Standard name
Description Subunit a of the vacuolar-ATPase V0 domain; one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.09 0 0 0 0 0 0 – 0 0 0 0.11 0.12 0 0 0.17 0.06 0 0.05 0 0 0 0
Bud 0 0 0 0.06 0 0 0.07 0.08 – 0.07 0.07 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.09 0.09 0.11 0.15 0.11 0.1 0.09 – 0.07 0.05 0.05 0.11 0.12 0.14 0.26 0.36 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0.35 0 0.43 0.35 0.36 0 0 0.09 – 0 0 0 0.11 0.12 0.35 0.36 0.22 0.33 0.33 0.31 0.36 0.26 0.31 0.29
Golgi 0.06 0.06 0.05 0.06 0.05 0 0 0 – 0 0 0 0 0.15 0.22 0 0.05 0.08 0.3 0.22 0.28 0.25 0.29 0.32
Mitochondria 0.49 0.83 0.33 0.55 0.44 0.86 0.82 0.79 – 0.9 0.88 0.91 0.72 0.58 0.28 0.12 0.08 0.16 0.15 0.24 0.08 0.18 0.18 0.17
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0.17 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0 0.18 0.08 0.05 0 0.08 0 – 0 0 0 0 0.08 0 0.05 0 0 0.11 0.1 0.19 0.19 0.09 0.08
SpindlePole 0.1 0 0.2 0.07 0.07 0 0.07 0.1 – 0 0 0 0.11 0 0.07 0.23 0.14 0.2 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0.07 0.07 0 0 0 – 0 0.05 0 0 0 0.06 0.14 0.12 0.08 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 13 6 0 1 1 0 1 – 1 5 0 2 3 1 4 10 3 10 8 6 1 4 5
Bud 9 3 10 12 9 9 24 23 – 20 19 25 0 0 0 4 0 3 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 2 0 0 2 3 1 – 4 3 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 – 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 33 12 23 22 33 32 32 26 – 22 14 17 2 3 13 62 21 17 3 1 3 0 1 0
Endoplasmic Reticulum 10 9 3 0 1 3 3 1 – 5 1 5 0 1 4 16 2 2 0 0 2 0 2 0
Endosome 128 5 109 72 76 10 7 26 – 4 4 10 2 3 33 87 13 17 152 47 115 14 42 44
Golgi 23 8 13 12 10 1 0 0 – 1 3 0 0 4 21 4 3 4 138 34 91 13 40 48
Mitochondria 178 116 83 113 95 240 270 222 – 268 227 306 13 15 26 30 5 8 69 36 25 10 25 25
Nucleus 2 0 0 0 1 3 1 2 – 0 2 0 1 0 0 0 2 3 0 0 0 0 0 0
Nuclear Periphery 0 3 1 0 0 0 0 0 – 2 2 1 0 0 0 1 1 0 0 0 1 0 0 0
Nucleolus 2 0 0 1 3 6 6 7 – 6 4 2 3 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 29 0 45 16 10 6 26 9 – 7 6 2 0 2 4 11 0 0 50 15 62 10 12 12
SpindlePole 36 6 51 14 14 11 24 28 – 11 11 10 2 0 7 56 8 10 14 3 3 0 1 1
Vac/Vac Membrane 31 5 7 15 16 11 12 6 – 7 12 15 0 1 6 35 7 4 14 4 3 0 5 7
Unique Cell Count 361 139 252 204 214 279 328 280 297 257 336 18 26 94 243 59 51 464 155 322 55 139 151
Labelled Cell Count 490 180 353 277 269 335 408 352 359 316 398 25 32 117 311 72 71 464 155 322 55 139 151


Early Golgi, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 5.3 5.9 5.8 5.6 4.9 4.6 4.9 – 4.4 4.7 4.7 6.2 6.7 7.3 5.5 6.2 6.6 5.6 7.4 8.0
Std Deviation (1e-4) 0.8 0.7 1.6 1.4 1.6 1.5 1.3 1.5 – 1.0 1.0 1.0 1.0 1.0 1.4 1.1 1.1 1.1 0.7 2.0 2.5
Intensity Change (Log2) – – – -0.03 -0.06 -0.26 -0.37 -0.26 – -0.41 -0.32 -0.32 0.09 0.19 0.31 -0.1 0.07 0.15 -0.06 0.33 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0.9 0.1 -0.5 0 2.0 – 0 0 0 0 0 0 -1.6 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 2.1 0.9 0.3 0.1 – -0.7 -1.6 -1.9 0 0 1.3 4.8 5.3 4.7
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 3.1 0 0
Endosome -1.7 -1.7 -10.9 0 -9.0 – 0 0 0 -2.7 -3.1 -1.4 -1.7 -3.0 -1.3
Golgi 0.3 -0.2 -3.4 0 -3.8 – 0 0 0 0 0 4.8 -2.1 0 0
Mitochondria 4.8 2.5 12.5 0 10.8 – 0 0 0 3.4 2.5 -0.9 -5.5 -3.8 -2.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -3.1 -4.4 -6.1 0 -5.6 – 0 0 0 0 0 -3.2 -4.7 -3.5 -3.3
SpindlePole -4.1 -4.3 -5.8 0 -3.3 – 0 0 0 0 0 -2.8 0.8 -1.2 -0.1
Vacuole 2.3 2.3 0.7 0.6 -0.5 – -0.3 1.1 1.1 0 0 0 4.6 0 0