 | 0.0974 | 0.0966 | 0.178 | 0.1219 | 0.086 | 0.0761 | 0.06 | 0.0553 | 0.0596 | 0.0835 | 0.0498 | 0.065 | 0.0884 | 0.0567 | 0.068 | 0.023 | 0.0503 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0008 | 0.0006 | 0.0005 | 0.0617 | 0.0019 | 0.0014 | 0.0004 | 0.0004 | 0.0008 | 0.0004 | 0.0004 | 0.0011 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0004 |
Endosome | 0.2738 | 0.312 | 0.2594 | 0.292 | 0.1274 | 0.2575 | 0.253 | 0.2834 | 0.2188 | 0.31 | 0.2825 | 0.1761 | 0.4453 | 0.1955 | 0.2513 | 0.3086 | 0.3032 | 0.1942 |
Golgi | 0.4558 | 0.496 | 0.5193 | 0.4755 | 0.588 | 0.5644 | 0.4974 | 0.6101 | 0.6784 | 0.5166 | 0.5407 | 0.6767 | 0.375 | 0.5868 | 0.5465 | 0.5359 | 0.6081 | 0.4877 |
Lipid Particles | 0.0362 | 0.0081 | 0.0137 | 0.0203 | 0.0391 | 0.0174 | 0.0284 | 0.004 | 0.0036 | 0.0077 | 0.0096 | 0.0143 | 0.0436 | 0.099 | 0.0063 | 0.0045 | 0.0125 | 0.2144 |
Mitochondria | 0.0254 | 0.0171 | 0.0386 | 0.0129 | 0.0366 | 0.0335 | 0.0386 | 0.0262 | 0.0293 | 0.0152 | 0.0547 | 0.0357 | 0.0346 | 0.0128 | 0.1162 | 0.0291 | 0.0061 | 0.0183 |
None | 0.0025 | 0.0003 | 0.0004 | 0.0016 | 0.0001 | 0.0002 | 0.0117 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0045 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0077 | 0.0146 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0006 | 0.0003 |
Nucleolus | 0.0002 | 0.0004 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0017 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.003 | 0.0005 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0357 | 0.0182 | 0.0192 | 0.0079 | 0.0185 | 0.011 | 0.027 | 0.004 | 0.007 | 0.005 | 0.0122 | 0.0276 | 0.0088 | 0.0075 | 0.0127 | 0.0428 | 0.0003 | 0.0207 |
Punctate Nuclear | 0.0022 | 0.0002 | 0.0009 | 0.0028 | 0.0009 | 0.0005 | 0.0071 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 |
Vacuole | 0.0037 | 0.0081 | 0.0037 | 0.0013 | 0.0005 | 0.003 | 0.0127 | 0.0025 | 0.0012 | 0.0602 | 0.0023 | 0.0018 | 0.0085 | 0.0015 | 0.0016 | 0.0014 | 0.0113 | 0.004 |
Vacuole Periphery | 0.0029 | 0.0105 | 0.0064 | 0.0021 | 0.0007 | 0.0052 | 0.0202 | 0.0042 | 0.0026 | 0.0217 | 0.0036 | 0.0052 | 0.0085 | 0.0037 | 0.0039 | 0.0038 | 0.0339 | 0.0078 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.3422 | 85.5015 | 72.6743 | 63.3533 | 58.6224 | 51.2725 | 80.9491 | 77.1788 | 65.2063 | 73.5477 |
Translational Efficiency | 0.7321 | 0.7659 | 0.6851 | 0.6702 | 0.7225 | 0.8854 | 0.7492 | 0.6019 | 0.6265 | 0.6263 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
40 | 1159 | 564 | 814 | 799 | 609 | 1705 | 924 | 839 | 1768 | 2269 | 1738 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 899.71 | 825.76 | 991.34 | 868.95 | 742.19 | 842.19 | 959.50 | 797.49 | 749.70 | 831.42 | 967.41 | 830.96 |
Standard Deviation | 104.37 | 135.04 | 109.59 | 141.77 | 100.54 | 120.88 | 145.52 | 152.62 | 106.17 | 130.57 | 138.16 | 151.88 |
Intensity Change Log 2 | — | -0.123738 | 0.139920 | -0.050187 | — | 0.182357 | 0.370494 | 0.103678 | — | 0.022710 | 0.248729 | 0.021403 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001001 | 0.003611 | 0.005391 | 0.008163 | 0.011812 | 0.000969 | 0.011941 | 0.006011 | 0.011296 | 0.002701 | 0.010313 | 0.007019 |
Bud Neck | 0.017780 | 0.000832 | 0.000986 | 0.007740 | 0.000591 | 0.001922 | 0.000270 | 0.006377 | 0.001411 | 0.001208 | 0.000448 | 0.007015 |
Bud Site | 0.010620 | 0.002786 | 0.007077 | 0.051703 | 0.002300 | 0.003991 | 0.003324 | 0.030737 | 0.002697 | 0.003201 | 0.004257 | 0.040556 |
Cell Periphery | 0.000842 | 0.000061 | 0.000072 | 0.000199 | 0.000106 | 0.000464 | 0.000037 | 0.000211 | 0.000141 | 0.000200 | 0.000046 | 0.000206 |
Cytoplasm | 0.140129* | 0.006570 | 0.006836 | 0.109983* | 0.000935 | 0.005241 | 0.003950 | 0.074874 | 0.007571 | 0.006112 | 0.004667 | 0.091318 |
Cytoplasmic Foci | 0.143109 | 0.304216* | 0.402992* | 0.262879* | 0.212018* | 0.161952 | 0.341604* | 0.271089* | 0.208732* | 0.255212* | 0.356863* | 0.267244* |
Eisosomes | 0.001663 | 0.000049 | 0.000049 | 0.000072 | 0.000142 | 0.000165 | 0.000052 | 0.000084 | 0.000215 | 0.000089 | 0.000052 | 0.000079 |
Endoplasmic Reticulum | 0.000860 | 0.000446 | 0.004242 | 0.001195 | 0.000367 | 0.001000 | 0.001280 | 0.001165 | 0.000391 | 0.000637 | 0.002017 | 0.001179 |
Endosome | 0.143985 | 0.231569* | 0.195160 | 0.181828 | 0.239510* | 0.338253* | 0.201618* | 0.267346* | 0.234955* | 0.268317* | 0.200013* | 0.227293* |
Golgi | 0.021647 | 0.402801* | 0.284817* | 0.061074 | 0.466149* | 0.361629* | 0.393355* | 0.091500 | 0.444957* | 0.388619* | 0.366376* | 0.077250 |
Lipid Particles | 0.006456 | 0.013843 | 0.019914 | 0.010015 | 0.029539 | 0.006006 | 0.012260 | 0.028924 | 0.028439 | 0.011143 | 0.014162 | 0.020068 |
Mitochondria | 0.000244 | 0.004575 | 0.001629 | 0.008751 | 0.012091 | 0.021717 | 0.002163 | 0.009822 | 0.011526 | 0.010480 | 0.002030 | 0.009320 |
Mitotic Spindle | 0.002923 | 0.002694 | 0.029268 | 0.052522 | 0.001368 | 0.002598 | 0.007022 | 0.028277 | 0.001442 | 0.002661 | 0.012551 | 0.039632 |
None | 0.000058 | 0.000057 | 0.000016 | 0.001531 | 0.000041 | 0.000203 | 0.000011 | 0.007014 | 0.000042 | 0.000107 | 0.000012 | 0.004446 |
Nuclear Periphery | 0.000299 | 0.000019 | 0.000208 | 0.000313 | 0.000019 | 0.000094 | 0.000068 | 0.000410 | 0.000032 | 0.000045 | 0.000103 | 0.000365 |
Nuclear Periphery Foci | 0.000478 | 0.000479 | 0.006118 | 0.001501 | 0.000194 | 0.000211 | 0.001147 | 0.003791 | 0.000207 | 0.000386 | 0.002382 | 0.002719 |
Nucleolus | 0.000266 | 0.000078 | 0.000069 | 0.000328 | 0.000116 | 0.000488 | 0.000020 | 0.000889 | 0.000123 | 0.000219 | 0.000032 | 0.000626 |
Nucleus | 0.013323 | 0.000070 | 0.000513 | 0.002986 | 0.000429 | 0.000361 | 0.000113 | 0.004905 | 0.001043 | 0.000170 | 0.000213 | 0.004006 |
Peroxisomes | 0.013497 | 0.012916 | 0.005772 | 0.038797 | 0.019865 | 0.010255 | 0.005828 | 0.035349 | 0.019561 | 0.011999 | 0.005814 | 0.036964 |
Vacuole | 0.478651* | 0.008184 | 0.025072 | 0.192278 | 0.001296 | 0.055518 | 0.011677 | 0.125753 | 0.024055 | 0.024488 | 0.015007 | 0.156910 |
Vacuole Periphery | 0.002169 | 0.004145 | 0.003798 | 0.006142 | 0.001113 | 0.026964 | 0.002262 | 0.005470 | 0.001163 | 0.012005 | 0.002643 | 0.005785 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.72 | -3.69 | -3.97 | -1.91 | -0.66 | 8.22 | -0.07 | 3.52 | -3.67 | 3.13 | 6.35 | 0.54 | 3.48 | -3.18 | 2.81 |
Bud Neck | 1.76 | 1.74 | 1.01 | -11.29 | -12.24 | -2.14 | 1.03 | -8.03 | -5.24 | -9.14 | 0.29 | 1.71 | -8.35 | -11.35 | -15.02 |
Bud Site | 2.60 | 1.11 | -7.08 | -10.91 | -9.56 | -1.79 | -1.70 | -9.21 | -8.50 | -8.81 | -0.79 | -2.51 | -13.95 | -13.94 | -13.34 |
Cell Periphery | 1.73 | 1.70 | 1.48 | -1.65 | -1.48 | -1.69 | 3.67 | -2.14 | 1.27 | -4.31 | -0.77 | 3.38 | -0.88 | 0.28 | -3.92 |
Cytoplasm | 3.76 | 3.75 | 0.18 | -16.99 | -17.02 | -3.97 | -4.56 | -15.04 | -14.08 | -14.22 | 0.64 | 1.00 | -20.22 | -21.76 | -22.12 |
Cytoplasmic Foci | -4.94 | -7.83 | -2.29 | 9.90 | 17.73 | 6.95 | -19.50 | -5.01 | -10.87 | 11.05 | -7.26 | -23.45 | -4.12 | 2.87 | 19.29 |
Eisosomes | 1.51 | 1.51 | 1.50 | -2.23 | -2.01 | -0.38 | 6.04 | 3.72 | 1.43 | -3.58 | 2.24 | 3.11 | 2.69 | 0.75 | -3.88 |
Endoplasmic Reticulum | 1.89 | -3.94 | -1.30 | -4.05 | 3.65 | -3.20 | -3.76 | -5.10 | -0.89 | 0.51 | -1.85 | -5.71 | -6.19 | -4.01 | 3.09 |
Endosome | -3.36 | -1.97 | 0.03 | 10.85 | 5.93 | -8.80 | 4.99 | -0.02 | 8.39 | -4.57 | -4.09 | 4.80 | 4.93 | 10.57 | 1.00 |
Golgi | -25.50* | -16.22 | -1.61 | 44.47* | 23.68* | 7.89 | 7.91 | 41.58* | 23.74 | 41.22* | 5.62 | 8.56 | 43.57* | 48.15* | 51.00* |
Lipid Particles | -2.94 | -4.53 | -1.51 | 2.28 | 4.17 | 6.56 | 4.70 | 0.56 | -7.11 | -4.84 | 4.91 | 4.06 | 2.64 | -3.86 | -2.37 |
Mitochondria | -4.59 | -1.68 | -3.44 | -0.88 | -2.42 | -3.09 | 4.99 | 1.51 | 4.60 | -4.10 | 0.51 | 5.09 | 1.91 | 1.91 | -4.41 |
Mitotic Spindle | 0.25 | -6.95 | -9.21 | -9.40 | -3.56 | -0.83 | -4.56 | -8.07 | -7.37 | -6.40 | -1.54 | -8.19 | -12.44 | -12.05 | -8.49 |
None | 0.03 | 1.12 | -1.98 | -1.98 | -2.05 | -0.98 | 1.94 | -3.05 | -2.95 | -3.07 | -1.04 | 2.00 | -3.45 | -3.38 | -3.48 |
Nuclear Periphery | 3.57 | 0.83 | -2.88 | -8.69 | -4.74 | -2.86 | -2.22 | -6.65 | -5.42 | -5.76 | -0.97 | -3.58 | -10.16 | -9.93 | -8.22 |
Nuclear Periphery Foci | 0.05 | -4.19 | -5.99 | -6.10 | 2.79 | -0.01 | -4.23 | -4.99 | -5.00 | -3.53 | -1.50 | -5.70 | -7.12 | -6.68 | -1.21 |
Nucleolus | 3.38 | 3.85 | -2.01 | -5.44 | -6.09 | -0.88 | 1.28 | -2.75 | -1.26 | -3.07 | -0.56 | 1.24 | -3.10 | -2.34 | -3.74 |
Nucleus | 2.32 | 2.22 | 1.18 | -7.19 | -6.42 | 0.61 | 1.03 | -4.43 | -4.85 | -5.01 | 2.08 | 1.84 | -5.56 | -7.80 | -7.65 |
Peroxisomes | 0.05 | 1.58 | -3.35 | -5.79 | -8.79 | 3.55 | 6.00 | -3.73 | -6.91 | -9.03 | 3.23 | 6.28 | -4.40 | -8.78 | -12.40 |
Vacuole | 8.19 | 7.87 | 3.32 | -26.45 | -24.03 | -9.34 | -7.67 | -20.67 | -11.50 | -18.82 | 0.01 | 1.56 | -24.83 | -28.50 | -30.42 |
Vacuole Periphery | -2.36 | -2.15 | -2.44 | -0.36 | -0.77 | -10.04 | -4.04 | -5.24 | 8.36 | -3.58 | -10.58 | -5.40 | -6.41 | 6.50 | -3.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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