Standard name
Description Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.05 0 0 0 0 0.09 0.14 0.18 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0 0 0 0 0 0.06 0.23 0.11 0.39 0.16 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.94 0.89 0.92 0.92 0.91 0.84 0.84 0.78 0.85 0.75 0.77 0.89 0.81 0.77 0.65 0.80 0.83 0.76 0.85 0.77 0.77
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0.14 0.05 0 0 0.13 0.10 0.15 0.11 0.22 0.21 0 0.09 0.07 0.17 0.14 0.11 0.10 0.07 0.09 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Bud 0 0 0 0 0 0 1 3 1 6 1 0 0 0 0 0 2 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 0 1 2 4 4 10 7 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 7 5 5 8 15 17 8 9 5 10 16 34 52 6 0 0 1 2 6 3
Endoplasmic Reticulum 0 1 1 0 0 0 2 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Endosome 0 1 3 1 2 4 4 0 1 0 2 2 2 2 0 1 2 2 1 3 7
Golgi 0 0 3 0 2 0 0 0 0 0 0 0 0 2 1 1 2 1 0 0 0
Mitochondria 18 7 0 1 3 10 21 53 39 128 48 0 2 0 2 0 2 1 1 2 1
Nucleus 100 334 148 134 208 228 301 182 300 249 229 159 198 229 26 87 346 133 139 196 212
Nuclear Periphery 0 0 0 1 2 3 1 1 5 2 3 0 1 1 0 0 1 0 0 0 0
Nucleolus 31 51 8 6 9 36 36 36 38 72 63 5 21 21 7 15 45 18 11 23 21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0
SpindlePole 0 1 7 1 4 3 6 4 10 3 9 1 2 5 1 0 3 2 0 5 7
Vac/Vac Membrane 1 10 4 1 3 1 7 2 1 0 4 2 1 1 2 1 7 8 3 10 11
Unique Cell Count 106 374 161 145 229 273 358 233 352 330 296 178 244 297 40 110 418 175 164 257 277
Labelled Cell Count 150 412 179 152 241 301 398 293 408 476 376 185 261 317 47 110 418 175 164 257 277

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.9 7.7 7.6 7.6 6.3 6.7 5.7 5.6 4.7 5.4 8.1 8.2 7.9 9.2 7.1 7.7 7.9
Std Deviation (1e-4) 0.8 1.2 1.6 2.2 1.9 1.7 2.7 2.1 1.1 0.8 1.4 1.7 1.9 1.9 2.9 1.0 1.7 1.5
Intensity Change (Log2) -0.01 -0.02 -0.28 -0.2 -0.43 -0.45 -0.7 -0.52 0.08 0.09 0.04 0.26 -0.11 0.0 0.04

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 6.5 4.4 0 5.4 0 0 0 0
Nucleus 0 0 0 0 0 0 0 3.0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 -0.5 2.7 1.9 3.2 2.1 0 4.6 -1.0 1.4 0.9 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 -0.9 0 -0.7 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0