Standard name
Human Ortholog
Description Mitochondrial ornithine acetyltransferase; catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0.06 0.09 0.1 0.05 0.06 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.18 0 0 0 0 0 0 0 0 0 0 0.12 0.32 0.35 0.35 0.35 0.31 0.24 0.22 0.12 0.29 0.32 0.34
Mitochondria 1.0 0.84 0.95 0.97 0.95 0.95 0.95 0.9 0.93 0.94 0.93 0.92 0.87 0.75 0.74 0.08 0.14 0.12 0.64 0.62 0.66 0.47 0.49 0.44
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.08 0.16 0.12 0.19 0.25 0.24 0.27 0.22 0.21 0.22 0.19 0.12 0.08 0 0.26 0.27 0.3 0 0 0 0.07 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.12 0.1 0.06 0.06 0.06 0 0 0 0 0 0 0.18 0.24 0.19 0.33 0.2 0.16 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 2 0 2 2 1 0 0 1 2 11 7 8 4 9 21 4 12 11
Bud 0 0 1 0 0 0 1 3 4 4 1 1 1 0 1 0 0 0 0 0 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 1 2 0 3 0 0 0 3 0 0 0 1 0 0 4 1 1 1 0 2 0 0 0
Cytoplasm 1 0 2 1 3 1 1 3 3 3 1 0 1 0 2 8 9 3 0 0 0 1 0 0
Endoplasmic Reticulum 0 0 1 0 0 1 0 1 3 3 1 2 1 0 0 4 11 10 0 0 1 0 4 2
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0
Golgi 0 13 7 3 9 5 1 2 5 1 4 6 9 37 51 55 55 33 18 21 25 25 67 77
Mitochondria 71 61 191 169 250 268 412 330 341 425 275 334 68 86 108 13 22 13 50 61 140 40 103 98
Nucleus 0 0 1 0 0 0 1 0 0 0 2 1 0 1 0 2 1 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 2 0 2 3 7 6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 17 6 33 21 51 69 103 98 80 95 64 68 9 9 5 41 43 31 0 1 0 5 6 13
Peroxisomes 0 0 0 0 2 0 1 0 0 0 0 1 0 0 0 2 1 3 2 0 16 0 1 1
SpindlePole 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2
Vac/Vac Membrane 8 9 21 11 17 16 10 12 11 9 9 16 14 28 27 52 32 17 0 2 0 3 6 10
Unique Cell Count 71 73 202 174 262 281 434 367 367 453 296 363 78 115 145 157 159 105 79 99 213 87 210 225
Labelled Cell Count 97 90 260 205 335 362 535 451 455 545 366 435 105 162 196 193 182 119 79 99 213 87 210 225


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 72.9 91.1 86.3 77.0 66.3 56.5 47.3 45.4 40.7 39.2 35.4 37.9 84.7 78.7 72.8 104.5 104.9 99.5 76.4 85.7 89.0
Std Deviation (1e-4) 19.0 20.9 25.3 19.3 19.2 17.5 15.0 14.0 13.5 13.8 13.4 14.2 20.6 18.6 20.2 46.1 43.6 39.5 27.1 25.7 28.3
Intensity Change (Log2) -0.16 -0.38 -0.61 -0.87 -0.93 -1.09 -1.14 -1.29 -1.19 -0.03 -0.13 -0.25 0.28 0.28 0.2 -0.18 -0.01 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 -1.2 0 0 0 0 0 0 0 7.1 7.8 7.8 7.8 6.9
Mitochondria 1.2 0.4 0.4 0.2 -1.9 -0.8 -0.4 -0.7 -1.1 -2.1 -5.1 -5.3 -16.4 -15.5 -14.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.2 0.9 2.2 2.1 2.8 1.6 1.4 1.5 0.7 -1.0 -2.1 -3.8 2.3 2.5 2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.4 -1.5 -1.9 -4.4 -3.5 -3.7 -4.8 -3.4 -2.8 1.7 3.3 2.2 5.3 2.6 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 118.0867 144.5445 119.4171 142.542 120.7466 132.5704 117.6524 151.7708 133.6243 113.3306 131.9527 140.5985 137.0692 146.5553 136.4058 136.0141 141.1092 138.05
Actin 0.0026 0.0002 0.0043 0.0003 0.0005 0 0.0021 0.0012 0.0003 0.0004 0.0001 0.0009 0.0398 0.0008 0.0009 0.0007 0.0007 0.0001
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Bud Neck 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0018 0.0001 0 0 0 0
Bud Periphery 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0
Bud Site 0.0001 0 0.0001 0 0 0 0.0003 0 0 0.0001 0 0 0.0005 0.0002 0 0 0 0
Cell Periphery 0.0001 0.0001 0.0002 0 0 0 0.0003 0.0004 0.0002 0.0051 0.0001 0.0002 0.0002 0.0007 0.0003 0.0001 0.0001 0
Cytoplasm 0 0 0.0005 0 0 0 0 0 0 0 0 0 0.0008 0 0.0005 0 0 0
Cytoplasmic Foci 0.0024 0 0.0005 0 0.0001 0 0.0004 0 0.0002 0 0 0.0003 0.0041 0 0.0002 0 0.0009 0
Eisosomes 0.0046 0.0139 0.0032 0.0027 0.0002 0.0049 0.0095 0.0287 0.0086 0.0193 0.0025 0.0061 0.002 0.0117 0.0031 0.0013 0.0028 0.0008
Endoplasmic Reticulum 0 0 0.0019 0 0 0 0 0 0 0 0 0 0.0013 0 0.0003 0 0 0
Endosome 0.0003 0 0.0042 0 0.0002 0 0 0 0 0 0 0.0001 0.0058 0 0.0005 0 0 0
Golgi 0.0023 0.0001 0.0018 0 0.0005 0 0.0002 0.0002 0 0 0 0.0004 0.0028 0.0001 0.0002 0.0003 0.0001 0
Lipid Particles 0.0337 0.0044 0.0042 0.0004 0.0038 0.0001 0.0023 0.0018 0.001 0.001 0.0002 0.0036 0.008 0.0043 0.0044 0.0033 0.0007 0.0009
Mitochondria 0.9485 0.9761 0.9599 0.9955 0.9923 0.9945 0.9719 0.9592 0.9853 0.9438 0.9961 0.9782 0.8925 0.9688 0.9729 0.9909 0.9306 0.9956
None 0 0 0.0058 0 0 0 0.0001 0 0 0 0 0 0.0022 0 0.0005 0 0.0001 0
Nuclear Periphery 0 0 0.0045 0 0 0 0 0 0 0 0 0.0001 0.0054 0 0.0028 0 0 0
Nucleolus 0.0001 0.0001 0.0008 0 0 0 0.0024 0.0005 0.0011 0.0008 0 0.0009 0.0145 0.0048 0.001 0.0001 0.061 0
Nucleus 0 0 0.0005 0 0 0 0.0003 0.0001 0 0.0001 0 0.0001 0.001 0.0003 0.0036 0 0.001 0
Peroxisomes 0.0045 0.0007 0.0047 0.0007 0.001 0.0002 0.0092 0.0019 0.0024 0.0016 0.0003 0.0015 0.0072 0.0019 0.001 0.0006 0.0011 0.0002
Punctate Nuclear 0.0001 0 0.0009 0 0 0 0 0 0 0 0 0 0.0035 0 0.0003 0 0 0
Vacuole 0.0001 0.0001 0.0004 0 0.0002 0 0.0002 0.0004 0.0001 0.0035 0 0.0003 0.0005 0.0005 0.0011 0.0001 0.0001 0
Vacuole Periphery 0.0004 0.0042 0.0012 0.0003 0.001 0.0002 0.0006 0.0055 0.0008 0.0241 0.0005 0.0073 0.0055 0.0058 0.0062 0.0025 0.0008 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.0812 62.4854 48.5748 54.5745 71.7464 71.5236 75.213 78.4299 76.7124 77.5973
Translational Efficiency 1.3498 1.3307 1.4744 1.3537 1.3902 1.5809 1.3629 1.258 1.2528 1.3088

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
34 192 2650 537 2046 2064 2134 24 2080 2256 4784 561

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1598.54 6523.53 4416.42 2138.93 4472.65 5996.66 3824.49 2488.89 4425.67 6041.50 4152.38 2153.90
Standard Deviation 1021.43 2137.21 1221.25 713.30 1452.58 1892.81 1087.84 660.17 1491.77 1920.46 1200.25 714.63
Intensity Change Log 2 2.028898 1.466123 0.420134 0.423029 -0.225862 -0.845627 1.044205 0.440824 -0.391647

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003798 0.009422 0.000874 0.010238 0.002026 0.005373 0.001776 0.029163 0.002055 0.005717 0.001276 0.011047
Bud Neck 0.002575 0.004880 0.000901 0.005801 0.003787 0.001607 0.001433 0.009246 0.003767 0.001885 0.001138 0.005949
Bud Site 0.015609 0.002909 0.000973 0.016143 0.008838 0.001473 0.001869 0.012064 0.008949 0.001596 0.001373 0.015969
Cell Periphery 0.000694 0.000142 0.000083 0.003632 0.001635 0.000155 0.000118 0.000522 0.001620 0.000154 0.000099 0.003499
Cytoplasm 0.042256 0.000621 0.000114 0.001057 0.000790 0.000321 0.000294 0.001870 0.001468 0.000347 0.000194 0.001092
Cytoplasmic Foci 0.035647 0.050590 0.009066 0.014312 0.023593 0.022292 0.014202 0.023766 0.023790 0.024700 0.011357 0.014717
Eisosomes 0.000887 0.010594 0.001605 0.001568 0.004122 0.004641 0.001016 0.015227 0.004070 0.005147 0.001343 0.002153
Endoplasmic Reticulum 0.013049 0.000172 0.000018 0.000664 0.000146 0.000059 0.000036 0.000791 0.000357 0.000068 0.000026 0.000670
Endosome 0.016317 0.000889 0.000271 0.005868 0.000971 0.000372 0.000678 0.005588 0.001222 0.000416 0.000453 0.005856
Golgi 0.008745 0.006611 0.003474 0.026689 0.004415 0.009690 0.005245 0.026650 0.004485 0.009428 0.004264 0.026688
Lipid Particles 0.041030 0.007493 0.004254 0.002958 0.005463 0.007963 0.002378 0.001186 0.006044 0.007923 0.003417 0.002882
Mitochondria 0.382892* 0.859556* 0.951742* 0.817258* 0.871627* 0.910383* 0.941534* 0.815200* 0.863638* 0.906058* 0.947189* 0.817170*
Mitotic Spindle 0.040038 0.003265 0.000696 0.003632 0.000605 0.000676 0.001180 0.001634 0.001250 0.000896 0.000912 0.003547
None 0.014164 0.004791 0.004696 0.003197 0.007488 0.006240 0.003314 0.005035 0.007597 0.006117 0.004079 0.003276
Nuclear Periphery 0.013841 0.000372 0.000744 0.000923 0.000520 0.000410 0.000459 0.000555 0.000738 0.000407 0.000617 0.000908
Nuclear Periphery Foci 0.077834 0.000282 0.000199 0.000702 0.000340 0.000206 0.000253 0.000269 0.001607 0.000212 0.000223 0.000683
Nucleolus 0.020176 0.002078 0.000982 0.002599 0.004300 0.002071 0.001234 0.002000 0.004560 0.002072 0.001094 0.002573
Nucleus 0.197071* 0.000206 0.000080 0.000550 0.000605 0.000310 0.000128 0.000313 0.003817 0.000301 0.000102 0.000540
Peroxisomes 0.011679 0.022670 0.002792 0.008137 0.008741 0.008846 0.007748 0.018483 0.008789 0.010022 0.005003 0.008580
Vacuole 0.027003 0.007186 0.005047 0.038235 0.020028 0.007781 0.005448 0.011194 0.020142 0.007731 0.005226 0.037078
Vacuole Periphery 0.034696 0.005270 0.011388 0.035835 0.029959 0.009130 0.009657 0.019243 0.030037 0.008801 0.010616 0.035125

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.99 1.87 -2.20 -0.26 -4.04 -4.21 0.57 -1.29 -1.13 -1.31 -4.80 2.09 -3.71 -2.14 -4.08
Bud Neck -0.41 1.69 -0.90 -0.68 -5.61 3.52 3.79 -1.33 -1.90 -1.94 3.12 4.52 -2.10 -4.67 -5.65
Bud Site 1.23 1.40 0.08 -3.61 -4.18 5.23 4.92 -0.51 -1.85 -1.78 5.31 5.56 -1.85 -4.13 -4.20
Cell Periphery 2.33 2.54 -1.99 -2.48 -2.52 4.56 4.68 2.97 -1.81 -2.00 4.59 4.78 -1.35 -2.48 -2.52
Cytoplasm 2.58 2.61 2.56 -1.40 -3.87 2.20 2.39 -0.73 -1.16 -1.19 3.19 3.60 1.25 -2.98 -3.73
Cytoplasmic Foci -1.12 2.40 1.88 3.86 -1.97 0.53 4.32 0.00 -0.13 -0.99 -0.40 6.49 3.09 3.48 -1.31
Eisosomes -5.37 -1.69 -1.54 5.08 0.16 -1.09 11.72 -0.81 -0.77 -1.03 -2.28 10.21 2.86 4.07 -1.30
Endoplasmic Reticulum 3.53 3.57 3.39 -2.39 -3.32 3.68 4.62 -1.08 -1.23 -1.26 3.99 4.60 -1.57 -3.20 -3.42
Endosome 2.68 2.79 1.75 -3.78 -4.33 4.78 1.83 -1.19 -1.35 -1.27 5.05 4.82 -3.69 -4.36 -4.33
Golgi 0.79 2.24 -3.92 -4.78 -5.76 -5.08 -0.98 -1.84 -1.40 -1.77 -5.10 0.39 -5.70 -4.35 -5.77
Lipid Particles 1.94 2.15 2.22 2.17 1.52 -2.51 4.73 6.28 7.73 2.73 -1.91 3.70 3.36 4.96 0.72
Mitochondria -6.25 -7.62 -5.78 1.98 10.63 -5.75 -10.93 1.05 1.79 2.39 -6.17 -13.88 3.44 6.99 10.61
Mitotic Spindle 1.80 1.95 1.80 -0.05 -1.66 -0.31 -1.39 -1.33 -0.98 -0.52 0.38 0.69 -1.34 -1.46 -1.56
None 1.98 2.01 2.31 2.36 3.68 2.89 11.90 1.53 0.76 -1.09 3.53 10.06 8.92 6.44 2.15
Nuclear Periphery 2.01 1.95 1.92 -2.17 -0.68 1.55 0.65 -0.16 -0.67 -0.43 2.49 0.80 -0.68 -2.15 -1.21
Nuclear Periphery Foci 2.50 2.50 2.49 -1.70 -2.19 2.64 0.84 0.86 -0.84 -0.12 2.56 2.53 1.55 -2.14 -2.06
Nucleolus 2.50 2.66 2.41 -0.74 -2.43 3.38 5.17 2.56 0.10 -1.03 3.83 5.98 2.28 -0.75 -2.32
Nucleus 3.96 3.97 3.96 -1.93 -2.62 1.93 3.76 1.98 0.15 -1.53 3.60 3.83 3.34 -1.19 -2.57
Peroxisomes -1.26 1.14 0.43 3.01 -2.88 -0.08 0.74 -1.11 -1.10 -1.23 -0.92 3.52 0.10 0.73 -1.95
Vacuole 2.52 2.89 -1.23 -6.20 -7.35 7.07 8.70 1.86 -0.73 -1.24 7.32 9.42 -3.71 -6.73 -7.37
Vacuole Periphery 2.36 1.86 -0.19 -7.01 -5.78 8.60 8.15 1.01 -0.97 -0.92 8.92 8.21 -1.13 -6.49 -6.06