Standard name
Description Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.10 0.09 0.07 0.12 0.21 0.41 0.48 0.43 0.45 0.50 0.51 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.91 0.88 0.96 0.89 0.84 0.83 0.81 0.71 0.83 0.76 0.78 0.90 0.86 0.89 0.84 0.88 0.88 0.84 0.85 0.93 0.79 0.88 0.86
Cytoplasm 0.10 0.13 0 0.06 0.08 0.10 0.12 0.09 0.10 0.14 0.10 0 0 0.05 0.21 0.14 0.13 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.08 0 0 0.09 0.06 0.07 0.09 0.08 0.10 0.07 0 0 0.07 0.11 0 0 0.11 0.06 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 0.06 0.11 0.14 0.14 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 20 15 20 38 86 98 108 84 114 89 14 4 5 24 6 4 0 2 1 5 5 5
Bud 2 8 2 3 5 6 11 13 9 11 5 0 0 1 9 9 3 0 3 3 5 1 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 2
Bud Site 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 75 199 215 152 154 174 165 180 156 175 137 458 300 286 339 289 254 87 289 262 287 323 387
Cytoplasm 8 29 7 10 15 21 24 22 18 32 18 15 13 15 83 45 38 3 18 2 8 4 6
Endoplasmic Reticulum 3 8 2 7 15 4 8 22 11 17 15 39 35 21 13 6 20 11 12 0 40 20 21
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 0 0 2 0 0 0 2 0 0 1 1 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 2 13 7 14 21 32 24 0 0 0 21 13 14 1 8 7 8 5 11
Nucleus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 1 0 0 1 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 1 4 1 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 1 0 0 0 2 0 0 0 1 0 3 0 0 2 0 1 0 0
Unique Cell Count 82 225 225 170 184 210 203 253 187 230 176 511 348 320 402 330 288 105 341 281 363 366 449
Labelled Cell Count 96 264 243 193 236 307 317 372 299 384 288 530 357 331 490 373 336 105 341 281 363 366 449

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Bud, Cell Periphery, Endoplasmic Reticulum

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.1 58.3 55.3 47.7 35.2 31.0 30.4 31.9 32.4 31.2 31.7 75.7 79.7 81.7 41.6 52.6 59.9 39.8 41.5 46.6
Std Deviation (1e-4) 9.7 15.1 12.7 12.0 12.5 11.6 11.9 10.6 11.2 11.2 10.3 18.2 20.2 19.5 14.0 16.3 19.8 11.2 12.5 13.3
Intensity Change (Log2) -0.21 -0.65 -0.84 -0.86 -0.79 -0.77 -0.83 -0.8 0.45 0.53 0.56 -0.41 -0.07 0.11 -0.48 -0.41 -0.25

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 4.2 8.5 9.8 9.0 9.0 10.2 10.0 -2.5 -3.6 -3.1 -0.3 -2.9 -3.1
Bud 0 0 0 2.7 2.7 0 2.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.4 -4.0 -4.3 -4.7 -7.0 -4.1 -5.9 -5.4 -2.6 -3.6 -2.6 -4.2 -3.2 -3.0
Cytoplasm 0 0 1.5 2.3 1.0 1.5 2.5 0 0 0.6 0 4.6 3.2 2.5
Endoplasmic Reticulum 0 3.7 0 0 3.9 2.9 3.5 3.8 3.7 4.4 3.2 1.8 0 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 0 3.6 5.2 5.8 5.7 0 0 0 3.5 3.0 3.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0