Standard name
Description Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 – 0 0 0 0 0 0.23 0.15 0.09 0 0 0 0 0 0 0 0 0
Bud – – 0 0.05 0.05 0 – 0.06 0.11 0.06 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – – 0.1 0.09 0.08 0 – 0 0 0 0 0 0.08 0.26 0.58 0.68 0.35 0.41 0 0.05 0 0 0 0
Endoplasmic Reticulum – – 0 0 0 0 – 0 0 0 0 0 0.31 0.15 0.11 0.05 0 0 0 0 0 0 0 0
Endosome – – 0.4 0.52 0.49 0.3 – 0.21 0.06 0.06 0 0.08 0.19 0.22 0.12 0.05 0.25 0.25 0.22 0.28 0.4 0.38 0.33 0.23
Golgi – – 0.13 0.18 0.19 0.09 – 0 0 0 0 0 0.08 0.05 0 0 0.05 0.06 0.25 0 0.38 0.25 0.24 0.14
Mitochondria – – 0.3 0.29 0.22 0.57 – 0.58 0.81 0.84 0.85 0.83 0.13 0.07 0 0.05 0.1 0.1 0.14 0.24 0.05 0.15 0.11 0.09
Nucleus – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0 0 0.05 – 0.09 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0.08 0.08 – 0.21 0.13 0.1 0.09 0.14 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0.08 0.09 0.06 – 0.14 0.08 0.06 0.08 0.07 0.13 0.15 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – – 0.27 0.09 0.05 0 – 0 0 0 0 0 0.06 0.06 0.07 0.4 0.44 0.46 0.28 0.29 0.1 0.12 0.23 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 1 6 15 4 – 10 8 2 4 7 14 27 15 0 1 2 0 1 5 0 2 2
Bud – – 5 12 20 18 – 35 46 39 33 38 0 4 3 0 1 3 0 0 0 0 0 1
Bud Neck – – 0 0 0 0 – 0 0 0 1 0 0 2 4 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 – 5 4 4 5 6 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 – 0 2 1 1 0 0 2 3 0 1 0 0 0 0 0 0 0
Cytoplasm – – 32 21 31 13 – 15 8 4 6 6 5 46 94 43 55 58 1 2 0 0 3 2
Endoplasmic Reticulum – – 12 3 10 4 – 2 3 2 1 2 19 26 17 3 5 5 0 0 1 2 3 7
Endosome – – 128 125 182 145 – 135 24 38 16 42 12 40 19 3 39 36 15 13 159 32 75 60
Golgi – – 43 44 71 43 – 22 9 8 4 6 5 9 2 0 8 9 18 1 150 21 56 37
Mitochondria – – 96 70 81 275 – 364 347 545 323 433 8 13 4 3 16 14 9 10 20 12 26 23
Nucleus – – 0 0 0 0 – 2 0 0 0 0 0 1 2 4 0 0 2 0 0 0 0 0
Nuclear Periphery – – 3 0 1 1 – 3 2 5 1 5 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus – – 1 7 5 26 – 56 26 41 13 20 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes – – 5 4 28 37 – 135 55 66 34 75 0 0 0 0 0 0 1 0 13 1 7 1
SpindlePole – – 13 20 32 29 – 87 35 40 31 35 8 27 6 0 4 3 0 0 1 0 0 0
Vac/Vac Membrane – – 87 22 20 16 – 10 5 5 3 7 4 10 12 25 69 66 19 13 39 10 52 122
Unique Cell Count 323 241 369 480 631 430 645 381 522 62 178 161 63 158 142 71 46 394 85 233 265
Labelled Cell Count 426 334 496 611 881 574 800 476 682 75 207 181 81 200 197 71 46 394 85 233 265


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 6.7 7.7 7.7 7.2 – 7.3 7.1 6.6 6.7 7.1 6.8 6.5 6.2 5.6 7.0 8.2 7.4 7.6 7.5
Std Deviation (1e-4) – – 1.2 1.7 1.6 1.2 – 1.3 1.4 1.3 1.4 1.3 0.9 1.0 0.9 1.0 1.4 1.6 1.3 1.5 1.8
Intensity Change (Log2) – – – 0.2 0.21 0.1 – 0.13 0.09 -0.02 0.01 0.08 0.02 -0.04 -0.11 -0.25 0.07 0.29 0.14 0.18 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 3.3 0 – 0 0 0 0 0 0 6.9 5.2 0 0 0
Bud 2.4 2.7 1.8 – 2.9 4.9 3.2 4.2 3.7 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.7 -4.3 – -5.1 -4.9 -7.2 -4.9 -6.0 -0.5 4.7 11.5 10.7 6.7 7.8
Endoplasmic Reticulum -1.8 -0.8 -2.9 – 0 -2.9 0 -3.4 -3.7 0 4.4 3.0 0 -0.3 -0.1
Endosome 2.9 2.6 -2.8 – -6.0 -11.5 -13.1 -11.6 -11.1 -3.0 -3.9 -6.3 -5.3 -3.2 -3.0
Golgi 1.6 2.1 -2.0 – -5.7 -6.0 -7.9 -6.5 -7.4 -1.1 -2.9 -4.3 -3.1 -2.8 -2.2
Mitochondria -0.2 -2.3 7.7 – 8.2 14.1 17.0 14.8 15.5 -2.7 -5.8 -7.0 -4.1 -4.8 -4.6
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.9 – 5.3 4.2 4.4 2.9 3.2 0 0 0 0 0 0
Peroxisomes 0 3.7 3.8 – 8.2 5.6 4.9 4.3 6.2 0 0 0 0 0 0
SpindlePole 2.1 2.5 1.3 – 4.7 2.3 1.4 2.2 1.6 0 4.4 -0.2 0 -0.8 0
Vacuole -5.3 -7.8 -9.8 – -12.3 -10.7 -13.1 -10.4 -11.5 -3.5 -5.8 -5.0 2.0 3.7 4.1