Standard name
Description Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0.05 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.10 0.28 0.18 0.19 0.11 0.07 0.06 0 0.05 0.05 0 0 0.61 0.64 0.78 0.74 0.84 0.84 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.06 0.09 0.05 0 0 0 0 0 0
Endosome 0 0.08 0.11 0.09 0.06 0 0 0 0 0 0 0 0 0.10 0.05 0.05 0.05 0 0.05 0.09 0.09 0.11 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.84 0.57 0.70 0.77 0.82 0.92 0.93 0.94 0.96 0.94 0.95 0.96 0.10 0.11 0 0.10 0.07 0 0.73 0.66 0.69 0.65 0.68 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0
SpindlePole 0 0.05 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0 0.05 0 0 0 0 0 0 0 0 0.12 0.09 0 0 0 0 0.07 0 0.06 0.09 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 10 1 1 1 2 2 7 9 9 2 10 10
Bud 10 5 11 8 12 26 32 29 30 29 22 25 5 1 2 1 1 1 0 4 1 0 4 4
Bud Neck 1 2 0 0 0 0 2 0 0 2 0 0 4 1 5 1 3 5 2 3 4 3 2 10
Bud Site 9 0 2 0 1 8 10 6 8 15 5 12 0 0 0 0 0 1
Cell Periphery 6 11 2 5 3 2 1 7 1 5 1 4 7 3 2 5 1 2 0 1 0 0 0 1
Cytoplasm 26 79 55 43 36 35 38 23 29 31 19 23 101 126 202 124 92 98 2 19 4 2 5 12
Endoplasmic Reticulum 1 7 2 0 8 7 6 1 4 0 3 2 14 8 8 10 10 6 1 1 1 1 7 4
Endosome 6 22 35 20 19 3 0 2 1 0 0 1 7 19 12 9 6 2 13 33 31 22 18 15
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 4 0 2 2 0 1 12 6 3 6 5 6
Mitochondria 222 161 218 176 267 449 566 621 552 556 443 581 17 21 5 16 8 5 209 247 236 131 215 220
Nucleus 4 0 0 1 4 14 20 18 17 17 5 9 1 1 2 1 0 1 0 1 0 0 2 1
Nuclear Periphery 4 3 0 0 1 5 9 3 3 8 7 6 0 0 0 0 1 0 2 0 2 3 1 1
Nucleolus 4 0 2 0 1 6 5 10 8 6 6 10 0 0 0 0 0 1 1 1 1 3 0 1
Peroxisomes 4 2 55 4 8 2 3 5 8 7 1 2 0 7 4 2 0 0 13 4 24 5 7 8
SpindlePole 11 13 20 16 15 14 24 28 24 26 17 14 6 7 10 10 0 3 3 6 6 1 4 6
Vac/Vac Membrane 9 30 13 6 17 13 17 20 16 21 14 8 6 24 23 3 1 5 8 26 4 12 29 41
Unique Cell Count 263 282 310 230 326 487 607 660 575 594 468 606 165 198 258 167 110 116 289 376 341 202 319 354
Labelled Cell Count 318 337 415 279 392 584 733 773 701 723 543 697 182 219 278 185 125 133 289 376 341 202 319 354

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Mitochondria

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.5 3.9 3.8 3.6 3.7 3.1 3.3 3.1 3.2 3.1 3.2 5.3 5.1 5.2 5.4 5.0 5.1 6.0 6.1 6.1
Std Deviation (1e-4) 1.6 0.9 0.9 1.2 0.8 1.0 0.8 0.9 0.7 0.8 0.7 0.7 0.7 1.0 1.0 1.8 1.5 1.6 1.4 1.6 1.6
Intensity Change (Log2) -0.07 -0.13 -0.1 -0.36 -0.27 -0.33 -0.32 -0.33 -0.29 0.43 0.37 0.41 0.44 0.35 0.37 0.61 0.62 0.63

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 0